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To Analyze the Sensitivity of RT-PCR Assays Employing S Gene Target Failure with Whole Genome Sequencing Data during Third Wave by SARS-CoV-2 Omicron Variant

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Advances in Microbiology 2024年第5期14卷 247-255页

作者： Pooja Patel Yogita Mistry monika Patel Summaiya Mullan Viral Research and Diagnostic Laboratory Department of Microbiology Government Medical college Surat India

Introduction: Omicron is a highly divergent variant of concern (VOCs) of a severe acute respiratory syndrome SARS-CoV-2. It carries a high number of mutations in its spike protein hence;it is more transmissible in the community by immune evasion mechanisms. Due to mutation within S gene, most Omicron variants have reported S gene target failure (SGTF) with some commercially available PCR kits. Such diagnostic features can be used as markers to screen Omicron. However, Whole Genome Sequencing (WGS) is the only gold standard approach to confirm novel microorganisms at genetically level as similar mutations can also be found in other variants that are circulating at low frequencies worldwide. This Retrospective study is aimed to assess RT-PCR sensitivity in the detection of S gene target failure in comparison with whole genome sequencing to detect variants of Omicron. Methods: We have analysed retrospective data of SARS-CoV-2 positive RT-PCR samples for S gene target failure (SGTF) with TaqPath COVID-19 RT-PCR Combo Kit (ThermoFisher) and combined with sequencing technologies to study the emerged pattern of SARS-CoV-2 variants during third wave at the tertiary care centre, Surat. Results: From the first day of December 2021 till the end of February 2022, a total of 321,803 diagnostic RT-PCR tests for SARS-CoV-2 were performed, of which 20,566 positive cases were reported at our tertiary care centre with an average cumulative positivity of 6.39% over a period of three months. In the month of December 21 samples characterized by the SGTF (70/129) were suggestive of being infected by the Omicron variant and identified as Omicron (B.1.1.529 lineage) when sequence. In the month of January, we analysed a subset of samples (n = 618) with SGTF (24%) and without SGTF (76%) with Ct values Conclusions: During the COVID-19 pandemic, it took almost more than 15 days to diagnose infection and identify pathogen by sequencing technology. In contrast to that molecular assay provided qu

关键词： SARS-CoV-2 S Gene Target Failure Whole Genome Sequencing Omicron

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Harnessing immunity:Immunomodulatory therapies in COVID-19

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World Journal of Virology 2024年第2期13卷 34-47页

作者： Tsvetelina Velikova Hristo Valkov Anita Aleksandrova monika Peshevska-Sekulovska Metodija Sekulovski Russka Shumnalieva Medical Faculty Sofia University St.Kliment OhridskiSofia 1407Bulgaria Department of Gastroenterology University Hospital“Tsaritsa Yoanna-ISUL”Medical University of SofiaSofia 1527Bulgaria Department of Immunology SMDL RamusSimitli 6000Bulgaria Department of Gastroenterology University Hospital LozenetzSofia 1407Bulgaria Department of Anesthesiology and Intensive Care University Hospital LozenetzSofia 1407Bulgaria Department of Rheumatology Clinic of RheumatologyUniversity Hospital"St.Ivan Rilski"Medical University-SofiaSofia 1612Bulgaria

An overly exuberant immune response,characterized by a cytokine storm and uncontrolled inflammation,has been identified as a significant driver of severe coronavirus disease 2019(COVID-19)***,deciphering the intricacies of immune dysregulation in COVID-19 is imperative to identify specific targets for intervention and *** these delicate dynamics in mind,immunomodulatory therapies have emerged as a promising avenue for miti-gating the challenges posed by *** in manipulating immune pathways presents an opportunity to alter the host response,optimizing antiviral defenses while curbing deleterious *** review article compre-hensively analyzes immunomodulatory interventions in managing *** explore diverse approaches to mitigating the hyperactive immune response and its impact,from corticosteroids and non-steroidal drugs to targeted biologics,including anti-viral drugs,cytokine inhibitors,JAK inhibitors,convalescent plasma,monoclonal antibodies(mAbs)to severe acute respiratory syndrome coronavirus 2,cell-based therapies(i.e.,CAR T,etc.).By summarizing the current evidence,we aim to provide a clear roadmap for clinicians and researchers navigating the complex landscape of immunomodulation in COVID-19 *** Glucocorticoids are among the most widely prescribed drugs with their immune-suppressive and anti-inflammatory effect[84].The current guidelines for the treatment of COVID-19 recommend against the use of dexamethasone or other systemic CS in non-hospitalized patients in the absence of another indication[70].The RECOVERY trial demonstrates the reduced 28-d mortality among hospitalized patients with COVID-19 using dexamethasone compared to the usual standard of care,along with other investigators,such as Ahmed and Hassan[85].The benefit of dexamethasone was seen only among participants receiving either oxygen alone or invasive mechanical ventilation at randomization but not among those receiving no respi

