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Molecular epidemiology, characterization of virulence factors and antibiotic resistance profile of Streptococcus agalactiae isolated from dairy farms in China and Pakistan

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Journal of Integrative Agriculture 2023年第5期22卷 1514-1528页

作者： Ambreen LEGHARI Shakeel Ahmed LAKHO Faiz muhammad KHAND Khaliq ur Rehman BHUTTO Sameen Qayoom LONE muhammad tahir aleem Iqra BANO muhammad Ali CHANDIO Jan muhammad SHAH LIN Hui-xing FAN Hong-jie MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary MedicineNanjing Agricultural UniversityNanjing 210095P.R.China Shaheed Benazir Bhutto University of Veterinary and Animal Sciences Sakrand 67210Pakistan Central Veterinary Diagnostic Laboratory Tando Jam 70050Pakistan Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou UniversityYangzhou 225009P.R.China

Streptococcus agalactiae is one of the most common pathogens that cause bovine mastitis worldwide. Identifying pathogen prevalence and virulence factors is critical for developing prevention and control approaches. Herein, 1 161 milk samples from various dairy farms in China(n=558) and Pakistan(n=603) were collected between 2019–2021 and were subjected to S. agalactiae isolation. Prevalence, serotyping, virulence genes, and antibiotic-resistant genes of S. agalactiae were evaluated by PCR assay. All isolates were characterized for haemolysis, biofilm production, cytotoxicity, adhesion, and invasion on bovine mammary epithelial cells. The prevalence of S. agalactiae-induced mastitis in cattle was found to be considerably higher in Pakistan than in China. Jiangsu and Sindh provinces had the highest area-wise prevalence in China and Pakistan, respectively. Serotypes Ia and II were prevalent in both countries, whereas serotype III was found only in Pakistan. Moreover, all isolates tested positive for PI-2b gene but negative for PI-1 and PI-2a genes. All isolates harboured cfb, cylE, hylB, and fbsB virulent genes, whereas many of them lacked bibA, rib and bca. However, the absence of bac and scp genes in Chinese isolates and cspA in Pakistani isolates was noted, while spb1 and lmb were not detected in isolates of both countries. Pakistani isolates, particularly serotype Iapositive, had a considerably higher ability to produce biofilm, haemolysis, cytotoxicity, adhesion, and invasion than Chinese isolates. Most of the isolates were phenotypically resistant to tetracycline, erythromycin, and clindamycin and genotypic resistance was confirmed by the presence of ermA, ermB, tetM and tetO genes. Our study highlights the antimicrobial resistance profile and virulence-related factors contributing to the epidemiological spread of mastitis-causing S. agalactiae in China and Pakistan. The findings may facilitate future studies designed to develop improved treatment and control str

关键词： Streptococcus agalactiae mastitis epidemiology virulence characterization antibiotic resistance

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Advances in Zika virus vaccines and therapeutics:A systematic review

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Asian Pacific Journal of Tropical Medicine 2024年第3期17卷 97-109页

作者： Shiza Malik Khalid muhammad Omar Ahsan muhammad tahir Khan Ranjit Sah Yasir Waheed Bridging Health Foundation Rawalpindi 46000Pakistan Department of Biology College of SciencesUAE University15551Al AinUnited Arab Emirates Department of Medicine Foundation University Medical CollegeFoundation University IslamabadIslamabad 44000Pakistan INTI International University Persiaran Perdana BBN Putra Nilai71800 NilaiNegeri SembilanMalaysia Institute of Molecular Biology and Biotechnology the University of LahoreKM Defence RoadLahore 58810Pakistan Zhongjing Research and Industrialization Institute of Chinese Medicine Zhongguancun Scientific ParkNanyang 473006China Department of Microbiology Tribhuvan University Teaching HospitalInstitute of MedicineKathmandu 44600Nepal Department of Microbiology Dr.D.Y.Patil Medical CollegeHospital and Research CentreDr.D.Y.Patil VidyapeethPune 411018MaharashtraIndia Department of Public Health Dentistry Dr.D.Y.Patil Dental College and HospitalDr.D.Y.Patil VidyapeethPune 411018MaharashtraIndia Gilbert and Rose-Marie Chagoury School of Medicine Lebanese American UniversityByblos 1401Lebanon

