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Evolutionary history and phylogeography of Scots pine (Pinus sylvestris L.) in Europe based on molecular markers

Journal of Forestry Research 2017年第4期28卷 637-651页

作者： Endre Gy. To′th Zolta′n A. Kobolkuti Andrzej Pedryc ma′ria.Hohn Department of Botany Szent Istva′n University Me′nesi u′t 44Budapest 1118 Hungary Department of Genetics and Plant Breeding Szent Istva′n UniversityMe′nesi u′t 44Budapest 1118Hungary

In this review we summarized recent historical records and molecular studies on evolutionary history and phylogeography of Scots pine with focus on the European highly fragmented distribution area of the species. Fossilized pollen, plant micro- and macrofossil records provided evidences on the large-scale species’ range shifts and demographic changes during the Quaternary. Populations of Scots pine were documented both in the glacial (incl. full glaciation) and interglacial periods. Recolonization of Europe after the glaciation originated from the (Sub) Mediterranean areas like the Balkan Peninsula but also from around the Eastern Alps and the surroundings of the Danube plain. Fennoscandia and northern European Baltic regions were most probably colonized from two main directions, from Western Europe and from the Russian Plain. Modern history of Scots pine was hardly affected by anthropogenic activities that started to strengthen in the Bronze and Iron Age. Along with the fossil records, molecular genetic tools were used to infer the origin and putative history including migration, differentiation and demography of the species. In this paper we compiled the major publications (30) of molecular genetic studies of the past 20 years derived from distinctly inherited organelle genomes (mitochondria., chloroplast, nuclear) revealed by different marker systems (mtDNA-cox1, -nad1, -nad3, -nad7, ISSR, cpSSR, nSSR, B-SAP, SNP). It is important to consider that different phylogeographic patterns can be drawn by the analysis of different DNA marker types. Accordingly the use of more than one marker simultaneously outlines the most sophisticated phylogeographical pattern on the genetic lineages and can reveal high differentiation of the European distribution. Combined marker systems and markers derived from coding sequences have also been used to detect species’ phylogeographic patterns, but these were rarely applied to Scots pine. Although new molecular techniques can provide h

关键词： Molecular genetic markers Phylogeography Pinus sylvestris Quaternary history Refugia

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脑性瘫痪儿童采用头部低频电刺激对其运动功能及脑血流的影响

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中国现代医生 2012年第8期50卷 54-55,58页

作者：玛依努尔.吐尔逊沙比拉.沙比提乌鲁木齐市儿童医院小儿康复医学科新疆乌鲁木齐830000 乌鲁木齐市第一人民医院儿科新疆乌鲁木齐830000

目的探讨对脑性瘫痪(脑瘫)儿童给予双乳突法头部低频电刺激对其运动功能及脑血流的影响。方法收集来我院进行治疗的脑瘫患儿106例,按照随机数字表法进行分组,康复对照组53例,给予常规治疗,电刺激治疗组53例,给予常规治疗+双乳突法头部... 详细信息

目的探讨对脑性瘫痪(脑瘫)儿童给予双乳突法头部低频电刺激对其运动功能及脑血流的影响。方法收集来我院进行治疗的脑瘫患儿106例,按照随机数字表法进行分组,康复对照组53例,给予常规治疗,电刺激治疗组53例,给予常规治疗+双乳突法头部低频电刺激;正常对照组为同期来我院进行健康体检的健康儿童50例。结果与康复对照组治疗后比较,电刺激治疗组患者的治疗显效率及其有效率明显较高(P<0.05),电刺激治疗组患儿具有显著较高的GMFM评分(P<0.05)。与电刺激治疗组治疗前比较,治疗后ACA、MCA及PCA的平均血流速度均显著增加(P<0.05)。结论对脑性瘫痪(脑瘫)儿童给予双乳突法头部低频电刺激可以使其运动功能得到明显的改善,疗效得到显著增强。

关键词：低频电刺激脑性瘫痪运动功能脑血流

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Agriculture and Forest Transition: Understanding of Land Use Change in a Cultural Landscape

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Open Journal of Applied Sciences 2015年第12期5卷 797-807页

作者： José Eduardo dos Santos Angela Terumi Fushita Imyra maíra martins de Souza Lia martucci Amorim Valéria.Aparecida David Andrade Department of Hydrobiology Federal University of S&atilde o Carlos S&atilde o Carlos Brazil Postgraduate Program in Ecology and Natural Resources Federal Universityof S&atilde o Carlos S&atilde o Carlos Brazil