关键词： Immunomodulation COVID-19 SARS-CoV-2 Immunotherapy Antiviral immune response Cytokine storm Adaptive immunity Therapeutic strategies Immune modulators Viral infection Host immune response

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The transcriptional landscape of the developmental switch from regular pollen maturation towards microspore-derived plant regeneration in barley

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The Crop Journal 2024年第4期12卷 1064-1080页

作者： Anna Nowicka Martin Kovacik Anna Maksylewicz Przemysław Kopec Ewa Dubas monika Krzewska Agnieszka Springer Robert E.Hoffie Diaaeldin S.Daghma Zbynek Milec Ales Pecinka Jochen Kumlehn Iwona Zur The Franciszek Górski Institute of Plant Physiology Polish Academy of Sciences Niezapominajek 2130-239 KrakówPoland Institute of Experimental Botany of the Czech Academy of Sciences Centre of Plant Structural and Functional GenomicsŠlechtitelu3177900 OlomoucCzech Republic Department of Cell Biology and Genetics Faculty of SciencePalacky UniversityŠlechtitelu°2777900 OlomoucCzech Republic Malopolska Centre of Biotechnology Jagiellonian University30-387 KrakówPoland Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)Gatersleben Corrensstraße 306466 Stadt SeelandGermany Institute for Breeding Research on Horticultural Crops Julius Kühn Institute(JKI)-Federal Research Centre for Cultivated PlantsErwin-Baur Straße 2706484 QuedlinburgGermany Global Change Research Institute Czech Academy of Sciences Beˇlidla 986/4a60300 BrnoCzech Republic

Plant formation from in vitro-cultivated microspores involves a complex network of internal and environmental ***/doubled haploids(DHs)derived from in vitro-cultured microspores are widely used in plant breeding and genetic ***,the mechanism underlying the developmental switch from regular pollen maturation towards microspore-derived plant regeneration remains poorly ***,RNA-sequencing was employed to elucidate the transcriptional landscapes of four early stages of microspore embryogenesis(ME)in barley cultivars Golden Promise and Igri,which exhibit contrasting responsiveness to microspore-derived plant *** experiments revealed fundamental regulatory networks,specific groups of genes,and transcription factor(TF)families potentially regulating the developmental *** identified a set of candidate genes crucial for genotype-dependent responsiveness/recalcitrance to *** high-resolution temporal transcriptome atlas provides an important resource for future functional studies on the genetic control of microspore developmental transition.

关键词： Hordeum vulgare Marker gene Microspore embryogenesis Stress response Transcriptome analysis

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Acute coalescent mastoiditis in a 16-month-old child due to Streptococcus pneumoniae infection

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Journal of Acute Disease 2024年第1期13卷 36-39页

作者： Magdalena Pszczołowska monika Morawska-Kochman Katarzyna Resler Katarzyna Pękalska Tomasz Zatoński Department and Clinic of Otolaryngology Head and Neck SurgeryWrocław Medical UniversityBorowska 213WrocławPoland Department of General Radiology Interventional Radiology and NeuroradiologyWrocław Medical UniversityBorowska 213WrocławPoland

