检索结果-南通市图书馆

中国畜牧兽医 2024年第9期51卷 3752-3761页

作者：李晋达彭自伟 muhammad ameen jamal 角德灵魏红江云南省小型猪基因编辑与异种器官移植重点实验室昆明650201 云南农业大学动物医学院昆明650201 云南农业大学动物科学技术学院昆明650201

【目的】建立不同年龄、不同组织来源的亚洲象成纤维细胞系,并进行冷冻保存和细胞生物学特性研究,为实现濒危动物遗传资源保存和开发利用提供理论依据。【方法】分别采集6岁龄亚洲象耳缘组织(AE00)、新生亚洲象脐带组织(AE01)及死亡16 ... 详细信息

【目的】建立不同年龄、不同组织来源的亚洲象成纤维细胞系,并进行冷冻保存和细胞生物学特性研究,为实现濒危动物遗传资源保存和开发利用提供理论依据。【方法】分别采集6岁龄亚洲象耳缘组织(AE00)、新生亚洲象脐带组织(AE01)及死亡16 h后的4岁龄亚洲象耳缘组织(AE02)样品,分别建立成纤维细胞系并进行传代培养和冷冻保存,通过细胞形态学、增殖能力、衰老及凋亡水平分析不同年龄、不同组织来源成纤维细胞的生物学特性,并进行绿色荧光蛋白(GFP)转染筛选和核型检测,分析亚洲象成纤维细胞的遗传稳定性。【结果】成功建立了3头亚洲象成纤维细胞系并进行冷冻保存,复苏后细胞均能保持一定水平的活力,经连续传代培养后,细胞形态均呈梭形;AE02成纤维细胞冻存活力、细胞增殖能力均最佳,细胞凋亡率依次为AE01(16.7%)>AE00(6.4%)>AE02(3.8%),细胞衰老率依次为AE00(35.9%)>AE01(17.2%)>AE02(7.8%);AE02细胞经长达19次传代后,细胞染色体数目稳定,AE00进行GFP转染筛选可获得稳定表达GFP的亚洲象成纤维细胞系。【结论】本研究成功建立了不同年龄、不同组织来源的亚洲象成纤维细胞系,细胞形态均为梭形,不同年龄、不同组织来源的成纤维细胞系增殖能力、细胞衰老及凋亡水平均不同,经连续传代后细胞染色体数目稳定,能实现外源基因的稳定转染和长期筛选,可作为濒危动物遗传资源长期保存和复活个体的重要方法之一。

关键词：亚洲象成纤维细胞生物学特性

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

First report on molecular characterization of Leishmania species from cutaneous leishmaniasis patients in southern Khyber Pakhtunkhwa province of Pakistan

引用

Asian Pacific Journal of Tropical Medicine 2017年第7期10卷 785-788页

作者： Mubbashir Hussain Shahzad Munir Sultan Ayaz Bahar Ullah Khattak Taj Ali Khan Niaz muhammad muhammad Anees Hazir Rahman muhammad Qasim muhammad ameen jamal Irfan Ahmed Kashif Rahim Humaira Mazhar Noha Watanay Mohamed Kasbari Vector Borne Diseases Laboratory Department of MicrobiologyKohat University of Science and Technology Kohat Department of Plant Pathology Faculty of Plant ProtectionYunnan Agricultural University College of Animal Husbandry and Veterinary Sciences Abdul Wali Khan University Mardan College of Animal Science and Technology Yunnan Agricultural University Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Yunnan Agricultural University Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology Institute of Biochemistry and BiotechnologyCollege of Life SciencesBeijing Normal University US Naval Medical Research Unit-3 (NAMRU 3) ANSES Animal Health Laboratory

Objective: To report presence of Leishmania major in Khyber Pakhtunkhwa of Pakistan, where cutaneous leishmaniasis(CL) is endemic and was thought to be caused by Leishmania tropica only. Methods: Biopsy samples from 432 CL suspected patients were collected from 3 southern districts of Khyber Pakhtunkhwa during years 2011–2016. Microscopy on Giemsa stained slides were done followed by amplification of the ribosomal internal transcribed spacer 1 gene. Results: Leishmania amastigotes were detected by microscopy in 308 of 432 samples(71.3%) while 374 out of 432 samples(86.6%) were positive by ribosomal internal transcribed spacer 1 PCR. Subsequent restriction fragment length polymorphism confirmed Leishmania tropica in 351 and Leishmania major in 6 biopsy samples. Conclusions: This study is the first molecular characterization of Leishmania species in southern Khyber Pakhtunkhwa. It confirmed the previous assumptions that anthroponotic CL is the major CL form present in Khyber Pakhtunkhwa province. Furthermore, this is the first report of Leishmania major from a classical anthroponotic CL endemic focus identified in rural areas of Kohat district in southern Khyber Pakhtunkhwa.

