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Validation of a Method for Characterization of Ethanol in Water by HS-GC-FID to Serve the Traceability of Halal Measurements

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Green and Sustainable Chemistry 2024年第2期14卷 17-28页

作者： Adel B. Shehata Abdulrahman R. Al Askar mohammed A. Al rasheed Abdulrahman M. Al Zahrany Fahd A. Al Kharraa Sowailem A. Al Sowailem Chemistry Department/National Measurement and Calibration Center (NMCC) Saudi Standards Metrology and Quality Organization (SASO) Riyadh Kingdom of Saudi Arabia

The determination of the ethanol content in food products is of fundamental importance for HALAL certification. In this work, an analytical method for the determination of ethanol in water by headspace gas chromatography with flame ionization detector (HS-GC-FID) has been developed and validated for the use in characterization of ethanol reference materials. The validation study was carried out in the linear calibration range 100 - 1500 mg/kg using the NIST SRM 2900, nominal 95.6%. The studied performance characteristics of the method were the limit of detection, LOD, the limit of quantification LOQ, selectivity, linearity, precision, recovery and bias. The validation results showed that the method is selective, precise, accurate and free from any significant bias. The LOD and LOQ were 1.27 and 3.86 mg/kg respectively and the estimated expanded uncertainty was 2% indicating that the method is fit for the purpose of certification of ethanol in water reference materials.

关键词： Ethanol HS-GC-FID Validation Linearity Precision Bias

Portraying Diabetic Foot Ulcers: Comparative Evaluation of Diabetic Foot Infections versus Diabetic Foot Ulcers

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Advances in Infectious Diseases 2024年第2期14卷 297-309页

作者： Jamal Wadi Al Ramahi Leen Sharqawi Sarah AL-Najafi Eman Awwad Haya M. Al-Obaidi Abdel Hafez Mohammad Sara Nofal Dina Riyadh Al-Janabi Ibrahim Zuhair Hamza Jamal Al-Masaeid mohammed Al-Ithawi Dina rasheed Bellal O. Al-Far Osama Al-Izi Qutaiba Al-Bustanji School of Medicine The University of Jordan Amman Jordan Department of the Internal Medicine The Specialty Hospital Amman Jordan Department of Pharmacy The Specialty Hospital Amman Jordan Department of the Medical Al Khalidi Hospital Amman Jordan Department of Pharmacy Jordan Hospital Amman Jordan Department of Internal Medicine Jordan Hospital Amman Jordan Department of General Surgery Jordan Hospital Amman Jordan

Background: Confusion often arises in caring for diabetic foot infections and ulcers, especially with antimicrobials;we aim to shed light on this entity and alert healthcare workers to its stewardship. Methods: Records were reviewed between February 2016 and September 2023. Data for patients diagnosed with diabetes and foot ulcers, infected or not, were examined following ICD 9 search terms. Records for patients were included if they were prediabetic/diabetic adults with foot ulcers, more than 18 years old, and on antidiabetic treatment. Patients were excluded if they insulin resistant, with normal HgbA1c levels, wheel-chair dependent, bed-bound, non-diabetic patients, diabetic patients who had vascular lower limb surgery earlier to ulcers, diabetic patients who had aortocoronary bypass, deep venous thrombosis within six months, malignancy, and severe clinical depression. A modified IWGDF/IDSA guidelines definitions for DFI and DFU was considered. Statistical analysis was done using R programming. Statistical methods were employed as appropriate, and a significant P-value was considered for P Results: Most characteristics were well balanced between DFI and DFU, on imaging osteomyelitis and tissue swelling were significantly more in DFI. Endovascular radiological procedures showed angiograms to be considerably more in DFI, while angioplasty was more in DFU, in addition to smoking. Bacteremia was uncommon, and swab cultures were mostly polymicrobial in both ulcers;no clear association with blood bacteria was detected with the polymicrobial growth, though few were concordant. Antimicrobials prescribed for both ulcers were not statistically different except for carbapenems, which were more in DFI (P Conclusion: Attention should be paid to best practices while caring for diabetic ulcers. These include swab culture interpretations, the use of antimicrobials, and plan management according to DFI or DFU to utilize either local care or combination with antimicrobials.

