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Edible Growth Medium: A New Window for Probiotic Research

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Advances in Microbiology 2020年第2期10卷 39-51页

作者： Md. Nur Hossain Annana Akter Sanjida Humayan Liton Chandra Mohanto Shamima Begum monzur morshed ahmed Institute of Food Science and Technology Bangladesh Council of Scientific and Industrial Research，Dhaka Bangladesh Department of Microbiology Jagannath University Dhaka Bangladesh

Conventional growth media for microbes contains lots of non-edible components that are harmful to consume when the organism is ready to intake—like probiotics growing in chemical media (Lactobacillus grown in MRS media). The study was conducted to develop an edible and low-cost growth media that supports the growth of probiotics Lactic Acid bacteria for the enhancement of probiotic research. The 04 isolates of Lactobacillus (L. plantarum, L. rhamnosus, L. ferciminis and L. bifarmentans) and 02 Bifidobacterium (B. infantis and B. Bifidum) were used for the evaluation of medium efficacy. To formulate the edible culture media, 04 vegetables and 02 pulses were used. The media was formulated in different formulations. For Lactobacillus, maximum growth was observed at MM-2 media formulation that was about 15.62 log CFU/ml for L. plantarum and compared to MRS media that was 11.78 log CFU/ml. Bifidobaterium showed the highest viability at MW-1 edible media formulation which was about 12.72 log CFU/ml whereas in Bifidobacteria selective media the cell viability was 12.48 log CFU/ml. The edible media has no toxic or unhealthy effect on mice while trailing in an animal model and shows excellent results in encapsulation with alginate. In comparison with the performance of traditional chemical media, the formulated media were found to be cost-effective and safe for human consumption.

关键词： Edible Media Lactobacillus Probiotic Low Cost Mass Culture

Identification of Aflatoxigenic Fungi and Detection of Their Aflatoxin in Red Chilli (<i>Capsicum annuum</i>) Samples Using Direct Cultural Method and HPLC

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Advances in Microbiology 2018年第1期8卷 42-53页

作者： Md. Nur Hossain Asma Talukder Farhana Afroze Md. Matiur Rahim Shamima Begum Md. Zahurul Haque monzur morshed ahmed Industrial Microbiology Laboratory Institute of Food Science and Technology (IFST) Bangladesh Council of Scientific and Industrial Research (BCSIR) Dhanmondi Dhaka Bangladesh Department of Genetics and Biotechnology Noakhali Science and Technology University Noakhali Bangladesh Department of Microbiology Jagganath University Dhaka Bangladesh Food Toxicology Research Laboratory Institute of Food Science and Technology (IFST) Bangladesh Council of Scientific and Industrial Research (BCSIR) Dhanmondi Dhaka Bangladesh

Aflatoxins are the potential lethal toxin produced by Aspergillus sp. important health hazard throughout the world. In this study, 26 Aspergillus sp. have been isolated from 50 samples of red chilli collected throughout the country. These 26 isolates were grown primarily on agar media to identify the aflatoxin producing species. It is possible to distinguish A. flavus strains from other Aspergillus sp. developing orange colour on the reverse of the plates. The Coconut Cream Agar (CCA) is used to detect aflatoxin producer strains having blue fluorescence when exposed to a UV-light. Several other media were used for morphological characteristics of Aspergillus sp. Out of 26 isolates, four isolates were confirmed as Aspergillus sp. These isolates were subjected to cross contamination with freshly ground, sterile maize and after 15 days of incubation the contaminated maize were analyzed by HPLC and found aflatoxin in each of the sample containing 186 ppb (max.). This study was conducted to assay the ability to produce aflatoxins by the Aspergillus spp. isolated from red chilli (Capsicum annuum L. Solanaceae) available throughout the country. The results found in the experiment are much more behind the acceptable limit according to some international standard. As red chilli is a widely used spice in Bangladesh, the proper controlling measures may be taken for controlling the surveillance of aflatoxinic fungi like as use of bio-pesticides, proper drying method and storage conditions.

