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Lycopene modulates cellular proliferation, glycolysis and hepatic ultrastructure during hepatocellular carcinoma

World Journal of Hepatology 2016年第29期8卷 1222-1233页

作者： Prachi Gupta Nisha Bhatia mohinder pal bansal Ashwani Koul Department of Biophysics Basic Medical Sciences Block Ⅱ Panjab University

AIMTo investigate the effect of lycopene extracted from tomatoes (LycT) on ultrastructure, glycolytic enzymes, cell proliferation markers and hypoxia during N-Nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis. METHODSFemale BALB/c mice were randomly divided into four groups: The Control, NDEA (200 mg NDEA/kg b.w. given i.p.), LycT (5 mg/kg b.w. given orally on alternate days) and LycT + NDEA group. The mRNA and protein expression of various cell proliferation markers (PCNA, Cyclin D1, and p21) were assessed by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay, respectively. The ultrastructure of hepatic tissue was analyzed using scanning and transmission electron microscopy. The enzymatic activity of glycolytic enzymes was estimated using standardized protocols, while glucose-6-phosphate dehydrogenase activity level was estimated using a kit obtained from Reckon Diagnostic P. Ltd. (India). RESULTSUncontrolled proliferation in the liver of NDEA (P ≤ 0.001) mice was evident from the high expression of cell-proliferation associated genes (PCNA, Cyclin D1, and p21) when compared to control and LycT mice. In addition, enhanced activities of hexokinase, phosphoglucoisomerase, aldolase, glucose-6-phosphate dehydrogenase and hypoxia-inducible factor-1α were observed in NDEA mice as compared to control (P ≤ 0.001) and LycT (P ≤ 0.001) mice. The alterations in hepatic ultrastructure observed in the NDEA group correlated with the changes in the above parameters. LycT pre-treatment in NDEA-challenged mice ameliorated the investigated pathways disrupted by NDEA treatment. Moreover, hepatic electron micrographs from the LycT + NDEA group showed increased macrophages, apoptotic bodies and well-differentiated hepatocellular carcinoma (HCC) in comparison to undifferentiated HCC as observed in the NDEA treated group. CONCLUSIONThis study demonstrates that dietary supplementation with LycT has a multidimensional role in preventing HCC de

关键词： Hepatocellular carcinoma Ultrastructure Hypoxia Cell proliferation Lycopene Glycolysis

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Comparative quantitative analysis of fruit oil from Hippophae rhamnoides(seabuckthorn) by qNMR, FTIR and GC–MS

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Chinese Herbal Medicines 2023年第4期15卷 607-613页

作者： Dattatraya Dinkar Gore Furkan Ahmad Kulbhushan Tikoo Arvind Kumar bansal Dinesh Kumar Inder pal Singh National Institute of Pharmaceutical Education and Research(NIPER) S.A.S.Nagar 160062India CSIR-Institute of Himalayan Bioresource and Technology Palampur 176061India

Objective: To develop a qNMR method for quantitative analysis of triacylglycerols in fruit oil of Hippophae rhamnoides(seabuckthorn, SBT) and analyze commercial samples of SBT oils using GC–MS and ***: SBT fruit oil(IPHRFH) was extracted with hexane and the triglyceride(TAG) was isolated by vacuum liquid chromatography. Six different branded SBT oils purchased from e-commerce suppliers(Amazon) and in-house prepared SBT oil was analyzed by qNMR and fatty acyl composition of TAGs determined by using NMR. In-house oil was also analysed by GC–MS and FTIR ***: The qNMR results showed that the oil contained 80.3% of triacylglycerol(TAG). The SBT oil TAGs comprised of linolenate 6.6%, palmitoleate/oleate 65.4%, and total saturated fatty acyl chain including palmitate 28% as determined by qNMR. GC–MS analysis revealed that the major acyl functionalities present in the TAG were palmitoleic acid 36.5%, oleic acid 12.9%, palmitic acid 21.2%, and linoleic acid 18%. Of the six commercial samples analyzed, samples from only one supplier(SW) were fruit oil;All others were the seed oils or mix of fruit oil and seed oil. The labels for samples except for the SW did not indicate whether it was fruit oil or seed ***: The results suggest that SBT oil should be analyzed by combination of GC–MS, FTIR and qNMR for factual content of free fatty acid or TAGs, which are chemically different in nature and affect the quality of oil. GC–MS showed the content of omega free fatty acids after hydrolysis, while qNMR and FTIR showed the content of TAGs. The major acyl functionalities found in SBT fruit oil TAGs are palmitoleate/palmitate/oleate, while linoleate and linonelate make up a minor fraction. Furthermore,analysis of commercial samples showed discrepancies between label claims and actual content.

关键词： Hippophae rhamnoides L. omega fatty acids qNMR seabuckthorn triacylglycerols

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杂种桉人工能源林的生长生物产量和干物质分配格式

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广东林业科技 1992年第4期8卷 44-48页

作者： mohinder pal 曾天勋

杂种桉(Eucalyptus hybrid)是印度营造人工林较喜爱的树种之一.它生长迅速,适应于不同的气候和土壤条件,且用途广。杂种桉的生物量使其成为印度许多社会林业计划的主要树种。本文研究在半干旱地区供水条件下,人工能源林中的杂种桉性状,... 详细信息

杂种桉(Eucalyptus hybrid)是印度营造人工林较喜爱的树种之一.它生长迅速,适应于不同的气候和土壤条件,且用途广。杂种桉的生物量使其成为印度许多社会林业计划的主要树种。本文研究在半干旱地区供水条件下,人工能源林中的杂种桉性状,特别着重杂种桉生物量在数量和质量上的研究,有机质分配格式和生物量的不同产出。

关键词：杂种桉生长生物量干物质

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Ivabradine—The Final Crusader for Postoperative Junctional Ectopic Tachycardia, a Case Report with Literature Review

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World Journal of Cardiovascular Surgery 2019年第8期9卷 73-82页

作者： Manoj Kumar Sahu Harsha Vardhan Niraghatam Nikhil bansal Sarvesh pal Singh palleti Rajashekar Shiv Kumar Choudhary Department of Cardiothoracic and Vascular Surgery CN Centre All India Institute of Medical Sciences New Delhi India

Background: Junctional ectopic tachycardia (JET) is one troublesome supraventricular arrhythmia in postoperative pediatric cardiac surgical patients. Unless treated timely and effectively it may lead to morbidity and even mortality. Aim: To understand the role of Ivabradine in the treatment of JET in postoperative pediatric cardiac surgical patients. Case: We present a postoperative case of complete repair of Tetralogy of Fallot who was in normal sinus rhythm in the preoperative period and developed JET 4 hours later in postoperative period which was persistent despite measures to optimize the internal milieu of the body and conventional antiarrhythmics, that was successfully treated with Ivabradine at a dose of 0.05 mg/kg/12 hourly. Conclusion: Ivabradine can be used to successfully treat postoperative JET in cases ofrefractory to other antiarrhythmic drugs like Digoxin and Amiodarone.

关键词： Tetralogy of Fallot Junctional Ectopic Tachycardia Ivabradine

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