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Ureteroscopy with Holmium: YAG Laser—A Initial Study in the Urology Department of the Pr Bocar Sidy Sall University Hospital of Kati

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Open Journal of Urology 2024年第4期14卷 207-216页

作者： Amadou Kassogue Idrissa Sissoko Daouda Sangare moussa Salifou Diallo Hamed Sylla Mahamadou traore Boureima Coulibaly Salia Coulibaly Modibo Diakite Modibo Togola Fadima Tall Ilias Guindo Ben Naoum Kamel Mamadou Lamine Diakite Urology Department University Hospital of Pr Bocar Sidy Sall Kati Mali Radiology Department University Hospital of Pr Bocar Sidy Sall Kati Mali Anesthesy and Reanimation Department University Hospital of Pr Bocar Sidy Sall Kati Mali Urology Department University Hospital Nimes France Urology Department University Hospital of Point G Bamako Mali

Introduction: Ureteroscopy is a minimally invasive endoscopic surgery which provides access to the ureter, pyelon and calyceal cavities via the urethra and the bladder. Laser ureteroscopy uses the laser as an energy source to treat the stone and eliminate it naturally. Minimally invasive endoscopic methods are struggling to become popular in sub-Saharan African countries, especially for the upper urinary tract. The objective of our work was to report the results of our first laser ureteroscopy experience in the department. Materials and Methods: This was a prospective and descriptive study running from December 1, 2023 to February 19, 2024. Included in our study was any case of upper urinary tract stone operated by Laser ureteroscopy. The characteristics of the lithiasis were determined by CT scan. Sterilization of urine was verified by carrying out a cytobacteriological examination of urine. Ureteral lithiasis was approached by semi-rigid ureteroscopy. Renal lithiasis was immediately addressed by flexible ureteroscopy. Ureteroscopy was coupled with a Holmium YAG laser. A double J ureteral catheter was placed after the operation. A 230 µm laser fiber was used in each case with a generator with a power of 35 watts (Storz Calculase III type). An access sheath was used in all cases of flexible ureteroscopy. The parameters studied were: sociodemographic characteristics, lithiasis (site, size, number, density, topography), type of anesthesia, duration of laser use, duration of intervention, postoperative outcomes. Data entry and analysis were carried out using the software (Word 2016 and SPSS). Result: We collected 30 cases of laser ureteroscopy. The average age was 37 years with extremes of 9 and 79 years. The male gender was more represented. The most common age group was 24-39 years old. Renal colic was the most frequent reason for admission, 12 patients (40%). On physical examination, lumbar tenderness was present in 47% (14 patients). ECBU was positive in 4 patients

关键词： Ureteroscopy Semi-Rigid Flexible Holmium YAG Laser

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Epidemiological and Clinical Characteristics of Renal Diseases Diagnosed by Biopsies in Ivory Coast

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Open Journal of Pathology 2022年第3期12卷 100-113页

作者： Kouame Justin N’Dah Weu Melanie Tia Alain Didier Abouna Ibrahiman Toure Amelie Delphine Lagou Monley Cyr Guei Ibrahim Cherif moussa traore Eyram Amekoudi Attaah Ange Rebecca Kobenan Sery Patrick Olivier Diopo Kouame Hubert Yao N’Guessan Clement Ackoundou Koffi Laurence Adonis Dazé Apolinaire Gnionsahié Mohenou Isidore Jean Marie Diomande Department of Anatomic Pathology University Teaching Hospital of Bouake Bouake Ivory Coast Department of Nephrology University Teaching Hospital of Bouake Bouake Ivory Coast Department of Anatomic Pathology University Teaching Hospital of Cocody Abidjan Ivory Coast Department of Nephrology University Teaching Hospital of Yopougon Abidjan Ivory Coast Department of Nephrology University Teaching Hospital of Donka Conakry Guinea Department of Nephrology Sylvanus Olympio University Teaching Hospital Lomé Togo Department of Nephrology University Teaching Hospital of Treichville Abidjan Ivory Coast Department of Pediatric Nephrology University Teaching Hospital of Yopougon Abidjan Ivory Coast

