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Submergence promotes auxin-induced callus formation through ethylene-mediated post-transcriptional control of auxin receptors

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Molecular Plant 2022年第12期15卷 1947-1961页

作者： Seung Yong Shin Yuri Choi Sang-Gyu kim Su-Jin Park Ji-Sun Park Ki-Beom moon Hyun-Soon kim Jae Heung Jeon Hye Sun Cho Hyo-jun Lee Plant Systems Engineering Research Center Korea Research Institute of Bioscience and BiotechnologyDaejeon 34141Korea Department of Functional Genomics KRIBB School of BioscienceUniversity of Science and TechnologyDaejeon 34113Korea Department of Biological Sciences Korea Advanced Institute of Science and TechnologyDaejeon 34141Korea Department of Biosystems and Bioengineering KRIBB School of BiotechnologyUniversity of Science and TechnologyDaejeon 34113Korea Department of Biological Sciences Sungkyunkwan UniversitySuwon 16419Korea

Plant cells in damaged tissue can be reprogrammed to acquire pluripotency and induce callus ***,in the aboveground organs of many species,somatic cells that are distal to the wound site become less sensitive to auxin-induced callus formation,suggesting the existence of repressive regulatory mechanisms that are largely *** we reveal that submergence-induced ethylene signals promote callus formation by releasing post-transcriptional silencing of auxin receptor transcripts in non-wounded *** determined that short-term submergence of intact seedlings induces auxin-mediated cell dedifferentiation across the entirety of Arabidopsis thaliana *** constitutive triple response 1-1(ctr1-1)mutation induced callus formation in explants without submergence,suggesting that ethylene facilitates cell *** show that ETHYLENE-INSENSITIVE 2(EIN2)post-transcriptionally regulates the abundance of transcripts for auxin receptor genes by facilitating microRNA393 ***-induced calli in non-wounded regions were suitable for shoot regeneration,similar to those near the wound *** also observed submergence-promoted callus formation in Chinese cabbage(Brassica rapa),indicating that this may be a conserved mechanism in other *** study identifies previously unknown regulatory mechanisms by which ethylene promotes cell dedifferentiation and provides a new approach for boosting callus induction efficiency in shoot explants.

关键词： cell dedifferentiation auxin signaling ethylene callus formation EIN2

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Bird-mimetic Wing System of Flapping-wing Micro Air Vehicle with Autonomous Flight Control Capability

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Journal of Bionic Engineering 2016年第3期13卷 458-467页

作者： Sriyulianti Widhiarini Ji Hwan Park Bum Soo Yoon Kwang Joon Yoon ll-Hyun Paik Jong Heon kim Chan Yik Park Seung moon jun Changho Nam Department of Aerospace Engineering Konkuk University Gwangjin-gu Seoul South Korea Deptartment of PGM Technology Hanwha Corp. R&D Center Daejeon South Korea Agency for Defense Development Daejeon South Korea Department of Engineering Technology Arizona State University Mesa Arizona

A micro air vehicle with a bird-mimetic up-down and twisting wing drive system was developed in this study. The Flap- ping-wing Micro Air Vehicle （FMAV）, with a 50 cm wingspan and a double-crank drive system, performed successful flights of up to 23 min. The performance and capabilities of the FMAV were enhanced by adapting a number of unique features, such as a bird-mimetic wing shape with a span-wise camber and an up-down and twisting wing drive mechanism with double-crank linkages, This lift-enhancing design by mimicking the flapping mechanism of a bird＇s wing enabled the 210 g FMAV to fly autonomously in an outdoor field under wind speeds of less than 5 m.s-1. Autonomous flight was enabled by installing a flight control computer with a micro-electro-mechanical gyroscope and accelerometers, along with a micro video camera and an ultralight wireless communication system inside the fuselage. A comprehensive wind tunnel test shows that the FMAV with a high-camber wing and double-crank mechanism generates more lift and less net thrust than the FMAV with a flat wing and single-crank mechanism, which confirms the improved performance of the developed FMAV, as well as the superior slow flying or hovering capabilities of the FMAV with a high-camber wing and double-crank wing drive system.