关键词： Aflatoxin Aspergillus Detection HPLC Red Chilli

Preoperative Serum Albumin Levels and Postoperative Acute Kidney Injury in Off-Pump Coronary Artery Bypass Surgery: A Single-Center Study in Bangladesh

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World Journal of Cardiovascular Surgery 2024年第8期14卷 131-144页

作者： Ahmad Pear Salahuddin Md. Abir Tazim Chowdhury Munama Magdum Dewan Iftakher Reza Chowdhury Nittya Nanda Pal Md. Nahedul morshed Md. Zafar-Al-Nimari Latifa Nasrin Farooque ahmed Department of Cardiac Surgery National Heart Foundation Hospital and Research Institute (NHFH&RI) Dhaka Bangladesh Department of Cardiothoracic and Vascular Surgery Evercare Hospital Dhaka Dhaka Bangladesh National Institute of Preventive and Social Medicine Dhaka Bangladesh Mother and Child Welfare Center Cumilla Bangladesh

Background: Serum albumin, a vital plasma protein, helps maintain intravascular colloidal osmotic pressure, cardiac output, and renal function. Low preoperative serum albumin is linked to poor outcomes, including acute kidney injury (AKI), after off-pump coronary artery bypass (OPCAB) surgery. This study aimed to assess the relationship between preoperative serum albumin levels and early postoperative renal injury. Methods: This prospective comparative cross-sectional study was conducted from August 2019 to February 2021 at the National Heart Foundation Hospital & Research Institute, Bangladesh. It included 160 adult patients with normal preoperative renal function undergoing OPCAB. Patients were divided into two groups: Group A (serum albumin ≥ 4.0 gm/dl) and Group B (serum albumin Results: Preoperative serum albumin was significantly different between groups (Group A: 4.21 ± 0.05 gm/dl, Group B: 3.69 ± 0.04 gm/dl, p = 0.028). Group B had a higher incidence of hypertension (71.25% vs. 51.25%, p st and 3rd postoperative days were higher in Group B (p th day. Postoperative AKI occurred in 18.75% of Group A and 36.25% of Group B. Multivariate regression indicated that low preoperative serum albumin is an independent risk factor for postoperative AKI (p = 0.012, OR = 1.815, CI: 0.675 - 1.162). Conclusion: Preoperative serum albumin level is a valuable predictor of postoperative renal function. Ensuring high normal serum albumin levels before surgery can help minimize the risk of postoperative AKI.

关键词： Serum Albumin Acute Kidney Injury (AKI) Off-Pump Coronary Artery Bypass Surgery Bangladesh

Effect of Trimetazidine on Functional Capacity in Patient with Ischaemic Cardiomyopathy (TOFCAPI)

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International Journal of Clinical Medicine 2024年第2期15卷 55-67页

作者： Mohsin ahmed Golam morshed Mahmood Hasan Khan Md. Arifur Rahman Muhammed Aminur Razzaque Shariful Islam Sayeedur Rahman Khan Chowdhury Md. Kudrat-E-Khuda Salahuddin Anis Shiblee Md. Habibur Rahman Department of Cardiology National Institute of Cardiovascular Diseases Dhaka Bangladesh Department of Cardiology Government Employee’s Hospital Dhaka Bangladesh Department of Cardiology Delta Hospital Ltd. Dhaka Bangladesh IPDI Foundation Dhaka Bangladesh

Objective: This study aimed to evaluate the efficacy of trimetazidine on exercise capacity via a six-minute walk test in patients with ischaemic cardiomyopathy and also evaluate the effect of trimetazidine on left ventricular function via echocardiography in the same population. Methods: This prospective observational study, conducted at the National Institute of Cardiovascular Diseases in Dhaka, Bangladesh, enrolled 200 patients with ischaemic cardiomyopathy and a depressed left ventricular ejection fraction (LVEF Results: In this study (n = 200) of ischaemic cardiomyopathy patients, the mean age was 58 years, with 76% of the patients being male. All study subjects received GDMT (Guideline-Directed Medical Therapy) for angina and heart failure. Those who received the modified released form of trimetazidine developed lesions during the 1st and 2nd follow-ups, during which the LVEF, LVIDd, and six-minute walk distance significantly improved (p Conclusion: The findings of the present study demonstrated that the addition of modified-release trimetazidine to GDMT can improve exercise capacity and left ventricular function in patients with ischaemic cardiomyopathy.

关键词： Bangladesh Heart Failure Exercise Capacity Trimetazidine Ischaemic Cardiomyopathy

Comparative assessment of total polyphenols, antioxidant and antimicrobial activity of different tea varieties of Bangladesh

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Asian Pacific Journal of Tropical Biomedicine 2017年第4期7卷 352-357页

作者： Yead morshed Nibir ahmed Faisal Sumit Anwarul Azim Akhand Nazmul Ahsan Mohammad Shahnoor Hossain Department of Genetic Engineering and Biotechnology University of DhakaDhaka 1000Bangladesh