关键词： Diabetic Foot Ulcers Diabetic Limb Amputation Antimicrobials Stewardship Broad-Spectrum Antimicrobials in Diabetic Foot Ulcers

Bacteria as indicators for finding oil and gas reservoirs:A case study of the Bikaner-Nagaur Basin,Rajasthan,India

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Petroleum Science 2011年第3期8卷 264-268页

作者： mohammed Abdul rasheed Mutnuri Lakshmi Deshoju Srinu Anurodh Mohan Dayal Microbiology Laboratory Petroleum Geochemistry DivisionNational Geophysical Research InstituteCouncil for Scientific and Industrial Research (CSIR)Uppal RoadHyderabad-500606India

Geo-microbial prospecting for hydrocarbons is an exploration method based on the seepage of light gaseous hydrocarbons from oil/gas reservoirs to the surface and their utilization by hydrocarbon oxidizing bacteria. These bacteria utilize hydrocarbon gases as their sole source of food and are found to be enriched in the near surface soils/sediments above the oil and gas reservoirs. The detection of anomalous populations of n-pentane and n-hexane oxidizing bacteria in the surface soils can help to evaluate the prospects for hydrocarbon exploration. A geo-microbial survey has been carried out in the Bikaner Nagaur basin to investigate the prospects for hydrocarbon exploration. In the present study, bacterial counts for n-pentane utilizing bacteria range between 2.0× 10^2 and 1.26× 10^6 cfu/gm and n-hexane utilizing bacteria range between 2.0× 10^2 and 1.21 × 10^6 cfu/gm. The bacterial concentration distribution maps show four distinct anomalies in the study area. The possibility of discovering oil or gas reservoirs using the microbiological method is emphasized by the tact that the hydrocarbon-oxidizing bacteria range between 103 and 106 cfu/gm in soil/sediment receiving hydrocarbon micro-seepages. In the present study area of the Bikaner Nagaur basin, n-pentane and n-hexane utilizing bacteria are found between 105 and 106 cfu/gm of soil sample, which is significant and thereby substantiates the seepage of lighter hydrocarbon accumulations from oil and gas reservoirs. Geo-microbial prospecting studies suggest that hydrocarbon micro-seepage of subsurface origin is present in the study area and indicate that the area has positive prospects for petroleum exploration.

关键词： Hydrocarbon utilizing bacteria microbial prospecting micro-seepage petroleum exploration

Hepatoprotective evaluation of Anogeissus latifolia:In vitro and in vivo studies

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World Journal of Gastroenterology 2009年第38期15卷 4816-4822页

作者： Hulikere Ananth Pradeep Saleemullah Khan Karamkonda Ravikumar mohammed Fazil Ahmed Meesala Srinivasa Rao Mandava Kiranmai Dachani Sudhershan Reddy Shaik rasheed Ahamed mohammed Ibrahim Department of Pharmacology and Biotechnology Nizam Institute of Pharmacy JSS College of Pharmacy OotyTamilnaduIndia and Manipal College of Pharmaceutical Sciences Centre for Liver Research and Diagno-stics Deccan College of Medical Sciences and Allied Hospitals

AIM： To evaluate the hepatoprotective activity of a hydroalcoholic extract of the bark of Anogeissus latifolia; in vitro in primary rat hepatocyte monolayer culture and in vivo in the liver of Wistar rats intoxicated by carbon tetrachloride （CCl4）. METHODS： In the in vitro study, a primary hepatocyte monolayer culture was treated with CCh and extract of Anogeissus latifolia. Hepatoprotective activity was demonstrated in the CCh damaged primary monolayer culture. In the in vivo study, the hepatoprotective activity of a hydroalcoholic extract ofAnogeissus latifolia was analyzed in liver injured CCh-treated rats. Biochemical parameters including serum transaminases [aspartate aminotransferase （AST） and alanine aminotransferase （ALT）] and alkaline phosphatase （ALP） in serum wereanalyzed. The biochemical findings were supplemented with histopathological examination of rat liver sections. RESULTS： In vitro： primary hepatocyte monolayer cultures were treated with CCh and extract of Anogeissus latifolia. A protective activity could be demonstrated in the CCh damaged primary monolayer cultUre. In vivo： Hydroalcoholic extract of Anogeissus latifolia （300 mg/kg） was found to have protective activity in rats with CCh-induced liver damage as judged from serum marker enzyme activity. CONCLUSION： The above findings lead to the conclusion that the hydroalcoholic extract of Anogeissus latifol/a is hepatoprotective. Hence, we suggest that the inclusion of this plant in the management of liver disorders is justified.

