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Evaluation of antileishmanial,antibacterial and brine shrimp cytotoxic potential of crude methanolic extract of Herb Ocimum basilicum(Lamiacea)

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Journal of Traditional Chinese Medicine 2015年第3期35卷 316-322页

作者： Imran Khan Kafeel Ahmad Ali Talha Khalil Jangrez Khan Yusra Ali Khan muhammad shahab saqib muhammad Naveed Umar Hilal Ahmad Department of Biotechnology Quaid-i-Azam University Centre of Biotechnology and Microbiology University of Peshawar Department of Biochemistry Quaid-i-Azam University Islamabad Department of Chemistry University of Malakand Institute of Biotechnology and Genetic Engineering Agriculture UniversityPeshawar

OBJECTIVE:To collect and screen for ethnopharmacological properties(antileishmanial,antibacterial and brine lethality assays) of medicinal plant Ocimum basilicum from Peshawar region(34.008 latitude and 71.57 altitudes).METHODS:In the present study a general antileishmanial activity against Leishmania tropica strain was carried *** antibacterial potential of the plant was performed against 06 gram positive and 06 gram negative *** shrimp cytotoxicity assay at different concentrations were ***:The anti-promastigotes profile of the plant showed good antileishmanial activity exhibited LC_(50) value 21.67 μg/*** result for gram positive antibacterial activity revealed that the *** leaves extract possesses significant inhibitory activity at highest two concentrations ranging from20.66 ± 0.31 to 31.86 ± 0.80 for Clostridium perfringens type C and Bacillus subtitilis,respectively,as compared to the gentamycin(27.36 ± 0.55 and21.80 ± 0.72,respectively).For gram negative bacteria good activity was observed.A highest zone of inhibition was recorded for Pseudomonas aeroginosa(28.83 ± 0.28) atthe highest concentration(10 mg/mL).The LC_(50) value obtained for brine shrimp lethality assay was 91.56 μg/***:The herb basil possesses effective cidal activities which make this plant a good candidate for the isolation of antiprotozoal and antibacterial compounds which may lead to the development of novel drug.

关键词： Ocimum basilicum Cytotoxicity tests,immunologic Anti-bacterial agents Antileishmanial activity

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Care and social support from family and community in patients with pulmonary tuberculosis in Pakistan

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Family Medicine and Community Health 2019年第4期7卷 24-32页

作者： shahab E saqib Mokbul Morshed Ahmad Sanaullah Panezai Department of Regional and Rural Development Planning Asian Institute of TechnologyBangkokThailand Department of Geography University of BalochistanQuettaPakistan

Objectives This study aimed to validate the Medical Outcome Study-Social Support Survey(MOS-SSS)instrument in Pakistan and to quantify the nature of care and social support currently extended to patients with pulmonary tuberculosis(TB)in *** This is a cross-sectional study design conducted within a period of 3 months from 1 November 2016 to 31 January *** A total of 269 patients,registered at 11 TB centres and private clinics,were interviewed through an interviewer-administered *** outcome measures Cronbach’s alpha was used to measure the internal consistency and reliability of the MOS-SSS survey *** and multivariable logistic regressions have been used to explore the association between care and social support,and socioeconomic *** This study validated the use of the MOS-SSS among patients with TB in *** of the study revealed significant differences among the socioeconomic groups of patients in each subscale of social ***,results of logistic regressions showed that patients who were older(adjusted OR=6.17,95%CI 1.55 to 24.59,p≤0.01),male(adjusted OR=2.73,95%CI 1.49 to 4.98,p≤0.01),widow(adjusted OR=0.17,95%CI 0.04 to 0.80,p≤0.05),and had a larger household size(adjusted OR=5.69,95%CI 1.32 to 24.65,p≤0.05),higher monthly income(adjusted OR=2.00,95%CI 1.11 to 3.60,p≤0.05)and house ownership(adjusted OR=1.99,95%CI 1.10 to 3.60,p≤0.05)were significant factors associated with the extent of care and social support that the participants *** To cure TB,this study suggests a coordinated approach that includes not only clinical services to address this issue but also a strong social support system based on family and community necessary throughout the treatment process.

关键词： patients tuberculosis instrument

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