关键词： flapping-wing MAV autonomous flight biomimetic wing double crank wing

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Diagnostic performance of the current risk-stratified approach with computed tomography for suspected choledocholithiasis and its options when negative finding

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Hepatobiliary & Pancreatic Diseases International 2019年第4期18卷 366-372页

作者： Hyun Woo Lee Tae jun Song Do Hyun Park Sang Soo Lee Dong-Wan Seo Sung Koo Lee Myung-Hwan kim Jae Hyuck jun Ji Eun moon Yeon Han Song Digestive Disease Center and Research Institute Department of Internal MedicineSoonchunhyang University School of MedicineBucheonKorea Division of Gastroenterology Department of Internal MedicineUniversity of Ulsan College of MedicineAsan Medical CenterSeoulKorea Division of Gastroenterology Department of Internal MedicineUniversity of Eulji College of MedicineEulji Medical CenterDaejeonKorea Department of Biostatistics Clinical trial centerSoonchunhyang University Bucheon HospitalBucheonKorea

Background:Several studies evaluated the current guideline of the American Society for Gastrointestinal Endoscopy(ASGE)and reported only suboptimal *** study evaluated the diagnostic performance of the ASGE guideline based on computed tomography(CT)and role of endoscopic ultrasonography(EUS)and magnetic resonance cholangiopancreatography(MRCP)in patients with suspected choledocholithiasis but negative CT ***:Patients with suspected choledocholithiasis undergoing ERCP between January 2016 and January 2017 were retrospectively *** patients underwent CT to detect *** or MRCP was performed when the CT scan showed negative *** were classified into the high and intermediate-risk groups,based on predictors from the ASGE ***:Of 583 patients with suspected choledocholithiasis,340(58.3%)had stones on ERCP(65.9%in the high-risk group and 40.6%in the intermediate-risk group).The accuracy of ASGE guideline for CT was 63.98%(79.12%sensitivity,42.80%specificity)and 36.02%(20.88%sensitivity,57.20%specificity)in the high-risk and intermediate-risk groups,*** 103 patients in the high-risk group underwent both CT and US,the accuracy of CT was higher than that of US for detecting choledocholithiasis(78.64%vs.53.40%),with a significant difference in area under the curve(AUC)(0.78 vs.0.59,P<0.001).Of 339 with negative CT finding,the accuracy of EUS was higher than that of MRCP(90.91%vs.82.76%),but with no significant difference in AUC(0.91 vs.0.83,P=0.347).Conclusions:CT-based ASGE guideline showed superior diagnostic performance than US for predicting *** diagnostic options,EUS or MRCP,with negative CT finding showed comparable ***,the diagnostic modality should be selected based on availability,experience,cost,and contraindications.

关键词： Choledocholithiasis ASGE guideline Accuracy Endoscopic ultrasound Magnetic resonance cholangiopancreatography

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Two-step generation of induced pluripotent stem cells from mouse fibroblasts using Id3 and Oct4

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Journal of Molecular Cell Biology 2012年第1期4卷 59-62页

作者： Jai-Hee moon june Seok Heo Suhyun Kwon Jihyun kim Jihye Hwang Phil jun Kang Aeree kim Hyun Ok kim Kwang Youn Whang Byung Sun Yoon Seungkwon You Laboratory of Cell Function Regulation College of Life Sciences and BiotechnologyKorea UniversitySeoulRepublic of Korea Cell Therapy Center College of MedicineYonsei UniversitySeoulRepublic of Korea Department of Pathology College of MedicineKorea University Guro HospitalSeoulRepublic of Korea Division of Biotechnology College of Life Sciences and BiotechnologyKorea UniversitySeoulRepublic of Korea

Dear Editor,Somatic cells can be reprogrammed into pluripotent stem cells,called induced pluripotent stem cells(iPSCs),by defined transcription factors(Takahashi et al.,2006).The reprogramming of somatic cells may be a continuous stochastic process in which nearly all somatic donor cells have the ability to give rise to iPSCs with continuous passaging and the expression of defined factors(Hanna et al.,2009).However,the reprogramming process can be divided into discrete stages,including the generation of dedifferentiated pre-pluripotent cells followed by authentic pluripotent cells(Silva et al.,2009).

关键词： pluripotent programming

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Development of large acceptance multi-purpose spectrometer in Korea for symmetry energy

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Nuclear Science and Techniques 2018年第12期29卷 6-14页

作者： Byungsik Hong jung Keun Ahn Gyeonghwan Bak Jamin Jo Minho kim Eun Joo kim Young Jin kim Young jun kim Minjung Kweon Hanseul Lee Hyo Sang Lee Jong-Won Lee jung Woo Lee Kyong Sei Lee Byul moon Dong Ho moon Benard Mulilo Jaebeom Park Min Sang Ryu Hyunha Shim LAMPS Collaboration Department of Physics Korea University Seoul 02841Republic of Korea Department of Physics Chonnam National UniversityGwangju 61186 Republic of Korea Division of Science Education Chonbuk National UniversityJeonju 54896 Republic of Korea Rare Isotope Science Project Institute for Basic ScienceDaejeon 34047 Republic of Korea Department of Physics Inha University Incheon 22212Republic of Korea