关键词： Anogeissus latifolial Hepatoprotective Carbon tetrachloride

Biochemical association between the prevalence of genetic polymorphism and myocardial infarction

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BIOCELL 2023年第3期47卷 473-484页

作者： MOMINA SHAHID KANWAL REHMAN MUHAMMAD SAJID HAMID AKASH SHALEEM SUHAIL SUMBAL rasheed MUHAMMAD IMRAN mohammed AASSIRI Department of Pharmaceutical Chemistry Government College UniversityFaisalabad38000Pakistan Department of Pharmacy The Women UniversityMultan60000Pakistan Research Center for Advanced Materials Science(RCAMS) King Khalid UniversityAbha62413Saudi Arabia Department of Chemistry Faculty of ScienceKing Khalid UniversityAbha62413Saudi Arabia

Genetic polymorphism has a vital role in the pathogenesis and development of myocardial infarction(MI).Single nucleotide polymorphism at any one of the amino acid sequences can result in a diseased state.A single gene can exhibit genetic polymorphism at more than one position giving rise to different *** polymorphism of angiotensinogen(AGT)M235T,AGT T174M,and angiotensin-1-converting enzyme(ACE)I/D,endothelial nitric oxide synthase(eNOS),and methylenetetrahydrofolate reductase(MTHFR)can be a risk factor for ***,it is important to study the prevalence of genetic polymorphisms of these genes among different *** is influenced by genetic polymorphism of various genes,including AGT,ACE,eNOS,MTHFR,***,the association of genetic polymorphism of these genes varies among different populations,but different ethnic groups could show contradictory *** genes have shown a positive association with risks of MI in some populations,whereas the results have not been consistent with every ethnic *** this article,we have summarized the genetic variations in the aforementioned genes and their association with MI.

关键词： Endothelial nitric oxide synthase Methylenetetrahydrofolate reductase AGT M235T AGT T174M

Distributions of pigments in reef sediments, contribution of phytoplankton to organic matter budget in coral reef

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Natural Science 2011年第5期3卷 344-350页

作者： mohammed rasheed Tariq Al-Najjar Said Damhoureyeh 不详

The temporal distributions of pigment on bio-genic calcareous and terrigenic reef sediments, chlorophyll a, chlorophyll b, chlorophyll c, fuco- xanthin, and porphine concentrations were mea- sured monthly in two sediment columns (0 - 15 cm) for one year. Pigment concentrations in-creased significantly during winter (Novem-ber-April) in both sediment types particularly in the upper layers of the sediments. Phytoplankton contributions to organic matter were found to be 8 ± 3 and 6 ± 2% in calcareous and terrigenous sediments respectively. The accumulation and the successive degradation of phytoplankton detritus to inorganic nutrients in calcareous sand may partly sustain the productivity of the coral reef communities which live in nutrient-poor environments.

关键词： Pigment Calcareous Sediments Terrigenous Sediments

Immunomodulatory and Antioxidativepotentials of adipose-derived Mesenchymalstem cells isolated from breast versusabdominal tissue: a comparative study

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Cell Regeneration 2020年第1期9卷 190-203页

作者： Nourhan Abu-Shahba Marwa Mahmoud Mazen Abdel-rasheed Yasmine Darwish Ahmad AbdelKhaliq Eman mohammed Mahmoud ElHefnawi Osama Azmy Stem Cell Research Group Centre of Excellence for Medical ResearchNational Research CentreCairoEgypt Medical Molecular Genetics Department Human Genetics and Genome Research DivisionNational Research CentreCairo 12622Egypt Department of Reproductive Health Research Medical Research Division.National Research CentreCairoEgypt Plastic and Reconstructive Surgery Unit General Surgery DepartmentKasr Al Ainy School of MedicineCairo UniversityCairoEgypt Biomedical Informatics and Chemoinformatics Group Centre of Excellence for Medical ResearchInformatics and Systems DepartmentNational Research CentreCairoEgypt

