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hVEGF165 Expression in Escherichia coli Conserves Its Biological Function

Journal of Chemistry and Chemical Engineering 2012年第8期6卷 738-743页

作者： Gabajova Blanka Valkova Danka Bohac Andrej Kovacova Elena moravcik roman Zeman Michal Department of Organic Chemistry FNS Comenius University Bratislava 84215 Slovak Republic Department of Animal Physiology and Ethology FNS Comenius University Bratislava 84215 Slovak Republic

The paper describes the expression of human protein VEGF165 in Escherichia coli and its purification. This growth factor isoform contains exon 7, which is essential for binding to extracellular domain of VEGF receptor 2, located on endothelial cells lining the surface of blood vessels. This binding stimulates the cascade of downstream signalling events leading to process known as angiogenesis, hVEGF165 overexpressed with His-tag in BL21 E. coli cells forms inclusion bodies （insoluble protein）, so the research found the procedure for its solubilization and purification on a Nickel based affinity chromatography. Although this eukaryotic signal protein needs posttranslational processing for its full function as a homodimer, author verified the biological activity of our hVEGF165 protein, obtained as monomer, by wound healing test.

关键词： VEGFI65 endothelial cells hypoxia angiogenesis inclusion bodies protein purification wound healing test.

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The Proposal of Data Warehouse Validation

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Computer Technology and Application 2011年第8期2卷 650-657页

作者： Pavol Tanuska Michal Kebisek Oliver moravcik Pavel Vazan Institute of Applied lnJormatics Automation and Mathematics Faculty of Materials Science and Technology Slovak University of Technology Trnava 91724 Slovakia

The analysis of relevant standards and guidelines proved the lack of information on actions and activities concerning data warehouse testing. The absence of the complex data warehouse testing methodology seems to be crucial particularly in the phase of the data warehouse implementation. The aim of this article is to suggest basic data warehouse testing activities as a final part of data warehouse testing methodology. The testing activities that must be implemented in the process of the data warehouse testing can be split into four logical units regarding the multidimensional database testing, data pump testing, metadata and OLAP （Online Analytical Processing） testing. Between main testing activities can be included： revision of the multidimensional database scheme, optimizing of fact tables number, problem of data explosion, testing for correctness of aggregation and summation of data etc.

关键词： Data warehouse test case testing activities methodology validation UML (unified modeling language).

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