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Quantitative Detection of <i>Helicobacter pylori</i>by Real Time PCR in Drinking Water—Environmental and Public Health Risk Significance

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Open Journal of Medical Microbiology 2015年第3期5卷 118-127页

作者： Virginia Montero-Campos Shirley Arias-cordero Benedicto Valdés-Rodríguez monserrat jarquín-cordero Research & Chemical and Microbiology Services Center—CEQIATEC School of Chemistry Instituto Tecnológico de Costa Rica Cartago Costa Rica Biotechnology Research Center School of Biology Instituto Tecnológico de Costa Rica Cartago Costa Rica Water Laboratory Services Physicochemical University of Chiriqui Chiriqui City Panama

Helicobacter pylori (H. pylori) is bacteria considered to be present in half of the population and it is a public health problem worldwide. Most patients infected with H. pylori show no clinical symptoms;nonetheless, approximately 10% to 20% of these patients will develop peptic ulcers and 1% will develop gastric cancer. The International Agency for Research on Cancer has classified H. pylori as a Group 1 carcinogen, recognized as the only bacteria capable of producing cancer. Samples of drinking water (n = 44) from aqueducts with chlorination treatment in selected areas with high prevalence of gastric cancer were analyzed in Costa Rica. Samples of drinking water from Panamá (n = 44) from aqueducts supplying untreated water for human consumption in the province of Chiriquí were also analyzed. The molecular marker of H. pylori, glmM, was used, and to optimize the Real Time PCR (qPCR) technique, annealing temperature, concentration of primers and probe were standardized;also, by analyzing different standard curves, the best reaction conditions that allowed detecting and quantifying the gene were determined. The LightCycler® 480 II (LC480II) equipment from Roche Diagnostics GmbH was used, as well as the Absolute Quantification Analysis by means of the Second Derivative Maximum Method. In the case of the samples from Costa Rica, it was determined that 79.5% were positive for H. pylori;removing outlier high average, quantification of bacteria was determined in 3.6 × 103 copies/100 mL. For Panamá it was determined that 86% of the samples were found positive for the presence of H. pylori;removing outlier high average quantification of bacteria was determined at 3.3 × 102 copies/100 mL. The difference in values between the aqueducts in both countries revealed an environmental distribution of the bacteria of epidemiological interest in each case.

关键词： Helicobacter pylori Drinking Water Real Time PCR (qPCR) Chlorination Treatment

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Improving colorectal cancer screening programs

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World Journal of Gastroenterology 2024年第22期30卷 2849-2851页

作者： Oscar J cordero Lucia Mosquera-Ferreiro Iria Gomez-Tourino Department of Biochemistry and Molecular Biology University of Santiago de CompostelaSantiago de Compostela 15782Spain Centre for Research in Molecular Medicine and Chronic Diseases(CiMUS) University of Santiago de CompostelaSantiago de Compostela 15782GaliciaSpain

In this editorial we comment on the article by Agatsuma et al published in the World Journal of *** suggest policies for more effective colorectal *** is the main policy that has led to lower mortality rates in later years among the population that was eligible for *** is the gold standard tool for screening and has preventive effects by removing precancerous or early malignant ***,colonoscopy is an invasive process,and fecal tests such as the current hemoglobin immunodetection were developed,followed by endoscopy,as the general tool for population screening,avoiding logistical and economic *** so,participation and adherence rates are *** screening options are being developed with the idea that if people could choose between the ones that best suit them,participation in population-based screening programs would *** tests,such as a recent one that detects cell-free DnA shed by tumors called circulating tumor DnA,showed a similar accuracy rate to stool tests for cancer,but were less sensitive for advanced precancerous *** the time when the crosstalk between the immune system and cancer was being established as a new hallmark of cancer,novel immune system-related biomarkers and information on patients’immune parameters,such as cell counts of different immune populations,were studied for the early detection of colorectal cancer,since they could be effective in asymptomatic people,appearing earlier in the adenoma-carcinoma development compared to the presence of fecal ***26,for example,detected 80.37%of advanced *** reach as many eligible people as possible,starting at an earlier age than current programs,the direction could be to apply tests based on blood,urine or salivary fluid to samples taken during routine visits to the primary health system.