Land use trajectory analysis provides both the time and kind of land cover changes to monitor land use dynamics. Land cover change trajectories for three different dates (1965, 1989 and 2014) extracted from satellite images by vectorization of the thematic information were studied This study was carried out on a cultural landscape (S?o Carlos municipality, SP, Brazil) with three major land use-cover classes (forest, agriculture and other uses). The results showed the spatio temporal varia.ility of landscape pattern and forest and agriculture change trajectories. Analysis based on these landscape trajectories demonstrates that agriculture and forest cover changes have been caused by human activities. The results reflect the conflicting interactions between environmental and human systems in the study area. A key question is that the pressure exerted on forest land use-cover depends on the incentives that move society from a conflicting relation with a municipal territory. A more sustainable landscape transition and trajectory for S?o Carlos municipality are extremely dependent on the regulatory role of the government through strategies related to the implementation of specific categories of legally protected areas (Legal Reserves and Areas of Permanent Preservation).

关键词： Land Use-Cover Change Trajectories Landscape Sustainability Legally Protected Areas Remote Sensing

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Form-deprivation myopia induces decreased expression of bone morphogenetic protein-2, 5 in guinea pig sclera

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International Journal of Ophthalmology(English edition) 2015年第1期8卷 39-45页

作者： Qing Wang Mei-Lan Xue Gui-Qiu Zhao Mei-Guang Liu Yu-Na ma Yan ma Department of Ophthalmology the Affiliated Hospital of Qingdao University Department of Biochemistry and Molecular Biology Medical College Qingdao University

AIM: To identify the presence of various bone morphogenetic proteins(BMPs) and their receptors in normal sclera of human, rat and guinea pigs, and to determine whether their expression changed with form-deprivation myopia(FDM) in guinea pig ***: The expression of BMPs and BMP receptors were detected using reverse transcription polymerase chain reaction(RT-PCR) and immunofluorescence. Two-week-old guinea pigs were monocularly form-deprived with a translucent lens. After fourteen days induction of FDM, total RNA was isolated and subjected to RT-PCR to examine the changes of BMPs and BMP receptors in tissues from the posterior sclera. Western blotting analysis was used to investigate their changes in protein ***: Human sclera expressed m RNAs for BMP-2,-4,-5,-7,-ria.-RIB and BMP-RII. Conversely, rat sclera only expressed m RNA for BMP-7 and BMP-RIB,while the expression of BMPs and BMP receptors in guinea pigs were similar to that of humans. Human sclera also expresses BMP-2,-4,-5,-7 in protein *** days after the induction of myopia, significant decreased expressions for BMP-2 and BMP-5 in the posterior sclera of FDM-affected eyes(P man and guinea pig sclera. In the posterior sclera,expressions of BMP-2 and BMP-5 significantly decreased in FDM eyes. This finding indicates that various BMPs as components of the scleral cytokines regulating tissue homeostasis and provide evidence that alterations in the expression of BMP-2 and BMP-5 are associated with sclera remodeling during myopia induction.

关键词： form-deprivationmyopia bonemorphogenetic protein human sclera guinea pig sclera

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China E-commerce market Analysis: Forecasting and Profiling Internet User

China E-commerce Market Analysis: Forecasting and Profiling ...

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2011 IEEE International Summer Conference of Asia Pacific Business Innovation and Technology management(APBITM 2011)

作者： Liem Gai Sin M.Bus ria.Purnamasari Department of Economic and Business ma Chung University Malang East Java Indonesia

E-commerce has been increasing rapidly and will obviously be more popular in the future. The growth of internet is believed to have given an impact on e-commerce’s growth in China because approximately thirty percent of Chinese alter to be active internet users. The trend is pointed out to the increase of e- commerce transaction value. This research aims to provide an insight to the Chinese e-commerce market by performing a forecast of internet users. Age, gender, and income, as part of demographic profiles, are selected since they are considered to positively influence the internet user’s online activity. Furthermore, this research also provides an analysis of guidelines for companies that want to enter the China e-commerce market. For the purpose of this research, time series analysis using ARIma model will be used as the research method. Overall, findings assert Chinese internet users seem to grow rapidly. male users are still predicted to the dominate market although, surprisingly, the result shows that male internet users are to go down gradually by 2015. In addition, the result shows that internet users who have high income are increasing. Therefore, it is indicated that luxury products become potential products to be marketed. Considering the overall phenomenon, it is likely that China will still continue to be a prospective e-commerce market in the future.