Rationale:Acute otitis media is a common disease in early childhood,and is usually caused by Streptococcus pneumoniae(***).Acute mastoiditis is a complication of acute otitis media and can involve not only the mucoperiosteum of the middle ear but can also spread to the periosteum by destroying the mastoid bone(acute coalescent mastoiditis).In addition,the infection can extend through the surrounding bones or the emissary veins beyond the mastoid’s air cells,leading to subperiosteal ***’s Concern:A 16-month-old female patient was hospitalized due to the purulent discharge of the left ear and the symptoms of right mastoiditis(swelling and redness of the skin).Diagnosis:Bilateral acute coalescent mastoiditis caused by *** *** computer tomography revealed bilateral bone destruction of the mastoid and abscesses found behind the auricle on both ***:The patient underwent intravenous antibiotic therapy and surgical ***:The patient was discharged 14 days after hospitalization with an improved ***:Improperly treated acute coalescent mastoiditis can lead to extracranial and intracranial complications,sometimes serious and even *** are prevalent in children under 2 years,in whom the disease progresses more rapidly and *** vaccination with a 13-valent vaccine may not result in sufficient immunity against ***,a predominant pathogen in children affected by acute coalescent mastoiditis.

关键词： Acute otitis media Acute coalescent mastoiditis Streptococcus pneumoniae Children Case report

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Low-grade myofibrosarcoma of the maxillary sinus:Two case reports

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World Journal of Clinical Oncology 2024年第4期15卷 566-575页

作者： Anna Mydlak ŁukaszŚcibik monika Durzynska Jakub Zwoliński Karolina Buchajska Olga Lenartowicz Jakub Kucharz Department of Head and Neck Cancer Maria Sklodowska-Curie National Research Institute of OncologyWarsaw 02-781Poland Department of Otolaryngology and Head and Neck Oncological Surgery The 5th Military Clinical Hospital with PolyclinicKrakow 30-901Poland Department of Pathology Maria Sklodowska-Curie National Research Institute of OncologyWarsaw 02-781Poland Department of Genitourinary Oncology Maria Sklodowska-Curie National Research Institute of OncologyWarsaw 02-781Poland

BACKGROUND Low-grade myofibroblastic sarcoma(LGMS)is an extremely rare tumor characterized by the malignant proliferation of *** most commonly develops in adults,predominantly in males,in the head and neck region,oral cavity,especially on the tongue,mandible,and *** article presents 2 cases of LGMS localized to the maxillary sinus and provides an overview of the available *** SUMMARY Two patients with LGMS located in the maxillary sinus underwent surgery at the Department of Head and Neck *** 1:A 46-year-old patient was admitted to the clinic with suspected LGMS recurrence in the right maxillary sinus(rT4aN0M0),with symptoms of pain in the suborbital area,watering of the right eye,thick discharge from the right nostril,and augmented facial *** open biopsy-confirmed LGMS,the patient underwent expanded maxillectomy of the right side with immediate palate reconstruction using a microvascular skin flap harvested surgically from the middle *** patient qualified for adjuvant radiotherapy for the postoperative bed,with an additional ***,the patient is under 1.5 years of observation with no evidence of *** 2:A 45-year-old man was admitted to our clinic with facial asymmetry,strabismus,exophthalmos,and visual impairment in the right *** months earlier,the patient had undergone partial jaw resection at another hospital for fibromatosis.A contrast-enhanced computed tomography scan revealed a tumor mass in the postoperative log after an earlier *** open biopsy confirmed lowgrade fibrosarcoma(rT4aN0M0).The patient qualified for an extended total right maxillectomy with orbital excision and right hemimandibulectomy with immediate microvascular reconstruction using an anterolateral thigh *** patient subsequently underwent adjuvant radiotherapy to the postoperative *** 9 months,recurrence occurred in the right mandibular arch below the irradiated *** lesion infiltrated t

关键词： Head and neck cancer Paranasal sinuses Maxillary sinus Sarcoma Low-grade myofibroblastic sarcoma Case report

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Chinese Herb Formulae Inhibit the Proliferation of Human Colon Cancer SW480 Cells by Inducing Cell Apoptosis

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World Journal of Traditional Chinese Medicine 2023年第3期9卷 348-354页

作者： Su Fu Shao-Lin Qin Wolf-Dieter rausch Department for Internal Medicine of Traditional Chinese Medicine Eye Hospital China Academy of Chinese Medical SciencesBeijingChina Department for Biomedical Sciences Institute for Chemistry and BiochemistryUniversity of Veterinary Medicine ViennaViennaAustria

