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The Response of Cockerel Semen Kinematic Parameters LIN, STR, WOB, ALH and BCF to Induced Oxidative Stress

Open Journal of Animal Sciences 2021年第2期11卷 292-303页

作者： Cyril Mpho Pilane mohleko helen mapeka Agricultural Research Council Animal Production Campus Germplasm Conservation & Reproductive Biotechnologies Irene South Africa Department of Animal Production and Agricultural Economics Faculty of Science and Agriculture University of Limpopo Sovenga South Africa

Computer-assisted sperm class analyser (CASA) analysis of avian semen following cryopreservation indicates that their semen motility and viability parameters become compromised, due in part to oxidative stress. To mimic these observations we have treated cockerel semen with an oxidative stress inducing agent, namely hydrogen peroxide (H2O2) and monitored the motility, kinematic and viability parameters over time. Briefly, five healthy and fertile South African Venda cockerels were selected and their semen was collected using the abdominal massage technique. The semen was then treated with H2O2 at 0 µM, 5 µM, 50 µM and 200 µM concentrations for 0, 3, 16 and 24 hrs. The semen motility, kinematic and viability parameters were then determined using the CASA system while the viability was determined using the SYBR-14/PI staining. The Pearson’s correlation coefficient was determined to test the relationships between the levels of induced oxidative stress, period of exposure to oxidative stress inducing agent and the motility plus kinematic parameters. Our data revealed that in raw cockerel semen, there was high and positive correlations between total motility (TM), progressive motility (PM), rapid (RAP), curvilinear velocity (VCL), straight line velocity (VSL) and average path velocity (VAP) while the kinematic parameters LIN, STR, WOB, ALH and BCF had low or negative correlations with them. Furthermore, TM, PM, RAP, VCL and VSL remained highly and positively correlated with the induced oxidative stress and also, linearity (LIN), straightness (STR), wobble (WOB), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) remained negatively correlated with the induced oxidative stress, after 3 hrs. After 24 hrs, TM, PM, RAP, VCL, VSL, VAP and ALH, became negatively correlated with the induced oxidative stress while LIN, STR, WOB and BCF became positively correlated with the induced oxidative

关键词： Cockerel Oxidative Stress Kinematic Parameters Semen

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Buck Semen Does Not Easily Succumb to Oxidative Stress

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Open Journal of Animal Sciences 2019年第1期9卷 65-75页

作者： Cyril Mpho Pilane Malebogo Audrey Bopape Bovula Ntombizodwa mohleko helen mapeka Agricultural Research Council Animal Production Campus Germplasm Conservation & Reproductive Biotechnologies Irene South Africa Department of Biological and Agricultural Sciences Sol Plaatjie University Kimberly South Africa Department of Agricultural Earth and Environmental Science Animal and Poultry Science University of KwaZulu-Natal Scottsville South Africa

Semen processing and manipulation generally result in loss of sperm motility and sperm velocity due in part to oxidative stress. In this study we investigated the vulnerability of South African indigenous unimproved buck semen to oxidative stress induced by an oxidative stress inducing agent, namely, hydrogen peroxide (H2O2). Semen ejaculates were collected from four superior South African indigenous unimproved bucks in a total of ten collections and then each duplicate was treated with different concentrations of H2O2 in presence or absence of Dithiothreitol (DTT). Sperm motility and velocities were determined using the computer aided sperm class analyser (CASA). SYBR-14 and propidium iodide (PI) Live/Dead assay kit was used to determine cell viability and Yo-Pro-1 plus PI Apoptosis kit was used to determine apoptosis. Statistical analysis was performed on the data using SPSS version 17.0 for Windows (SPSS Inc., Chicago, IL). South African indigenous unimproved buck raw semen motility was between 97% with 98% viability and 0% apoptotic cells. Comparisons of the untreated controls at 0 and 3 hrs incubations revealed that after 3 hrs there was overall a decrease in the number viable cells with the majority of remaining cells exhibiting circular movements accompanied by high progressive (PM) and rapid (RAP) motilities. In treated South African indigenous unimproved buck semen, H2O2 marginally increased total motility (TM) with few apoptotic sperm cells while retaining high viability. Also, H2O2 increased straight line distances travelled of more than 4 fold as compared to untreated controls with no circularly moving cells. Moreover, inclusion of DTT, an antioxidant, had minimal effects on TM, RAP, curvilinear velocity (VCL), straight line velocity (VSL), linearity (LIN) and wobble (WOB) but positively affected PM, average path velocities (VAP), apoptosis and viability. Our Pearson’s correlation data revealed that only straightness (STR) was highly positively affected

关键词： South African Indigenous Bucks Oxidative Stress Motility Semen

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Assessment of the Susceptibility of Boar Semen to Oxidative Stress

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Open Journal of Animal Sciences 2016年第2期6卷 123-130页

作者： Cyril Mpho Pilane Malebogo Audrey Bopape mohleko helen mapeka Thivhilaheli Richard Netshirovha Agricultural Research Council Animal Production Institute Germplasm Conservation & Reproductive Biotechnologies Pretoria South Africa Department of Animal Science Faculty of Science Tshwane University of Technology Pretoria South Africa Faculty of Health and Environmental Science School of Agriculture and Environmental Science Central University of Technology Bloemfontein South Africa

The present study was conducted to assess boar sperm susceptibility to oxidative stress generated by hydrogen peroxide (H2O2). Semen was collected in replicates from three experimental large white boars using the gloved-hand technique. Semen ejaculates from three boars were treated with different concentrations of H2O2 for three hours. SYBR-14 and Propidium Iodide (PI) Live/ Dead assay kit was used to determine cell viability, and Yo-pro-1 and PI apoptosis kit was used to determine cell death, namely, apoptosis. Boar sperm motility obtained using computer aided sperm analysis (CASA) was between 90% and 100% with more than 98% viability with 0% apoptotic cells. In H2O2 treated boar sperm cells, rapid (RAP) and progressive motility (PM) increased. Also, H2O2 treatment induced a high positive correlation with apoptosis but high negative correlation with viability. Hydrogen peroxide decreased boar semen total motility (TM) by 10%. In addition, most of the boar sperm cells became apoptotic and lost 55% of viability under oxidative stress induced by H2O2. This study illustrated that boar semen was more susceptible to oxidative stress induced by H2O2.

关键词： Oxidative Stress Motility Apoptosis Viability Semen

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