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Targeted gene sequencing and bioinformatics analysis of patients with gallbladder neuroendocrine carcinoma:A case report

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 239-251页

作者： Yun-Chuan Yang Zhi-Tao Chen Da-Long Wan Hui Tang mu-lin liu Department of Medical College Jinan UniversityGuangzhou 510000Guangdong ProvinceChina Department of General Surgery The First Affiliated Hospital of Bengbu Medical UniversityBengbu 233000Anhui ProvinceChina Department of Hepatobiliary Surgery Shulan(Hangzhou)Hospital Affiliated to Zhejiang Shuren University Shulan International Medical CollegeHangzhou 310022Zhejiang ProvinceChina Department of Hepatobiliary and Pancreatic Surgery The First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310003Zhejiang ProvinceChina Department of Pathology The First Affiliated Hospital of Medical School of Zhejiang UniversityHangzhou 310000Zhejiang ProvinceChina

BACKGROUND Gallbladder neuroendocrine carcinoma(NEC)represents a subtype of gallbladder malignancies characterized by a low incidence,aggressive nature,and poor *** its clinical severity,the genetic alterations,mechanisms,and signaling pathways underlying gallbladder NEC remain *** SUMMARY This case study presents a rare instance of primary gallbladder NEC in a 73-year-old female patient,who underwent a radical cholecystectomy with hepatic hilar lymphadenectomy and resection of liver segments IV-B and *** gene sequencing and bioinformatics analysis tools,including STRING,GeneMANIA,Metascape,TRRUST,Sangerbox,cBioPortal and GSCA,were used to analyze the biological functions and features of mutated genes in gallbladder *** mutations(APC,ARID2,IFNA6,KEAP1,RB1,SMAD4,TP53,BTK,GATA1,GNAS,and PRDM3)were identified,and the tumor mutation burden was determined to be 9.52 muts/Mb via targeted gene sequencing.A protein-protein interaction network showed significant interactions among the twelve mutated *** Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were used to assess mutation functions and *** results revealed 40 tumor-related pathways.A key regulatory factor for gallbladder NEC-related genes was identified,and its biological functions and features were compared with those of gallbladder *** Gallbladder NEC requires standardized *** with other gallbladder carcinomas revealed clinical phenotypes,molecular alterations,functional characteristics,and enriched pathways.

关键词： Gallbladder neuroendocrine carcinoma Targeted-gene sequencing Bioinformatics analysis case report Immunocytochemistry Case report

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Combined optical fiber interferometric sensors for the detection of acoustic emission

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Optoelectronics Letters 2008年第3期4卷 184-187页

作者： LIANG Yi-jun mu lin-lin liu Jun-feng YU Xiao-tao College of science Harbin Engineering University Harbin 150001 China

A type of combined optical fiber interferometric acoustic emission sensor is proposed. The sensor can be independent on the laser source and make light interference by matching the lengths of two arms,so it can be used to monitor the health of large structure. Theoretical analyses indicate that the system can be equivalent to the Michelson interferometer with two optical fiber loop reflectors,and its sensitivity has been remarkably increased because of the decrease of the losses of light energy. PZT is powered by DC regulator to control the operating point of the system,so the system can accurately detect feeble vibration which is generated by ultrasonic waves propagating on the surface of solid. The amplitude and the frequency of feeble vibration signal are obtained by detecting the output light intensity of interferometer and using Fourier transform technique. The results indicate that the system can be used to detect the acoustic emission signals by the frequency characteristics.

关键词： Acoustic emissions Acoustics Electric network analysis Fiber optics Fibers Fourier transforms Geodetic satellites Interferometers Interferometry Lattice vibrations Light sources Michelson interferometers Optical fibers Optical in Acoustic emission sensors Acoustic Emission signals Fiber loop reflectors Fourier Frequency characteristics Interferometric sensors Laser sources Light energy Light intensities Operating points Optical Vibration signals

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Long noncoding RNA RP4 functions as a competing endogenous RNA through miR-7-5p sponge activity in colorectal cancer

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World Journal of Gastroenterology 2018年第9期24卷 1004-1012页