Renal biopsy is an invasive procedure used to evaluate the activity and the therapeutic management of kidney disease and kidney transplantation. Objective: The aim of this study was to describe the epidemiological, clinical, and pathological features of kidney disease diagnosed by biopsy in the Ivory Coast. Materials and Methods: This was a descriptive and prospective study conducted between January 2015 and December 2018 in the Department of Anatomy and Cytology of the Pathology of Cocody (Abidjan) and Bouake. Specimens were obtained from the nephrology department of Ivory Coast and from teaching hospitals in Togo, Guinea, Burkina Faso, and Mali. Samples were analyzed according to standard renal biopsy procedures. One kidney fragment was fixed in acetic acid formalin (AAF) for optical microscopy examination. The other fragment, soaked in physiological water, was immediately sent for immunofluorescence examination. All renal pathologies were included in this study. The study parameters were frequency, age, sex, origin, occupation, clinical and biological signs, and pathological aspects with optical microscopy, and immunofluorescence. Results: 153 cases (2.23%) of kidney biopsies were examined on a total of 10,573 specimens. Ivory Coast specimens accounted for 91.5% of cases (n = 140). Nephrotic syndrome (49%) was the most common clinical sign and indication for renal biopsy, followed by acute renal failure (16.3%), chronic renal failure (19.6%), and a combination of chronic renal failure with hypertension (11.18%) and glomerulonephritis (4%). Mean proteinuria was 3.03 g/24h (range, 0.14 to 11.5 g/24h). Histologically, 90.8% (n = 139) were glomerular nephropathies, including 26.6% HIV-associated nephropathy, 17.3% focal segmental glomerulosclerosis, 13.6% nephroangiosclerosis, 11.5% post-infectious glomerulonephritis, 9.3% membranous glomerulonephritis, and 21.6% miscellaneous glomerular nephropathies (n = 30). The incidence of various tubulo-interstitial lesions was

关键词： Kidney Biopsy Disease Pathology Ivory Coast

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Ear Cholesteatoma Surgery in South Sahara: Our Experience in Bamako

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International Journal of Otolaryngology and Head & Neck Surgery 2024年第4期13卷 293-303页

作者： Kassim Diarra Oumou Coulibaly Yaya Dembele Nfaly Konaté Sidibe Youssouf Saydi Ag Mohamed Harouna Sanogo Abraham Diarra Ibrahim Fofana moussa Bourama Keita Youssouf traore Fatogoma Issa Koné Boubacary Guindo Siaka Soumaoro Doumbia Kadidiatou Singare Mohamed Amadou Keita ORLCCF Department Gabriel Toure University Hospital Bamako Mali ORLCCF Unit Kati Army Polyclinics Kati Mali ENT-CCF Department CHU Luxembourg Bamako Mali ORLCCF Service of the Reference Center of Commune VI Sogoniko Bamako Mali

In Mali, cholesteatoma surgery remains a challenge due to the limited number of otological practitioners, and the low socio-economic level of patients reflecting the difficulties in performing CT or MRI for post-operative follow-up. We therefore initiated this work with the objective of analyzing, through a broad bibliographic review, the clinical, paraclinical, topographical, therapeutic and progressive aspects of a series of ear cholesteatoma. Materials and Method: This was a retrospective and prospective, descriptive study that took place in the ENT and head and neck surgery department of the Gabriel Touré University Hospital over 36 months from November 2020 to October 2023. These were patients admitted to the department for chronic cholestematous otitis media. Result: In total, we collected 34 files of patients admitted for cholesteatoma. This represented 9% of cases compared to all otological surgeries carried out during the same period, i.e. 362 cases. The average age of our patients was 35.31 years, with extremes ranging from 7 years to 80 years, there were 24 men and 10 women, i.e. a M/F ratio of 2.4. The average time to diagnosis was 7 years. The main functional signs were dominated by chronic fetid purulent otorrhea associated with hypoacusis in 94.6% of cases. One case of meningeal complication and three cases of cerebral empyema were reported. Clinically, a postero-superior and marginal tympanic perforation was observed in 53%, a retraction pocket in 5.9%, the sentinel polyp in 9%, and a non-marginal tympanic perforation in 32%. Pure-tone audiometry performed preoperatively for all patients showed conductive hearing loss in 83%. In 82% of cases we created a recess with the creation of a mini box. An ossiculoplasty was performed immediately with fragments of cartilage. The postoperative course found the disappearance of otorrhea (88.23%), the persistence of otorrhea in one case, two cases of facial paralysis, and one case of tinnitus. We did not identify

