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肝细胞肝癌与病毒性肝炎:病毒抗原表达免疫组织化学研究

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暨南大学学报（自然科学与医学版） 1998年第6期19卷 55-56页

作者：郝连杰同济医科大学

肝细胞性肝癌是临床高度恶性肿瘤，病情进展迅速，治疗方法有限。从全球看在男性恶性肿瘤中占６２％，女性恶性肿瘤中占２６％。其发病率有明显的地理差异：在北欧和美国为每年／１０００００～３／１０００００；南撒哈拉非洲和南... 详细信息

肝细胞性肝癌是临床高度恶性肿瘤，病情进展迅速，治疗方法有限。从全球看在男性恶性肿瘤中占６２％，女性恶性肿瘤中占２６％。其发病率有明显的地理差异：在北欧和美国为每年／１０００００～３／１０００００；南撒哈拉非洲和南亚居民每年达１５０／１０００００。临床流行病学研究指出：肝细胞肝癌的发病率与肝炎病毒肝炎的发生率密切相关，特别是乙型肝炎病毒、丙型肝炎病毒和丁型肝炎病毒感染。肝炎病毒肝炎与肝细胞性肝癌之间存在着病因联系，肝细胞性肝癌的发生还可能是嗜酒、黄曲霉、血色素沉着症、α１－抗胰蛋白酶缺乏症和卟啉症等所致慢性肝病的后果。方法：近年用ＰＡＰ法和单克隆或多克隆抗体在肝细胞性肝癌患者的肝组织进行了乙型、丙型和丁型肝炎病毒抗原表达的研究，并与其它慢性肝病作为对照。结果：（１）乙型肝炎表面抗原和核心抗原在肝脏肿瘤和非肿瘤组织的表达：５０例肝细胞性肝癌病人用过氧化酶－抗过氧化酶技术进行研究，在非肿瘤组织内ＨＢｓＡｇ和ＨＢｃＡｇ的检出率为７６％和３０％；在肿瘤组织内为１４％和６％。这一发现与慢性肝脏疾病中的检出率有很大的相似性；（２）丁型肝炎病毒抗原在ＨＢＶ标志阳性肝细胞性肝癌肝脏组织内的表达：１０４份肝细胞性肝癌患者肝?

关键词：肝细胞肝癌病毒性肝炎抗原表达免疫组织化学

Transcriptional coactivator med1 in the interface of anti-estrogen and anti-HeR2 therapeutic resistance

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Cancer Drug Resistance 2022年第2期5卷 498-510页

作者： Gregory Bick Jasmine Zhang elyse e.Lower Xiaoting Zhang Department of Cancer Biology Vontz Center for Molecular StudiesUniversity of Cincinnati College of MedicineCincinnatiOH 45267USA Department of Internal medicine University of Cincinnati College of MedicineCincinnatiOH 45267USA. University of Cincinnati Cancer Center University of Cincinnati College of MedicineCincinnatiOH 45267USA.

Breast cancer is one of the most common cancer and leading causes of death in women in the United States and *** 90%of breast cancers belong to eR+or HeR2+subtypes and are driven by key breast cancer genes estrogen Receptor and HeR2,*** the advances in anti-estrogen(endocrine)and anti-HeR2 therapies for the treatment of these breast cancer subtypes,unwanted side effects,frequent recurrence and resistance to these treatments remain major clinical *** studies have identified eR coactivator med1 as a key mediator of eR functions and anti-estrogen treatment ***,med1 is also coamplified with HeR2 and activated by the HeR2 signaling cascade,and plays critical roles in HeR2-mediated tumorigenesis and response to anti-HeR2 treatment as ***,med1 represents a novel crosstalk point of the HeR2 and eR pathways and a highly promising new therapeutic target for eR+and HeR2+breast cancer *** this review,we will discuss the recent progress on the role of this key eR/HeR2 downstream effector med1 in breast cancer therapy resistance and our de.elopment of an innovative RNA nanotechnology-based approach to target med1 for potential future breast cancer therapy to overcome treatment resistance.