作者： mu-lin liu Qiao Zhang Xiao Yuan Long Jin Li-Li Wang Tao-Tao Fang Wen-Bin Wang Department of Gastrointestinal Surgery the First Affiliated Hospital of Bengbu Medical College Department of General Surgery the First Affiliated Hospital of Xinxiang Medical University Department of General Surgery the Fourth Affiliated Hospital of Anhui Medical University

AIM To investigate the role of long noncoding RNA(lnc RNA) RP4 in colorectal *** Lentivirus-mediated lnc RNA RP4 overexpression and knockdown were performed in the colorectal cancer cell line SW480. Cell proliferation, tumor growth, and early apoptosis were evaluated by a cell counting kit-8 assay, an in vivo xenograft tumor model, and annexin V/propidium iodide staining, respectively. Analysis of the lnc RNA RP4 mechanism involved assessment of the association of its expression with mi R-7-5 p and the SH3 GLB1 gene. Western blot analysis was also performed to assess the effect of lnc RNA RP4 on the autophagy-mediated cell death pathway and phosphatidylinositol-3-kinase(PI3 K)/Akt *** Cell proliferation, tumor growth, and early apoptosis in SW480 cells were negatively regulated by lnc RNA RP4. Functional experiments indicated that lnc RNA RP4 directly upregulated SH3 GLB1 expression by acting as a competing endogenous RNA(ce RNA) for mi R-7-5 p. This interaction led to activation of the autophagy-mediated cell death pathway and de-repression of PI3 K and Akt phosphorylation in colorectal cancer cells in *** Our results demonstrated that lnc RNA RP4 is a ce RNA that plays an important role in the pathogenesis of colorectal cancer, and could be a potential therapeutic target for colorectal cancer treatment.

关键词： Colorectal cancer long noncoding RNA RP4 SH3GLB1 miR-7-5p competing endogenous RNA

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Pancreatic stent improves the success rate of needle-knife papillotomy in patients with difficult biliary cannulation

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World Journal of Gastroenterology 2025年第1期31卷 58-67页

作者： mu-Hsien Lee Cheng-Hui lin Chi-Huan Wu Yung-Kuan Tsou Kai-Feng Sung Sheng-Fu Wang Nai-Jen liu Department of Gastroenterology and Hepatology Chang Gung Memorial Hospital and Chang Gung University College of MedicineTaoyuan 33305Taiwan School of Medicine Chang Gung UniversityTaoyuan 33305Taiwan

BACKGROUND Needle-knife precut papillotomy(NKP)is typically performed ***,it remains unclear whether pancreatic stent(PS)placement can improve the outcomes of *** To explore whether PS placement improves the success rate of NKP in patients with difficult biliary *** This single-center retrospective study included 190 patients who underwent NKP between January 2017 and December 2021 after failed conventional biliary *** cases with incidental pancreatic duct cannulation during conventional biliary cannulation,the decision for pre-NKP PS placement was made at the endoscopist's *** primary outcome was the difference in the NKP success rate between patients with and without PS placement;the secondary outcome was the adverse event *** Among the 190 participants,82 received pre-NKP PS(PS-NKP group)whereas 108 did not[freehand or freehand NKP(FH-NKP)group].Post-NKP selective biliary cannulation was successful in 167(87.9%)patients,and the PS-NKP had a significantly higher success rate than the FH-NKP group(93.9%vs 83.3%,P=0.027).The overall adverse event rates were 7.3%and 11.1%in the PS-NKP and FH-NKP groups,respectively(P=0.493).A periampullary diverticulum(PAD)and significant intraoperative bleeding during NKP were independently associated with NKP failure;however,a pre-NKP PS was the only predictor of NKP *** the 44 participants with PADs,the PS-NKP group had a non-significantly higher NKP success rate than the FH-NKP group(87.5%and 65%,respectively;P=0.076).CONCLUSION PS significantly improved the success rate of NKP in patients with difficult biliary cannulation.