关键词： Forecasting Profiling E-Commerce Demographic Profile ARIma

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灭活植物乳杆菌对仔猪生长性能、血液生化指标及腹泻率的影响

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饲料工业 2024年第10期45卷 26-31页

作者：胡虹刘德海冯菲马焕向凌云刘丽胡宜亮张秀江河南省科学院生物研究所有限责任公司河南郑州450008 河南省工业酶工程技术研究中心河南郑州450008 河南豫科检测有限公司河南郑州450008

试验主要研究灭活植物乳杆菌对仔猪生长性能、血液生化指标及腹泻率的影响。选用45头28日龄的健康杜×长×大三元杂交断奶仔猪,对照组饲喂基础饲粮,试验Ⅰ组在基础饲粮中添加0.3 g/kg天蚕素抗菌肽,试验Ⅱ组在基础饲粮中添加0.3 g/kg灭... 详细信息

试验主要研究灭活植物乳杆菌对仔猪生长性能、血液生化指标及腹泻率的影响。选用45头28日龄的健康杜×长×大三元杂交断奶仔猪,对照组饲喂基础饲粮,试验Ⅰ组在基础饲粮中添加0.3 g/kg天蚕素抗菌肽,试验Ⅱ组在基础饲粮中添加0.3 g/kg灭活植物乳杆菌。结果表明,试验Ⅰ组与对照组相比平均日增重(ADG)提高了15.56%(P0.05);试验Ⅱ组与对照组相比ADG提高了26.67%(P0.05);与对照组相比,试验Ⅱ组与对照组相比血样中葡萄糖含量提高了15.70%(P<0.05),尿素氮含量降低了42.70%(P<0.05)。由此可见,在基础日粮中添加灭活植物乳杆菌,对于仔猪具有良好的饲喂效果,可改善仔猪生长性能,降低仔猪腹泻率,改善仔猪健康。

关键词：灭活植物乳杆菌仔猪生长性能血液生化指标腹泻率

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EFFECT OF GINSENOSIDE ON THE ENDOCRINE GLANDS IN RATS

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吉林大学学报(医学版) 1990年第3期 211-215+315页

作者： Yin Xin, ma Xingyuan, Zhu Xiuxiong, Xu Jingda, ma Wenbin, Wang Xiaohui, Zhongzhi(Dept.of Histology and Embryology Dept.of Chemistry)

The effect of ginsenoside on the endocrine glands in rats was studied with radioimmunoassay (ria. and electron microscopy. Ginsenoside was injected intra-peritoneally for 5 successive days in a dosage of 100 mg/kg weight per *** animals were killed on the 6 th *** with the control, the concentration of serum growth hormone (GH) and follicle-stimulating hormone (FSH) were increased significantlyj the thyroid-stimulating hor mone (TSH) , triiodothyronine (T3) and tetraiodothyronine (T4)were decreased markedly) the luteinizing hormone (LH) and cortisol showed no evident change. Electron microscopy revealed that in pars distalis of the pituitary gland, the somatotrophs possessed highly developed rough endoplasmic reticuli or plenty of granules, the gonadotrophs contained vesicular rough endoplasmic reticuli, indicating both were in active secretory ***, the follicular epithelial cells of the thyroid appeared functionally *** results demonstrate that ginsenoside exerts a stim

关键词： Ginsenoside Pituitary gland Thyroid Hormones

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Biliary drainage in inoperable malignant biliary distal obstruction: A systematic review and meta-analysis

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World Journal of Gastrointestinal Surgery 2021年第5期13卷 493-506页

作者： maria.Vitória.Cury Vieira Scatimburgo Igor Braga Ribeiro Diogo Turia.i Hourneaux de Moura Vitor massaro Takamatsu Sagae Bruno Salomão Hirsch mateus Bond Boghossian Thomas R McCarty marcos EduardoLera dos Santos Tomazo Antonio Prince Franzini Wanderley marques Bernardo Eduardo GuimarãesHourneaux de Moura Gastrointestinal Endoscopy Unit Hospital das Clínicas da Faculdade de Medicina da Universidade de São PauloSão Paulo 05403-010Brazil Division of Gasteoenterology Hepatology and EndoscopyBrigham and Women's HospitalBostonMA 02115United States