Objective: To determine the total polyphenol content, antioxidant activity and antibacterial properties of the extracts of different Bangladeshi tea varieties such as flowery broken orange pekoe, broken orange pekoe, red dust and green ***: Total phenolic and flavonoid contents were determined by Folin–Ciocalteu and aluminum chloride colorimetric assay, *** antioxidant capacity was determined by ferric ion reducing antioxidant power and phosphomolybdenum *** activity was evaluated by disc diffusion method in agar plate and subsequently, the minimum inhibitory concentration was determined by broth dilution ***: Total phenolic and flavonoid contents were significantly higher(P < 0.05) in green tea compared to other three black tea *** green tea also showed a higher free radical scavenging and antioxidant activities than all the other tea varieties tested(P < 0.05).In addition, the extracts of all four tea varieties showed inhibitory activity against several pathogenic bacteria and also the same trend of higher antimicrobial activity of green tea than other tea varieties was ***: Taken together, the results of this study demonstrated that Bangladeshi tea,especially the green tea, may act as a substitute for natural antioxidants and as a promising antibacterial agent for beneficial influence in human health.

关键词： Tea extracts Antioxidant activity Antibacterial activity Total phenolic content

Trend Analysis of Large-Scale Twitter Data Based on Witnesses during a Hazardous Event: A Case Study on California Wildfire Evacuation

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World Journal of Engineering and Technology 2021年第2期9卷 229-239页

作者： Syed A. morshed Khandakar Mamun ahmed Kamar Amine Kazi Ashraf Moinuddin Department of Civil and Environmental Engineering College of Engineering and Computing Florida International University Miami Florida United States School of Computing and Information Sciences Florida International University Miami Florida United States Department of Computer Engineering University of Memphis Memphis Tennessee United States

Social media data created a paradigm shift in assessing situational awareness during a natural disaster or emergencies such as wildfire, hurricane, tropical storm etc. Twitter as an emerging data source is an effective and innovative digital platform to observe trend from social media users’ perspective who are direct or indirect witnesses of the calamitous event. This paper aims to collect and analyze twitter data related to the recent wildfire in California to perform a trend analysis by classifying firsthand and credible information from Twitter users. This work investigates tweets on the recent wildfire in California and classifies them based on witnesses into two types: 1) direct witnesses and 2) indirect witnesses. The collected and analyzed information can be useful for law enforcement agencies and humanitarian organizations for communication and verification of the situational awareness during wildfire hazards. Trend analysis is an aggregated approach that includes sentimental analysis and topic modeling performed through domain-expert manual annotation and machine learning. Trend analysis ultimately builds a fine-grained analysis to assess evacuation routes and provide valuable information to the firsthand emergency responders.

关键词： Wildfire Evacuation Twitter Large-Scale Data Topic Model Sentimental Analysis Trend Analysis

Y-Shaped Intra-Scleral Fixated Lens versus Retro-Pupillary Iris Claw Lens in the Treatment of Aphakia

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Open Journal of Ophthalmology 2019年第2期9卷 105-124页

作者： ahmed Mohamed morshed Mahmoud Nasr Aldeeb Adel Kamal Abdeen Salah Mohamed Almosalamy Ophthalmology Department Faculty of Medicine Zagazig University Zagazig Egypt

Background: Many reasons can lead to an aphakia without adequate capsular support for implantation of a posterior chamber intraocular lens (IOL), such as intraoperative unintentional rupture of posterior capsule during phacoemulsification, planned intracapsular cataract extraction, ocular trauma and lens dislocation due to congenital and acquired causes. Purpose: To compare Y-shaped intra-scleral fixation of a posterior chamber IOL with retro pupillary fixation of an iris-claw intraocular lens (IOL) for Aphakic eyes without sufficient capsular support as respects safety, visual recovery and complications of both methods. Patients and Methods: One hundred Aphakic eyes were arbitrarily distributed between two groups. Group A included 50 eyes treated with retropupillary fixation of iris claw lens and group B included 50 eyes treated with Y-shaped intra-scleral fixation technique. Preoperative, intraoperative and postoperative data were analysed including best corrected visual acuity (BCVA), intraocular pressure (IOP), surgical time, intraoperative problems, IOL malposition and postoperative complications. Following up on patients was carried out for at least six months. Results: The mean duration of surgery was 21 ± 5.3 min in group A and was 53.4 ± 6.9 min in group B (p-value 0.05). IOL tilt was found in 0 (0%) eyes in group A and in 5 (10%) eyes in group B (p 0.05). Conclusion: The results of our study indicated that both methods are satisfactory in correcting aphakia without sufficient capsular support as regards postoperative best corrected visual acuity (BCVA);however the surgical technique of retropupillary iris claw lens is easier, shorter, with low intra- and postoperative complications and safer than those used for intra-scleral fixation of IOL. But for eyes which lack both iris and capsular support, a scleral fixation of a posterior chamber IOL remains the only option.

关键词： Aphakia Iris-Claw Intraocular Lens (IOL) Y-Shaped Intra Scleral Fixation