Zika virus(ZIKV)is the causative agent of a viral infection that causes neurological complications in newborns and adults *** wide transmission route and alarming spread rates are of great concern to the scientific *** trials have been conducted to develop treatment options for ZIKV *** review highlights the latest developments in the fields of vaccinology and pharmaceuticals developments for ZIKV infection.A systematic and comprehensive approach was used to gather relevant and up-to-date data so that inferences could be made about the gaps in therapeutic *** results indicate that several therapeutic interventions are being tested against ZIKV infection,such as DNA vaccines,subunit vaccines,live-attenuated vaccines,virus-vector-based vaccines,inactivated vaccines,virus-like particles,and mRNA-based *** addition,approved anti-ZIKV drugs that can reduce the global burden are *** many vaccine candidates for ZIKV are at different stages of development,none of them have received Food and Drug Authority approval for use up to *** issue of side effects associated with these drugs in vulnerable newborns and pregnant women is a major obstacle in the therapeutic pathway.

关键词： Zika virus Infection Therapeutics Antiviral agents Vaccines Therapies Treatment Novel therapeutic Clinical management

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Re-analysis of temperature dependent neutron capture rates and stellarβ-decay rates of^(95-98)Mo

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Chinese Physics C 2024年第9期48卷 142-149页

作者： Abdul Kabir Jameel-Un Nabi muhammad tahir Abdul Muneem Zain Ul Abideen Space and Astrophysics Research lab National Centre of GIS and Space ApplicationsDepartment of Space ScienceInstitute of Space TechnologyIslamabad 44000Pakistan University of Wah Quaid AvenueWah Cantt 47040PunjabPakistan Faculty of Engineering Sciences GIK Institute of Engineering Sciences and TechnologyTopi 23640Khyber PakhtunkhwaPakistan

The neutron capture rates and temperature dependent stellar beta decay rates of Mo isotopes are investigated within the framework of the statistical code TALYS v1.96 and the proton neutron quasi particle random phase approximation(pn-QRPA)*** Maxwellian average cross-section(MACS)and neutron capture rates for the^(95-98)Mo(n,γ)^(96-99)Mo radiative capture process are analyzed within the framework of the statistical code TALYS v1.96 based on the phenomenological nuclear level density model and gamma strength *** present model-based computations for the MACS are comparable to the existing measured *** sensitivity of stellar weak interaction rates to various densities and temperatures is investigated within the framework of the pn-QRPA *** attention is paid to the impact of thermally filled excited states in the decaying nuclei(^(95-98)Mo)on electron emission and positron capture ***,we compare the neutron capture rates and stellar beta decay *** is found that neutron capture rates are higher than stellar beta decay rates at both lower and higher temperatures.

关键词： cross-section TALYS nuclear level density decay rates capture rates

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Room Temperature Synthesis of Vertically Aligned Amorphous Ultrathin NiCo-LDH Nanosheets Bifunctional Flexible Supercapacitor Electrodes

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Energy & Environmental Materials 2024年第2期7卷 277-286页

作者： Kwadwo Asare Owusu Zhaoyang Wang Ali Saad Felix Ofori Boakye muhammad Asim Mushtaq muhammad tahir Ghulam Yasin Dongqing Liu Zhengchun Peng Xingke Cai Institute for Advanced Study Shenzhen UniversityShenzhen 518060China College of Physics and Optoelectronics Shenzhen University3688 Nanhai BoulevardNanshan DistrictShenzhen 518060China School of Chemistry and Materials Science Hubei Engineering UniversityNo.272 Traffic AvenueXiaogan 432000China State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyNo.122 Luoshi RoadWuhan 430070China Key Laboratory of Green Printing CAS Research Centre for Excellence in Molecular ScienceInstitute of Chemistry Chinese Academy of Sciences(ICCAS)Beijing 100190China College of Mechatronics and Control Engineering Shenzhen University3688 Nanhai BoulevardNanshan DistrictShenzhen 518050China

Developing a simple scalable method to fabricate electrodes with high capacity and wide voltage range is desired for the real use of electrochemical ***,we synthesized amorphous NiCo-LDH nanosheets vertically aligned on activated carbon cloth substrate,which was in situ transformed from Co-metal-organic framework materials nano-columns by a simple ion exchange process at room *** to the amorphous and vertically aligned ultrathin structure of NiCo-LDH,the NiCo-LDH/activated carbon cloth composites present high areal capacities of 3770 and 1480 mF cm^(-2)as cathode and anode at 2 mA cm^(-2),and 79.5%and 80%capacity have been preserved at 50 mA cm^(-2).In the meantime,they all showed excellent cycling performance with negligible change after>10000 *** fabricating them into an asymmetric supercapacitor,the device achieves high energy densities(5.61 mWh cm^(-2)and 0.352 mW cm^(-3)).This work provides an innovative strategy for simplifying the design of supercapacitors as well as providing a new understanding of improving the rate capabilities/cycling stability of NiCo-LDH materials.

