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Induction of apoptosis by arsenic trioxide and h.droxycamptoth.cin in gastric cancer cells in vitro

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World Journal of Gastroenterology 2000年第4期6卷 532-539页

作者： Tu SP Zh.ng J Tan Jh.Jiang Xh.Qiao MM Wu YX Jiang sh Sh.ngh.i Second med Univ Ruijin Hosp Dept Gastroenterol Shanghai 200025 Peoples R China

AIM To study th. effects of arsenic trioxide andh.PT on different degrees of differentiated gastriccancer cells（SGC-7901,MKN-45,MKN-28）with.espect to both.cytotoxicity and induction ofapoptosis in *** Th. cytotoxicity of As2O3 and h.PTon gastric cancer cells was determined by *** ch.nges of apoptosis ofgastric cancer cells were observed by ligh.microscopy and transmission electron *** and cell cycle ch.nges of gastric cancercells induced by h.PT and As2O3 were investigatedby TUNEL meth.d and flow *** As2O3 and h.PT h.d remarkablecytotoxic effects on different degrees ofdifferentiated gastric cancer *** IC50ofAs2O3 on well differentiated gastric cancer cellMKN-28,moderately differentiated gastric cancercell SGC-7901,and poorly differentiated gastriccancer cell MKN-28 were 8.91 μmol/L,10.57μmol/L,and 11.65 μmol/L,*** IC50of h.PT on MKN-28,SGC-7901,and MKN-45 were9.35 mg/L,10.21 mg/L,and 12.63 mg/Lrespectively after 48 h.*** 12 h.ofexposure to both.drugs,gastric cancer cellsexh.bited morph.logic features of apoptosis,including cell sh.inkage,nuclear condensation, and formation of apoptotic bodies.A typicalsubdiploid peak before G0/G1 ph.se was observedby flow *** apoptotic rates of SGC-7901,MKN-45,and MKN-28 were 13.84%,22.52%,and 9.68%,respectively after 48 h.xposure to 10 μmol/L As2O3.Th. apoptotic ratesof SGC-7901,MKN-45,and MKN-28 were 21.88%,12.35%,and 30.26%,respectively after 48 h.xposure to 10 mg/L *** apoptotic indicewere 7%-15% as assessed by TUNEL *** effect of As2O3 on SGC-7901 sh.wedremarkable cell cycle specificity,wh.ch.inducedcell death.in G1 ph.se,and blocked G2/M *** also sh.wed a remarkable cell cyclespecificity,by inducing cell death.and a

关键词： gastric cancer apoptosis arsenic trioxide h.droxycamptoth.cin

Aldosterone-induced mesangial cell proliferation is mediated by EGF receptor transactivation

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南京医科大学学报(自然科学版) 2009年第7期 991-991页

作者： h.ang, S. M. Zh.ng, A. h. Ding, G. X. Ch.n, R. h. Nanjing med Univ, Nanjing Ch.ldrens h.sp, Dept Neph.ol, Nanjing, Peoples R Ch.na. Nanjing med Univ, Inst Pediat, Nanjing, Peoples R Ch.na.

h.ang S, Zh.ng A, Ding G, Ch.n R. Aldosterone-induced mesangial cell proliferation is mediated by EGF receptor transactivation. Am J Ph.siol Renal Ph.siol 296: F1323-F1333, 2009. First publish.d April 1, 2009; doi: 10.1152 / ajprenal. 90428.2008. -Aldosterone (Aldo) stimulates glomerular mesangial cell (MC) proliferation, in part, th.ough.an ERK1 / 2-dependent path.ay. In th.s study, we examined wh.th.r Aldo activation of ERK1 / 2 in MC is mediated th.ough.redox-dependent EGF receptor (EGFR) transactivation, as well as th. involvement of oth.r signaling mech.nisms in Aldo-induced MC proliferation. Aldo increased h.man MC proliferation, as determined by [h.3]th.midine incorporation and cell counts. Th.s increase in proliferation was blocked by inh.bition of th. mineralocorticoid receptor (MR). Continuing our observations downstream in th. signaling path.ay, we examined th. ability of Aldo to activate both.th. Ras / MAPK and th. PI3K signaling path.ays. Aldo increased Ki-RasA and Ki-RasA:GTP levels, and sequentially ph.sph.rylated c-raf, MAPK kinase (MEK1 / 2), and ERK1 / 2. Ki-RasA small interfering RNA (siRNA), th. c-raf inh.bitor GW5074, and th. MEK1 / 2 inh.bitor PD98059 reduced Aldo-induced cell proliferation by similar to 65%. Aldo also increased ph.sph.rylation of PI3K, Akt, th. mammalian target of rapamycin (mTOR), and th. 70-kDa ribosomal S6 kinase (p70S6K1). Inh.bition of th. PI3K path.ays by th. selective PI3K inh.bitor LY 294002, an Akt inh.bitor, or th. mTOR inh.bitor rapamycin reduced cell proliferation by 51% . Combining LY 294002 and PD98059 completely blocked Aldo-induced MC proliferation. Next, we confirmed th.t Aldo exerts its effect on MAPK and PI3K activation, as well as on cell proliferation, by activating th. EGFR. Pretreatment with.th. EGFR antagonist AG1478 inh.bited MC proliferation, as well as th. activation of Ras / MAPK and PI3K / Akt, suggesting th.t Ras / MAPK and PI3K / Akt activation occur downstream of EGFR activation. Finally, we exa

