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Analysis of Bifenthrin Degrading Bacteria from Rhizosphere of Plants Growing at Tannery Solid Waste

American Journal of Plant Sciences 2015年第13期6卷 2042-2050页

作者： Mukhtar Ahmed Farkhanda Jabeen Muhammad Ali Zarnab Ahmad Fayyaz Ahmed Muhammad Bilal Sarwar Salah ud Din mubbashir hassan Shah Jahan Centre of Excellence in Molecular Biology University of the Punjab Lahore Pakistan Department of Botany University of the Punjab Lahore Pakistan

Bifenthrin is an insecticide which is used to control insects, mites, and ticks. It poses a solemn en-vironmental threat and health risk to living organisms. It may be bioaccumulated or biomagnified at different trophic levels in the food chain by biota. Microbes are hidden creature of earth’s biodiversity. For isolation of bifenthrin degrading bacteria, rhizospheric soil samples of plants like Pisum sativum, Triticun aestvum, Chenopodium album were taken from tannery solid waste, Kasur, Pakistan. Enrichment culture techniques were used for the isolation of bacterial strains that showed luxurious growth on minimal growth media with bifenthrin dose was selected for biodegradation study. Bacteria were further screened out based on their morphological, biochemical parameters and degradation efficiency. Furthermore the effect of different growth factors like temperature, pH, inoculum concencentration, minimal inhibitory concentration of heavy metals and antibiotics were also studied. Bacterial strains of Xanthomonas and Bacillus sp. were identified as efficient degrading microbes. Maximum bifenthrin utilization were observed at 25°C (pH 7), with 500 μL inoculum of Bacillus sp., while Xanthomonas sp. gave optimm utilization at 30°C (pH 7) at the same inoculum volume of bacteria. The Rf values of Bacillus sp. and Xanthomonas sp. were 0.91 and 0.90 respectively, which indicated their potential to metabolize bifenthrin into nontoxic forms. These strains can be used to clean up the sites polluted with pesticides and tannery wastes when present in rhizosphere of plants.

关键词： Bifenthrin Biodegradation Tannery Solid Waste Rhizosphere

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Intelligent Agent-Based Architecture for Demand Side Management Considering Space Heating and Electric Vehicle Load

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Engineering（科研） 2014年第11期6卷 670-679页

作者： Farhan H. Malik mubbashir Ali Matti Lehtonen Department of Electrical Engineering and Automation Aalto University Espoo Finland

Contraction of resilience on generation side due to the introduction of inflexible renewable energy sources is demanding more elasticity on consumption side. It requires more intelligent systems to be implemented to maintain power balance in the grid and to fulfill the consumer needs. This paper is concerned about the energy balance management of the system using intelligent agent-based architecture. The idea is to limit the peak power of each individual household for different defined time regions of the day according to power production during those time regions. Monte Carlo Simulation (MCS) has been employed to study the behavior of a particular number of households for maintaining the power balance based on proposed technique to limit the peak power for each household and even individual load level. Flexibility of two major loads i.e. heating load (heat storage tank) and electric vehicle load (battery) allows us to shift the peaks on demand side proportionally with the generation in real time. Different parameters related to heating and Electric Vehicle (EV) load e.g. State of Charge (SOC), storage capacities, charging power, daily usage, peak demand hours have been studied and a technique is proposed to mitigate the imbalance of power intelligently.

关键词： Demand Response (DR) Electric Vehicles (EVs) Heat Storage Smart Grids

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First report on molecular characterization of Leishmania species from cutaneous leishmaniasis patients in southern Khyber Pakhtunkhwa province of Pakistan

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Asian Pacific Journal of Tropical Medicine 2017年第7期10卷 785-788页

作者： mubbashir Hussain Shahzad Munir Sultan Ayaz Bahar Ullah Khattak Taj Ali Khan Niaz Muhammad Muhammad Anees Hazir Rahman Muhammad Qasim Muhammad Ameen Jamal Irfan Ahmed Kashif Rahim Humaira Mazhar Noha Watanay Mohamed Kasbari Vector Borne Diseases Laboratory Department of MicrobiologyKohat University of Science and Technology Kohat Department of Plant Pathology Faculty of Plant ProtectionYunnan Agricultural University College of Animal Husbandry and Veterinary Sciences Abdul Wali Khan University Mardan College of Animal Science and Technology Yunnan Agricultural University Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Yunnan Agricultural University Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology Institute of Biochemistry and BiotechnologyCollege of Life SciencesBeijing Normal University US Naval Medical Research Unit-3 (NAMRU 3) ANSES Animal Health Laboratory

Objective: To report presence of Leishmania major in Khyber Pakhtunkhwa of Pakistan, where cutaneous leishmaniasis(CL) is endemic and was thought to be caused by Leishmania tropica only. Methods: Biopsy samples from 432 CL suspected patients were collected from 3 southern districts of Khyber Pakhtunkhwa during years 2011–2016. Microscopy on Giemsa stained slides were done followed by amplification of the ribosomal internal transcribed spacer 1 gene. Results: Leishmania amastigotes were detected by microscopy in 308 of 432 samples(71.3%) while 374 out of 432 samples(86.6%) were positive by ribosomal internal transcribed spacer 1 PCR. Subsequent restriction fragment length polymorphism confirmed Leishmania tropica in 351 and Leishmania major in 6 biopsy samples. Conclusions: This study is the first molecular characterization of Leishmania species in southern Khyber Pakhtunkhwa. It confirmed the previous assumptions that anthroponotic CL is the major CL form present in Khyber Pakhtunkhwa province. Furthermore, this is the first report of Leishmania major from a classical anthroponotic CL endemic focus identified in rural areas of Kohat district in southern Khyber Pakhtunkhwa.

关键词： Cutaneous leishmaniasis ITS-PCR RFLP Leishmania tropica Khyber Pakhtunkhwa

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