关键词： Mortality rates Colorectal cancer Screening Biomarker Fecal hemoglobin immunodetection Soluble sCD26 Colonoscopy Immunoscores

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Behavioural observation on Platycnemis latipes revealing novel function of males'patrol

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Zoological Systematics 2023年第2期48卷 140-146页

作者： Xin Yu Adolfo cordero-Rivera College of Life Sciences Chongqing Normal UniversityChongqing 401331P.R.China Universidade de Vigo ECOEVO LabEscola de Enxeñaría ForestalCampus UniversitarioPontevedra 36005Spain

Males of Platycnemis latipes (Platycnemididae, Odonata) have enlarged tibiae (fans) and patrol using a special longitudinal zig-zag flight. By means of focal observations and experimental manipulations of the colour of abdomen and fans of mature males, we suggest that the zig-zag flight is used to avoid unnecessary harassment from other males and has not the function to attract females. The fans of P. latipes are mainly used as a tool to threaten rivals, but their effectiveness seems limited. Both abdomen and fans of a mature male can reflect UV light strongly.

关键词： Damselfly enlarged tibiae zig-zag flight avoiding harassment UV reflecting

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Direct and cross-generational effects of reproduction on fitness and behavioral variability in male-biased environments

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Current Zoology 2023年第3期69卷 339-353页

作者： Iago Sanmartin-Villar Xin Yu Adolfo cordero-Rivera Universidade de Vigo ECOEVO LabEscola de Enxenaria ForestalCampus Universitario A Xunqueira36005 PontevedraGalizaSpain College of Life Sciences Chongqing Normal UniversityDaxuecheng Middle RdShapingba DistrictChongqing 401333China

Population structure determines individuals’interactions and trade-offs with evolutionary ***-biased populations increase intrasexual competition and intersexual harassment,reducing female resource acquisition,and thus,resources availability for the following *** analyzed direct and cross-generational effects of male harassment in two generations of damselflies(Odonata).We exposed adult females to treatments with different sex-ratio and density(balanced and male-biased)to modify the male harassment *** analyzed female fecundity,fertility,and number of faecal deposits as an indirect measure of resources *** studied female flight performance after repeated exposures to *** analyzed survivorship,development,exploration,thigmotaxis,and feeding latency of larvae produced by the experimental *** both generations,we analyzed four metrics of behavior:mean value,interindividual differences in plasticity,intra-individual unpredictability,and *** duration increased in male-biased treatment,whereas female resources acquisition and fertility *** that mated longer showed higher fecundity when they were exposed to balanced treatment,but not if they were exposed to male-biased *** from the male-biased treatment showed interindividual differences in plasticity and no repeatability in flight *** showed balanced sex-ratio and similar survivorship,development,and feeding latency independently of the parental treatment;however,females exposed to male-biased treatment produced offspring with higher differences in exploration plasticity and daughters less explorative and with higher unpredictable *** propose prolonged copulation as courtship at balanced sex-ratio but a cost to females under male-biased ***-generational effects in behavioral variability may be a mechanism to cope with predicted future environments.

关键词： activity environmental perception Ischnura graellsii male harassment sex allocation

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Strategic adjustment of copulatory plug size in a nematode

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Current Zoology 2019年第5期65卷 571-577页

作者： Jorge Canales-LAZCAnO Jorge Contreras-GARDUnO Carlos cordero Posgrado en Ciencias Biomedicas Institute)de EcologiaUNAMCircuito Exterior S/Nanexo al Jardfn Botanico exteriorCiudad UniversitariaCiudad de MexicoMexico Escuela nacional de Estudios Superiores Universidad Nacional Autonoma de MexicoUnidad MoreliaAntigua Carretera a Patzcuaro No.8701Col.Ex-Hacienda San Jose de la HuertaMoreliaMichoacanMexico Departamento de Ecologia Evolutiva Instituto de EcologlaUNAMCircuito Exterior S/Nanexo al Jardin Botanico exteriorCiudad UniversitariaCiudad de MexicoMexico

Copulatory plugs(CP)are substances produced during copulation that block the genital openings of the *** several species of nematoda,males produce CP that are thought to impede female remating and thus sperm *** relatively large size of the CP in several nematodes,and its evolutionary loss in self-fertilizing populations of Caenorhabditis elegans,suggests that CP are costly to *** CP production is costly,the application of basic concepts of strategic ejaculation theory suggests a modulated allocation of CP in response to sperm competition *** hypothesis led us to predict that males perceiving a higher risk of sperm competition will produce larger *** tested these ideas with the entomopathogenic,gonochoristic nematode Rhabditis *** first experiment provides evidence suggesting that production of CP is costly,because the size of CP is negatively affected by stressful conditions(high population density,small male adult size,and suboptimal food type).The results of our second experiment support the prediction that males adjust the size of CP to sperm competition risk:the average size of CP increased as the number of males competing for one female ***,our study supports the idea that in *** the production of CP is costly for males and that the size of the CP produced is influenced by sperm competition risk.