The Rare Isotope Accelerator complex for ONline experiments(RAON) is a new radioactive ion beam accelerator facility under construction in Korea. The large acceptance multi-purpose spectrometer(LAMPS) is one of the experimental devices for nuclear physics at RAON. It focuses on the nuclear symmetry energy at supra-saturation densities. The LAMPS Collaboration has developed and constructed various detector elements, including a time projection chamber(TPC) and a forward neutron detector array. From the positron beam test, the drift velocity of the secondary electrons in the TPC is 5:3±0:2 cm/ls with P10 gas mixture, and the position resolution for pads with dimensions of 4×15 mm^2 is in the range of$ 0.6–0.8 mm, depending on the beam position. From the neutron beam test, the energy resolution of the prototype neutron detector module is determined to be 3.4%, and theposition resolution is estimated to be better than 5.28 *** present, the construction of the LAMPS neutron detector system is in progress.

关键词：分光计精力用途朝鲜对称开发核物理学合作发展

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Prevalence of premature ejaculation in young and middle-aged men in Korea： a multicenter intemet-based survey from the Korean Andrological Society

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Asian Journal of Andrology 2010年第6期12卷 880-889页

作者： Hyun jun Park Jong Kwan Park Kwangsung Park Sung Won Lee Sae-Woong kim Dae Yul Yang DU Geon moon Kweon-Sik Min Ki-Hak moon Sang-Kuk Yang Jae Seog Hyun Nam Cheol Park Deparment of Urology Pusan National University School of medicine Busan 602739 Korea Deparment of Urology Chonbuk National University Medical School Jeonju 561712 Korea Deparment of Urology Chonnam National University Medical School Gwangju 501746 Korea Deparment of Urology Sungkyunkwan Univer＆ty School of Medicine Seou1135 710 Korea Deparment of Urology Catholic University College of Medicine Seoul 137701 Korea Deparment of Urology Hallym University College of Medicine Seoul 13 7701 Korea Deparment of Urology Korea University College of Medicine Seoul 152703 Korea Deparment of Urology lnje University College of Medicine Busan 614735 Korea Deparment of Urology Yeungnam University College of Medicine Daegu 705717 Korea Deparment of Urology Konkuk University School of Medicine Chungju 380704 Korea Deparment of Urology Gyeongsang National University School of Medicine Jinju 660702 Korea

In this study, we aimed to investigate the prevalence and perception of premature ejaculation （PE） in young and middle-aged Korean men. The study was conducted using an Internet-assisted questionnaire. A total of 2 037 Korean male adults, aged 20 years or older, were randomly sampled based on age and residency. The questionnaire developed by the PE Study Group of the Korean Andrological Society includes four categories （overall sexual function, symptoms, distress and treatment） with a total of 16 questions. For each question, symptoms were evaluated by a scale ranging from 0 to 10. Intravaginal ejaculation latency time was ‘5-10 min＇ in 38.6%, followed by ‘longer than 10 min＇ in 2919%, ‘2-5 min＇ in 23.6%, ‘1-2 min＇ in 5.4% and ‘shorter than 1 min＇ in 2.5%. In our series, 27.5% of respondents reported having PE. Control over ejaculation within a recent 3-month period was 6.2 points on average. Respondent complaints of PE-related stress averaged 7.1 points and stress-related complaints from sexual partners averaged 7.1 points. The effect of PE on sexual life was 6.8 points. Of the respondents determined as having PE, 42.6% responded that they were inclined to receive treatment. Results from this study suggest that the prevalence of PE diagnosed by the respondent on his own was approximately 27.5% in young and middle-aged men in Korea. PE-related stress had a significant effect on the stress, sexual activity and quality of life of the respondent and his sexual partner.

