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Liposomes as immunological adjuvants and delivery systems in the development of tuberculosis vaccine:A review

Asian Pacific Journal of Tropical Medicine 2022年第1期15卷 7-16页

作者： Nur Ellene Mat Luwi Suhana Ahmad Ahmad Suhaimi Nurfatihah Azlyna Asyikin nordin Maria Elena Sarmiento Armando Acosta mohd nor norazmi Vuk Uskoković Rohimah Mohamud Ramlah Kadir Department of Immunology School of Medical SciencesUniversiti Sains Malaysia16150 Kubang KerianKelantanMalaysia School of Health Sciences Universiti Sains Malaysia16150 Kubang KerianKelantanMalaysia Department of Mechanical Engineering San Diego State UniversityCampanile Dr.5500San Diego 921982CAUSA TardigradeNano LLC IrvineCA 92604USA

Liposomes are phospholipid bilayer vesicles,which are biocompatible,biodegradable and nontoxic vehicles suitable for numerous drug and gene delivery *** this review,we discuss the prospect of using liposome technology in the development of a vaccine for *** remains an important health problem that requires the development of an effective vaccine,especially since the only approved vaccine for it continues to be the Bacille Calmette-Geurin(BCG)one developed 100 years *** review focuses on the different applications of liposomes toward achieving this *** liposomal formulations showing prospect in the research stage and in clinical trials are discussed.

关键词： Liposomes Tuberculosis vaccine Adjuvant Delivery system Tuberculosis

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Specific and cross-reactive immune response against Mycobacterium tuberculosis antigens in mice immunized with proteoliposomes from Mycobacterium bovis BCG

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Asian Pacific Journal of Tropical Biomedicine 2017年第3期7卷 188-192页

作者： Nadine Alvarez DaymíSerpa Ramlah Kadir Yanely Tirado Reinier Borrero Sonsire Fernández Rubén Cabrera Yolanda Valdes Caridad Zayas Reinaldo Acevedo Luis Izquierdo María Elena Sarmiento mohd-nor norazmi JoséLuis Pérez Armando Acosta Finlay Institute School of Health Sciences University Sains Malaysia Institute for Research in Molecular Medicine University Sains Malaysia

Objective: To characterize the immunogenicity and the induction of cross-reactive responses against Mycobacterium tuberculosis(M. tuberculosis) of a proteoliposome(PL)from Mycobacterium bovis Bacillus Calmette–Guerin(BCG) with and without alum hydroxide(AL) as adjuvant(PLBCG-AL and PLBCG, respectively) in BALB/c ***: BALB/c mice were inoculated with phosphate buffer solution, BCG, PLBCG and PLBCG-AL. The humoral immunogenicity was determined by ELISA [immunoglobulin G(Ig G), Ig G1 and Ig G2a] and the cellular immunogenicity was evaluated in vivo by delayed type hypersensitivity. The humoral cross-reactive response against M. tuberculosis was determined by Western ***: Sera from animals immunized with PLBCG-AL and PLBCG showed significant increase in specific total Ig G and Ig G1 antibodies and the presence of cross-reactive antibodies against M. tuberculosis antigens, which were more intense with the use of alum as adjuvant. Mice immunized with PLBCG and PLBCG-AL also showed a specific cellular response in ***: The cellular and humoral immunogenicity of PLBCG and the capacity to induce cross-reactive responses against M. tuberculosis is in agreement with the protective capacity previously demonstrated by this vaccine candidate and supports the continuation of its evaluation in further stages.

关键词： BCG Immunogenicity Mycobacterium tuberculosis Proteoliposomes

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Role of toll like-receptor 2 in inflammatory activity of macrophage infected with a recombinant BCG expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum

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Asian Pacific Journal of Tropical Biomedicine 2018年第7期8卷 333-339页

作者： nor Munirah Zakaria Rapeah Suppian norazmi mohd nor nor Fazila Che Mat School of Health Sciences Health Campus Universiti Sains Malaysia

Objective: To investigate the role of toll-like receptor 2(TLR2) in inflammatory activity of macrophage infected with the recombinant Mycobacterium bovis bacillus Calmette-Guerin(rBCG). Methods: Mouse macrophage cell line J774 A.1 was infected with Mycobacterium bovis bacillus Calmette-Guerin(BCG) and rBCG cultures for 48 h in the presence or absence of 10 μg/mL of TLR2 inhibitor. Untreated macrophages were used as a negative control while lipopolysaccharide-stimulated macrophages were used as a positive control. The ability of the macrophage to engulf the BCG and rBCG in the absence or presence of TLR2 inhibitor was assessed using a phagocytic assay, while the production of inflammatory cytokines and nitric oxide by the infected macrophages was evaluated using ELISA and Griess reagent method, while the expression of the inducible nitric oxide synthase was determined using Western blot analysis. Results: The results showed that blocking TLR2 function reduced the phagocytic activity, nitric oxide production and proinflammatory cytokine secretion such as TNF-α, IL-1β and IL-12 p40 as well as inducible nitric oxide synthase expression in the infected macrophages. These data showed the importance of TLR2 in the activation of macrophages following BCG and r BCG infections. Conclusions: Through exploring the immunological mechanism which underlies the protection conferred by the candidate vaccine, this study will improve our understanding of the vaccine candidate's mechanism to protect the host from malaria infection.

关键词： Macrophages Mycobacterium bovis bacillus Calmette-Guerin Plasmodium falciparum Proinflammatory cytokine Toll-like receptor 2

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