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Base editing-mediated targeted evolution of ACCase for herbicide-resistant rice mutants

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Journal of Integrative Plant Biology 2022年第11期64卷 2029-2032页

作者： Hongzhi wang Yuxin He Yingying wang Zuren Li Jiannan Hao Yijiao Song mugui wang Jian-Kang Zhu Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences Chinese Academy of SciencesShanghai 201602China University of Chinese Academy of Sciences Beijing 100049China Hunan Provincial Key Laboratory for Biology and Control of Weeds Hunan Academy of Agricultural SciencesChangsha 410125China College of Life Sciences Nanjing Agricultural UniversityNanjing 210095China Institute of Advanced Biotechnology and School of Life Sciences Southern University of Science and TechnologyShenzhen 518055China Center for Advanced Bioindustry Technologies Chinese Academy of Agricultural SciencesBeijing 100081China

Base editing technologies enable precise base alterations in a target gene without inducing double-stranded breaks,and thus are powerful for targeted gene evolution in vivo(Zhan et al.,2021).The newly evolved cytosine deaminases such as evoAPOBEC1,evoFERNY and evoCDA1,adenine deaminase TadA8e,and a near-protospacer adjacent motif(PAM)less SpRYCas9 variant,have greatly increased base editing efficiency and expanded the editing window in plants(Zhang et al.,2021;Xu et al.,2021c;Tan et al.,2022).Dual cytosine and adenine editors enable concurrent C-to-T and A-to-G conversions and thus enrich the editing *** plant dual editors have been developed(Li et al.,2020;Xu et al.,2021a),they are rarely applied in evolving plant genes due to low efficiencies.

关键词： evolution enable editing

Multiplex gene editing in rice with simplified CRISPR-Cpf1 and CRISPR-Cas9 systems

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Journal of Integrative Plant Biology 2018年第8期60卷 626-631页

作者： mugui wang Yanfei Mao Yuming Lu Zhidan wang Xiaoping Tao Jian-Kang Zhu Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences Shanghai 201602 China Department of Horticulture and Landscape Architecture Purdue University West Lafayette IN 47907 USA

The class 2 clustered regularly interspaced short palindromic repeat （CRISPR） systems have been widely used for simultaneous modification of multiple loci in plants. Traditionally, the type II CRISPR-Cas9 or type V CRISPR-Cpfl （also known as Cas12a） system is a two-component transcriptional unit （TCTU） in which the Cas9 or Cpf1 protein is expressed from an RNA polymerase （pol） II promoter, whereas the single guide RNA （sgRNA） is typically expressed from a Pol III promoter, such as U6 or U3 promoter.

关键词： Multiplex gene editing in rice with simplified CRISPR-Cpf1 and CRISPR-Cas9 systems PAM Figure LEA

Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System

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Molecular Plant 2017年第7期10卷 1011-1013页

作者： mugui wang Yanfei Mao Yuming Lu Xiaoping Tao Jian-kang Zhu Shanghai Center for Plant Stress Biology and Center for Excellence inMolecular Plant Sciences Chinese Academy of Sciences Shanghai 201602 China Department of Horticulture and Landscape Architecture Purdue University West Lafayette IN 47907 USA

Dear Editor, The class 2/type Ⅱ clustered regularly interspaced short palindromic repeat （CRISPR）/Cas9 system has been used successfully for simultaneous modification of multiple loci in plants. Two general strategies have been applied to coexpress multiple single guide RNAs （sgRNAs） to achieve multiplex gene editing in plant cells.