关键词： epidemiology premature ejaculation questionnaire sexual dysfunction

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Timing of upper gastrointestinal endoscopy does not influence short-term outcomes in patients with acute variceal bleeding

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World Journal of Gastroenterology 2018年第44期24卷 5025-5033页

作者： Jeong-Ju Yoo Young Chang Eun Ju Cho Ji Eun moon Sang Gyune kim Young Seok kim Yun Bin Lee Jeong-Hoon Lee Su Jong Yu Yoon jun kim jung-Hwan Yoon Department of Gastroenterology and Hepatology Soonchunhyang University school of Medicine Bucheon 14584 South Korea Department of Internal Medicine and Liver Research Institute Seoul National University College of Medicine Seoul 03080 South Korea Department of Biostatistics Clinical Trial Center Soonchunhyang University Bucheon Hospital Bucheon 14584 South Korea

AIM To examine the association between the timing of endo-scopy and the short-term outcomes of acute variceal bleeding in cirrhotic *** This retrospective study included 274 consecutive patients admitted with acute esophageal variceal bleeding of two tertiary hospitals in Korea. We adjusted confounding factors using the Cox proportional hazards model and the inverse probability weighting （IPW） method. The primary outcome was the mortality of patients within 6 *** A total of 173 patients received urgent endoscopy （ i.e. , ≤ 12 h after admission）, and 101 patients received non-urgent endoscopy （〉 12 h after admission）. The 6-wk mortality rate was 22.5% in the urgent endoscopy group and 29.7% in the non-urgent endoscopy group, and there was no significant difference between the two groups before （P = 0.266） and after IPW （P = 0.639）. The length of hospital stay was statistically different between the urgent group and non-urgent group （P = 0.033）; however, there was no significant difference in the in-hospital mortality rate between the two groups （8.1%vs 7.9%, P = 0.960）. In multivariate analyses, timing of endoscopy was not associated with 6-wk mortality （hazard ratio, 1.297; 95% confdence interval, 0.806-2.089; P = 0.284）.CONCLUSION In cirrhotic patients with acute variceal bleeding, the timing of endoscopy may be independent of short-term mortality.

关键词： Cirrhosis Endoscopy Upper gastrointestinal bleeding Gastroesophageal varices Timing

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Changing trends and characteristics of peptic ulcer disease:A multicenter study from 2010 to 2019 in Korea

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World Journal of Gastroenterology 2023年第44期29卷 5882-5893页

作者： Yoon Jin Choi Tae jun kim Chang Seok Bang Yong Kang Lee moon Won Lee Su Youn Nam Woon Geon Shin Seung In Seo Department of Internal Medicine National Cancer CenterGoyang-si 13620South Korea Department of Internal Medicine Samsung Medical CenterSeoul 06351South Korea Department of Internal Medicine Chuncheon Sacred Heart HospitalChuncheon 24253South Korea Department of Internal Medicine National Health Insurance Service Ilsan HospitalGoyang-si 10444South Korea Department of Internal Medicine Pusan National University School of MedicineBusan 50463South Korea Division of Gastroenterology and Hepatology Department of Internal MedicineKyungpook National University Chilgok HospitalDaegu 41404South Korea Department of Internal Medicine Kangdong Sacred Heart HospitalHallym University College of MedicineSeoul 05355South Korea Institute for Liver and Digestive Diseases Hallym UniversityChuncheon 24253South Korea Division of Gastroenterology Department of Internal MedicineKangdong Sacred Heart HospitalSeoul 05355South Korea

BACKGROUND The clinical trend and characteristics of peptic ulcer disease(PUD)have not fully been investigated in the past *** To evaluate the changing trends and characteristics of PUD according to age and METHODS We analyzed seven hospital databases converted into the Observational Medical Outcomes Partnership-Common Data Model between 2010 and *** classified patients with PUD who underwent rapid urease tests or Helicobacter pylori(***)serology into three groups:***-related,drug[nonsteroidal anti-inflammatory drugs(NSAIDs)or aspirin]-related,and idiopathic(***/NSAID/aspirin-negative)PUD and compared the yearly trends and characteristics among the three *** We included 26785 patients in 7 databases,and the proportion of old age(≥65 years)was 38.8%.The overall number of PUD exhibited no decrease,whereas PUD in old age revealed an increasing trend(P=0.01 for trend).Of the 19601 patients,41.8%had ***-related,36.1%had drug-related,and 22.1%had idiopathic *** PUD exhibited a decreasing trend after 2014(P=0.01),drug-related PUD demonstrated an increasing trend(P=0.04),and idiopathic PUD showed an increasing trend in the old-age group(P=0.01)during 10 *** with drug-related PUD had significantly more comorbidities and concomitant ulcerogenic *** idiopathic PUD group had a significantly higher number of patients with chronic liver *** With the aging population increase,the effects of concomitant ulcerogenic drugs and preventive strategies should be investigated in drug-induced *** studies are required to clarify the relationship between idiopathic PUD and chronic liver disease.