关键词： copulatory plug sexual selection sperm competition strategic ejaculation nematodes

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Different patterns of intestinal response to injury after arterial,venous or arteriovenous occlusion in rats

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World Journal of Gastroenterology 2009年第31期15卷 3901-3907页

作者： Francisco Javier Guzmán-de la Garza Carlos Rodrigo Cámara-Lemarroy Gabriela Alarcón-Galván Paula cordero-Pérez Linda Elsa Muoz-Espinosa nancy Esthela Fernández-Garza Department of Physiology Autonomous University of Nuevo LeónSchool of Medicine Pathologic Anatomy and Cytopathology Service "José Eleuterio González" University Hospital Liver Unit Department of Internal MedicineAutonomous University of Nuevo LeónSchool of Medicine

AIM： To investigate the differences in injury patterns caused by arterial, venous or arteriovenous mesenteric occlusion. METHODS： Male Wistar rats were separated equally into four groups. Occlusion was performed by clamping the superior mesenteric artery （A）, the mesenteric vein （V） or both （AV） for 30 min, followed by 60 min of reperfusion. A control group received sham surgery only. Intestinal sections were examined for histological damage and serum tumor necrosis factor-α （TnF-α）, endothelin-1 （ET-1）, P-selectin, antithrombin Ⅲ （AT Ⅲ） and soluble intracellular adhesion molecule-1 （ICAM-1） concentrations were measured. RESULTS： All groups showed significant mucosal injury compared to controls. Furthermore, mucosal injury was significantly more severe in the V and AV groups compared to the A group （3.6 ± 0.55, 3.4 ± 0.55 and 2± 0.71, respectively, P = 0.01）. ICAM-1 was similarly elevated in all groups, with no significant differences between the groups. P-selectin levels were significantly elevated in the V and AV groups but not the A group （1.4 ± 0.5 ng/mL, 2.52 ± 0.9 ng/mL and 0.02± 0.01 ng/mL, respectively, P = 0.01） and ET-1 was significantly elevated in the A and V groups but not the AV group （0.32 ± 0.04 pg/mL, 0.36 ± 0.05 pg/mL and 0.29 ± 0.03 pg/mL, respectively, P = 0.01） compared to sham controls. ATⅢ levels were markedly depleted in the V and AV groups, but not in the A group （29.1 ± 5.2 pg/mL, 31.4 ±21.8 pg/mL and 55.8± 35.6 pg/mL, respectively, P = 0.01）, compared to controls. Serum TnF-α was significantly increased in all groups compared to sham controls （1.32 ± 0.87 ng/mL, 1.79 ± 0.20 ng/mL and 4.4 ± 0.69 ng/mL, for groups A, V and AV, respectively, P = 0.01）, with higher values in the AV group. COnCLUSIOn： Different patterns of response to ischemia/reperfusion are associated with venous, arterial or arteriovenous occlusion. Venous and arteriovenous occlusion was associated with the most severe alterations

关键词： Arterial occlusion Rat Intestine Ischemia/ reperfusion Venous occlusion

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Analysis of Oseltamivir Resistance Substitutions in Influenza Virus Glycoprotein neuraminidase using a Lentivirus-Based Surrogate Assay System

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Virologica Sinica 2013年第2期28卷 81-91页

作者： Jennifer Tisoncik-Go Katie S cordero Lijun Rong Department of Microbiology and Immunology College of Medicine University of Illinois at Chicago Department of Microbiology University of Washington

Influenza A virus poses a great threat to global health, and oseltamivir (trade marked as Tamiflu), which targets influenza surface glycoprotein neuraminidase (nA), is used clinically as a major anti-influenza treatment. However, certain substitutions in nA can render an influenza virus resistant to this drug. In this study, using a lentiviral pseudotyping system, which alleviates the safety concerns of studying highly pathogenic influenza viruses such as avian influenza H5n1, that utilizes influenza surface glycoproteins (hemagglutinin or HA, and nA) and an HIV-core combined with a luciferase reporter gene as a surrogate assay, we first assessed the functionality of nA by measuring pseudovirion release in the absence or presence of oseltamivir. We demonstrated that oseltamivir displays a dose-dependent inhibition on nA activity. In contrast, a mutant nA (H274Y) is more resistant to oseltamivir treatment. In addition, the effects of several previously reported substitution nA mutants were examined as well. Our results demonstrate that this lentivirus-based pseudotyping system provides a quick, safe, and effective way to assess resistance to neuraminidase inhibitors. And we believe that as new mutations appear in influenza isolates, their impact on the effectiveness of current and future anti-nA can be quickly and reliably evaluated by this assay.