关键词： Cutaneous leishmaniasis ITS-PCR RFLP Leishmania tropica Khyber Pakhtunkhwa

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular detection of Leishmania species in human and animals from cutaneous leishmaniasis endemic areas of Waziristan, Khyber Pakhtunkhwa, Pakistan

引用

Asian Pacific Journal of Tropical Medicine 2018年第8期11卷 495-500页

作者： Mubashir Hussain Shahzad Munir Abdullah Jalal Taj Ali Khan Niaz muhammad Bahar Ullah Khattak Abdullah Khan Irfan Ahmed Zulqarnain Baloch Nawaz Haider Bashir muhammad ameen jamal Kashif Rahim Humaira Mazhar Maira Riaz Noha Watany Vector Borne Diseases Lab Department of Microbiology Kohat University of Science and Technology Kohat Faculty of Plant Protection Yunnan Agricultural University Institute of Biotechnology and Genetic Engineering The University of AgriculturePeshawar Pakistan Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Yunnan Agricultural University College of Veterinary Medicine South China Agricultural University Department of Animal Breeding Genetics and Reproduction Yunnan Agricultural University Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology Institute of Biochemistry and Biotechnology College of Life Sciences Beijing Normal University Vector Biology Research Lab US Naval Medical Research Unit 3

Objectives: To detect Leishmania species in human patients, animal reservoirs and Phlebotomus sandflies in Waziristan, Pakistan. Methods: Tissue smears and aspirates from 448 cutaneous leishmaniasis(CL) suspected patients were analyzed. To sort out role of the reservoir hosts, skin scrapings, spleen and liver samples from 104 rodents were collected. Furthermore, buffy coat samples were obtained from 60 domestic animals. Sandflies were also trapped. All human, animals and sandfly samples were tested by microscopy, kinetoplastic PCR and internal transcribed spacer 1(ITS1) PCR followed by restriction fragment length polymorphism for detection of Leishmania species. Results: An overall prevalence of 3.83% and 5.21% through microscopy and ITS1 PCR respectively was found. However, the statistically non-significant correlation was found between area, gender, and number of lesions. The presence of rodents, sandflies, domestic animals and internally displaced people increased the risk of CL. Using ITS1-PCR-RFLP, Leishmania tropica(L. tropica) was confirmed in 106 samples while 25 of the isolates were diagnosed as Leishmania major(L. major). Similarly, 3/104 rodents were positive for L. major and 14 pools of DNA samples containing Phlebotomus sergenti sandflies were positive for L. tropica. None of samples from domestic animals were positive for leishmaniasis. Conclusions: In the present study, L. tropica and L. major are found to be the main causative agents of CL in study area. Movement of internally displaced people from CL endemic areas presents a risk for nearby CL free areas. To the best of our knowledge, we report for the first time L. major infection in rodents(Rattus rattus) and L. tropica in Phlebotomus sergenti sandflies trapped in Waziristan, Pakistan.

关键词： Leishmania major Rodents Cutaneous leishmaniasis Domestic animals ITS 1 PCR Waziristan

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

In vitro and in vivo development of interspecies Asian elephant embryos reconstructed with pig enucleated oocytes

In vitro and in vivo development of interspecies Asian eleph...

引用

中国畜牧兽医学会动物繁殖学分会第二十次学术研讨会

作者： Tien Dat Nguyen Honghui Li Yiquan Zhuang Bowei Chen Keiji Kinoshita muhammad ameen jamal Kaixiang Xu Jianxiong Guo Deling Jiao Kumiko Tanabe Yunfang Wei Zhuo Li Wenming Cheng Yubo Qing Hong-Ye Zhao Hong-Jiang Wei

Purpose De-extinction to bring back the wooly mammoths would be a tremendous accomplishment for humanity and has always been the dream of *** revive them,three main strategies （ICSI,SCNT,gene-editing） using elephant oocytes are under *** the elephant oocytes seem to be an indispensable recipient for reviving the wooly ***,the elephant （Elephas maximus.L） is also categorized as an endangered *** somatic cell nuclear transfer（iSCNT） has an immense potential to rescue endangered animals and extinct species like mammoths.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

High-Bandwidth,Low-Power CMOS Transistor Based CAB for Field Programmable Analog Array