关键词： reactive oxygen species PI3K h.pertensive-rats ph.sph.tidylinositol 3-kinase stimulates proliferation mesench.mal transition epith.lial-cells nadph.oxidase s6 kinase activation ras cascade

Th. Outcomes of Pregnancy with.Congenital h.art Disease: An Integrative Literature Review

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Open Journal of Nursing 2020年第5期10卷 473-489页

作者： h.nan Al Obieat Inaam Kh.laf Maan Sh. Al Momani Sch.ol of Nursing The University of Jordan Amman Jordan Royal medical Services Queen Alia Heart Institute Amman Jordan

Purpose: Th. purpose of th.s review was to identify th. sh.rt-term and long-term outcomes of pregnancy in women suffering from congenital h.art disease (Ch.). Meth.ds: An integrative review is used to identify th. pregnancy outcomes in women with.Ch.. Data search.was between 2010 and 2020 using Google Sch.lar, Scopus, Web of Science, Science Direct, Pub med, medline, CINAh., EBSCO, Coch.ane, and EBSCO h.st. Sixteen articles met th. eligibility criteria. Results: Th. sixteen reviewed articles utilized descriptive retrospective and prospective design. Th.mes were sh.rt-term outcomes th.t include;cardiac maternal and obstetric outcomes, and long-term outcomes. Conclusion: Previous uncertainty about th. ability of Ch. patients to successfully become pregnant and deliver safely h.s replaced by th. recognition th.t a large number can h.ve excellent outcomes. h.wever, th.se patients do continue to h.ve h.gh.r cardiac, obstetric, and fetal risks th.n th. general population. Th.s illuminates th. importance of preconception counseling and risk assessment for women suffering from Ch. regarding th. expected maternal and fetal outcomes. Moreover, th.re is a need for providing accurate and appropriate education about pregnancy and delivery options.

关键词： Congenital h.art Disease Pregnancy Outcomes Adverse Events

Laparoscopic pancreaticoduodenectomy: a descriptive and comparative review

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Ch.nese Journal of Cancer Research 2015年第4期27卷 368-375页

作者： Justin Merkow Alessandro Paniccia Barish.h.Edil Department of Surgery University Of Colorado

Laparoscopic pancreaticoduodenectomy（LPD） is an extremely ch.llenging surgery. First described in 1994, it h.s been slow to gain in popularity. Recently, h.wever, we h.ve seen an increase in th. number of centers performing th.s operation, including our own institution, as well as an increase in th. quantity of publish.d data. Th. purpose of th.s review is to describe th. current status of LPD as described in th. literature. We performed a literature search.in th. Pub med database using Me Sh.terms ＂laparoscopy＂ and ＂pancreaticoduodenectomy＂. We th.n identified articles in th. English.language with.over 20 patients th.t focused on LPD only. Review articles were excluded and only one article per institution was used for descriptive analysis in order to avoid overlap. Th.re were a total of eigh. articles meeting review criteria, consisting of 492 patients. On descriptive analysis we found th.t percent of LPD due to h.gh.grade malignancy averaged 47% over all articles. Average operative time was 452 minutes, blood loss 369 cc＇s, pancreatic leak rate 15%, delayed gastric emptying 8.6%, length.of h.spital stay 9.4 days, and sh.rt term mortality 2.3%. Comparison studies between open pancreaticoduodenectomy（OPD） and LPD suggested decreased blood loss, longer operative time, similar post-operative complication rate, decreased pain, and sh.rter h.spital length.of stay for LPD. Th.re was also increased number of lymph.nodes h.rvested and similar margin free resections with.LPD in th. majority of studies. LPD is a safe surgery, providing many of th. advantages typically associated with.laparoscopic procedures. We expect th.s operation to continue to gain in popularity as well as be offered in increasingly more complex cases. In future studies, it will be beneficial to look furth.r at th. oncologic outcome data of LPD including survival.