关键词： Influenza virus neuraminidase (nA) Oseltamivir Drug resistance

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The movement of ＂false antennae＂ in butterflies with ＂false head＂ wing patterns

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Current Zoology 2015年第4期61卷 758-764页

作者： Tania G. LOPEZ-PALAFOX Armando LUIS-MARTInEZ Carlos cordero Posgrado en Ciencias Biologicas Instituto de Ecologia Universidad Nacional Autonoma de Mexico Distrito Federal 04510 Mexico Museo de Zoologia Departamento de Biologia Evolutiva Facultad de Ciencias Universidad Nacional Autonoma de Mexico Distrito Federal 04510 Mexico Departamento de Ecologia Evolutiva Instituto de Ecologia Universidad Nacional Autonoma de M6xico Distrito Federal 04510 Mexico

In many butterfly species of the family Lycaenidae, the morphology and color pattern of the hind wings, together with certain behaviors, suggests the presence of a false head （FH） at the posterior end of the perching individual. This FH is consi- dered an adaptation to escape from visually oriented predators. A frequent component of the FH are the tails that presumably resemble the antennae, and the typical hind wings back-and-forth movement along the sagittal plane （HWM） performed while perching apparently move the tails in a way that mimics antennal movement. By exposing 33 individuals from 18 species of Lycaenidae to a stuffed insectivorous bird, we tested two alternative hypotheses regarding HWM. The first hypothesis proposes that, when the butterfly is observed at close range, the HWM distorts the shape of the false head thus reducing its deceiving effect and, therefore, selection will favor butterflies that stop moving their wings when a predator is close by; the second hypothesis says that an increase in the frequency of HWM improves its deflective effect when the butterfly confronts a predator at close range. Our results tend to support the second hypothesis because half of the butterflies started to move their hind wings or increased the rate of HWM when exposed to the stuffed bird; however a substantial proportion of butterflies （30%） stopped moving their hind wings or decreased the rate of HWM as expected from the first hypothesis. Our observations also showed that there is great variation in the rates of HWM, and demonstrated the existence of alternative ways of producing ＂vivid＂ movement of the hind wing tails （the ＂false antennae＂） in the absence of HWM [Current Zoology 61 （4）： 758-764, 2015].

关键词： Butterflies Lycaenidae Predator deception Anti-predator behavior Wing morphology

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nMS Flows on Three-Dimensional Manifolds with One Saddle Periodic Orbit

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Acta Mathematica Sinica,English Series 2004年第1期20卷 47-56页

作者： B.CAMPOS A.cordero J.Martinez ALFARO P.VInDEL Department Matemàtiques Universitat Jaume I.CastellóSpain Department Matemàtica Aplicada Universitat Politècnica de ValènciaSpain Department Matemàtica Aplicada Facultat MatemàtiquesUniversitat de ValènciaSpain

The simplest nMS flow is a polar flow formed by an attractive periodic orbit and a repulsive periodic orbit as limit sets. In this paper we show that the only orientable, simple, compact, 3-dimensional manifolds without boundary that admit an nMS flow with none or one saddle periodic orbit are lens spaces. We also see that when a fattened round handle is a connected sum of tori, the corresponding flow is also a trivial connected sum of flows.

关键词： nMS systems Links of periodic orbits Round handle decomposition Lens spaces

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Recent surface marine sediments of Cocos Island in Costa Rica

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International Journal of Sediment Research 2014年第1期29卷 59-72页

作者： Jeffrey A.SIBAJA-cordero Jesús S.TROnCOSO Eddy GóMEZ-RAMíREZ Escuela de Biología&Centro de Investigación en Ciencias del Mary Limnología (CIMAR) Universidad de Costa Rica Dr.grad.by Departamento de Ecología y Biología Animal Facultad de Ciencias del MarUniversidad de Vigo Departamento de Ecología y Biología Animal Facultad de Ciencias del MarUniversidad de VigoCampus Lagoas Marcosende Centro de Investigación en Ciencias del Mary Limnología(CIMAR) Universidad de Costa RicaSan Pedro 115012 060 San JoséCosta Rica Dr.(Cand).Departamento de Biología Facultad de Ciencias del Mary AmbientalesUniversidad de Cádiz

Subtidal sediments of Isla del Coco(Cocos Island),Costa Rica were described in their grain size,sorting,organic matter,and carbonates from 27 dredge samples(3-75 m),collected in April *** organic matter range between 1.37-3.31%and carbonates presented a mean of 74±17%.The sorting was moderately or *** grain size ranged between *** pattern is that sediment change from inner to mouth of *** and gravel fractions increased offshore and organic matter have high values inner the *** of vegetal debris and mud was from the rivers of this island covered with rain and cloud forest.

关键词： Sand bottom Sorting Organic matter Carbonates Isla del Coco

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