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Survival of rat sciatic nerve segments preserved in storage solutions ex vivo assessed by novel electrophysiological and morphological criteria

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Neural Regeneration Research 2023年第9期18卷 2082-2088页

作者： Liwen Zhou monzer alatrach Ted Zhao Paul Oliphint George D.Bittner Department of Neuroscience University of Texas at AustinAustinTXUSA

Most organ or tissue allografts with viable cells are sto red in solutions ex vivo for hours to seve ral days.Most allografts then require rapid host revascula rization upon transplantation to maintain donor-cell functions(e.g.,cardiac muscle contra ctions,hepatic secretions).In contrast,peripheral nerve allografts stored ex vivo do not require revascularization to act as scaffolds to guide outgrowth by host axons at 1-2 mm/d,likely aided by viable donor Schwann cells.Using current storage solutions and protocols,axons in all these donor orga n/tissue/nerve transplants are expected to rapidly become non-viable due to Wallerian degeneration within days.Therefore,ex vivo storage solutions have not been assessed for preserving normal axonal functions,i.e.,conducting action potentials or maintaining myelin sheaths.We hypothesized that most or all organ storage solutions would maintain axonal viability.We examined several common organ/tissue storage solutions(University of Wisconsin Cold Storage Solution,Normosol-R,Normal Saline,and La ctated Ringe rs) for axonal viability in rat sciatic nerves ex vivo as assessed by maintaining:(1) conduction of artificially-induced compound action potentials;and(2) axonal and myelin morphology in a novel assay method.The ten diffe rent storage solution conditions for peripheral nerves with viable axons(PNVAs) diffe red in their solution composition,osmolarity(250-318 mOsm),temperature(4℃ vs.25℃),and presence of calcium.Compound action potentials and axonal morphology in PNVAs were best maintained for up to 9 days ex vivo in calcium-free hypotonic diluted(250 mOsm) Normosol-R(dNR) at 4℃.Surprisingly,compound action potentials were maintained for only 1-2 days in UW and NS at 4℃,a much shorter duration than PNVAs maintained in 4℃ dNR(9 days) or even in 25℃ dNR(5 days).Viable axons in peripheral nerve allografts are critical for successful polyethylene glycol(PEG)-fusion of viable proximal and distal ends of host axons with viable donor axons to repair segmental-loss peripheral nerve i

关键词： axonal morphometrics axonal viability calcium osmolarity peripheral nerve grafts peripheral nerve injury temperature tissue storage solutions tonicity Wallerian degeneration

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Spatial and Temporal Analysis of Maximum and Minimum Temperature Trends in Northern Sudan during (1990-2019)

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Journal of Geoscience and Environment Protection 2024年第5期12卷 266-288页

作者： Elhag Gamreldin monzer Hamadalnel Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters School of Atmospheric Sciences Nanjing University of Information Science and Technology Nanjing China Sudan Meteorological Authority (SMA) Khartoum Sudan Department of Astronomy and Meteorology Faculty of Science and Technology Omdurman Islamic University Omdurman Sudan

The study addresses an urgent and globally significant issue of climate change by focusing on the detailed spatial and temporal analysis of temperature trends in Northern Sudan. It fills a critical research gap by providing localized data over a substantial period (1990-2019), which could help in understanding the nuanced impacts of climate change in Sahel regions like Northern Sudan. In addition, the comprehensive coverage of both spatial and temporal dimensions, supported by a substantial dataset from five meteorological stations, provides a thorough understanding of the subject area. The utilization of robust statistical methods (Mann-Kendall test and Sen’s slope analysis) for analyzing temperature trends adds scientific rigor and credibility to the findings. Our results reveal a consistently increasing trend in maximum temperatures across most stations, particularly during the hot season (AMJ). However, the wet season (JAS) shows high maximum temperatures but no significant trend. Moreover, significant increasing trends in minimum temperatures were observed in all stations except Abu Hamed, where the trend, although increasing, did not reach statistical significance during the hot and cold seasons, and the coldest temperatures were observed during the cold season. These findings underscore the complex temperature dynamics in Northern Sudan and highlight the need for continued monitoring and adaptive measures in response to ongoing climate changes in the region.