关键词： amorphous nanosheets aqueous supercapacitor high volumetric/areal energy density NiCo-LDH room temperature synthesis

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Interdigital MnO_(2)/PEDOT Alternating Stacked Microelectrodes for High-Performance On-Chip Microsupercapacitor and Humidity Sensing

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Energy & Environmental Materials 2024年第2期7卷 257-267页

作者： muhammad tahir Lihong Li Liang He Zhongyuan Xiang Zeyu Ma Waqas Ali Haider Xiaoqiao Liao Yanlin Song Key Laboratory of Green Printing CAS Research/Education Centre for Excellence in Molecular SciencesInstitute of Chemistry Chinese Academy of Sciences(ICCAS)Beijing 100190China School of Mechanical Engineering Sichuan UniversityChengdu 610065China A.S.Chemical Laboratories Inc. Concord L4K 4M4ON Canada

For microelectronic devices,the on-chip microsupercapacitors with facile construction and high performance,are attracting researchers'prior consideration due to their high compatibility with modern ***,we proposed interchanging interdigital Au-/MnO_(2)/polyethylene dioxythiophene stacked microsupercapacitor based on a microfabrication process followed by successive electrochemical *** stacked configuration of two pseudocapacitive active microelectrodes meritoriously leads to an enhanced contact area between MnO_(2)and the conductive and electroactive layer of polyethylene dioxythiophene,hence providing excellent electron transport and diffusion pathways of electrolyte ions,resulting in increased pseudocapacitance of MnO_(2)and polyethylene *** stacked quasi-solid-state microsupercapacitors delivered the maximum specific capacitance of 43 mF cm^(-2)(211.9 F cm^(-3)),an energy density of 3.8μWh cm^(-2)(at a voltage window of 0.8 V)and 5.1μWh cm^(-2)(at a voltage window of 1.0 V)with excellent rate capability(96.6%at 2 mA cm^(-2))and cycling performance of 85.3%retention of initial capacitance after 10000 consecutive cycles at a current density of 5 mA cm^(-2),higher than those of ever reported polyethylene dioxythiophene and MnO_(2)-based planar *** from the favorable morphology,bilayer microsupercapacitor is utilized as a flexible humidity sensor with a response/relaxation time superior to those of some commercially available integrated *** strategy will be of significance in developing high-performance on-chip integrated microsupercapacitors/microsensors at low cost and environment-friendly routes.

关键词： electrochemical polymerization microsensor microsupercapacitor stacked microelectrode

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Variable range hopping transport and dielectric relaxation mechanism in GdCrO_(3) rare-earth orthochromite perovskite

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Journal of Rare Earths 2024年第7期42卷 1304-1316,I0004页

作者： muhammad Javed Ayaz Arif Khan Jamal Kazmi Naeem Akbar Said Nasir Khisro Amanullah Dar aleem Dad Khan Tareen Mohd Ambri Mohamed Department of Physics The University of Azad Jammu and KashmirMuzaffarabad13100Pakistan Department of Physics University of Kotli Azad Jammu and KashmirKotli11100Pakistan Institute of Microengineering and Nanoelectronics Universiti Kebangsaan Malaysia43600BangiSelangorMalaysia Department of Mathematics University of Kotli Azad Jammu and KashmirKotli11100Pakistan

The polycrystalline GdCrO_(3)(GCO)perovskite orthochromite was synthesized by the sol-gel auto-combustion *** Rietveld refined X-ray diffraction(XRD)profile of GCO confirms that the orthorhombic phase crystallizes in perovskite crystal structure with space group *** transmission electron microscopy and selected area electron diffraction divulge the polycrystalline nature of the perovskite *** field emission scanning electron microscopy(FESEM)images reveal the microstructural polyhedral growth of grains separated by sharp grain *** chemical states of the ions present in the compound were investigated by X-ray photoelectron spectroscopy(XPS)studies,and the electrical pathways were *** impedance studies were carried out in the experimental frequency and temperature ranges of 40 Hz-4 MHz and 178-408 K,*** equivalent circuit model(RGQG)(RGBQGB)resolves the impedance response into two contributions originating from the intra-grain and inter-granular micro-constituents of the *** electrical conduction mechanism is based on the Mott variable range hopping *** complex electric modulus studies demonstrate the short-and long-range mobility of the charge carriers and rule out the presence of electrode effects on the dielectric relaxation mechanism of the *** decreasing pattern of dielectric permittivity with frequency is explained by the Maxwell-Wagner type of *** frequency dependence of the loss factor exhibits the presence of thermally activated relaxation time distribution in the material.