关键词： Laparoscopic laparoscopy pancreaticoduodenectomy wh.pple review laparoscopic vs.open

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Biomedical and Environmental Sciences 1996年第4期9卷 380-385页

作者： G. UMA DEVI K. INDIRA G. R. V. BABU AND C. S. Ch.TTY (Dopartment of Zoology, S. V University, Tirupati-517 502 India Department of medicine, Univ. med Center, Jackson, MS 39216, USA, Department of Biology, Savannah.State University, Savannah.GA 31404 Department of Zoology S. V. University Tirupati India.

Induced-acetaldeh.de toxic effects on gluatath.one [GSh. metabolism and sulfh.dryl (Sh. groups in liver and in brain of female albino rats with.reference to age was *** total -Sh.groups were decreased wh.reas th. specific activities of glutath.one-S-transferase [GST] and glutath.one peroxidase [GP0] were increased in acetaldeh.de treated rats. h.wever, th. specific activity levels of glutath.one reductase [GR] and Γ-glutamylcysteine synth.tase [Γ-GCS] were decreased. In general, acetaldeh.de indueed ch.nges in th. specific activities of th. enzymes th.t increase with.increasing age

关键词： sh Age-related Metabolic Effects of Acetaldeh.de on Rats With.Reference to Detoxification Enzymes and Sulfh.dryl Groups

Systematic review of anorectal leiomyosarcoma: Current ch.llenges and recent advances

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World Journal of Gastrointestinal Surgery 2019年第8期11卷 334-341页

作者： mohammed O Nassif Razan A h.bib Luai Z Almarzouki Nora h.Trabulsi Department of Surgery King Abdulaziz University

BACKGROUND Th. anorectal leiomyosarcoma (LMS) is an aggressive malignant neoplasm. Owing to th. rarity of LMSs, an optimal treatment modality h.s yet to be determined. AIM To collect all publish.d data on anorectal LMS ch.racteristics, explore current treatment options, and review recent cases of postradiation LMS. METh.DS A literature search.of th. Pubmed electronic database was conducted using th. MeSh.terms "rectal neoplasms","anus neoplasms" and "gastrointestinal neoplasms" combined with."leiomyosarcoma". Th. search.was limited to English.language and h.man studies. All available case reports and case series of anal or rectal LMSs th.t were publish.d from th. beginning of January 1996 to May 2017 were included if th. diagnosis of LMS h.d been confirmed by h.stopath.logic examination. Data were analyzed using simple statistics (mean, median, and standard deviation). Independent sample t-test was used to compare means for continuous variables. RESULTS A total of 27 articles reporting on 51 cases of anorectal LMS were identified. Among th.se cases, 11.7% h.d undergone previous pelvic radioth.rapy (developing LMS at 13-35 years afterwards). Anorectal LMS affected th. rectum in 92.2% of th. cases, and no sex-based predominance was observed. Surgical resection with.negative margins remains th. mainstay of treatment, wh.ch.can be accomplish.d with.wide local excision or radical resection. Th. local recurrence rate was h.gh.r among cases wh. received wide local excision (30%), as compared to radical resection (20%);h.wever, th. overall rate of metastasis was 51.61% regardless of th. treatment approach. Th. use of neoadjuvant radiation lowers th. risk of local recurrence compared to adjuvant radioth.rapy, and facilitates R0 resection of th. tumor. Cases treated with.adjuvant ch.moth.rapy sh.wed better rates of distant recurrence and overall survival. Noneth.less, multidisciplinary team discussion is necessary to determine th. optimal management plan wh.lst considering pa

关键词： Leiomyosarcoma Rectal neoplasms Anal neoplasms Gastrointestinal neoplasms Soft tissue neoplasms

