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Identification of IL11ra and MELK amplification in gastric cancer by comprehensive genomic profiling of gastric cancer cell lines

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World Journal of Gastroenterology 2016年第43期22卷 9506-9514页

作者： Danielle Queiroz Calcagno Sylvia Santomi takeno Carolina Oliveira Gigek mariana Ferreira Leal Fernanda Wisnieski Elizabeth Suchi Chen taíssa maíra Thomaz Araújo Eleonidas Moura Lima maria Isabel Melaragno Samia Demachki Paulo Pimentel Assumpcao Rommel Rodriguez Burbano marília Cardoso Smith Disciplina de Genética Departamento de Morfologia e GenéticaUniversidade Federal de Sao Paulo Núcleo de Pesquisas em Oncologia Hospital Universitário Joao de Barros Barreto Departamento de Biologia Molecular Universidade Federal da Paraíba Disciplina de Gastroenterologia Cirúrgica Universidade Federal de Sao Paulo Departamento de Ortopedia e Tra.ma.ologia Universidade Federal de Sao Paulo

AIM To identify common copy number alterations on gastric cancer cell *** Four gastric cancer cell lines(ACP02, ACP03, AGP01 and PG100) underwent chromosomal comparative genome hybridization and array comparative genome hybridization. We also confirmed the results by fluorescence in situ hybridization analysis using the bacterial artificial chromosome clone and quantitative real time PCR *** The amplification of 9p13.3 was detected in all cell lines by both methodologies. An increase in the copy number of 9p13.3 was also confirmed by fluorescence in situ hybridization analysis. Moreover, the interleukin 11 receptor alpha(IL11ra) and maternal embryonic leucine zipper kinase(MELK) genes, which are present in the 9p13.3 amplicon, revealed gains of the MELK gene in all the cell lines studied. Additionally, a gain in the copy number of IL11 ra and MELK was observed in 19.1%(13/68) and 55.9%(38/68) of primary gastric adenocarcinoma samples, respectively. CONCLUSION The characterization of a small gain region at 9p13.3 in gastric cancer cell lines and primary gastric adenocarcinoma samples has revealed MELK as a candidate target gene that is possibly related to the development of gastric cancer.

关键词： IL11ra Gastric cancer Genomic profiling MELK 9p13.3

基于网络药理学及实验验证探讨三百棒治疗类风湿性关节炎骨破坏的作用机制

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中国药理学通报 2024年第10期40卷 1979-1990页

作者：江露张宗星李玮怡刘道忠包卓玛聂青云袁林湖北民族大学风湿性疾病发生与干预湖北省重点实验室湖北民族大学医学部湖北省肾脏病临床医学研究中心湖北恩施445000

目的通过网络药理学及体外实验探讨三百棒(Toddalia asiatica,ta)对类风湿性关节炎(rheumatoid arthritis,ra)骨破坏的影响及可能的作用机制。方法使用网络药理学分析ta抗ra骨破坏的有效成分及作用靶点;利用AutoDock和PyMOL软件进行分... 详细信息

目的通过网络药理学及体外实验探讨三百棒(Toddalia asiatica,ta)对类风湿性关节炎(rheumatoid arthritis,ra)骨破坏的影响及可能的作用机制。方法使用网络药理学分析ta抗ra骨破坏的有效成分及作用靶点;利用AutoDock和PyMOL软件进行分子对接。体外培养MC3T3-e1细胞,通过CCK-8检测三百棒醇提物(Toddalia asiatica alcohol extract,taAE)对细胞活力的影响,并筛选出合适的药物浓度及干预时间;用成骨诱导培养基诱导成骨细胞模型,ALP染色、活性检测及茜素红染色检测其成骨分化情况;Western blot检测通路相关蛋白Wnt3a、β-catenin蛋白的表达,并采用通路抑制剂DKK-1进一步验证taAE是否通过Wnt/β-catenin信号通路调控成骨细胞分化。结果共筛选出ta抗ra骨破坏的靶点158个,核心靶点56个。KEGG信号通路富集主要有癌症通路、磷脂酰肌醇3-激酶/蛋白激酶B信号通路、cAMP信号通路。CCK-8检测结果显示:1 g·L^(-1)taAE可显著提高细胞存活率;ALP染色及ALP活性检测结果示:taAE能显著增加成骨诱导培养基诱导的细胞的染色阳性率及ALP活性;Western blot检测结果显示:taAE可增加Wnt3a、β-catenin蛋白的表达;使用DKK-1抑制剂后上述蛋白的表达降低。结论taAE可通过Wnt/β-catenin信号通路调控成骨细胞分化以达到治疗ra骨破坏的作用。