关键词： Cholesteatoma CT TTO TTF

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Molecular Diagnosis of Sexual Differentiation Disorders in Burkina Faso

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Journal of Biosciences and Medicines 2024年第3期12卷 1-15页

作者： Bazie Bapio Valérie Elvira Jean Télesphore Sawadogo Soumaïla Zohoncon Théodora Mahoukèdè Tall Ahmadou Bado Prosper Soubeiga Serge Théophile Ouattara moussa Sorgho Abel Kiendrebeogo Touwendpoulimdé Isabelle Yonli Albert Theophane Djigma Florencia Millogo/traore Timongo Françoise Danielle Simpore Jacques Centre National de la Recherche Scientifique et Technologique (CNRST) Institut de Recherche en Sciences de la Santé (IRSS) Ouagadougou Burkina Faso Université Joseph KI-ZERBO Unité de Formation en Sciences de la Vie et de la Terre Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigonie (CERBA) Ouagadougou Burkina Faso Centre Hospitalier Universitaire Régional de Ouahigouya Ouagadougou Burkina Faso Centre Hospitalier Universitaire Yalgado Ouedraogo Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Sex development anomalies represent a group of congenital pathologies in which chromosomal, gonadal and anatomical sex differentiation is atypical. The aim of our study was to use molecular biology techniques to elucidate sex in cases of anomalies of sexual differentiation in Burkina Faso. This cross-sectional study took place from March 2023 to June 2023. Oral and blood samples were collected respectively using sterile swabs and stored on Swab Kits and NUCLEIcardTM (https://***/product-ranges/nucleic-card/) were used to determine gonosome profiles. Extraction was carried out using the DNA Swap solution Kit and the DNA IQ System Kit, and a PowerPlex? 21 kit (Promega) for amplification. The Applied Biosystems 9700 thermal cycler was used for PCR followed by 36 cm capillary electrophoresis in the Applied Biosystems 3130 prism sequencer. Sequence files were analyzed using GeneMapper IDX v. 3.2 software. Seven (07) patients were registered during the study period. There were 4 cases of XX DSD or 46, XX DSD and 3 cases of XY DSD or 46, XY DSD. The median age of our patients was 16 years. Civilian sex was male in 4 cases and female in 3. The most frequent reason for consultation was micropenis in 3 cases, followed by primary amenorrhea and sex ambiguity. There were 03 cases of discrepancy between genetic sex and civil sex. The accessibility of molecular diagnosis is little known to clinicians. XX DSDs or 46, XX DSDs were the most frequent (4/7) in our study. The problem facing this situation is early diagnosis to help prevent complications in Burkina Faso.

关键词： Gonosome DSD Abnormal Sexual Development Burkina Faso

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Focal Segmental Glomerulosclerosis in Côte d’Ivoire: Epidemiological, Clinical and Pathological Aspects

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Open Journal of Pathology 2022年第4期12卷 146-155页