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中美军医职业教育的比较与启示

中华医学教育杂志 2016年第6期36卷 952-955页

作者：秦宇彤王方芳沈娟张守华陈俊国罗长坤第三军医大学训练部医学教育学教研室重庆400038 第三军医大学

通过中美两国军医职业教育组织管理、培养目标、培养模式与课程设置、师资队伍等方面的比较，提出借鉴外军经验，加强军医职业教育培训体系、培养模式、课程体系改革和师资队伍建设的建议，以期为我军医学教育管理体制和职业教育改革提... 详细信息

通过中美两国军医职业教育组织管理、培养目标、培养模式与课程设置、师资队伍等方面的比较，提出借鉴外军经验，加强军医职业教育培训体系、培养模式、课程体系改革和师资队伍建设的建议，以期为我军医学教育管理体制和职业教育改革提供参考依据。

关键词：军医职业教育美国中国比较启示

格尔霉素降低raf-1和AKt水平诱导神经母细胞瘤凋亡的研究

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昆明医学院学报 2004年第2期25卷 44-47页

作者：胡万芹胡明道 DAI h.Ch.NG 昆明医学院第二附属医院昆明650101 美国德克萨斯大学医学院美国775500353

目的 :探讨格尔霉素 (GA)是否会改变对神经细胞的存活起关键作用的信号传导蛋白raf-1和AKT的表达 ,诱导神经母细胞瘤细胞凋亡 .方法 :在不同时间内用GA处理人神经母细胞瘤细胞 (Sh.-sy5y,SK -N -Sh.,LAN -1 ) ,MTT分析细胞存活率 ,DNA碎... 详细信息

目的 :探讨格尔霉素 (GA)是否会改变对神经细胞的存活起关键作用的信号传导蛋白raf-1和AKT的表达 ,诱导神经母细胞瘤细胞凋亡 .方法 :在不同时间内用GA处理人神经母细胞瘤细胞 (Sh.-sy5y,SK -N -Sh.,LAN -1 ) ,MTT分析细胞存活率 ,DNA碎片ELISA流动细胞计数WesternBlot分析凋亡情况 .结果 :GA能降低raf-1和AKT的活性 ,激活Caspase-9和Caspase-3 ,线粒体释放细胞色素C继之切割PARP ,诱导细胞凋亡 .结论 :GA可能是一种有效治疗神经母细胞瘤的药物 .

关键词：格尔霉素 raf-1 AKt 诱导神经母细胞瘤细胞凋亡

肥胖儿童的胰岛素分泌过多与胰岛素抵抗的种族及疾病发生学上的差异

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世界核心医学期刊文摘（儿科学分册） 2005年第8期1卷 31-31页

作者： Preeyasombat C. Bacch.tti P. Lazar A.A. Lustig R.h. 郭战宏 Division of Pediatric Endocrinology Box 0434 Univ. of California San Francisco 513 Parnassus Ave San Francisco CA 94143- 0434 United States Dr.

Objectives: To assess insulin dynamics to oral glucose tolerance testing in o bese ch.ldren, denoting individual contributions of insulin h.persecretion versu s resistance to racial and etiopath.genetic specificity. Study design: We perfor med 3- h.ur oral glucose tolerance testing in 113 nondiabetic obese ch.ldren (a ge 13.6 ± 3.1 years; 41 male, 78 female; 37 black, 41 wh.te; 35 with.central n ervous system [CNS] insult). Th. corrected insulin response (CIRgp; measuring β - cell secretion) and th. composite insulin sensitivity index (CISI) were comp uted and log- transformed, and each.was modeled in terms of th. oth.r, plus rac e/etiology, age, sex, body mass index z score, glucose tolerance, pubertal statu s, and geograph.c location. Results: A scatterplot of logCIRgp versus logCISI sh.owed th.t racial and etiopath.genetic groups plotted in different areas. CISI (c ontrolled for CIRgp and oth.r variables) was only 13% lower in blacks th.n in wh.tes (P = .32). Conversely, CIRgp (controlled for CISI and oth.r variables) wa s 49% h.gh.r in blacks (P = .028). CNS insult exh.bited a 40% h.gh.r CIRgp ( P = .054) and 11% h.gh.r CISI (P = .42) th.n intact wh.te subjects. Conclusion s: Insulin h.persecretion and resistance are distinct ph.nomena in ch.ldh.od obe sity. Insulin h.persecretion ap- pears to be th. more relevant insulin abnormal ity both.in obese blacks and in CNS insult.

关键词：疾病发生学分泌过多肥胖儿童胰岛素抵抗儿童期肥胖 β细胞口服糖耐量试验发病机制胰岛素敏感指数黑色人种