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MACS-annexin V cell sorting of semen samples with high TUNEL values decreases the concentration of cells with abnormal chromosomal content:a pilot study

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Asian Journal of Andrology 2022年第5期24卷 445-450页

作者： Sahar El Fekih Nadia Gueganic Corinne Tous Habib ben ali mounir Ajina Nathalie Douet-Guilbert Hortense Drapier Damien Beauvillard Frédéric Morel Aurore Perrin University of Brest InsermUMR U1078Faculty of Medicine and Health SciencesBrest 29238France Laboratory of Human Cytogenetics Molecular Genetics and Reproductive BiologyFarhat Hached University HospitalSousse 4031Tunisia Departement of Medical Genetics and Reproductive Biology Brest University Regional HospitalBrest 29609France Department of Reproductive Medicine Farhat Hached Teaching HospitalSousse 4031Tunisia

We question whether,in men with an abnormal rate of sperm DNA fragmentation,the magnetic-activated cell sorting(MACS)could select spermatozoa with lower rates of DNA fragmentation as well as spermatozoa with unbalanced chromosome content.Cryopreserved spermatozoa from six males were separated into nonapoptotic and apoptotic populations.We determined the percentages of spermatozoa with(i)externalization of phosphatidylserine(EPS)by annexin V-Fluorescein isothiocyanate(FITC)labeling,(ii)DNA fragmentation by TdT-mediated-dUTP nick-end labeling(TUNEL),and(iii)numerical abnormalities for chromosomes X,Y,13,18,and 21 by fluorescence in situ hybridization(FISH),on the whole ejaculate and selected spermatozoa in the same patient.Compared to the nonapoptotic fraction,the apoptotic fraction statistically showed a higher number of spermatozoa with EPS,with DNA fragmentation,and with numerical chromosomal abnormalities.Compared to the whole ejaculate,we found a significant decrease in the percentage of spermatozoa with EPS and decrease tendencies of the DNA fragmentation rate and the sum of disomy levels in the nonapoptotic fraction.Conversely,we observed statistically significant higher rates of these three parameters in the apoptotic fraction.MACS may help to select spermatozoa with lower rates of DNA fragmentation and unbalanced chromosome content in men with abnormal rates of sperm DNA fragmentation.

关键词： chromosomal abnormalities DNA fragmentation externalization of phosphatidylserine magnetic-activated cell separation spermatozoa

Modeling and Analysis of Low Frequency Noise in Ion-Field-Effect Transistors Sensors

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Modeling and Numerical Simulation of Material Science 2014年第3期4卷 119-127页

作者： Jihen Chermiti Sawsen Azzouzi Mounir Ben Ali Mhamed Trabelsi Abdelhamid Errachid Higher Institute of Applied Sciences and Technology of Sousse University of Sousse Sousse Tunisia Higher Institute of Applied Sciences and Technology of Sousse University of Sousse Sousse Tunisia Laborory Materials Molecules and Applications University of Carthage IPEST Tunis Tunisia Institute of Analytical Sciences of Lyon Université Claude Bernard Lyon 1 Lyon France

Ions Sensitive Field Effect Transistors (ISFETs) are becoming the platform sensors for important chemical and biomedical applications. However, the accuracy of ISFET output measurement is greatly affected by the presences of low-frequency noise, drift and slow response of the device. This requires more safety in measured results and the tools of analysis. In this paper, we present fundamental limits on the sensitivity of ISFETs micro-sensors, arising from intrinsic and extrinsic noise sources. We developed an algorithm in MATLAB in order to model the frequency analysis of the 1/f noise in ISFET sensor using Hooge theory. We have shown that the 1/f noise of the ISFETs sensors is due to both the electrochemical system (pH solution) and the MOS component (canal size, insulator thickness). The temperature effect on the ISFET noise and the signal conditioning are also performed.

关键词： Top-Spice Modeling Ion Sensitive Field Effect Transistor Low-Frequency Noise

Study of gonadic growth factors: Seminal transforming growth factor-β1, epidermal growth factor and insulin-like growth factor-I and their relationship with male infertility

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Advances in Reproductive Sciences 2014年第1期2卷 16-23页

作者： Houda Amor mounir Ajina ali Saad Habib ben ali Laboratoire de Cytogénétique Génétique Moléculaire et Biologie de la Reproduction Humaines CHU Farhat Hached Sousse Tunisie Unité de Médecine de la Reproduction CHU Farhat Hached Sousse Tunisie