关键词： Auto-combustion Perovskite Electro-ceramic Dielectric Electrical transport Impedance

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Standardization of Famacha? system for natural parasites infected goat by comparing mucosa color

Standardization of Famacha? system for natural parasites inf...

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中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论坛

作者： Mubashar Hassan Zafar Iqbal Zia ud Din Sindhu Awais Ali Ahmad Aftab Shaukat Shahid Ali Rajput muhammad tahir aleem muhammad Mohsin State Key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural University Deparment of Veterinary Parasitology University of Agriculture Faisalabad Department of Clinical Veterinary Medicine College of Veterinary MedicineHuazhong Agricultural University Department of Animal Nutrition and Feed Science College of Animal Science and TechnologyHuazhong Agricultural University College of veterinary science Preventive Veterinary MedicineNanjing Agriculture University College of Life Sciences Fujian Agriculture and Forestry University

Haemonchus contortus is the most significant blood sucking abomasal *** has been playing a vital role to control ***,ability of *** to develop resistance against anthelmintics has led to scientists to control this rapid perpetual rate FAMACHA0 system has been introduced for selective and targeted use of the *** system works on scoring of the eye mucosal color,red to pale,with increasing anemic condition of the *** this study,sensitivity of FAMACHA was evaluated by faecal egg counts(FEC),packed cell volume(PCV) and haemoglobin(Hb) concentration in three breeds of goat ***,Pak Angora and *** Nachi and Pak Angora goats FEC values were concordant with FAMCAHA score but PCV and Hb values were in negative *** Beetal goats,FEC and PCV values were concordant to FAMACHA but Hb concentration was in negative *** conclusion,FAMCAHA system is not found competent in selective and targeted treatment system.

关键词： FAMACHA Haemonchus contortus goats color meter Diagnosis

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Actin-depolymerizing factor from Eimeria tenella promotes immunogenic function of chicken dendritic cells

Actin-depolymerizing factor from Eimeria tenella promotes im...

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2021中国畜牧兽医学会兽医寄生虫学分会第二届青年科学家学术论坛

作者： Shakeel Ahmed Lakho muhammad Haseeb Jianmei Huang Zhang Yang muhammad Waqqas Hasan muhammad tahir aleem muhammad Ali Memon XiaoKai Song RuoFeng Yan Lixin Xu 李祥瑞 College of Veterinary Medicine Nanjing Agricultural University

Dendritic cells play a crucial role in inducing antigen-specific immunity to *** host-parasite interaction,host immune response to the parasite molecules is considered essential for recognizing novel antigens for control ***,in the present study,chicken dendritic cells(DCs)(ChDCs),derived from spleens were used to evaluate their capacity to proliferate and differentiate autologous T lymphocytes in response to actin-depolymerizing factor from Eimeria tenella(EtADF).Immunoblot analysis showed that recombinant EtADF protein(rEtADF) was able to interact with rat anti-rEtADF *** immunofluorescence test confirmed rEtADF binding on ChDCs *** *** revealed that phenotypes for MHCII,CD1.1,CD11 c,CD80,and CD86 were increased in ChDCs after rEtADF ***-PCR results indicated that ChDCs triggered TLR signaling in response to rEtADF,and suppressed Wnt *** levels of CD83,CCL5,and CCR7 in ChDCs were improved following rEtADF *** addition,rEtADF promoted DC-directed T cell proliferation and differentiation of naive T cells into CD3/CD4T cells in DC/T cell co-incubation *** analysis of rEtADF-pulsed ChDCs showed increased levels of IL-12 and IFN-γ,while IL-10 and TGF-β remained ***,rEtADF-treated ChDCs enhanced production of IFN-γ when incubated with T cells,and IL-4 secretion remained *** findings indicted that rEtADF could facilitate the polarization of Th1 immune cells by triggering both host DCs and T *** findings provide useful insights into future work aimed at anticoccidial vaccine strategies.