Objective:This study aimed to reveal the antitumor effects of Chinese herbal formulae and the underlying mechanisms in treating colorectal cancer,with a focus on developing traditional Chinese medicine(TCM)as a supplement and alternative therapeutic method for *** and Methods:Human colon cancer SW480 cells were treated with three Chinese herbal formulae,Bu Zhong Yi Qi Decoction,Fuzi Lizhong Decoction,and Pulsatilla Decoction at different concentrations(50–600μg/mL)for 24,36,and 48 h,*** viability was determined using the resazurin reduction assay,and cell survival rate was evaluated using a colony formation *** treatment with different concentrations(50–600μg/mL)of these three formulae for 48 h,the effects of the Chinese herbal formulae on cell apoptosis were investigated using Hoechst/propidium iodide(PI)*** positive PI-stained cells were investigated using an EnSpire multilabel plate reader and the positive Hoechst-stained cells were observed under a fluorescence microscope for morphological ***:Bu Zhong Yi Qi Decoction,Fuzi Lizhong Decoction,and Pulsatilla Decoction inhibited SW480 cell proliferation in a dose-and time-dependent manner and induced cell ***:Chinese herbal formulae with a special prescription form of TCM with antitumor effects bring a new perspective in line with the principles of TCM in cancer treatment.

关键词： Apoptosis cell proliferation Chinese herbal formulae colorectal cancer SW480 cells

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Optimization of growth medium for microbially induced calcium carbonate precipitation(MICP) treatment of desert sand

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Journal of Arid Land 2023年第7期15卷 797-811页

作者： monika DAGLIYA Neelima SATYAM Ankit GARG Department of Civil Engineering Indian Institute of TechnologyIndore 453552India Guangdong Engineering Center for Structure Safety and Health Monitoring Shantou UniversityShantou 515063China

Wind-induced sand erosion is a natural process, and can have several negative impacts on human health, environment, and economy. To mitigate the wind-induced sand erosion, an environmental friendly technique that helps to bind soil particles is desirable. The microbially induced calcium carbonate precipitation(MICP) treatment has lately become renowned and a viable alternative to enhance the binding of sand particles(especially against wind erosion). The efficiency of Sporosarcina pasteurii bacteria in inducing calcite formation can be influenced by various factors, including the type of growth media used for bacterial culture. Most of the studies have mainly validated the efficiency of S. pasteurii bacteria usually under single growth media for the MICP treatment. However, the efficiency of S. pasteurii under different growth media on calcite formation is rarely explored. The current study explores the effect of *** bacteria on calcite formation under the presence of three different growth media, namely,molasses(MS), tryptic soy broth(TB), and nutrient broth(NB). The three growth media have been applied in the laboratory with and without bacterial solution(control samples). Altered cementation media concentrations(0.5 and 1.0 M) with different pore volumes(PVs), namely, 0.25, 0.50, and 1.00 PV were used in sand-filled tubes for 7 and 14 treatment cycles(1 cycle=24 h). The pH and EC were measured for 12-h period in every 2 h interval, to monitor values at the time of treatment at room temperature. The calcite precipitation was confirmed using SEM(scanning electron microscope), PXRD(powder X-ray diffraction), and calcimeter tests. It was observed that MS generates lower calcite precipitation as compared with NB and TB. However, MS has the advantage of being more economical and abundant(waste product from sugar mills and refineries) as compared with other growth media(NB and TB). It was observed that the minimum and the maximum calcite precipitation using MS i

关键词： growth media molasses tryptic soy broth nutrient broth S.pasteurii calcium carbonate

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A small number of male-biased candidate pheromone receptors are expressed in large subsets of the olfactory sensory neurons in the antennae of drones from the European honey bee Apis mellifera

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Insect Science 2022年第3期29卷 749-766页

作者： Joerg Fleischer Alexander rausch Kathrin Dietze Silvio Erler Sina Cassau Jurgen Krieger Martin Luther University Halle-Wittenberg Institute of Biologyl ZoologyDepartment of Animal PhysiologyHalle(Saale) Institute for Bee Protection Julius Kuhn Institute(JKI)-Federal Research Centre for Cultivated PlantsBraunschweigGermany