Background: The aim of this study was to establish normal reference values for several growth factors in semen and provide a basis for understanding their physiological significance in fertile and infertile subjects. Methods: 110 infertile men semen was evaluated according to WHO standards and by enzyme immunoassay (ELISA kit protocol) to determine the rate of growth factors (TGFβ1, IGFI, EGF). We have also listed neutrophils by staining with peroxidase to examine the association of inflammation with the levels of these factors. The determination of the epididymal (alpha-1,4-glucosidase) marker was directed according to the technical recommended by the WHO in 2010. Each patient received a hormonal balance including the determination of plasma FSH, LH and Testosterone. Results: Concentrations of TGFβ1 were significantly higher in (severe and extreme azoospermic, oligospermic) pathological groups compared to the control group (P 0.05) between control and pathological groups. The IGFI is partly testicular and/or epididymal as it is lowered in some patients with obstructive azoospermia. Positive correlations were observed between the seminal IGFI, mobility (r = 0.487) and sperm morphology (r = +0.187). Immunological sperm abnormalities and/or idiopathic do not alter the rate of EGF seminal. There is no significant correlation between seminal EGF or with sperm parameters or with plasma levels of FSH and LH. Conclusion: The growth factors are primarily peptides, handling and identification in a complex environment as the seminal plasma, but it requires a lot of precautions if we do not want to expose to the risk of bad farms/interpretations of data obtained.

关键词： Infertility Semen EGF IGF-I TGF-β1 FSH LH

CRP Binding Kinetics Enhancement Using Local Narrowing into a bent Channel: Finite Element Analysis

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Engineering（科研） 2022年第1期14卷 62-75页

作者： Mohamed Bahri Imen Dermoul Marshet Getaye Mohamed Amin Elaguech Khouloud Djebbi Mounir Ben Ali Chaker Tlili Deqiang Wang Chongqing School University of Chinese Academy of Sciences Chongqing China Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences Chongqing China University of Chinese Academy of Sciences Beijing China Preparatory Institute for Engineering of Nabeul IPEIN University of Carthage Campus Universitaire Merazka Tunisia NANOMISENE Lab LR16CRMN01 Centre for Research on Microelectronics and Nanotechnology of Sousse Technopole of Sousse Sousse Tunisia University of Sousse Higher Institute of Applied Sciences and Technology of Sousse GREENS-ISSAT Cité Ettafala Sousse Tunisia

Binding kinetics enhancement of a microfluidic biosensor into a micro-channel through the application of a supplementary mechanism has received tremendous attention because of the obtained significant enhancement factor. However, biosensor’s performance enhancement using only simple channel engineering is still rarely realized. Herein, we present a novel design of a complex reactive protein (CRP) biosensor into a U-shaped channel with a sensitive membrane located in the middle of the bent zone. Various critical factors affecting the equilibrium binding time are numerically investigated. The turn geometry is then optimized when the arc length along the inner and outer radii is almost the same, which leads to locally minimizing the channel height overhead the reaction surface and improves the analyte transport towards the sensing area. The numerical studies reveal that applying a local narrowing above the reaction surface can notably enhance the trapping and the surface formation of complex antibody-antigen, thus upgrading the biosensor performance. This work puts a significant advance towards microfluidic channel engineering and the exploration of micro-flow injection experimental studies.

关键词： Finite Elements Method Binding Reaction CRP Biosensor Microfluidic System Microchannel Turn Numerical Simulation

Enhanced photocatalytic activities of silicon nanowires/graphene oxide nanocomposite:Effect of etching parameters

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Journal of Environmental Sciences 2021年第3期33卷 123-134页

作者： mounir Gaidi Kais Daoudi Soumya Columbus Anouar Hajjaji My ali El Khakani Brahim Bessais Department of Applied Physics and Astronomy University of SharjahSharjah 27272United Arab Emirates Sharjah Centre for Advanced Materials Research Research Institute of Sciences and EngineeringUniversity of SharjahSharjah 27272United Arab Emirates Sharjah Sharjah Research Academy University CitySharjah 60999United Arab Emirates Sharjah Laboratoire de Photovoltaïque Centre de Recherches et des Technologies de l’EnergieTechnopole de Borj-CédriaHammam-Lif 2050Tunisia Institut National de la Recherche Scientifique(INRS) INRS-ÉnergieMatériaux et Télécommunications1650Blvd.Lionel-BouletVarennesQC J3X-1S2Canada

