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Nonmuscle myosin Ⅱ regulates migration but not contraction in rat hepatic stellate cells

World Journal of Hepatology 2011年第7期3卷 184-197页

作者： Cathy C moore Ashley M Lakner Christopher M Yengo Laura W Schrum Department of Biology University of North Carolina at CharlotteCharlotteNC 28223United States Liver Digestive and Metabolic Disorders LaboratoryCarolinas Medical Center

AIM: To identify and characterize the function of non-mu-scle myosin Ⅱ (NMM Ⅱ) isoforms in primary rat hepatic stellate cells (HSCs).METHODS: Primary HSCs were isolated from male Spra-gue-Dawley rats by pronase/collagenase digestion. Total RNA and protein were harvested from quiescent and culture-activated HSCs. NMM Ⅱ isoform (Ⅱ-A, Ⅱ-B and Ⅱ-C) gene and protein expression were measured by RealTime polymerase chain reaction and Western blot analyses respectively. NMM Ⅱ protein localization was visualized in vitro using immunocytochemical analysis. For in vivo assessment, liver tissue was harvested from bile duct-ligated (BDL) rats and NMM Ⅱisoform expression determined by immunohistochemistry. Using a selective myosin Ⅱ inhibitor and siRNA-mediated knockdown of each isoform, NMM Ⅱ functionality inprimary rat HSCs was determined by contraction and migration ***: NMM Ⅱ-A and Ⅱ-B mRNA expression was increased in culture-activated HSCs (Day 14) with sig-niflicant increases seen in all pairwise comparisons (Ⅱ-A: 12.67 ± 0.99 (quiescent) vs 17.36 ± 0.78 (Day 14), P < 0.05; Ⅱ-B: 4.94 ± 0.62 (quiescent) vs 13.90 ±0.85 (Day 14), P < 0.001). Protein expression exhibited similar expression patterns (Ⅱ-A: 1.87 ± 2.50 (quiescent) vs 58.64 ± 8.76 (Day 14), P < 0.05; Ⅱ-B: 1.17 ± 1.93 (quiescent) vs 103.71 ± 21.73 (Day 14), P < 0.05). No signif icant differences were observed in NMM Ⅱ-C mRNA and protein expression between quiescent and activated HSCs. In culture-activated HSCs, NMM Ⅱ-A and Ⅱ-B merged with F-actin at the cellular periphery and throughout cytoplasm respectively. In vitro stud-ies showed increased expression of NMM Ⅱ-B in HSCs activated by BDL compared to sham-operated animals. There were no apparent increases of NMM Ⅱ-A and Ⅱ-C protein expression in HSCs during hepatic BDL injury. To determine the contribution of NMM Ⅱ-A and Ⅱ-B to migration and contraction, NMM Ⅱ-A and Ⅱ-B expres-sion were downregulated with siRNA. NMM Ⅱ-A and/or Ⅱ-B siRNA inhibited HSC mi

关键词： Hepatic stellate cells Nonmuscle myosin Ⅱ Migration Contraction Blebbistatin Hepatic injury

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Developing a genome-wide selection model for genetic improvement of residual feed intake and carcass merit in a beef cattle breeding program

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Chinese Science Bulletin 2012年第21期57卷 2741-2746页

作者： ZENG ZhiYao TANG Guo Qing MA JiDeng PLASTOW Graham moore Stephen LAI SongJia LI XueWei WANG ZhiQuan College of Animal Science and Technology Sichuan Agricultural UniversityYaan 625014China Department of Agricultural Food and Nutritional ScienceUniversity of AlbertaEdmontonAB T6G 2C8Canada College of Animal Science and Technology Heilongjiang August First Land Reclamation UniversityDaqing 163319China

Residual feed intake (RFI) and carcass merit (CM) are both complex traits emerging as critical targets for beef genetic *** and CM traits are difficult and expensive to measure and genetic improvement for these traits through traditional selection methods is not very ***,genome-wide selection using DNA markers may be a potential alternative for genetic improvement of these *** this study,the efficiency of a genome-wide selection model for genetic improvement of RFI and CM was *** Illumina Bovine50K bead chip was used to genotype 922 beef cattle from the Kinsella Beef Research Ranch of the University of Alberta.A Bayes model and multiple marker regression using a stepwise method were used to conduct the association *** number of significant SNP markers for carcass weight (CWT),carcass back fat (BF),carcass rib eye area (REA),carcass grade fat (GDF),lean meat yield (LMY),and residual feed intake (RFI) were 75,54,67,57,44 and 50,***-variate analysis of marker scores and phenotypes for all traits were made using DMU *** genetic parameter for each trait was *** genetic correlations of marker score and phenotype for CWT,BF,REA,GDF,LMY and RFI were 0.75,0.69,0.87,0.77,0.78,and 0.85,*** average prediction accuracies of phenotypic EBV for the six traits were increased by 0.05,0.16,0.24,0.23,0.17 and 0.19,*** results of this study indicated that the two-trait marker-assisted evaluation model used was a suitable alternative of genetic evaluation for these traits in beef cattle.

