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Identification and preliminary characterization of non-polyene antibiotics secreted by new strain of actinomycete isolated from sebkha of Kenadsa, Algeria

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Asian Pacific Journal of Tropical Biomedicine 2015年第6期5卷 438-443页

作者： Omar Messaoudi mourad bendahou Ibrahim Benamar Djamal-Elddine Abdelwouhid Microbiology Laboratory Applied to Food Biomedical and Environmental (LAMAABE) University of Abou bekr Belkaid Faculty of SNV-STU- Ex Imama Biomedical ComplexPB 119 Tlemcen 13000 Algeria Faculty of Science Department of Biology University of Amar Telidji

Objective: To study the antimicrobial activity of actinomycetes isolated from sabkha of Kenadsa and identification of the isolate interesting. Methods: Eighteen strains were isolated, using four culture media from sebkha of Kenadsa(Bechar, Southwestern Algeria). Screening of antimicrobial activity consisted of two steps: in primary screening, antibacterial activity was determined by using the agar plug method against test strains; in secondary screening, better isolate which showed a good activity in the first screening was selected to extract antimicrobial substances. The antimicrobial activities of extracts were evaluated by using Kirby-Bauer disc diffusion method. Partial characterization of antimicrobial products was performed on the basis of chemical revelations, UV-vis spectrometry and infrared spectroscopy. The identification of isolate interesting was performed through morphological, chemical, biochemical and physiological characteristics. Results: All isolates showed antimicrobial activity against at least one microorganism test. One isolate, LAM143 cG 3, was selected for its broad spectrum and high antimicrobial activity. The isolate LAM143 c G3 was identified as Spirillospora sp. The comparison between the species of this genus(Spirillospora rubra and Spirillospora albida) and our isolate indicated the existence of several physiological and biochemical differences which led us to suppose that this was a new member of this genus. Primary characterization of antimicrobial substances produced by the isolate LAM143 c G3 indicated the presence of amines and phenols. The UV–vis spectrum suggested a non-polyenic nature of substances secreted by our isolate, while infrared confirmed the presence of amine ***: The result of the present study revealed that sebkha of Kenadsa was rich in rare actinomycetes, that secreted interesting antimicrobial substance.

关键词： Isolation Actinomycetes Sebkha Kenadsa Non-polyene Identification Spirillospora

Antimicrobial activity of some essential oils against oral multidrugresistant Enterococcus faecalis in both planktonic and biofilm state

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Asian Pacific Journal of Tropical Biomedicine 2014年第6期4卷 463-472页

作者： Fethi Benbelaid Abdelmounaim Khadir Mohamed Amine Abdoune mourad bendahou Alain Muselli Jean Costa Laboratory of Applied Microbiology in Food Biomedical and Environment(LAMAABE)Aboubekr Belkaid University Laboratory of Natural Products Chemistry University of CorsicaUMR CNRS 6134Campus GrimaldiBP 5220250 CorteFrance

Objective:To evaluate some essential oils in treatment of intractable oral infections,principally caused by hiofilm of multidrug-resistant Enterococcus faecalit(***),such as persistent endodontic infections in which their treatment exhibits a real challenge for ***:Ten chemically analyzed essential oils by gas chromatography-mass spectrometry were evaluated for antimicrobial activity against sensitive and resistant clinical strains of *** in both planktonic and hiofilm state using two methods,disk diffusion and broth ***:Studied essential oils showed a good antimicrobial activity and high ability in *** biofilm eradication,whether for sensitive or multidrug-resistant strains,especially those of Origanum glandulosum and Thymbra capitata with interesting minimum inhibitory concentration,biofilm inhibitory concentration,and biofilm eradication concent ration values which doesn't exceed 0.063%,0.75%,and 1.5%,***:Findings of this study indicate that essential oils extracted from aromatic plants can be used in treatment of intractable oral infections,especially caused by biofilm of multidrugresistant ***.

关键词： Bacterial infections Biofilm Enterococcus faecalis Essential oils Multidrug-resistance

Antimicrobial activity of Rosmarinus eriocalyx essential oil and polyphenols:An endemic medicinal plant from Algeria

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Journal of Coastal Life Medicine 2016年第1期 39-44页

作者： Fethi Benbelaïd Abdelmounaïm Khadir mourad bendahou Fatima Zenati Chafika Bellahsene Alain Muselli Jean Costa Laboratory of Applied Microbiology in Food Biomedical and EnvironmentAboubekr Belkaïd UniversityP.O.Box 119Tlemcen 13000Algeria Department of Biology University of Oran 1 Ahmed Ben BellaEs Senia OranAlgeria University of Corsica Team of Chemistry of Natural ProductsUMR-CNRS 6134Quartier GrossettiBP 52Corti 20250France