引用

Computers, Materials & Continua 2023年第3期74卷 5885-5900页

作者： ameen Bin Obadi Alaa El-Din Hussein Samir Salem Al-Bawri Kabir Hossain Abdullah Abdulhameed Muzammil Jusoh Thennarasan Sabapathy Ahmed jamal Abdullah Al-Gburi Mahmoud A.Albreem Advanced Communication Engineering(ACE) Centre of ExcellenceUniversiti Malaysia Perlis(UniMAP)Faculty of Electronic Engineering TechnologyKangar01000Malaysia Electrical Engineering King Fahd University of Petroleum and MineralsDhahranEastern31261Saudi Arabia Space Science Centre Climate Change InstituteUniversiti Kebangsaan MalaysiaBangi43600Malaysia Department of Electronics&Communication Engineering Faculty of Engineering&PetroleumHadhramout UniversityAl-MukallaHadhramout50512Yemen Faculty of Engineering Norwegian University of Science and Technology(NTNU)GjovikN-2815Norway Faculty of Electronic Engineering Technology Universiti Malaysia Perlis(UniMAP)Kampus Alam UniMAP Pauh PutraArau02600Malaysia Department of General Educational Development Faculty of Science and Information Technology(FSIT)Daffodil International UniversityDhaka1207Bangladesh Department of Electronics and Computer Engineering(FKEKK) Center for Telecommunication Research and Innovation(CeTRI)Universiti Teknikal Malaysia Melaka(UTeM)Durian Tungal76100Malaysia Department of Electrical Engineering University of SharjahSharjah27272United Arab Emirates

This article presents an integrated current mode configurable analog block(CAB)system for field-programmable analog array(FPAA).The proposed architecture is based on the complementary metal-oxide semiconductor(CMOS)transistor level design where MOSFET transistors operating in the saturation region are *** proposed CAB architecture is designed to implement six of thewidely used current mode operations in analog processing systems:addition,subtraction,integration,multiplication,division,and pass *** functionality of the proposed CAB is demonstrated through these six operations,where each operation is chosen based on the user’s selection in the CAB interface *** architecture of the CAB system proposes an optimized way of designing and integrating only three functional cells with the interface circuitry to achieve the six ***,optimized programming and digital tuning circuitry are implemented in the architecture to control and interface with the functional ***,these designed programming and tuning circuitries play an essential role in optimizing the performance of the proposed *** of the proposed CMOS Transistor Based CAB system is carried out using Tanner EDA Tools in 0.35μm standard CMOS *** design uses a±1.5 V power supply and results in maximum 3 dB bandwidth of 34.9 MHz and an approximate size of 0.0537 *** demonstrates the advantages of the design over the current state-of-the-art designs presented for comparison in this ***,the proposed design has a clear aspect of simplicity,low power consumption,and high bandwidth operation,which makes it a suitable candidate for mobile telecommunications applications.

关键词： CMOS field programmable analog array configurable analog block current mode circuit

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Optimizing the Global Digital Elevation Models(GDEMs) and accuracy of derived DEMs from GPS points for Iraq’s mountainous areas

引用

Geodesy and Geodynamics 2020年第5期11卷 338-349页

作者： Shazad jamal Jalal Tajul Ariffin Musa Taher Hama ameen Ami Hassan Md Din Wan Anom Wan Aris Jwan Mohammad Ebrahim Faculty of Built Environment and Surveying Universiti Teknologi Malaysia Johor Bahru 81310 Malaysia College of Engineering University of Sulaimani 46001 Sulaimani Iraq University of Arkansas at Little Rock Little Rock 72204 AR USA SALP Middle East Ltd. Company for Oil and Gas Pipeline Construction Erbil Iraq

Optimizing the combined horizontal and vertical accuracy of the well-known Global Digital Elevation Models(GDEMs) of various resolutions for each country and region especially in Iraq’s mountainous areas is still questionable. All the three GDEMs, approximately, have the same vertical accuracy with the Root Mean Square(RMSE) of ±7.3 m, ±7.6 m and ±6.5 m via 12 fixed Ground Control Points(GCPs) for the Advanced Land Observation Satellite Phased Array L-band Synthetic Aperture Radar(ALOS PALSAR 12.5 m), the Shuttle Radar Topographic Mission(SRTM 30 m) and the TerraSAR-X(the name of twin satellites) add-on for Digital Elevation Measurement(TanDEM-X 90 m) GDEMs respectively. Moreover, the percentage of outliers that are greater or smaller than ±10 m detection of the height extraction from both the ALOS PALSAR and SRTM Digital Elevation Models(DEMs) contains 16.7% and for TanDEM-X was 25%. In this paper,the special DEM is derived using 2123 handheld GPS points for Sulaymaniyah Governorate, Kurdistan region, Iraq. The height extraction by discarding the outliers of 58% gives the RMSE of ±8.0 m in the case of adding geoid heights(N) to the ellipsoidal heights(h) via the Earth Gravitational Model 2008(EGM2008)and ±5.6 m without adding N. It is expected that the derived DEMs will give more accurate results both horizontally and vertically in the mountainous areas when GPS observations are intensified. The horizontal accuracy is validated through extracting hierarchy stream types of the watershed map from the DEMs for higher than 100 pixels length. The ALOS PALSAR DEM extracted more numbers of stream orders than ***, based on the criteria of RMSE, outlier detection, and the number of extracted stream orders, the ALOS PALSER DEM is regarded as the optimal GDEM in comparison with the close accuracy of both the TanDEM-X and SRTM DEM.

关键词： EGM2008 PALSAR SRTM

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：