关键词：胴体品质遗传改良全基因组选择模型采食量育种计划肉牛剩余

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China＇s ＂Peaceful Rise＂： Rhetoric or Reality7

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Fudan Journal of the Humanities and Social Sciences 2010年第4期3卷 132-137页

作者： Gregory J. moore

With the astonishing growth in China＇s economy over the past thirty years and the concomitant growth in China＇s power and influence in international affairs have come a number of new ＂China＇s rise＂ books, for very understandable reasons. Among the more notable examples are those by Taylor Fravel, David Kang and David （Mike） Lampton,

关键词：中国经济发展发展战略市场分析

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Realism on China＇s Rise： The Limits of Harmony （ and War）

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Fudan Journal of the Humanities and Social Sciences 2013年第2期6卷 143-150页

作者： Gregory J. moore

Wang Yuan-kang has written an important book on China＇s historical foreign policy and the implications of these historical arguments on China＇s present day policies. Titled, Harmony and War： Confucian Culture and Chinese Power Politics （Columbia University Press, 2010）, Wang＇s volume compares two approaches to understanding China＇s historical foreign policy： Confucian pacifism, and structural Realism;

关键词：中国历史现实主义战争和谐哥伦比亚大学外交政策儒家文化出版社

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Dental Anxiety among Danish Adults—Comparison of Recent Website Data and Older Telephone Data with Government Demographic Statistics

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Open Journal of Stomatology 2017年第12期7卷 530-544页

作者： Rod moore Peter Bering Dental Anxiety Research & Treatment Center Department of Dentistry Aarhus University Aarhus Denmark Department of Public Health Sciences Aarhus University Aarhus Denmark

Background: Survey quality, in particular sampling, coverage, and issues of representativity, are important for valid and reliable conclusions from epidemiological data. Dental anxiety (DA) still challenges dental clinicians since it is synonymous with care avoidance. Accurate estimates of DA are important for public health. Aims were to 1) assess demographic representativity (age/ gender) of a 2013-14 web survey and a 1992-93 telephone survey about DA in Danish adults aged 16 - 80 yr using government statistics;2) assess DA frequency and characteristics from web survey data (N = 701);and 3) compare web results with 1993 results. Method: Dental Anxiety Scale (DAS) measured DA, while other items revealed gender, age, education, dentist avoidance, and three types of negative dentist behaviors. Analyses used frequencies, Chi- square, odds ratios (OR) and ANOVAs. Results: Samples from 1992-3 and 2013-14 were not significantly different by demographics or government statistics, with the exception of low numbers in ages 16 - 19 yr for both surveys. Ages 20 - 29 yr and 30 - 39 yr were slightly overrepresented in telephone data, while ages 50 - 59 yo were in web data. Mean DAS scores were 7.5 for both 1992-3 and 2013-14. Extreme DA (DAS 20-15) increased from 4.2% to 5.3%, while high DA (DAS ≥ 13) increased from 6% to 9.5%. Main 2013-14 associations with DAS ≥ 15 were women (OR = 4.7), avoiding dentists (OR = 11.4) and negative dentist behaviors (OR = 4.2 - 6.7) similar to 1992-3 data. Conclusion: Web survey results from this convenience sample were demographically representative for adults 20 - 80 yo and showed small changes in DA after 20 years. Future strategies regarding survey of teenagers require special attention.