关键词：类风湿性关节炎骨破坏三百棒网络药理学作用机制

Sanitizers Effect in mango Pulp and Peel Antioxidant Compounds

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Food and Nutrition Sciences 2014年第10期5卷 929-935页

作者： Kamila de Almeida Monaco Sergio marques Costa maíra Rodrigues Uliana Giuseppina Pace Pereira Lima Department of Chemistry and Biochemistry Institute of BiosciencesSao Paulo State University (UNESP)BotucatuBrazil

Effects of ozonated water as sanitizer method on mango was studied on total phenolics, flavonoids, carotenoids and vitamin C of pulp or peel. mango cultivar “Palmer” was harvested and subjected to sanitization treatments by immersion in water, chlorinated water (10 minutes sodium hypochlorite 100 mg·L-1) or ozonated water for 10 and 20 minutes. After the sanitization process, the mangoes were stored at 15°C ± 1°C and 85% ± 5% RH for seven days, followed by 4 days of storage at room temperature (simulating the trading period), totaling 11 days after harvested. mangoes pulp sanitized with ozonated water for 20 minutes showed the highest values of ta, total soluble carbohydrates, vitamin C, carotenoids and flavonoid content. These data suggest that the use of ozonated water may contribute to induce increase antioxidants compounds.

关键词： Ozonated Water Vitamin C Polyphenol Carotenoid

Real-world performance analysis of a novel computational method in the precision oncology of pediatric tumors

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World Journal of Pediatrics 2023年第10期19卷 992-1008页

作者： Barbara Vodicska Júlia Déri Dóra Tihanyi Edit Várkondi EnikőKispéter Róbert Dóczi Dóra Lakatos Anna Dirner Mátyás Vidermann Péter Filotás Réka Szalkai-Dénes István Szegedi Katalin Bartyik Krisztina Míta Gábor Réka Simon Péter Hauser György Péter Csongor Kiss Miklós Garami István Peták Oncompass Medicine Hungary Kft Retek Str.34Budapest 1024Hungary Division of Pediatric Hematology-Oncology Department of PediatricsFaculty of MedicineUniversity of DebrecenDebrecenHungary Department of Pediatrics Albert Szent-Györgyi Medical SchoolUniversity of SzegedSzegedHungary Onco-Hematology Department Velkey LászlóPaediatric Health CentreMiskolcHungary Department of Paediatrics Semmelweis UniversityBudapestHungary Onco-Hematology Department Heim Pál Children’s HospitalBudapestHungary Department of Pharmacology and Pharmacotherapy Semmelweis UniversityBudapestHungary Department of Pharmaceutical Sciences University of Illinois at ChicagoChicagoUSA Genomate Health CambridgeMAUSA

Background The utility of routine extensive molecular profiling of pediatric tumors is a matter of debate due to the high number of genetic alterations of unknown significance or low evidence and the lack of standardized and personalized decision support *** drug assignment(DDA)is a novel computational method to prioritize treatment options by aggregating numerous evidence-based associations between multiple drivers,targets,and targeted *** has been validated to improve personalized treatment decisions based on the outcome data of adult patients treated in the SHIVA01 clinical *** aim of this study was to evaluate the utility of DDA in pediatric *** Between 2017 and 2020,103 high-risk pediatric cancer patients(ram,and samples from 100 patients were eligible for further *** or blood samples were analyzed by whole-exome(WES)or targeted panel sequencing and other molecular diagnostic modalities and processed by a software system using the DDA algorithm for therapeutic decision ***,a molecular tumor board(MTB)evaluated the results to provide therapy *** Of the 100 cases with comprehensive molecular diagnostic data,88 yielded WES and 12 panel sequencing *** identified matching off-label targeted treatment options(actionability)in 72/100 cases(72%),while 57/100(57%)showed potential drug *** reached 88%(29/33)by 2020 due to the continuous updates of the evidence *** approved the clinical use of a DDA-top-listed treatment in 56 of 72 actionable cases(78%).The approved therapies had significantly higher aggregated evidence levels(AELs)than dismissed *** of WES results for targeted panels missed important mutations affecting therapy *** DDA is a promising approach to overcome challenges associated with the interpretation of extensive molecular profiling in the routine car