关键词： Climate Change Northern Sudan Temperature Trend Seasonal Analysis

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Novel therapeutic diiminoquinone exhibits anticancer effects on human colorectal cancer cells in two-dimensional and threedimensional in vitro models

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World Journal of Gastroenterology 2022年第33期28卷 4787-4811页

作者： Alissar monzer Kevork Wakimian Farah Ballout Samar Al Bitar Amani Yehya Mariam Kanso Nour Saheb Ayman Tawil Samer Doughan Maher Hussein Deborah Mukherji Walid Faraj Hala Gali-Muhtasib Wassim Abou-Kheir Department of Biology American University of BeirutBeirut 1107-2020Lebanon Department of Anatomy Cell Biology and Physiological SciencesAmerican University of BeirutBeirut 1107-2020Lebanon Department of Surgery American University of Beirut Medical CenterBeirut 1107-2020Lebanon Department of Pathology and Laboratory Medicine American University of Beirut Medical CenterBeirut 1107-2020Lebanon Department of Internal Medicine American University of Beirut Medical CenterBeirut 1107-2020Lebanon Department of Biology and Center for Drug Discovery American University of BeirutBeirut 1107-2020Lebanon

BACKGROUND Colorectal cancer(CRC) is the second leading cause of cancer-related mortality.Cancer stem cells(CSCs) in CRC, which are spared by many chemotherapeutics,have tumorigenic capacity and are believed to be the reason behind cancer relapse. So far, there have been no effective drugs to target colon CSCs. Diiminoquinone(DIQ) has shown promising effects on targeting colon cancer.However, there is limited research on the effects of DIQ on eradicating CSCs in CRC.AIM To investigate the anticancer potential of DIQ on colon CSCs in two-dimensional(2D) and three-dimensional(3D) models using colonospheres and patient-derived organoids.METHODS Various 2D methods have been used to assess the effect and the mechanism of DIQ on HCT116and HT29 cell lines including cell proliferation and viability assays, migration and invasion assays,immunofluorescence staining, and flow cytometry. The potency of DIQ was also assessed in 3D culture using the sphere formation assay and colon cancer patient-derived organoid model.RESULTS Our results showed that DIQ significantly inhibited cell proliferation, migration, and invasion in HCT116 and HT29 cell lines. DIQ treatment induced apoptosis along with an accumulation of HCT116 and HT29 cancer cells in the sub-G1 region and an increase in reactive oxygen species in both CRC cell lines. DIQ reduced sphere-forming and self-renewal ability of colon cancer HCT116and HT29 stem/progenitor cells at sub-toxic doses of 1 μmol/L. Mechanistically, DIQ targets CSCs by downregulating the main components of stem cell-related-catenin, AKT, and ERK oncogenic signaling pathways. Potently, DIQ displayed a highly significant decrease in both the count and the size of the organoids derived from colon cancer patients as compared to control and 5-fluorouracil conditions.CONCLUSION This study is the first documentation of the molecular mechanism of the novel anticancer therapeutic DIQ via targeting CSC, a promising compound that needs further investigation.

关键词： Diiminoquinone Anticancer activity Colorectal cancer Cancer stem cells Patient-derived organoids Colonospheres

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油气藏保存温度的碳同位素分析

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石油地质科技动态 2001年第1期 42-51,28页

作者： monzer Makhous 张炎昕

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撒哈拉地台的地质和构造特征以及沉积盆地演化轮廓

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石油地质科技动态 2001年第1期 1-8页

作者： monzer Makhous 白振端

在撒哈拉地台上，从南部的霍加尔山开始，可以观察到许多南北走的隆起（阿姨吉德尔比亚德－哈西边斯欧德，提克赫姆伯卡－扎尔扎伊廷－阿勒拉尔，伊贾伦－姆扎卡等），这些隆起之间是宽阔的复向斜，下伏许多与其同走向的坳陷。因此，东... 详细信息