关键词： Dental Anxiety Prevalence Characteristics Epidemiological Methods Subject Recruitment Web Surveys Telephone Surveys Demographic Representativity

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A POINTER NAMED CHEYENNE

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英语自学 2000年第12期 9-13页

作者： Catherine moore 王京

我父亲朝我嚷着:“当心!你差一点撞到那部汽车的侧面去了。你就不能稳当点儿来做事?”他说这些话使我感到比挨打还要难受。我转过头去瞧一眼那个坐在我邻座的老年人,这使我有勇气与他对抗。当我把视线移开时,感到有个肿块在我的喉管里... 详细信息

我父亲朝我嚷着:“当心!你差一点撞到那部汽车的侧面去了。你就不能稳当点儿来做事?”他说这些话使我感到比挨打还要难受。我转过头去瞧一眼那个坐在我邻座的老年人,这使我有勇气与他对抗。当我把视线移开时,感到有个肿块在我的喉管里哽着。我没作好再次与他争吵的准备。

关键词： A POINTER NAMED CHEYENNE

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A Comparative Analysis of Three English Translations of Liu Xie’s Wenxin Diaolong:Dragon-Carving and the Literary Mind,The Book of Literary Design,and The Literary Mind and the Carving of Dragons

A Comparative Analysis of Three English Translations of Liu ...

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作者： Aaron Lee moore School of Literature and Journalism Sichuan University

This essay is the first study to undertake an analysis of the only three complete English editions of the monumental work of medieval Chinese literary theory,Wenxin Diaolong,through close reading and sensitive *** essay mainly compares the varying translations of chapter titles and the scholarly apparati of the *** the conclusion of the essay,a recommendation is offered as to which edition would be best suited to prospective foreign English-speaking Dragonologists,thosewho study Wenxin Diaolong.

关键词： Dragon Carving Literary Mind Wenxin Diaolong Liu Xie Translation Dragonology

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Faulkner’s Reception in France and the Influence of Existentialism

Faulkner’s Reception in France and the Influence of Existen...

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作者： Aaron Lee moore School of Literature and Journalism Sichuan University Virginia

This essay argues that Faulkner’s positive reception in France was largely due to the predominance of existential themes in his works,which in turn garnered the early attention of modernist French scholars,writers,and *** French existential philosopher Jean-Paul Sartre,though critical of Faulkner’s metaphysic of time in The Sound and the Fury,still found much in Faulkner’s writing worthy of *** essay surveys existential criticism on Faulkner’s works,offers an explanation for its recent neglect in scholarship,and suggests that existentialphenomenological criticism is still highly applicable as a critical theoretical framework in which to approach the Faulkner canon.

关键词： Faulkner Sartre France existentialism phenomenology

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Liquid deposition photolithography for submicrometer resolution three-dimensional index structuring with large throughput

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Light(Science & Applications) 2013年第1期2卷 209-214页

作者： Adam C Urness Eric D moore Keith K Kamysiak Michael C Cole Robert R McLeod Department of Electrical Engineering University of Colorado at BoulderBoulderCO 80309USA.

Photonic devices increasingly require three-dimensional control of refractive index,but existing fabrication methods such as femtosecond micromachining,multilayer lithography and bulk diffusion can only address a select scale range,are often limited in complexity or thickness and have low *** introduce a new fabrication method and polymeric material that can efficiently create mm^(3) optical devices with programmable,gradient index of refraction with arbitrary feature *** contrast of 0.1 is demonstrated,which is 100 times larger than femtosecond micromachining,and 20 times larger than commercial holographic *** is achieved by repetitive microfluidic layering of a self-developing photopolymer structured by projection *** process has the unusual property that total fabrication time for a fixed thickness decreases with the number of layers,enabling fabrication 10^(5) faster than femtosecond *** demonstrate the process by sequentially writing 100 layers to fabricate a mm thick waveguide array.

关键词： holographic photopolymers lithography photonic device fabrication single photon polymerization three-dimensional structuring

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Rural nurses’ perceptions of a volunteer program in an acute setting: Volunteers delivering person-centred care for patients with dementia and delirium

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Open Journal of Nursing 2014年第1期4卷 27-33页

作者： Kaye Ervin Sharon moore Centre for Nursing & Health Studies Athabasca University Alberta Canada Rural Health Academic Centre University of Melbourne Shepparton Australia

Community volunteers were recruited and trained to deliver person-centred care to patients with dementia or delirium in an acute hospital setting, in a small rural Australian hospital. The volunteer program was grounded in action research methodology, and modelled on a previous research project. As a form of evaluation, interviews were conducted with nursing staff eight weeks after implementation of the volunteer program to explore their opinions. Data were analysed through a collaborative process and findings revealed strong benefits from the perspectives of the nursing staff. These benefits included overall improved patient care and improved time management for nursing tasks.

关键词： Acute Setting Dementia Person-Centred Care Volunteers

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