作者： N’Dah Kouame Justin Tia Weu Melanie Akpro Sedy Louess De Randorphe Lagou Delphine Amélie Toure Ibrahiman Kobenan Atta Anne Rebecca Abouna Alain Didier Guei Monley Cyr Tuo Wonko-Tian Alice Oka Kouamé Hubert Diopo Sery Patrick Olivier Delma Samuel Cherif Ibrahima traore moussa Amekoudi Eyram Ouattara Belarsi Safiatou Yao Kouame Hubert Ackoundou Nguessan Clément Adonis Koffi Laurence Yao Gnangoran Victor Gnionsahie Dazé Apolinaire Mohenou Isidore Jean-Marie Diomandé Department of Anatomic Pathology University Teaching Hospital of Bouake Bouake Ivory Coast Department of Nephrology University Teaching Hospital of Bouake Bouake Ivory Coast Department of Nephrology University Teaching Hospital of Yopougon Abidjan Ivory Coast Department of Anatomic Pathology University Teaching Hospital of Cocody Abidjan Ivory Coast Department of Nephrology University Teaching Hospital of Treichville Abidjan Ivory Coast Department of Nephrology University Teaching Hospital of Blaise Compaore Ouagadougou Burkina Faso Department of Anatomic Pathology University Teaching Hospital of Donka Conakry Guinea Department of Nephrology University Teaching Hospital of Sylvanus Olympio Lome Togo Department of Nephrology University Teaching Hospital of Treichville Abidjan Ivory Coast

Focal segmental glomerulosclerosis (FSGS) is characterized histologically by hyalinosis and sclerosis of glomeruli associated or not with podocyte involvement. The objective of our work was to clarify the epidemiological aspects and histological variants of FSGS in Côte d’Ivoire. Materials and Methods: This was a descriptive retrospective study, conducted from January 2015 to December 2019 using the renal biopsy registers (RB) of the Pathological Anatomy and Cytology departments of the Teaching Hospital of Cocody and Bouake in collaboration with the Nephrology Services of Côte d'Ivoire and the sub-region. The biopsies underwent conventional histopathology and/or immunofluorescence techniques. The parameters analyzed were: frequency, age, gender, proteinuria, biopsy indications and histological aspects and the different correlations between histological aspects and socio-demographic characteristics. Results: FSGS represented 58.1% (n = 104) of glomerular nephropathies. The average age of patients was 32.1 ± 13.3 years, with extremes of 13 and 70 years. The sex ratio was equal to 1. Nephrotic syndrome (68.9%), chronic renal failure (14.3%) and acute renal failure (10.1%) were the main indications for renal biopsy (RB). The mean proteinuria at the time of diagnosis was 4 ± 3.7 g/24 h. It was massive (3.5 g/24 h) in 42.3% of patients. FSGS was primary in 29.8% (n = 31) and secondary in 70.2% (n = 73) of patients, of which 27.9% (n = 35) was due to HIV. According to the Columbia classification, 62.5% NOS type was found;23.1% collapsing type;7.7% tip lesion type;4.8% cell type and 1.9% perihilary type. Conclusion: FSGS is a complex heterogeneous entity. It affects young people in our context with a homogeneous gender distribution. Understanding its histogenesis is essential for optimal patient management.

关键词： Kidney Glomerulus Histology FSGS Côte d’Ivoire

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Antibacterial, Anti-Inflammatory and Antioxidant Activities of an ACAZY Herbal Formula Used in Traditional Medicine to Treat Respiratory Infections

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Natural Science 2024年第9期16卷 131-145页

作者： Mohamed Bonewendé Belemlilga Bagora Bayala Abdoul Gilchrist Laurent Boly Madinatou Annick Ouédraogo Alida Raissa Boina Boubacar Yaro Florentina Badea Camélia Diguti Moumouni Koala Denise P. Ilboudo Charles Bavouma Sombié Pascal Nadembega Jean-Marie Compaoré Paul Darga Mahoukèdè Théodora Zohoncon moussa Ouédraogo Aristide traore Noufou Ouédraogo Florentina Matei Rasmane Semdé Sylvin Ouédraogo Jacques Simporé Innocent Pierre Guissou Laboratoire de Recherche-Dveloppement de Phytomdicaments et Mdicaments (LR-D/PM) Institut de Recherche en Sciences de la Sant (IRSS) Centre National de la Recherche Scientifique et Technologique (CNRST) Ouagadougou Burkina Faso Centre de Recherche Biomolculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Laboratoire de Biologie Molculaire et de Gntique (LABIOGENE) Universit Joseph KI-ZERBO Ouagadougou Burkina Faso Ecole Normale Suprieure Koudougou Burkina Faso Laboratoire de Dveloppement du Mdicament (LADME) Centre de Formation de Recherche et dExpertises en Sciences du Mdicament (CEA-CFOREM) cole Doctorale Sciences de la Sant (ED2S) Universit Joseph KI-ZERBO Burkina Faso Laboratoire de Microbiologie Applique Universit des Sciences Agronomiques et de Mdecine Vtrinaire (UASMV) de Bucarest en Roumanie Bucharest Romania Dpartement de la mdecine traditionnelle Ministre de la Sant et de lHygine Publique Ouagadougou Burkina Faso Universit Saint Thomas dAquin (USTA) Ouagadougou Burkina Faso