关键词： TARGETED MUTAGENESIS CPF1

Gene Targeting by Homology-Directed Repair in Rice Using a Geminivirus-Based CRISPR/Cas9 System

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Molecular Plant 2017年第7期10卷 1007-1010页

作者： mugui wang Yuming Lu Jose Ramon Botella Yanfei Mao Kai Hua Jian-kang Zhu Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences Shanghai 201602 China Plant Genetic Engineering Laboratory School of Agriculture and Food Sciences University of Queensland Brisbane QLD 4072 Austraria Department of Horticulture and Landscape Architecture Purdue University West Lafayette IN 47907 USA

Dear Editor, Rice （Oryza sativa） is the staple food for more than half of the world＇s population. Technologies enabling precise and efficient DNA knock-in or replacement, hereinafter referred to as KI, have the pot... 详细信息

关键词： DNA REPLICONS GENOME

Cut-dip-budding delivery system enables genetic modifications in plants without tissue culture

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The Innovation 2023年第1期4卷 1-8页

作者： Xuesong Cao Hongtao Xie Minglei Song Jinghua Lu Ping Ma Boyu Huang mugui wang Yifu Tian Fan Chen Jun Peng Zhaobo Lang Guofu Li Jian-Kang Zhu Shanghai Center for Plant Stress Biology Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China Shandong Shunfeng Biotechnology Co.Ltd. Jinan 250000China University of the Chinese Academy of Sciences Beijing 100049China Institute of Advanced Biotechnology and School of Life Sciences Southern University of Science and TechnologyShenzhen 518055China Center for Advanced Bioindustry Technologies Chinese Academy of Agricultural SciencesBeijing 100081China Hainan Yazhou Bay Seed Laboratory Sanya 572025China

Of the more than 370000 species of higher plants in nature,fewer than 0.1%can be geneticallymodified due to limitations of the current gene delivery *** for those that can be genetically modified,the modification involves a tedious and costly tissue culture ***,we describe an extremely simple cut-dip-budding(CDB)delivery system,which uses Agrobacterium rhizogene to inoculate explants,generating transformed roots that produce transformed buds due to root *** have successfully used CDB to achieve the heritable transformation of plant species inmultiple plant families,including two herbaceous plants(Taraxacum kok-saghyz and Coronilla varia),a tuberous root plant(sweet potato),and three woody plant species(Ailanthus altissima,Aralia elata,and Clerodendrum chinense).These plants have previously been difficult or impossible to transform,but the CDB method enabled efficient transformation or gene editing in them using a very simple explant dipping protocol,under non-sterile conditions and without the need for tissue *** work suggests that large numbers of plants could be amenable to genetic modifications using the CDB method.

关键词： process. enable transformed

SWO1 modulates cell wall integrity under salt stress by interacting with importinɑin Arabidopsis

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Stress Biology 2021年第1期1卷 98-119页

作者： Zhidan wang mugui wang Changhong Yang Lun Zhao Guochen Qin Li Peng Qijie Zheng Wenfeng Nie Chun-Peng Song Huazhong Shi Jian-Kang Zhu Chunzhao Zhao Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China University of Chinese Academy of Sciences Beijing 100049China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China Department of Horticulture College of Horticulture and Plant ProtectionYangzhou UniversityYangzhou 225009China Key Laboratory of Plant Stress Biology School of Life SciencesHenan UniversityKaifeng 475001China Department of Chemistry and Biochemistry Texas Tech UniversityLubbockTX 79409USA

Maintenance of cell wall integrity is of great importance not only for plant growth and development,but also for the adaptation of plants to adverse ***,how the cell wall integrity is modulated under salt stress is still poorly ***,we report that a nuclear-localized Agenet domain-containing protein SWO1(SWOLLEN 1)is required for the maintenance of cell wall integrity in Arabidopsis under salt *** in SWO1 gene results in swollen root tips,disordered root cell morphology,and root elongation inhibition under salt *** swo1 mutant accumulates less cellulose and pectin but more lignin under high ***-seq and ChIP-seq assays reveal that SWO1 binds to the promoter of several cell wall-related genes and regulates their expression under saline *** study indicates that SWO1 interacts with importinɑIMPA1 and IMPA2,which are required for the import of nuclear-localized *** impa1 impa2 double mutant also exhibits root growth inhibition under salt stress and mutations of these two genes aggravate the salt-hypersensitive phenotype of the swo1 *** together,our data suggest that SWO1 functions together with importinɑto regulate the expression of cell wall-related genes,which enables plants to maintain cell wall integrity under high salinity.

