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Calcium Salts Enhance Activity and Azo Dye Decolourisation Capacity of Crude Peroxidase from <i>Armoracia rusticana</i>

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American Journal of Plant Sciences 2014年第2期5卷 212-218页

作者： mugdha ambatkar Usha Mukundan Plant Biotechnology Laboratory Ramniranjan Jhunjhunwala College Mumbai India

Armoracia rusticana is the commercial source of the enzyme Horseradish Peroxidase (HRP). Calcium ions play an important role in the functional conformation of HRP. The present study assesses the effect of three calcium salts viz., calcium chloride (CaCl2), calcium nitrate [Ca(NO3)2] and calcium sulphate (CaSO4) on the guaiacol activity of crude peroxidase extracted from the shoots and roots of in vitro grown plantlets of A. rusticana and their growth medium. The highest activity was observed in the shoot extracts of 25 mM CaCl2 treated plantlets (1.92 U/mL) and the root extracts of 25 mM Ca(NO3)2 supplemented plantlets (2.84 U/mL). The crude peroxidase activity of the medium containing 25 mM CaCl2 supplement was highest (0.13 U/mL). The capacity of the shoot and root extracts to decolourise a 10 ppm solution of methyl orange over 48 hours, was also tested. The decolourisation capacity was highest in the shoot extracts from CaCl2 treated plantlets (49.32%) and root extracts from Ca(NO3)2 treated plantlets (29.72%) respectively. Hence, the addition of calcium salts to growth medium enhances both peroxidase activity and decolourisation capacity of crude extracts of A. rusticana plantlets. These findings are of significance in enzymatic treatment for decolourisation of effluents containing dyestuffs.

关键词： Peroxidase Armoracia Rusticana Calcium Decolourisation Azo Dye

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Clinical Use of COX-2 Inhibitors Containing Quinoline Heterocycle as a Selective Therapeutic Agents for Complementary Medicine

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Clinical Complementary Medicine and Pharmacology 2023年第3期3卷 60-77页

作者： Megha P.ambatkar Nilesh R.Rarokar Pramod B.Khedekar Department of Pharmaceutical Sciences Rashtrasant Tukadoji Maharaj Nagpur UniversityNagpurMaharashtra 440033India

Inflammation represents an initial response of immune system and is involved in a number of biochemical *** incidents may multiply and further develop the provocative *** the past 15 years,various classes of secondary metabolites that were isolated from plant and marine sources have been described as natural cyclooxygenase(COX)*** majority of natural COX inhibitors could be used as a selective therapeutic agent for complementary medicine and clinical ***,the inflammation is commonly treated by non-steroidal anti-inflammatory drugs(NSAIDs),several medications of which,however,have been linked to renal and gastrointestinal side effects.A variety of inhibitors of COX-2 that are selective(celecoxib,rofecoxib,valdecoxib and others)have been designed as NSAIDs mostly with enhanced stomach safety *** helps to improve the compliance and functions in the geriatric patients as they have so many complications and problems associated with the *** use of complementary medicine in combination with clinical therapy might give better results than medicine *** disease condition like cancer which shows the COX-2 expressions could also have treatment related problems in such cases the selective inhibitors used as a complementary *** the other hand,elevated cardiovascular risks have brought increasing worries about the safety of using specific inhibitors of *** current review focuses on how quinoline heterocycle was used for creation of inhibitors of COX-2 since 2009 along with its clinical significance in complementary *** agents included the variety of substituents on the ring or ring attached to other *** a result,the quinoline heterocycle will be used for creating and finding anti-inflammatory COX-2 medicines.

关键词： Quinoline Heterocycle Inflammation Cyclooxygenase Natural Complementary NSAID COX-2 inhibitor

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可疑性先天性单纯视网膜色素上皮错构瘤的光学相干断层扫描检测

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世界核心医学期刊文摘（眼科学分册） 2005年第9期1卷 26-27页

作者： Shukla D. ambatkar S. Jethani J. Kim R. 韩静 Aravind Eye Hospital Postgrad. Institute of Ophthalmology 1 Anna Nagar Madurai 625 020 Tamil Nadu India Dr.

PURPOSE: To report the optical coherence tomography (OCT)-findings in two cases of presumed congenital simple retinal pigment epithelial (RPE) hamartoma. DESIGN: Observational case report. METHODS: Fundus examination, fluorescein angiography, ultrasonography, and OCT were done on two cases of simple RPE hamartoma. RESULTS: A heavily pigmented solitary macular lesion was noted in the right eye of a 22-year old man and a 55-year-old woman. The young man had a foveal lesion and a vision of 10/200; the woman with eccentric lesion had a vision of 20/20. The lesions blocked fluorescence on angiogram; sonography showed hyper-reflective nodules. OCT demonstrated full-thickness retinal replacement, complete optical shadowing, and abrupt margins of the lesion in both the cases, and vitreomacular adhesion at the temporal margin of the foveal lesion. CONCLUSIONS: Congenital simple RPE hamartoma may rarely occur at the foveal center, resulting in poor visual acuity. OCT is a useful non-invasive adjunct for diagnosis of this rare tumor and may reveal additional features like vitreoretinal adhesion.

关键词：视网膜色素上皮错构瘤中心凹黄斑病变血管造影照片玻璃体黄斑粘连眼底检查荧光素病变边缘色素性

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