关键词： Difficult biliary cannulation Endoscopic retrograde cholangiopancreatography Needle-knife papillotomy Pancreatic stent Selective biliary cannulation

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大鼠急性坏死性胰腺炎NF-κB的变化及乌司他丁的调控作用

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世界华人消化杂志 2005年第22期13卷 2700-2703页

作者：刘瑞林刘牧林马良龙吴薇蚌埠医学院附属医院胃肠外科安徽省蚌埠市233004 淮北市人民医院肝胆外科安徽省淮北市235000

目的:探讨乌司他丁对急性坏死性胰腺炎大鼠核因子-κ B(NF-κB)的表达及有关细胞因子的影响．方法:将30只Sprague-Dawley大鼠随机分为假手术组(SO 组)、急性坏死性胰腺炎组(ANP组)和急性坏死性胰腺炎乌司他丁治疗组(UTI组),比较各组大鼠... 详细信息

目的:探讨乌司他丁对急性坏死性胰腺炎大鼠核因子-κ B(NF-κB)的表达及有关细胞因子的影响．方法:将30只Sprague-Dawley大鼠随机分为假手术组(SO 组)、急性坏死性胰腺炎组(ANP组)和急性坏死性胰腺炎乌司他丁治疗组(UTI组),比较各组大鼠NF-κB、IL-6、 TNF-α、血清淀粉酶的水平．结果:ANP组淀粉酶活性195 907．51±38 618．39 nkat／L, TNF-α值41.37±7．54 ng／L,IL-6值32．32±8．62 ng/L,肺、胰腺组织NF-κB表达增加,与SO组比较P均<0．01,乌司他丁治疗后,淀粉酶活性69 804．30±19 432．55 nkat／L,TNF-α值 18．66±6．45 ng／L,IL-6值23．41±7．65 ng／L,肺、胰腺组织NF- κB表达降低,与SO组和ANP组比较P均<0．01．结论:急性坏死性胰腺炎时NF-κB活化,上调IL-6、TNF-α, 加重胰腺及肺的损伤,乌司他丁抑制NF-κB表达而减轻胰腺及肺的损伤,改善急性坏死性胰腺炎的预后．

关键词：急性坏死性胰腺炎乌司他丁 NF-κB 肺损伤 IL-6 TNF-α 血清淀粉酶

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工程车辆储能式混合动力系统优化设计仿真

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计算机仿真 2017年第6期34卷 162-168页

作者：于忠杰林慕义刘志欣赵理北京信息科技大学机电工程学院北京100192 北京电动车辆协同创新中心北京100192

针对当前储能式混合动力工程车辆在进行系统设计及优化过程中,并未根据其工步特点及运行规律进行个性化设计而导致的系统动态特性及经济性难以达到最优化等问题,提出一种工步识别的复合储能式混合动力系统。上述系统由能量转换装置、液... 详细信息

针对当前储能式混合动力工程车辆在进行系统设计及优化过程中,并未根据其工步特点及运行规律进行个性化设计而导致的系统动态特性及经济性难以达到最优化等问题,提出一种工步识别的复合储能式混合动力系统。上述系统由能量转换装置、液压蓄能器和蓄电池构成,通过引入多个能量存储单元以同时满足高功率密度和高能量密度的要求,利用工步分析挖掘装载机工作循环的运行规律,按所处工步特点及系统状态进行控制策略设计及参数匹配。通过对系统进行结构设计、数学建模、参数匹配及仿真分析发现,混合动力系统具有较好的动态特性和经济性,较好地提高了现有装载机储能系统的整体性能。

关键词：复合储能式混合动力系统结构设计参数匹配

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Flexible Graphene Field‑Effect Transistors and Their Application in Flexible Biomedical Sensing

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Nano-Micro Letters 2025年第2期17卷 252-313页

作者： Mingyuan Sun Shuai Wang Yanbo Liang Chao Wang Yunhong Zhang Hong liu Yu Zhang lin Han Institute of Marine Science and Technology Shandong UniversityQingdao 266237ShandongPeople’s Republic of China State Key Laboratory of Crystal Materials Shandong UniversityJinan 250100ShandongPeople’s Republic of China School of Integrated Circuits Shandong UniversityJinan 250100ShandongPeople’s Republic of China Shandong Engineering Research Center of Biomarker and Artificial Intelligence Application Jinan250100ShandongPeople’s Republic of China