Background: Adipose-derived stem cells (ASCs) are considered ideal candidates for both research and cellular therapydue to ease of access, large yield, feasibility, and efficacy in preclinical and clinical studies. Unlike the subcutaneousabdominal fat depot, breast ASCs features are still not well recognized, limiting their possible therapeutic use. ASCswere found to exert immunomodulatory and antioxidative activities for maintaining homeostasis and functionality ofdiseased/damaged tissues. This study aims to investigate the immunomodulatory and antioxidative potentials of breastversus abdominal isolated ASCs to find out which anatomical site provides ASCs with better immunoregulatory andoxidative stress resistance ***: ASCs were isolated from abdominal and breast tissues. Gene expression analysis was conducted for a panelof immunomodulatory and antioxidative genes, as well as adipokines and proliferation genes. Flow cytometric analysisof a group of immunomodulatory surface proteins was also performed. Finally, the significantly expressed genes haveundergone protein-protein interaction and functional enrichment in silico ***: Our results revealed similar morphological and phenotypic characteristics for both breast and abdominalASCs. However, a significant elevation in the expression of two potent immunosuppressive genes, IL-10 and IDO aswell as the expression of the multifaceted immunomodulatory adipokine, visfatin, was detected in breast versusabdominal ASCs. Moreover, a significant overexpression of the antioxidative genes, GPX1, SIRT5, and STAT3 and theproliferation marker, Ki67, was also observed in breast ASCs relative to abdominal ones. In silico analysis showed thatboth of the differentially upregulated immunomodulatory and antioxidative mediators integratively involved inmultiple biological processes and pathways indicating their functional ***: Breast ASCs possess superior immunomodulatory and antioxidative c

关键词： Adipose-derived stem cells(ASCs) Breast adipose tissue Abdominal adipose tissue Immunomodulatory potential Antioxidative potential

Effects of Process and Storage Temperature on Browning Index, Furosine, and HMF of Aseptic Cold Break Tomato Paste during Storage Time

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Journal of Food Science and Engineering 2016年第5期6卷 237-244页

作者： Hye Won Yeom Joseph Conte rasheed mohammed Steve DeMuri Campbell Soup Company 1 Campbell Place Camden New Jersey 08103 USA

Effects of process and storage temperature on concentration of Maillard compounds in aseptic cold break tomato paste were evaluated during 12 months of storage time. Cold break tomato paste was processed at 104 ℃ or 112℃, aseptically filled into 5 liter bags, and stored at 21 ℃ and 33 ℃. Level of Browning Index, Furosine, and HMF was monitored in the aseptic cold break tomato paste during the storage time. Browning Index, Furosine, and HMF significantly increased in the aseptic cold break tomato paste during storage at 33℃, and there was linear correlation between Browning Index and Furosine （or HMF）. At the lower storage temperature of 21℃, no significant increases were observed. Effects of the process temperature on Browning Index, Furosine, and HMF of aseptic cold break tomato paste were less significant than the storage temperature.

关键词： Aseptic cold break tomato paste process temperature storage temperature Browning Index Furosine HMF.

Influence of Magnetic Treatment on Salts Leaching： From Soils of Different Salinity Levels

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Journal of Agricultural Science and Technology(B) 2014年第6期4卷 434-439页

作者： Khalida Abdul-Karim Hassan Farhad Ali Hashim Sarwar mohammed rasheed Ahmed Department of Soil and Water Faculty of Agriculture and Forestry Duhok University Duhok 42001 Kurdistan Region Iraq

Leaching behavior of individual ions from three saline soils （S1 = 85.0 dS/m, S2 = 53.92 dS/m and S3 = 38.14 dS/m） under magnetic water （MW） treatment or non-magnetized well water （WW） was investigated in column study. After five pore volumes, soil electrical conductivity （EC） decreased to a range between 2.99-3.29 dS/m under the two water treatments. The rate of soluble cations leached was Na＋〉 Ca2＋〉 Mg2＋〉 K＋ and CI- 〉 SO42- 〉 HCO3- for anions under the two water treatments. The greater amount of salt leached was during the first two pore volumes and was higher under MW treatment. Among soils, the higher the initial EC, the greater the amount of the salt leached. Measurements of the exchangeable Na~ performed at the end of leaching experiment showed a high decrease in soil exchangeable Na＋ compared to the initial values of sodium adsorption ratio （SAR） and exchangeable sodium percentage （ESP） in the soil indicated that there was no prospects of soil sodification after leaching with five pore volumes.

关键词： MW soil salinit leaching