Homogeneous and vertically aligned silicon nanowires(SiNWs)were successfully fabricated using silver assisted chemical etching technique.The prepared samples were characterized using scanning electron microscopy,transmission electron microscopy and atomic force microscopy.Photocatalytic degradation properties of graphene oxide(GO)modified SiNWs have been investigated.We found that the SiNWs morphology depends on etching time and etchant composition.The SiNWs length could be tuned from 1 to 42μm,respectively when varying the etching time from 5 to 30 min.The etchant concentration was found to accelerate the etching process;doubling the concentrations increases the length of the SiNWs by a factor of two for fixed etching time.Changes in bundle morphology were also studied as function of etching parameters.The SiNWs diameter was found to be independent of etching time or etchant composition while the size of the SiNWs bundle increases with increasing etching time and etchant concentration.The addition of GO was found to improve significantly the photocatalytic activity of SiNWs.A strong correlation between etching parameters and photocatalysis efficiency has been observed,mainly for SiNWs prepared at optimum etching time and etchant concentrations of 10 min and 4:1:8.A degradation of92%was obtained which further improved to 96%by addition of hydrogen peroxide.Only degradation efficiency of 16%and 31%has been observed for bare Si and GO/bare Si samples respectively.The obtained results demonstrate that the developed SiNWs/GO composite exhibits excellent photocatalytic performance and could be used as potential platform for the degradation of organic pollutants.

关键词： Photocatalysis Nanomaterials Methylene Blue Silicon nanowire Graphene oxide Water treatment

beneficial Effect of an Oral Antioxidant Supplementation (Fertimax2) on IVF-ICSI Outcomes: A Preliminary Clinical Study

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Advances in Reproductive Sciences 2014年第3期2卷 47-56页

作者： Olfa Kacem Meriem Harzallah Chekib Zedini Ines Zidi Sawsen Meddeb Meriem Fékih Habib Saidi Anouar Chaib Sassi Boughizane Habib ben ali Mohamad Bibi ali Saad Hédi Khairi mounir Ajina Department of Obstetrics and Gynaecology University Hospital F. Hached Sousse Tunisia Laboratories of Cytogenetic Molecular Biology and Human Biology of Reproduction F Hached Hospital Sousse Tunisia Service of Community Medicine Faculty of Medicine Sousse Tunisia Unit of Reproductive Medicine University Hospital F Hached Sousse Tunisia

Background: Considerable evidence points towards a significant role of oxidative stress (OS) in the pathogenesis of sperm dysfunction. OS as a result of an inappropriate balance between oxidants and antioxidants in the semen can cause DNA damage and lipid peroxidation leading to failure of conception, miscarriage or potentially even childhood cancer. The objective of this study was to investigate whether a male antioxidant therapy can improve semen parameters and the results of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Methods: A total of forty eight infertile couples were considered. Male participants were administrated Fertimax2 antioxidant treatment for at least two months prior to their partner’s IVF-ICSI cycle. Sperm parameters (volume, concentration, progressive motility) and the IVF-ICSI outcomes were compared before and after the antioxidant treatment. The primary outcome measures were oocyte fertilization, cleavage and good embryo quality rates;the secondary outcomes were biochemical pregnancies, clinical pregnancies and implantation rates. Results: The principal finding that emerged from this study was that antioxidant therapy resulted in significant improvements in fertilization (p = 00.2), cleavage (p = 0.004) and good-embryo quality (p = 0.002) rates accompanied by a marked increase in clinical pregnancy (18.7% versus 2.5%) and implantation (11.8% versus 1.02%) rates. No significant changes in routine sperm parameters were observed. Conclusion: The Fertimax2 antioxidant therapy appears to influence favorably chances of conception in couples undergoing assisted reproduction treatment (ART).

关键词： Sperm Oxidative Stress Antioxidants Infertility Treatment IVF-ICS

Impact of the professional exposure on the spermatic parameters and the results of ICSI at Tunisian unfertile couples

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Advances in Reproductive Sciences 2014年第1期2卷 24-31页

作者： Habib ben ali Monia Zaouali Fatma Atig Iheb Bougmiza Souhir Mehri Houda ben Frej Faten Tabka ali Saad mounir Ajina Laboratoire de Cytogénétique Génétique Moléculaire et Biologie de la Reproduction Humaines CHU Farhat Hached de Sousse Sousse Tunisie Laboratoire de Physiologie et des Explorations Fonctionnelles Faculté de Médecine de Sousse Sousse Tunisie Service de Médecine Communautaire Faculté de Médecine de Sousse Sousse Tunisie Service de Médecine de Travail CHU Farhat Hached de Sousse Sousse Tunisie Unité de Médecine de la Reproduction CHU Farhat Hached de Sousse Sousse Tunisie