关键词： Peptic ulcer disease Drug Idiopathic Trend Characteristics

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电沉积Mo和Mo-Co合金纳米线用于互联电阻的电阻率改性

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Engineering 2024年第1期32卷 127-137页

作者： jun Hwan moon Taesoon kim Youngmin Lee Seunghyun kim Yanghee kim Jae-Pyoung Ahn jungwoo Choi Hyuck Mo Lee Young Keun kim Department of Materials Science and Engineering Korea UniversitySeoul 02841Republic of Korea Department of Materials Science and Engineering Korea Advanced Institute of Science and EngineeringDaejeon 34141Republic of Korea Advanced Analysis Center Korea Institute of Science and TechnologySeoul 02792Republic of Korea Research Resources Division Korea Institute of Science and TechnologySeoul 02792Republic of Korea

Achieving historically anticipated improvement in the performance of integrated circuits is challenging,due to the increasing cost and complexity of the required technologies with each new *** overcome this limitation,the exploration and development of novel interconnect materials and processes are highly desirable in the microelectronics ***(Mo)is attracting attention as an advanced interconnect material due to its small resistivity size effect and high cohesive energy;however,effective processing methods for such materials have not been widely ***,we investigate the electrochemical behavior of ions in the confined nanopores that affect the electrical properties and microstructures of nanoscale Mo and Mo-Co alloys prepared via template-assisted *** in an electrolyte allow the deposition of extremely pure metal materials,due to their interac-tion with metal ions and *** this study,boric acid and tetrabutylammonium bisulfate(TBA)were added to an acetate bath to inhibit the hydrogen evolution *** accelerated the reduction of Mo at the surface by inducing surface conduction on the *** Mo nanowires with a 130 nm diameter synthesized through high-aspect-ratio nanopore engineering exhibited a resistivity of(63.0±17.9)μΩ*** also evaluated the resistivities of Mo-Co alloy nanowires at various compo-sitions toward replacing irreducible conventional barrier/liner *** intermetallic compound formed at a Mo composition of 28.6 at%,the resistivity of the Mo-Co nanowire was(58.0±10.6)μΩcm,indicat-ing its superior electrical and adhesive properties in comparison with those of conventional barriers such as TaN and ***,density functional theory and non-equilibrium Green's function calcula-tions confirmed that the vertical resistance of the via structure constructed from Mo-based materials was 21%lower than that of a conventional Cu/Ta/TaN structure.

关键词： Molybdenum Molybdenum-cobalt Interconnect Microstructure Electrodeposition Density functional theory

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Tc-99m ciprofloxacin imaging in diagnosis of chronic bacterial prostatitis

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Asian Journal of Andrology 2003年第3期5卷 179-183页

作者： Ji-Kan Ryu Seong-Min Lee Do-Whan Seong jun-Kyu Suh Sungeun kim Wonsick Choe Yeonsook moon Soo-Hwan Pai Department of Urology Inha University School of MedicineJncheon 400103Korea Department of Nuclear Medicine Inha University School of MedicineIncheon 400103Korea Department of Clinical Patholgy Inha University School of MedicineJncheon 400103Korea

Aim: To investigate the value of Tc-99m ciprofloxacin imaging in the differential diagnosis of chronic bacterial prostatitis. Methods: The study included 4 normal subjects as the negative controls, 2 patients with acute prostatitis or cystourethritis as the positive controls and 59 patients diagnosed as chronic bacterial prostatitis or chronic pelvic pain syndrome by traditional laboratory tests. In every subject, the single photon emission computerized tomography images were obtained 3 h after intravenous injection of Tc-99m Ciprofloxacin. The results of the imaging were compared with those of laboratory tests. Results: On the images, negative uptake was observed in all normal subjects, while strong hot uptake, in the whole prostate of acute prostatitis patients and in the whole urethra of acute cystourethritis patients. In 13 (68 %) of 19 patients categorized as chronic bacterial prostatitis by standard laboratory tests, hot uptake with less intensity than that of acute prostatitis was observed in the prostate area around the prostatic urethra. Negative uptake in the prostate was observed in 6 of 19 patients (32 %) categorized as chronic bacterial prostatitis. Interestingly, hot uptake in the prostate was exhibited in 28 (70 %) of the 40 patients categorized as chronic pelvic pain syndrome. Conclusion: Tc-99m ciprofloxacin imaging is helpful in the differential diagnosis of prostatitis syndrome.

关键词： prostate prostatitis pelvic pain syndrome urinary tract infections radioisotope diagnostic techniques

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