In the European honey bee(Apis mellifera),the olfactory system is essential for foraging and intraspecific communication via *** bees are equipped with a large repertoire of olfactory receptors belonging to the insect odorant receptor(OR)*** studies have indicated that the transcription level of a few OR types including OR11,a receptor activated by the queen-released pheromone compound(2£)-9-oxodecenoic acid(9-ODA),is significantly higher in the antenna of males(drones)than in female ***,the number and distribution of antennal cells expressing male-biased ORs is ***,we analyzed antennal sections from bees by in situ hybridization for the expression of the male-biased receptors OR11,OR18,and *** results demonstrate that these receptors are expressed in only moderate numbers of cells in the antennae of females(workers and queens),whereas substantially higher cell numbers express these ORs in ***,the reported male-biased transcript levels are due to sex-specific differences in the number of antennal cells expressing these *** analyses for OR11 and OR18 in drone antennae revealed expression in two distinct subsets of olfactory sensory neurons(OSNs)that in total account for approximately 69%of the OR-positive *** high percentages of OSNs expressing given receptors are reminiscent of male-biased ORs in moths that mediate the detection of female-released sex pheromone ***,our findings indicate remarkable similarities between male antennae of bees and moths and support the concept that male-biased ORs in bee drones serve the detection of female-emitted sex pheromones.

关键词： chemosensory odorant receptor olfaction pheromone detection sensilla placodea

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DeepWalk Based Influence Maximization (DWIM): Influence Maximization Using Deep Learning

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Intelligent Automation & Soft Computing 2023年第1期35卷 1087-1101页

作者： Sonia Kapil Sharma monika Bajaj Department of Information Technology Delhi Technological UniversityNew Delhi110039India Department of Computer Science University of DelhiNew Delhi110007India

Big Data and artificial intelligence are used to transform *** networking sites have given a new dimension to online *** media platforms help gather massive amounts of data to reach a wide variety of customers using influence maximization technique for innovative ideas,products and *** paper aims to develop a deep learning method that can identify the influential users in a *** method combines the various aspects of a user into a single *** a social network,the most influential user is the most trusted *** significant users are used for viral marketing as the seeds to influence other users in the *** proposed method combines both topical and topological aspects of a user in the network using *** proposed method is DeepWalk based Influence Maximization(DWIM).The proposed method was able tofind k influential nodes with computable time using the *** experiments are performed to assess the proposed algorithm,and centrality measures are used to compare the *** results reveal its performance that the proposed method canfind k influential nodes in computable *** can identify influential users,which helps viral marketing,outlier detection,and recommendations for different products and *** applying the proposed methodology,the set of seed nodes gives maximum influence measured with respect to different centrality measures in an increased computable time.

关键词： Deep learning inﬂuence maximization graph embedding deepwalk

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Microbiome therapeutics:exploring the present scenario and challenges

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Gastroenterology Report 2022年第1期10卷 18-36页

作者： monika Yadav Nar Singh Chauhan Department of Biochemistry Maharshi Dayanand UniversityRohtakHaryanaIndia

Human gut-microbiome explorations have enriched our understanding ofmicrobial colonization,maturation,and dysbiosis in health-and-disease *** enormousmetabolic potential of gutmicrobes and their role in themaintenance of human health is emerging,with new avenues to use themas therapeutic agents to overcome human *** therapeutics are aimed at engineering the gutmicrobiome using additive,subtractive,ormodulatory therapy with an application of native or engineeredmicrobes,antibiotics,bacteriophages,and *** approach could overcome the limitation of conventional therapeutics by providing personalized,harmonized,reliable,and sustainable *** huge economic potential has been shown in the global *** the therapeutic and economical potential,microbiome therapeutics is still in the developing stage and is facing various technical and administrative issues that require research *** review aims to address the current knowledge and landscape ofmicrobiome therapeutics,provides an overview of existing health-and-disease applications,and discusses the potential future directions ofmicrobiomemodulations.

关键词： microbiome therapeutics human microbiome engineered microbiome probiotics prebiotics fecal microbiome transplantation

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