BACKGROUND Endoscopic drainage remains the treatment of choice for unresectable or inoperable malignant distal biliary obstruction(MDBO).AIM To compare the safety and efficacy of plastic stent(PS)vs self-expanding metal stent(SEMS)placement for treatment of *** This meta-analysis was developed according to Preferred Reporting Items for Systematic Reviews and Meta-Analysis guidelines.A comprehensive search was performed in MEDLINE,Cochrane,Embase,Latin American and Caribbean Health Sciences Literature,and grey literature to identify randomized clinical tria.s(RCTs)comparing clinical success,adverse events,stent dysfunction rate,reintervention rate,duration of stent patency,and mean *** difference(RD)and mean difference(MD)were calculated and heterogeneity was assessed with I2 *** analyses were performed by SEMS *** Twelve RCTs were included in this study,totaling 1005 *** was no difference in clinical success(RD=-0.03,95%confidence interval[CI]:-0.01,0.07;I 2=0%),rate of adverse events(RD=-0.03,95%CI:-0.10,0.03;I2=57%),and mean patient survival(MD=-0.63,95%CI:-18.07,19.33;I2=54%)between SEMS vs PS ***,SEMS placement was associated with a lower rate of reintervention(RD=-0.34,95%CI:-0.46,-0.22;I2=57%)and longer duration of stent patency(MD=125.77 d,95%CI:77.5,174.01).Subgroup analyses revealed both covered and uncovered SEMS improved stent patency compared to PS(RD=152.25,95%CI:37.42,267.07;I2=98%and RD=101.5,95%CI:38.91,164.09;I2=98%;respectively).Stent dysfunction was higher in the covered SEMS group(RD=-0.21,95%CI:-0.32,-0.1;I²=205%),with no difference in the uncovered SEMS group(RD=-0.08,95%CI:-0.56,0.39;I²=87%).CONCLUSION While both stent types possessed a similar clinical success rate,complication rate,and patient-associated mean survival for treatment of MDBO,SEMS were associated with a longer duration of stent patency compared to PS.

关键词： Biliary tract neoplasms Stents Protheses malignancy Biliary system Cancer

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Antimicrobial Activity of Submerged Culture Extracts from Xylaria.stria.a

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Plant Diseases and Pests 2014年第5期5卷 11-14,37页

作者： Liu Xia Jiang Xiaolan ma Chao Yuan Xiaohong He Xinsheng ma Lin School of Life Science and Engineering Southwest University of Science and Technology

[ Objective ] The paper was to explore the antimicmbial activities of extracts from Xylaria.stria.a via submerged culture. [ Methods ] The antibacteria. activities of X. stria.a extracts against 10 kinds of bacteria.were determined using Oxford cup method and mJcroplate assay, and the antifungal activities against nine kinds of pathogenic fungi were determined using mycelial growth rate method. [ Results ] The inhibition zone diameters of the mycelium extract against tested bacte- ria.ranged from 13.0 to 17.5 mm, and that of the fermentation broth extract ranged from 10.5 to 12.7 ram. Enterobacter cloacae was the most sensitive to both my- celium extract and fermentation broth extract, with the inhibition zone diameters of 17.1 and 12.7 mm and the minimum inhibitory concentrations of 2.5 and 5 mg/mL, respectively. The inhibitory effects of the mycelium extract and the fermentation broth extract against Fusarium graminearum were the greatest, and the in- hibition rates were 89.88% and 69.53%, respectively. [ Conclusion] X. stria.a has potential antimicrobial activity and deserves to be further studied.

关键词： Xylaria.stria.a Antimicrobial activity Submerged culture

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Agisoft Photoscan在历史建筑保护中的创新应用和研究——以四川崇州市街子古镇字库塔为例

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技术与市场 2020年第7期27卷 44-46页

作者：刘红英杨春龙马利亚西华大学乡土建筑工作室四川成都610039

Photoscan影像技术的出现,为历史建筑的数据测绘提供了更简便的方式。在历史建筑的保护上却鲜有实际操作的案例研究。以崇州街子字库塔为案例,探讨将其应用在历史建筑中所面临的一些问题,希望总结出系统的应用方法,以期为传统技术测绘... 详细信息

Photoscan影像技术的出现,为历史建筑的数据测绘提供了更简便的方式。在历史建筑的保护上却鲜有实际操作的案例研究。以崇州街子字库塔为案例,探讨将其应用在历史建筑中所面临的一些问题,希望总结出系统的应用方法,以期为传统技术测绘工作提供更便捷的技术支持。

关键词： Photoscan 数字技术数据信息库历史建筑保护与更新

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