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Genetic Variations and Cultivar Purity within the G. <i>hirsutum</i>Cultivars via RAPD Marker

American Journal of Plant Sciences 2015年第2期6卷 392-404页

作者： Farah Raheel muhammad kauser nawaz shah muhammad shahid Iqbal Ijaz Rasool Noorka Zareen Sarfraz Government Postgraduate College (w) Multan Pakistan PMAS Arid Agricultural University Rawalpindi Pakistan Ayub Agriculture Research Institute Faisalabad Pakistan Department of Plant Breeding and Genetics University College of Agriculture University of Sargodha Sargodha Pakistan National Institute of Biotechnology and Genetic Engineering Faisalabad Pakistan

To assess genetic variation within a cultivar, four commercial varieties with five individual G. hirsutum genotypes and their bulk were assayed with selected forty 10-mer RAPDs per cultivar via PCR. CIM-473 cultivar showed amplification of 321 bands in total per primer with an average of 8 bands. A range of similarity i.e.;80% - 100% within the cultivar has been observed for minimum and maximum respectively. CIM-448 showed amplification of 300 bands in total per primer with an average of 7.5 bands. Maximum similarity of 95% and minimum similarity of 84% was observed within the cultivar. In CIM-1100 the total numbers of fragments amplified are 304, with the average of 7.6 bands per primer and are 12% - 20% polymorphic. Similarly, in NIAB-78 the total numbers of fragments amplified were 310, with the average of 7.7 bands per primer. The reliability of RAPD technique for finding genetic resemblances is indicated by the mentioned results. Numerous groups within 5 individual genotypes bulk among 4 cultivars of G. hirsutum L. have been through cluster analysis which was by the unweighted pair group method of arithmetic means (UPGMA). Dendrograms have been used for showing cultivar relationship.

关键词： Polymerase Chain Reaction (PCR) G. hirsutum RAPD Variation within the Cultivars

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