ACAZY is a plant formula used in traditional medicine in Burkina Faso to treat respiratory infections. After phytochemical analysis, this study evaluated extracts’ anti-inflammatory, antioxidant and antibacterial properties from the ACAZY recipe. Three extractions, an aqueous macerate (AM), an aqueous decoction (AD) and an hydroethanolic macerate (HEM) of the ACAZY recipe powder were carried out. Phytochemical screening of the extracts was carried out using high-performance thin-layer chromatography (HPTLC) and the determination of phenolic compounds. The anti-inflammatory potential was assessed in vitro using pro-inflammatory enzyme inhibition tests. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and Ferric-reducing antioxidant power (FRAP) antioxidant properties were also determined. The antibacterial activity was evaluated on Staphylococcus aureus and Streptococcus pneumoniae strains. Phytochemical analysis revealed the presence of flavonoids, saponins, tannins, anthracenosids, sterols and triterpenes in the extracts. The extracts inhibited pro-inflammatory enzymes by more than 40% at only 100 µg/mL. The extracts also showed potent antibacterial activity with a minimum inhibitory concentration 1 mg/mL on Staphylococcus aureus and 2 mg/mL on Streptococcus pneumoniae. The extracts in the ACAZY formula have shown anti-inflammatory and antioxidant properties in vitro. The AD also showed an antibacterial activity. This justifies its use in traditional medicine to treat acute respiratory infections.

关键词： ACAZY Anti-Inflammatory Antioxidant Antibacterial In Vitro

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Monteggia Bilateral Fracture: A Case Study

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Surgical Science 2019年第8期10卷 297-301页

作者： Louis traore moussa Sidibe Abdoulkadri moussa Tieman Coulybali Rabat Faculty of Medicine and Pharmacy Orthopedic Trauma Mohammed V University Rabat Morocco

The authors report a rare case of Monteggia bilateral fracture combining bifocal fracture of the left ulna to a mid-shaft fracture of the radius with dislocation of the radial head and on the right;a fractured ulna with dislocation of the radial head occurring in a patient of 31 years after an accident of the public highway. In emergency, the treatment consisted of a fixation with radius special plates and a left pin ulnar upper radio. The evolution was marked by a good consolidation and after six months the patient had resumed operations.

关键词： Fracture Monteggia Bilateral

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Study of Complications of Traditional Treatment. <br/>Member Fractures

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Surgical Science 2020年第10期11卷 289-297页

作者： Louis traore Laye Toure moussa Sidibe Mamadou Diallo Aboul Kadri moussa Mamadou Bassirou traore Tiéman Coulibaly Service de Chirurgie orthopédique et traumatologique CHU Gabriel Touré Bamako Mali Service de Chirurgie orthopédique et traumatologique Hopital Regional de Sikasso Bamako Mali

Introduction: Since the times of our ancestors, traditional medicine has existed to cure all diseases [1]. The objective of this study was to assess the complications of traditional fracture treatment. Patients and Methods: This was a prospective study extending from January 01, 2018 to June 30, 2018, duration of six months. It concerned patients who had traditionally been treated beforehand for traumas with bone lesion in a traditional therapist and who had developed a complication, the definitive management of which was made in the department. Results: This study involved 102 patients, including 71 men (69.6%), with a sex ratio of 2.2. 33 patients or 32.4% were under 15 years old. The average of our patients’ age was 29.61 years with extremes of 2 and 89 years. Pupils/students (44 pupils and 5 students) were the most represented with 49 cases or 48.1% of the cases. The majority of patients (59.8% of cases) were educated or had a basic level. The majority of patients (36.3%) came as motif for a painful swelling. Gangrene was the most common complication with 37 cases or 36.3% of cases. Conclusion: At the end of our study, we have noticed that the traditional preliminary treatment of fractures was based mainly on trial and error. Practicing with inadequate means and measures without respecting the anatomical structures, is a real source of disabling handicaps. We have recorded 38, among the 102 patients who came for a complication of traditional treatment: that is 45.2% amputation, which is deplorable.