Flexible electronics are transforming our lives by making daily activities more *** to this innovation are field-effect transistors(FETs),valued for their efficient signal processing,nanoscale fabrication,low-power consumption,fast response times,and ***,known for its exceptional mechanical properties,high electron mobility,and biocompatibility,is an ideal material for FET channels and *** combination of graphene and FETs has given rise to flexible graphene field-effect transistors(FGFETs),driving significant advances in flexible electronics and sparked a strong interest in flexible biomedical ***,we first provide a brief overview of the basic structure,operating mechanism,and evaluation parameters of FGFETs,and delve into their material selection and patterning *** ability of FGFETs to sense strains and biomolecular charges opens up diverse application *** specifically analyze the latest strategies for integrating FGFETs into wearable and implantable flexible biomedical sensors,focusing on the key aspects of constructing high-quality flexible biomedical ***,we discuss the current challenges and prospects of FGFETs and their applications in biomedical *** review will provide valuable insights and inspiration for ongoing research to improve the quality of FGFETs and broaden their application prospects in flexible biomedical sensing.

关键词： Flexible Graphene Field-effect transistor Wearable Implantable Biosensor

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Exosomes originating from neural stem cells undergoing necroptosis participate in cellular communication by inducing TSC2 upregulation of recipient cells following spinal cord injury

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Neural Regeneration Research 2025年第11期20卷 3273-3286页

作者： Shiming Li Jianfeng Li Guoliang Chen Tao lin Penghui Zhang Kuileung Tong Ningning Chen Shaoyu liu Shenzhen Key Laboratory of Bone Tissue Repair and Translational Research Department of Orthopedic SurgeryThe Seventh Affiliated HospitalSun Yat-sen UniversityShenzhenGuangdong ProvinceChina Department of Orthopedic Surgery The First Affiliated Hospital of Jinan UniversityGuangzhouGuangdong ProvinceChina Department of Orthopedics and Traumatology Zhujiang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina Department of Orthopedic Surgery The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Department of Orthopedic Surgery The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina

We previously demonstrated that inhibiting neural stem cells necroptosis enhances functional recovery after spinal cord *** exosomes are recognized as playing a pivotal role in neural stem cells exocrine function,their precise function in spinal cord injury remains *** investigate the role of exosomes generated following neural stem cells necroptosis after spinal cord injury,we conducted singlecell RNA sequencing and validated that neural stem cells originate from ependymal cells and undergo necroptosis in response to spinal cord ***,we established an in vitro necroptosis model using neural stem cells isolated from embryonic mice aged 16-17 days and extracted *** results showed that necroptosis did not significantly impact the fundamental characteristics or number of *** sequencing of exosomes in necroptosis group identified 108 differentially expressed messenger RNAs,104 long non-coding RNAs,720 circular RNAs,and 14 microRNAs compared with the control *** of a competing endogenous RNA network identified the following hub genes:tuberous sclerosis 2(Tsc2),solute carrier family 16 member 3(Slc16a3),and forkhead box protein P1(Foxp1).Notably,a significant elevation in TSC2 expression was observed in spinal cord tissues following spinal cord ***2-positive cells were localized around SRY-box transcription factor 2-positive cells within the injury ***,in vitro analysis revealed increased TSC2 expression in exosomal receptor cells compared with other *** assessment of cellular communication following spinal cord injury showed that Tsc2 was involved in ependymal cellular communication at 1 and 3 days post-injury through the epidermal growth factor and midkine signaling *** addition,Slc16a3 participated in cellular communication in ependymal cells at 7 days post-injury via the vascular endothelial growth factor and macrophage migration inhibitory factor signaling

关键词： cellular communication competing endogenous RNA exosomes Foxp1 necroptosis neural stem cells Slc16a3 spinal cord injury transcriptome sequencing Tsc2

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NOX4 exacerbates Parkinson's disease pathology by promoting neuronal ferroptosis and neuroinflammation

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Neural Regeneration Research 2025年第7期20卷 2038-2052页

作者： Zhihao lin Changzhou Ying Xiaoli Si Naijia Xue Yi liu Ran Zheng Ying Chen Jiali Pu Baorong Zhang Department of Neurology The Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang ProvinceChina