关键词： Agenet domain Salt stress Importins Plant cell wall Arabidopsis

The reformation of catalyst:From a trial-and-error synthesis to rational design

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Nano Research 2024年第4期17卷 3261-3301页

作者： Ligang wang Jiabin Wu Shunwu wang Huan Liu Yao wang Dingsheng wang Department of Chemistry Tsinghua UniversityBeijing 100084China

The appropriate catalysts can accelerate the reaction rate and effectively boost the efficient conversion of various molecules,which is of great importance in the study of chemistry,chemical industry,energy,materials and environmental ***,efficient,environmentally friendly,and easy to operate synthesis methods have been used to prepare various types of *** previous studies have reported the synthesis and characterization of the aforementioned catalysts,more still remain in trial and error methods,without in-depth consideration and improvement of traditional synthesis ***,we comprehensively summarize and compare the preparation methods of the trial-and-error synthesis strategy,structure–activity relationships and density functional theory(DFT)guided catalysts rational design for nanomaterials and atomically dispersed *** also discuss in detail the utilization of the nanomaterials and single atom catalysts for converting small molecules(H_(2)O,O_(2),CO_(2),N_(2),etc.)into value-added products driven by electrocatalysis,photocatalysis,and ***,the challenges and outlooks of mass preparation and production of efficient and green catalysts through conventional trial and error synthesis and DFT theory are featured in accordance with its current development.

关键词： trial-and-error synthesis structure–activity relationships density functional theory(DFT)guidance nanomaterials atomically dispersed catalysts

Numerical Analysis of PRISM-PY Calculations for Hard-and Soft-Core Generic Polymer Models

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Chinese Journal of Polymer Science 2024年第4期42卷 532-543页

作者： Qiang wang Department of Chemical and Biological Engineering Colorado State University1370 Campus DeliveryFort CollinsCO80523-1370USA

Generic polymer models capturing the chain connectivity and excluded-volume interactions between polymer segments can be classified, according to whether or not the 3D integral of the latter diverges, into hard- and soft-core models. Taking homogeneous systems of compressible homopolymer melts (or equivalently homopolymer solutions in an implicit, good solvent) in the continuum as an example, we recently compared the correlation effects on the structural and thermodynamic properties of the hard- and soft-core models given by the polymer reference interaction site model (PRISM) theory with the Percus-Yevick (PY) closure (Polymers 2023, 15, 1180). Here we analyzed in detail the numerical errors and behavior of the interchain pair correlation functions (PCFs) given by the PRISM-PY calculations of these models using an efficient numerical approach that we proposed. Our numerical approach has the least number of independent variables to be iteratively solved, analytically treats the discontinuities caused by the non-bonded pair potential (such as that of the hard spheres) and takes only the inverse Fourier transform of the interchain indirect PCF between polymer segments (which is continuous and decays towards 0 with increasing wavenumber much faster than both the interchain direct and total PCFs), and is essential for us to accurately solve the PRISM-PY theory for chain length N as large as 106. To capture the correlation-hole effect, the real-space cut-off in the PRISM calculations should be proportional to the square root of N.

关键词： Generic polymer models Soft potentials Polymer reference interaction site model theory

Linear-Quadratic Pareto Cooperative Game for Mean-Field Backward Stochastic System

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Journal of Systems Science & Complexity 2024年第3期37卷 947-964页

作者： wang Yu School of Control Science and Engineering Shandong UniversityJinan 250061China

This paper focuses on a Pareto cooperative differential game with a linear mean-field backward stochastic system and a quadratic form cost functional. Based on a weighted sum optimality method, the Pareto game is equivalently converted to an optimal control problem. In the first place,the feedback form of Pareto optimal strategy is derived by virtue of decoupling technology, which is represented by four Riccati equations, a mean-field forward stochastic differential equation(MF-FSDE),and a mean-field backward stochastic differential equation(MF-BSDE). In addition, the corresponding Pareto optimal solution is further obtained. Finally, the author solves a problem in mathematical finance to illustrate the application of the theoretical results.

关键词： Backward stochastic differential equation linear-quadratic control mean-field Pareto optimality