关键词： Computational decision support Pediatric tumors Precision oncology Tumor board

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海流条件下中性缆缆形及张力的理论研究

船舶力学 2016年第8期20卷 983-991页

作者：邢丹马利斌中国船舶科学研究中心江苏无锡214082

文章考虑缆绳经受的法向与切向流体阻力,在自然坐标系下推导了任意海流中缆绳的动力学方程。建立了中性缆在均匀、线性及组合海流作用下缆形与张力的参数表达式。参数表达式给出了对应海流作用下缆形及张力的物理规律,从而为参数迭代求... 详细信息

文章考虑缆绳经受的法向与切向流体阻力,在自然坐标系下推导了任意海流中缆绳的动力学方程。建立了中性缆在均匀、线性及组合海流作用下缆形与张力的参数表达式。参数表达式给出了对应海流作用下缆形及张力的物理规律,从而为参数迭代求解时迅速收敛指明了数学途径。自行编制程序,算例的定量结果进一步验证了其物理规律,也表明在长缆绳下,忽略切向阻力在工程上是不合适的。

关键词：中性缆缆形线性海流参数表达

Role of mi RNAs and their potential to be useful as diagnostic and prognostic biomarkers in gastric cancer

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World Journal of Gastroenterology 2016年第35期22卷 7951-7962页

作者： Kelly Cristina da Silva Oliveira taíssa maíra Thomaz Araújo Camila Inagaki Albuquerque Gabriela Alcantara Barata Carolina Oliveira Gigek mariana Ferreira Leal Fernanda Wisnieski Fernando Augusto Rodrigues Mello Junior André Salim Khayat Paulo Pimentel de Assumpcao Rommel Mário Rodriguez Burbano marília Cardoso Smith Danielle Queiroz Calcagno Núcleo de Pesquisas em Oncologia Universidade Federal do Pará Hospital Universitário Joao de Barros Barreto Disciplina de Genética Departamento de Morfologia e Genética Universidade Federal de Sao Paulo Laboratório de Citogenética Humana Instituto de Ciências Biológicas Universidade Federal do Pará

Alterations in epigenetic control of gene expression play an important role in many diseases, including gastric cancer. many studies have identified a large number of upregulated oncogenic mi RNAs and downregulated tumour-suppressor mi RNAs in this type of cancer. In this review, we provide an overview of the role of mi RNAs, pointing to their potential to be useful as diagnostic and/or prognostic biomarkers in gastric cancer. Moreover, we discuss the influence of polymorphisms and epigenetic modifications on mi RNA activity.

关键词： Gastric cancer Epigenetic Diagnostic biomarkers miRNAs Prognostic biomarkers

祁连山大野口流域青海云杉种群空间格局及其关联性

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植物生态学报 2020年第11期44卷 1172-1183页

作者：拓锋刘贤德刘润红赵维俊敬文茂马剑武秀荣赵晶忠马雪娥甘肃农业大学林学院兰州730070 甘肃省祁连山水源涵养林研究院(甘肃祁连山森林生态系统国家定位观测研究站甘肃祁连山森林生态监测与评估国际科技合作基地甘肃省森林生态与冻土水文水资源重点实验室甘肃省祁连山森林生态系统野外科学观测研究站)甘肃张掖734000 兰州大学生命科学学院兰州730000

研究青海云杉(Piceacrassifolia)种群不同发育阶段的空间分布格局及其关联性,能揭示其种群的发展规律,推断其潜在的生态学过程或影响机制,进而为该种群的配置、种植和森林的经营与管理等提供指导。该研究基于祁连山大野口流域10.2hm2青... 详细信息