Parkinson's disease is primarily caused by the loss of dopaminergic neurons in the substantia nigra ***,a novel form of regulated cell death characterized by iron accumulation and lipid peroxidation,plays a vital role in the death of dopaminergic ***,the molecular mechanisms underlying ferroptosis in dopaminergic neurons have not yet been completely *** oxidase 4 is related to oxidative stress,however,whether it regulates dopaminergic neuronal ferroptosis remains *** aim of this study was to determine whether NADPH oxidase 4 is involved in dopaminergic neuronal ferroptosis,and if so,by what *** found that the transcriptional regulator activating transcription factor 3 increased NADPH oxidase 4 expression in dopaminergic neurons and astrocytes in an 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine-induced Parkinson's disease *** oxidase 4 inhibition improved the behavioral impairments observed in the Parkinson's disease model animals and reduced the death of dopaminergic ***,NADPH oxidase 4 inhibition reduced lipid peroxidation and iron accumulation in the substantia nigra of the Parkinson's disease model ***,we found that NADPH oxidase 4 interacted with activated protein kinase Cαto prevent ferroptosis of dopaminergic ***,by lowering the astrocytic lipocalin-2 expression,NADPH oxidase 4 inhibition reduced 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine-induced *** findings demonstrate that NADPH oxidase 4 promotes ferroptosis of dopaminergic neurons and neuroinflammation,which contribute to dopaminergic neuron death,suggesting that NADPH oxidase 4 is a possible therapeutic target for Parkinson's disease.

关键词： dopaminergic neuron ferroptosis NADPH oxidase 4(NOX4) neuroinflammation Parkinson's disease

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Sulfolane‑Based Flame‑Retardant Electrolyte for High‑Voltage Sodium‑Ion Batteries

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Nano-Micro Letters 2025年第2期17卷 498-516页

作者： Xuanlong He Jie Peng Qingyun lin Meng Li Weibin Chen Pei liu Tao Huang Zhencheng Huang Yuying liu Jiaojiao Deng Shenghua Ye Xuming Yang Xiangzhong Ren Xiaoping Ouyang Jianhong liu Biwei Xiao Jiangtao Hu Qianling Zhang Graphene Composite Research Center College of Chemistry and Environmental EngineeringShenzhen UniversityShenzhen 518060People’s Republic of China Center of Electron Microscopy State Key Laboratory of Silicon and Advanced Semiconductor MaterialsSchool of Materials Science and EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China GRINM(Guangdong)Research Institute for Advanced Materials and Technology FoshanGuangdong 528051People’s Republic of China College of Energy Engineering Zhejiang UniversityHangzhouZhejiang 310027People’s Republic of China School of Materials Science and Engineering Xiangtan UniversityXiangtan 411105People’s Republic of China Shenzhen Eigen-Equation Graphene Technology Co.Ltd Shenzhen 518000People’s Republic of China

Sodium-ion batteries hold great promise as next-generation energy storage ***,the high instability of the electrode/electrolyte interphase during cycling has seriously hindered the development of *** particular,an unstable cathode–electrolyte interphase(CEI)leads to successive electrolyte side reactions,transition metal leaching and rapid capacity decay,which tends to be exacerbated under high-voltage ***,constructing dense and stable CEIs are crucial for high-performance *** work reports localized high-concentration electrolyte by incorporating a highly oxidation-resistant sulfolane solvent with non-solvent diluent 1H,1H,5H-octafluoropentyl-1,1,2,2-tetrafluoroethyl ether,which exhibited excellent oxidative stability and was able to form thin,dense and homogeneous *** excellent CEI enabled the O3-type layered oxide cathode NaNi_(1/3)Mn_(1/3)Fe_(1/3)O_(2)(NaNMF)to achieve stable cycling,with a capacity retention of 79.48%after 300 cycles at 1 C and 81.15%after 400 cycles at 2 C with a high charging voltage of 4.2 *** addition,its nonflammable nature enhances the safety of *** work provides a viable pathway for the application of sulfolane-based electrolytes on SIBs and the design of next-generation high-voltage electrolytes.

关键词： Sodium-ion batteries Sulfolane-based electrolyte High voltage Layered oxide cathode Flame retardant

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