Objective:To evaluate the antimicrobial potency of Rosmarinus eriocalyx(***)essential oil and total polyphenols against pathogenic ***:Antimicrobial activity of *** extracts was assessed by disc diffusion method and minimum inhibitory concentrations *** oil obtained from endemic rosemary by hydrodistillation was analysed by gas chromatograph/retention index and gas chromatograph-mass ***:An interesting antimicrobial activity was shown by *** ***,constituted mainly by flavonoids,were the most effective extract with very low minimum inhibitory concentrations values,ranged between 0.06 and 8.00 mg/mL,while essential oil was less *** should be noted that antimicrobial activities of both *** extracts were more directed against fungi and Gram-positive bacteria than Gram-negative ones,in which Staphylococcus aureus,Enterococcus faecalis,and Candida albicans were the most sensitive *** chemical composition of *** essential oil,camphor(37.8%),1,8-cineole(17.4%),camphene(13.3%),and α-pinene(10.9%)were the major ***:The findings of the present study indicate that *** extracts possess significant bactericidal and fungicidal *** of its richness in essential oil,and especially flavonoids,*** may be a source for effective and safe antimicrobial agents.

关键词： Antimicrobial activity Essential oil Polyphenols Rosmarinus eriocalyx

Structure-Thermal Conductivity Tentative Correlation for Hybrid Aerogels Based on Nanofibrillated Cellulose-Mesoporous Silica Nanocomposite

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Journal of Renewable Materials 2018年第3期6卷 299-313页

作者： Dounia bendahou Abdelkader bendahou Bastien Seantier Benedicte Lebeau Yves Grohens Hamid Kaddami University of Southern Brittany Dupuy de Lôme Research InstituteFRE-CNRS 3744BP 9211656321 Lorient CedexFrance Cadi Ayyad University Faculty of Sciences and TechnologiesAvenue Abdelkrim ElkhattabiB.P.549MarrakechMorocco University of Upper Alsace Institute of Materials Science of Mulhouse(IS2M)68057 Mulhouse CedexFrance

Hybrid aerogels have been prepared by freeze-drying technique after mixing water dispersions of cellulose microfibers or cellulose nanofibers and silica(SiO2)of type SBA-15(2D-hexagonal).The prepared composites were characterized by different analysis techniques such as SEM,hot-filament,DMA,*** composites are compared to those previously prepared using nanozeolites(NZs)as mineral *** morphology studied by SEM indicated that both systems have different structures,i.e.,individual fibers for cellulose microfibers WP-based aerogels and films for nanofibrillated cellulose NFC-based ones....These differences seem to be driven by the charge of the particles,their aspect ratio and *** hybrid materials exhibit tunable thermal conductivity and mechanical *** thermal conductivity values range between^18 to 28 mW.m^-1.K^-1 and confirm the superinsulation ability of these fibrous *** on the thermal insulation properties and mechanical properties was shown by adjunction of mineral particles to both cellulose-based aerogels by reaching pore size lower than 100 *** significantly reduces the thermal conductivity of the hybrid aerogels as predicted by Knudsen et ***,the addition of mineral fillers to aerogels based on cellulose microfibers induced a significant increase in stiffness.

关键词： Aerogels cellulose microfibers WP TEMPO-oxidized NFC silica lyophilization porosity superinsulation mechanical properties

Synthesis of Mesoporous Chromium Silicates Molecular Sieves in Strong Acidic Media by Assembly of Preformed CrS1 Precursors with Triblock Copolymer

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Journal of Chemistry and Chemical Engineering 2011年第2期5卷 177-181页

作者： Karima bendahou Saidi Fatiha Hamidi Leila Cherif Aouali Stephane Siffert Abdelkader Bengueddach Aissa Aissat Fabrice Cazier Antoine Aboukais Laboratoire de catalyse et synthese en ehimie organique PB 119 Universite de Tlemcen Algerie Univ Lille Nord de France F-59000 Lille France ULCO LCE F-59140 Dunkerque France Laboratoire de ehimie des materiaux Universite d ＇Oran Algerie ULCO CCM F-59140 Dunkerque France

Chromium-incorporated mesoporous silica materials （MCrS） have been successfully prepared in strong acidic media by assembly of preformed CrSI precursors with triblock copolymer of the Pluronic type （P123） by a two steps procedure. Various techniques including XRD, N2 adsorption, FT-IR spectroscopy, diffuse reflectance UV-vis （DRUV-vis） and EPR were employed for the materials characterization. MCrS materials have a hexagonal structure with uniform mesopores and contain Cr（V） and Cr（VI） under low and high Cr content and that the mesoporous walls contain the MFI structure building units.

关键词： Zeolite precursors CrS-1 mesoporous SBA-15