在撒哈拉地台上，从南部的霍加尔山开始，可以观察到许多南北走的隆起（阿姨吉德尔比亚德－哈西边斯欧德，提克赫姆伯卡－扎尔扎伊廷－阿勒拉尔，伊贾伦－姆扎卡等），这些隆起之间是宽阔的复向斜，下伏许多与其同走向的坳陷。因此，东撒哈拉复向斜可以分为两个坳陷，南部是伊利济坳陷，北部是东大沙漠坳陷（古达迷斯坳陷）。中撒哈拉复向斜也下伏两个坳陷，其中，穆维迪尔坳陷位于南部，瓦德米亚坳陷们于北部。在西撒哈拉复向斜内发现了阿赫奈特、拉甘、廷杜夫、提米蒙和贝沙尔等缺陷（图1和2）。Guiraud等（1987）把这些盆地分为北撒啦盆地和南撒啦盆地，而Legrand（1985）和Whiteman（1972）则对这些盆地的地层作了详细描述。

关键词：油气藏撒哈拉地台地质构造特征沉积盆地演化

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非构造圈闭及其成因

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吐哈油气 2001年第3期6卷 91-99页

作者： monzer Makhous 李彦兰

地质研究经验表明:北非撒哈拉地台中的坳陷。如瓦得米亚、古达米斯和伊利济等坳陷,为研究地层不整合地带内的油气藏和由于岩相变化产生的岩性模式提供了最好的场所。如上所述,在三叠地区,沉积层中发育有两个主要的构造层,即被区域性海... 详细信息

地质研究经验表明:北非撒哈拉地台中的坳陷。如瓦得米亚、古达米斯和伊利济等坳陷,为研究地层不整合地带内的油气藏和由于岩相变化产生的岩性模式提供了最好的场所。如上所述,在三叠地区,沉积层中发育有两个主要的构造层,即被区域性海西期不整合面分隔的古生界和中生界构造层(图1)。在哈西迈斯欧德穹隆顶部,寒武纪沉积层出露在海西期不整合面上,而在提勒盖姆特隆起上甚至可发现更古老的下寒武统地层(图1)。在这些大型隆起斜坡的海西期不整合面之上被地质年代较新且连续沉积的古生代岩层所覆盖。

关键词：非构造圈闭三叠系岩性圈闭不整合面地层圈闭储层沉积层坳陷三叠纪海西期

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非构造圈闭及其成因

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石油地质科技动态 2001年第1期 29-41页

作者：李彦兰 monzer Makhous

地质研究经验表明：非北撒哈拉地台中的坳陷，如瓦德米亚、古达米斯和伊利济等坳陷，为研究地层不整合带内的油气藏和由于岩相变化产生的岩性模式提供了最好的场所。如上所述，在三叠地区（Triassic Province），沉积层中有两个主要的... 详细信息

地质研究经验表明：非北撒哈拉地台中的坳陷，如瓦德米亚、古达米斯和伊利济等坳陷，为研究地层不整合带内的油气藏和由于岩相变化产生的岩性模式提供了最好的场所。如上所述，在三叠地区（Triassic Province），沉积层中有两个主要的构造层，即被区域性海西斯不整合面分隔的古生界和中生界构造层（图1）。在哈西边斯欧德穹隆顶部，寒武纪沉积层出露在海西斯不整合面上，而在提勒盖姆特隆起上甚至发现更古老的下寒武统地层（图1），在这些大型隆起斜坡的海西期不整合而之上牟代较新的古生代岩层所覆盖。这套古生代储层的顶部被不渗透的中生代岩层不整合遮挡，从而形成了与不整面有关的地层圈闭。

关键词：撒哈拉地台地层圈闭成因非构造圈闭油气藏

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三叠地区含油气地层的岩相和沉积环境

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石油地质科技动态 2001年第1期 9-28页

作者： monzer Makhous 白振瑞

对伊利济和古达米斯盆地复杂地层的研究使我们能够详细恢复古生代沉积的古地理条件。古地理恢复和相应的结论是根据以下研究结果得出的。

关键词：三叠地区含油气地层岩相沉积寒武系奥陶系

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