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Plantlets Regeneration via Somatic Embryogenesis from the Nucellus Tissues of Kinnow Mandarin (Citrus reticulata L.)

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American Journal of Plant Sciences 2016年第6期7卷 798-805页

作者： Mubashir Hussain Naveed Iqbal Raja muhammad Iqbal Anam Iftikhar Huma Mehreen Sadaf Sidra Sabir muhammad Asim Sultan muhammad nasim ashraf faz Department of Botany PMAS Arid Agriculture University Rawalpindi Pakistan Department of Botany University of Sargodha Sargodha Pakistan Department of Botany Government College University Faisalabad Pakistan

Studies were initiated to explore the role of nucellus tissues and growth regulators in plantlets regeneration via somatic embryogenesis of Kinnow mandarin [Citrus reticulata L. (Blanco)]. Nucellus tissues were cultured on MS medium supplemented with different concentrations and combinations of auxins, cytokinins and malt extract for primary callus induction. The best response for primary callus induction (90%) was obtained when MS medium was supplemented with 5 mg/l 2,4-D and 500 mg/l malt extract. Best results for embryogenic callus induction (80%) were obtained in C8 medium. The induction of somatic embryos was highest when MS medium was supplemented with 1 mg/l BAP and maturation of somatic embryos occurred when MS medium was supplemented with 5 mg/l 2,4-D and 1 mg/l BAP. Maximum plantlets were regenerated (92%) from the somatic embryos on half strength MS medium with no hormones. The plantlets were successfully acclimatized in different potting mixtures and highest survival rate (100%) was achieved in potting mixture containing sand and peat moss (2:1).

关键词： Tissue Culture MS Media Citrus Nucellus Tissue Callogenesis Somatic Embryos

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Effect of Plant Derived Smoke on Germination and Post Germination Expression of Wheat (Triticum aestivum L.)

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American Journal of Plant Sciences 2016年第6期7卷 806-813页

作者： muhammad Iqbal Saira Asif Noshin Ilyas Naveed Iqbal Raja Mubashir Hussain Sumera Shabir muhammad nasim ashraf faz Abdul Rauf Department of Botany PMAS-Arid Agriculture University Rawalpindi Pakistan Department of Botany Government College University Faisalabad Pakistan Department of Biology AIOU Islamabad Pakistan

Smoke produced from burning of plant material elicits a striking increase in seed germination in various plant species. In present work, effect of plant derived smoke exposure on wheat (Triticum aestivum L.) seeds germination and post germination response has been studied in laboratory. Plant derived smoke was generated by burning of plant material (leaf, straws etc.) in a special designed furnace. Non-imbibed and imbibed seeds of wheat were exposed to plant derived smoke for 1 hr time duration. Best results were observed in non-imbibed seeds treated with plant derived smoke while imbibed seeds showed poor response to germination percentage, germination index, seedling vigor index and root shoot length. It has been proved from present study that plant derived smoke has stimulatory effect on germination & post germination response of non-imbibed seeds while it may cause inhibitory effect in imbibed seeds of wheat.

关键词： Plant Derived Smoke Wheat Seed Germination Seedling Vigor Root Shoot Length

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