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In Vitro Development and Germination of Immature Embryos of Sweet Oranges Parson Brown and Pineapple(Citrus sinensis)

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Journal of Agricultural Science and Technology(B) 2018年第7期8卷 405-419页

作者： Karim Mahmoudi Najat Handaji mohammed ibriz Najat Arsalane Tarik Aderdour Kawtar Label Hanae Ennaciri Hamid Benyahya Laboratory for the Improvement and Conservation of Plant Genetic Resources National Institute of Agronomic Research(INRA)KenitraBP 257Morocco Laboratory of Genetics and Biometry Faculty of SciencesUniversity Ibn TofailKenitraMorocco

Oranges are classified as the most traded fruit variety *** diversification of the varietal profile of this group is among the objectives of the Citrus breeding ***,the creation of new triploid hybrids via immature embryos rescue,can lead to an increase in gene pool and produce seedless *** addition,flow cytometry was successfully used for analyzing ploidy levels and genetic origin of triploid *** present study was aimed at optimizing immature embryos in vitro germination and plantlet development based on medium chemical composition of the two orange varieties,Pineapple and Parson *** the fruit gets matured,they are harvested and the seeds extracted are classified according to their *** those which are small are cultured in a sterile Murashige and Tucker(MT)basal *** different concentrations of growth regulators are put to the test in order to gain the best medium for seeds development.M1(MT+1 mg/L gibberellic acid(GA3)),M2(MT+1 mg/L kenitin+0.5 mg/L 6-benzylaminopurine(BAP)+0.1 naphthalene acetic acid(NAA)),M3(MT+25 mg/L adenine sulfate(ADS)),M4(MT+0.5 mg/L kenitin+0.5 mg/L BAP+1 mg/L GA3).Percentage of germinated embryos(PGE)is the highest in M3 with 100%and 90%for both Pineapple and Parson Brown varieties,*** addition,the germination interval average(GIA)is shorter in M1 for both varieties,which takes 6 d and 7 d,*** regard to speed seedling growth(mm/week),both varieties undergo significant change in all four ***,the highest rate of acclimatization for the Pineapple and Parson Brown varieties is 80%and 90%in *** general,the smaller the embryos are,the more sensitive they are to culture medium *** is therefore essential to optimize the medium components in order to stimulate their in vitro ***,M1(MT+1 mg/L GA3)remains the best to promote short term good germination and produce a better rate of acclimatization.

关键词： Citrus orange triploids in vitro germination immature embryos flow cytometry genetic

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