关键词： Fracture Complications Traditional Treatments

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Direct Cost of Severe Malaria Treatment Borne by the Families of Children Aged 0 - 5 Years at the Fana Reference Health Centre, Mali

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Health 2022年第12期14卷 1307-1320页

作者： Solomane traore Abdourahamane Haidara Youssouf Samake Tegué Guindo moussa Keita National Institute of Public Health (INSP) Bamako Mali Reference Health Centre (CSRéf) of Fana Koulikoro Mali

Introduction: Malaria is both a disease caused by poverty and a cause of poverty. Malaria is the leading cause of morbidity and mortality in Mali and is among the ten countries with the highest number of malaria cases and deaths. The objective was to estimate the direct economic cost borne by families in the treatment of severe malaria in children aged 0 - 5 years at the CSREF in Fana. Methodology: The study was cross-sectional, conducted from July 2017 to June 2018 with inclusion criteria and prospective data collection. The methodology was based on estimating the direct economic cost of severe malaria. Results: The sample consisted of 109 cases out of a total of 944 hospitalizations;59% of whom were boys and the 25 - 36 month age group was the most affected. The complications frequently encountered were severe anemia (50 cases) or 45.8%;convulsions (35 cases) or 32.1% and finally severe sepsis (8 cases) or 7.3%. The average direct cost was 25,324 Franc CFA (58.95 US Dollars) of which 66% represented the costs of medicines and consumables against 4% for the consultation. This cost was more than half the minimum wage in Mali. Conclusion: Despite the difficulties in estimating the cost in hospitals, the results obtained give us an estimate of the economic burden borne by families in the management of severe malaria cases among children in the district of Fana. Support is needed for parents in the fight against malaria in rural Mali.

关键词： Severe Malaria Direct Average Cost Fana District Health Center Mali

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Polycyclic Evolution of Paleoproterozoic Rocks in the Southwestern Part of the Mako Group(Eastern Senegal,West Africa)

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International Journal of Geosciences 2014年第7期5卷 739-748页

作者： Papa moussa Ndiaye Edmond Dioh Famara Diatta Alassane traore Makhoudia Fall Ousmane Balde Département de Géologie Universite Cheikh Anta DiopDakarSénégal Institut Fondamental d’Afrique Noire Universite Cheikh Anta DiopDakarSénégal Institut de Technologie Nucléaire Appliquée Universite Cheikh Anta DiopDakarSénégal

After an extensional stage giving rise to tholeiitic basalts and gabbros, the south western part of Kédougou Kéniéba Inlier (KKI) was affected by three phases of compressionnal deformation. The first phase (D1) was related to NE-SW stress that led to northeastward thrusting. Early granitoids would be implemented during this tectonic event. The second phase (D2) was due to East-West compressional stress that created combined strike sleep faults: NNW-SSE and NNE-SSW respectively sinistral and dextral. During this phase, some volcanic rocks and gabbros were mylonitized, hydrothermalized and transformed into quartzites. These quartzites were considered as sedimentary rocks in previous studies. Granitic plutons and andesites took place during this tectonic phase. D3 deformation occurred towards the end of the Eburnean orogeny. It would be held in two episodes. In the first episode, WNW-ESE stresses created conjugate shears: sinistral NNW-SSE and dextral NE-SW. In the second episode, stresses moved toward the NE-SW direction and made the resulting brittle structures arranged in NS dextral corridors.

关键词： Paleoproterozoic Eastern Senegal Volcanic-Rocks Polycyclic Deformation Silicification

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