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Immunogenicity and Protective Efficacy of Probiotics with EtIMP1C against Eimeria tenella Challenge

Immunogenicity and Protective Efficacy of Probiotics with Et...

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2021中国畜牧兽医学会兽医寄生虫学分会第二届青年科学家学术论坛

作者： muhammad Mohsin Lina Li Xiaohang Huang muhammad tahir aleem Yusuf Jibril Habib Akram Ismael Shehata muhammad Zeeshan Afzal Rao Zahid Abbas Asghar Abbas Guangwen Yin College of Animal Sciences (College of Bee Science) Fujian Agriculture and Forestry University College of Life Science Fujian Agriculture and Forestry University Department of Parasitology University of Agriculture MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary MedicineNanjing Agricultural University Department of Pathology University of Agriculture

Coccidiosis is an endemic problem in broilers inflicting disastrous losses to worldwide poultry *** are generally effective in controlling the infectious diseases;the subunit vaccines effectiveness can hopefully be improved with concurrent use of probiotics as assessed in this experiment using an Eimeria tenella *** mapped protein-1(IMP 1)is a novel immunogenic protein of Apicomplexans,including in Eimeria *** performance of the cumulative effect of probiotics and EtIMP1 C(Part of Eimeria tenella immune mapped protein1) was evaluated based on various parameters such as an intestinal lesion,oocyst scores,feed conversion ratio,and organ *** were also analyzed on both immunological and hematological *** EtIMP1 C and probiotics administered group showed less intestinal lesion,decreased oocyst shedding,satisfactory feed conversion ratio(FCR) and improved hematological parameters as compared to EtIMP1 C emulsified with FCA ***,there was no statistically significant difference between the two groups(P>0.05),in aspect of lesion and oocyst scores,as well as immunological and hematological *** experimental work showed that probiotics could be a good hope due to its antioxidant,immunomodulatory and growth promoting effect against poultry coccidiosis alone or in combination with vaccines,including IMP1 C based vaccine,but further studies are required to formulate its dose with the vaccine as well as a different strain of probiotics effect against coccidiosis.

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Glyceraldehyde-3-phosphate dehydrogenase from Eimeria acervulina modulates the functions of chicken dendritic cells to boost Th1 type immune response and stimulates autologous CD4+T cells differentiation in-vitro

Glyceraldehyde-3-phosphate dehydrogenase from Eimeria acervu...

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2021中国畜牧兽医学会兽医寄生虫学分会第二届青年科学家学术论坛

作者： Shakeel Ahmed Lakho muhammad Haseeb Jianmei Huang Zhang Yang muhammad Waqqas Hasan muhammad tahir aleem muhammad Ali-Ul-Husnain Naqvi muhammad Ali Memon XiaoKai Song RuoFeng Yan Lixin Xu 李祥瑞 College of Veterinary Medicine Nanjing Agricultural University

Dendritic cells(DCs) play a pivotal role to amplify antigen-specific immune *** that sensitize T cells via antigen-presentation by DCs could enhance the capacity of host immunity to fight *** this study,we tested the immunogenic profiles of chicken DCs towards Glyceraldehyde-3-phosphate dehydrogenase from Eimeria acervulina(EaGAPDH).Immunoblot analysis showed that recombinant EaGAPDH(rEaGAPDH) protein was successfully recognized by rat sera generated against *** and internalisation of rEaGAPDH by chicken splenic-derived DCs(chSPDCs) was confirmed by immunofluorescence *** cytometry revealed that chSPDCs upregulated MHCII,CD 1.1,CD11 c,CD80,and CD86 cell-surface ***,mRNA expressions of DC maturation biomarkers(CCL5,CCR7,and CD83) and TLR signalling genes(TLR15 and MyD88) were also upregulated whereas those of Wnt signalling were non-significant compared to negative *** treatment induced IL-12 and IFN-γ secretion in chSPDCs but had no effect on IL-10 and TGF-β.Likewise,DC-T cell co-culture promoted IFN-γ secretion and the level of IL-4 was *** of T cells and their differentiation into CD3+/CD4+T cells were triggered in chSPDCs-T cells co-culture *** together,rEaGAPDH could promote Th1 polarization by activating both host DCs and T cells and sheds new light on the role of this important molecule which might contribute to the development of new DCs-based immunotherapeutic strategies against coccidiosis.

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