Introduction: The association of the professional exposure to the decline of the male reproductive function had been the subject of several studies but without arriving to one envisagement undeniable of its existence. The ICSI, technique of reference in severe male infertilities, is visavis unexplained failures whose environmental and professional exposure could be about its cause partly. In this work, we tried to highlight an association between certain factors of professional exposure and the deterioration of the spermatic parameters, then to evaluate the effect of such factors on the result of the ICSI. Materials and Methods: It is about a study of the exposed type—no exposed, realized through collaboration between the Unit of Medicine of the Reproduction and the Service of Medicine of Work of the CHU Farhat Hached of Sousse. This study was based on a questionnaire of which part of the items was collected medical files of the patients raising the general characteristics, the profile of exposure as well as the spermatic parameters and the biological assessment of ICSI. Results: Significant associations between the deterioration of sperm and the professional risk factors such as the exposure to the formaldehydes (p = 0.01) and heat (p = 0.001) were noted. The exposure to formaldehydes was associated at the higher risk of oli gozoospermy, of asthenozoospermy with a significant reduction in mobility (p = 0.05), whereas the talks with cement had a risk higher of teratozoospermy. In addition an exposure to heat was associated at the high risk of oligoteratozoospermy. The total rate of pregnancies after ICSI among 220 patients of our series was of 14.47%. The ICSI balance showed a reduction in total fertility rate (76.6% exposed vs 75.3% no exposed). Conclusion: In spite of the limited number of the patients, this study had shown a correlation between certain professional exposures of origin and the deterioration of sperm. They are preliminary results which encourage continuing collaboration between the specialists i

关键词： Male Sterility Professional Exposure Spermogramm ICSI

Characterization of Tunisian marine sediments in Rades and Gabes harbors

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International Journal of Sediment Research 2014年第3期29卷 391-401页

作者： Imen BEL HADJ ali Zoubeir LAFHAJ mounir BOUASSIDA Imen SAID Ecole Centrale de Lille Villeneuve d'AscqFrance and Ecole Nationale d'Ingenieurs de TunisTunisia Ecole centrale de Lille Villeneuve d'AscqFrance Universite de Tunis El Manar/Ecole Nationale d'Ingenieurs de Tunis/05-UR-11 06 Universite de Tunis El Manar/Ecole Nationale d'Ingenieurs de Tunis/05-UR-1106

The objective of this article is to study the geotechnical and environmental characteristics of sediments dredged from two Tunisian harbors:Rades and Gabes.The first harbor represents the main facility place in the national transport chain.The second one is selected as its sediments present a serious ecological constraint caused by the discharge of wastes into the marine environment.These sediments are either discarded at sea or landfilled despite their harmful effects on the environment.The article is divided into three main sections.The first one presents the material that was carried for Rades and Gabes harbors.The conservation conditions and the used experimental tests are detailed.Geotechnical characterization includes the determination of the grain size distribution,the water content,the Atterberg limits,the methylene blue value,the specific area,the bulk density,the specific unit weight,the organic and carbonate contents.Environmental characterization is assessed by the determination of metals concentrations in a leaching solution.The second section deals with the description and analysis of geotechnical properties of Rades and Gabes harbors’ sediments.The results obtained show that Rades harbor sediments are slightly sandy clayey silts whereas Gabes harbor sediments are silty sands characterized by a highly plastic clay fraction.Both of the two sediments don’t exhibit a high organic content.Finally,chemical,mineralogical and environmental properties are presented and then analysed.The experimental results obtained show that Rades and Gabes sediments could be used as a sand substitute in the formulation of a new construction material.Gabes harbor sediments are more polluted than Rades harbor sediments.

关键词： Sediments Characterization Geotechnical Environmental Valorization

Survey on Segmentation and Classification Techniques of Satellite Images by Deep Learning Algorithm

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Computers, Materials & Continua 2023年第6期75卷 4973-4984页

作者： Atheer Joudah Souheyl Mallat mounir Zrigui Department of Computer Sciences University of MonastirMonastir1001Tunisia Research Laboratory in Algebra Numbers Theory and Intelligent SystemsMonastir1001Tunisia

This survey paper aims to show methods to analyze and classify field satellite images using deep learning and machine learning algorithms.Users of deep learning-based Convolutional Neural Network(CNN)technology to harvest fields from satellite images or generate zones of interest were among the planned application scenarios(ROI).Using machine learning,the satellite image is placed on the input image,segmented,and then tagged.In contem-porary categorization,field size ratio,Local Binary Pattern(LBP)histograms,and color data are taken into account.Field satellite image localization has several practical applications,including pest management,scene analysis,and field tracking.The relationship between satellite images in a specific area,or contextual information,is essential to comprehending the field in its whole.

关键词： Identification satellite images classify deep learning machine learning