研究青海云杉(Piceacrassifolia)种群不同发育阶段的空间分布格局及其关联性,能揭示其种群的发展规律,推断其潜在的生态学过程或影响机制,进而为该种群的配置、种植和森林的经营与管理等提供指导。该研究基于祁连山大野口流域10.2hm2青海云杉动态监测样地调查资料,分析了青海云杉群落的物种组成和径级结构,并采用点格局方法,通过单变量成对相关函数和双变量成对相关函数对不同年龄阶段青海云杉种群的空间分布格局及其空间关联性进行分析。结果表明:(1)青海云杉种群的径级结构呈倒"J"型分布,属增长型种群,种群自然更新状况良好。(2)青海云杉种群4个径级的个体在小尺度上呈聚集分布,但随着空间尺度的增大,聚集强度逐渐减弱,趋向随机分布;较小径级的个体常呈聚集分布,而随着径级增大,也趋向于随机分布。(3)青海云杉大树个体在小尺度(<4 m)范围内与其他径级个体都呈负关联,随着尺度的增加,大树与幼树表现出正关联且关联性随尺度的增加而减弱,而与小树、中树则表现为无关联或者微弱的负关联;径级相近的个体空间关联性为正关联或无关联,随着个体径级间差距增大,空间关联性转变为无关联或负关联。研究表明,祁连山大野口流域青海云杉种群的分布格局及其关联性随空间尺度和发育阶段而变化,这是由种群自身的生物生态学特性、种内种间关系以及环境条件共同作用的结果。

关键词：祁连山青海云杉种群成对相关函数径级结构空间分布格局空间关联

服务经济社会转型,优化职业教育发展的策略思考

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天津中德应用技术大学学报 2017年第3期 14-19页

作者：马树超上海市教育科学研究院上海200032

中国高铁、通信技术、网络金融、电子商务、水利发电、航空航天、道路桥梁等产业或技术发展正在走向世界前沿,职业教育服务经济社会转型发展具有广阔的空间。我国职业院校布局已经为经济转型发展奠定了基础,国家示范高职院校建设项目提... 详细信息

中国高铁、通信技术、网络金融、电子商务、水利发电、航空航天、道路桥梁等产业或技术发展正在走向世界前沿,职业教育服务经济社会转型发展具有广阔的空间。我国职业院校布局已经为经济转型发展奠定了基础,国家示范高职院校建设项目提供了市场经济体制下发挥政府引导作用的成功范例。面对职业教育发展的新环境新挑战,要优化职业教育发展,坚持面向市场、服务发展、就业导向,准确把握深化产教融合的内涵,全面提高技术技能人才培养质量,做强做优全日制学历职业教育,创新成人职业教育的注册生制度,做大职业培训,以高水平职业院校和高水平专业内涵建设为抓手,提高职业教育服务经济社会转型升级的能力,增强职业院校自信,让中国特色职业教育尤其是高等职业教育成为面向世界舞台奉献中国智慧的职业教育发展新模式。

关键词：职业教育服务经济社会转型发展策略

DNGTz二聚体分子间相互作用的密度泛函理论计算（英文）

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火炸药学报 2017年第5期40卷 30-38页

作者：胡银宁艳利康莹宋纪蓉马海霞西安近代化学研究所陕西西安710065 西北大学化工学院陕西西安710069 故宫博物院文保科技部北京100009

在DFT-B3LYP/6-31G*水平下,求得3,6-二硝基胍基-1,2,4,5-四嗪(DNGTz)二聚体势能面上9种优化几何构型和电子结构。用基组叠加误差(BSSE)和零点能(ZPE)校正,计算了分子间相互作用能,二聚体分子间最大相互作用能为-62.24kJ/mol。由自然键轨... 详细信息

在DFT-B3LYP/6-31G*水平下,求得3,6-二硝基胍基-1,2,4,5-四嗪(DNGTz)二聚体势能面上9种优化几何构型和电子结构。用基组叠加误差(BSSE)和零点能(ZPE)校正,计算了分子间相互作用能,二聚体分子间最大相互作用能为-62.24kJ/mol。由自然键轨道(NBO)分析揭示了分子间相互作用的本质。对优化构型进行振动分析,并基于统计热力学求得温度200.0~800.0K从单体形成二聚体的热力学性质变化。结果表明,二聚主要由强氢键所贡献,而结合能不仅取决于氢键。二聚体Ⅰ、Ⅲ、Ⅳ、Ⅴ和Ⅶ的二聚过程在200.0K均能自发进行,表明二聚体Ⅰ、Ⅲ、Ⅳ、Ⅴ和Ⅶ在室温可以稳定存在。

关键词：高氮含能材料 3,6-二硝基胍基-1,2,4,5-四嗪(DNGTz) 分子间相互作用密度泛函理论(DFT) 自然键轨道分析(NBO) 热力学性质