关键词： med1 transcription cofactor estrogen receptor HeR2 therapy resistance RNA nanotechnology

关于建立我国继续医学教育制度的探讨试点二年来的分析

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继续医学教育 1989年第2期3卷 49-54,99页

作者：屠中元程新洁李晚明陈军浙江医科大学继续医学教育处

本文对我校开展继续医学教育试点二年来的初步结果;开展继续医学教育的迫切性必要性;实施过程中的认识与体会进行了探讨,指出了存在的问题并提出了建议。

关键词：继续医学教育

estimation of the minimum effective dose of tramadol for postoperative analgesia in infants using the continual reassessment method

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Frontiers of medicine 2012年第3期6卷 288-295页

作者： Yue’e Dai Dongxu Lei Zhenghua Huang Yan Yin GAllen Finley Yunxia Zuo Department of Anesthesiology and Translational Neuroscience Center West China HospitalSichuan UniversityChengdu 610041China Department of Anesthesiology Sichuan Academy of Medical Sciences&Sichuan Provincial People’s HospitalChengdu 610041China Department of Anesthesia and Psychology IWK Health CentreDalhousie University5850 University AvenueHalifaxNSB3K 6R8Canada

Tramadol is a potent ***,the analgesia efficacy of tramadol,particularly its minimum effective dose(med),is not *** aim of this study is to find med of tramadol for postoperative analgesia in *** continual reassessment method(CRM)was performed to find *** undergoing surgeries were included in the 3 phases of this *** each phase,24 participants were allocated a different tramadol *** intensity was measured by face,legs,activity,cry,consolability(FLACC)measurement at 3-hour *** was considered ineffective if the FLACC score was higher than 4 in 10 at *** phase 1,seven dose le.els were used within the range 0.1-0.4 mg·kg^(-1)·h^(-1).Phase 1 was insufficient to identify the med,and we increased the dose to 0.4-0.8 mg·kg^(-1)·h^(-1) in phase *** 2 was insufficient to identify the *** phase 3,local anesthetic wound infiltration was introduced,and the tramadol dose le.els tested were the same as in phase *** successful analgesia probability of tramadol 0.4 mg·kg^(-1)·h^(-1) was 82.1%(95%CI,0.742-0.925)in phase *** phase 2,it was 84.7%(95%CI,0.789-0.991)with the dose 0.8 mg·kg^(-1)·h^(-1).Phase 1 and phase 2 were insufficient to identify the *** phase 3,the successful analgesia probability for dose 0.35 mg·kg^(-1)·h^(-1) was 96.7%(95%CI,0.853-0.997).We have demonstrated that tramadol provides insufficient analgesia for surgeries considered to cause moderate-to-severe postoperative pain in infants if used as the sole analgesic,and that local anesthetic wound infiltration enhances the efficacy of tramadol.

关键词： tramadol minimum effective dose postoperative analgesia infants continual reassessment method

内镜下注射聚糖酐/透明质酸共聚物治疗小儿膀胱输尿管返流

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临床外科杂志 2005年第6期13卷 369-370页

作者：余玲 A.el Ghoneimi Y.Aigrain 上海市儿童医院小儿外科巴黎ROBeRT DeBRe医院小儿泌尿外科

目的介绍巴黎ROBeRT DeBRe医院的小儿外科应用在膀胱镜下输尿管下端开口处注射聚糖酐/透明质酸共聚物治疗(DeFLUX)治疗小儿膀胱输尿管返流的经验.方法选取从2001～2003年,ROBeRT DeBRe 医院(巴黎)小儿外科治疗的57个患儿,其中男11例,女4... 详细信息

目的介绍巴黎ROBeRT DeBRe医院的小儿外科应用在膀胱镜下输尿管下端开口处注射聚糖酐/透明质酸共聚物治疗(DeFLUX)治疗小儿膀胱输尿管返流的经验.方法选取从2001～2003年,ROBeRT DeBRe 医院(巴黎)小儿外科治疗的57个患儿,其中男11例,女46例.平均年龄71个月.患者有急性肾盂肾炎(APN)史,有药物治疗史,并有不同程度的膀胱输尿管返流.共80侧返流的输尿管,行膀胱镜下输尿管开口处注射DeFLUX.结果治疗的患儿术后1年随访.未发现明显的并发症.被治愈的输尿管共58侧(72.5%),1年内未有尿路感染的45例(78.9%).Ⅰ度返流治愈率82.5%.Ⅱ度返流治愈率75.9%.Ⅲ度返流治愈率68.7%.Ⅳ度返流治愈率0.结论在膀胱镜下输尿管下端注射DeFLUX来治疗小儿膀胱输尿管返流,从而控制因膀胱输尿管返流引起的尿路感染是有效的,且安全和简单.

关键词：内窥镜术膀胱输尿管返流儿童

Chimeric antigen receptor-engineered T-cell therapy for liver cancer

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Hepatobiliary & Pancreatic Diseases International 2018年第4期17卷 301-309页

作者： Yang Chen Chang-Yong e Zhi-Wen Gong Shui Liu Zhen-Xiao Wang Yong-Sheng Yang Xue-Wen Zhang Department of Hepatobiliary and Pancreas Surgery the Second Hospital of Jilin University Department of Hepatobiliary and Pancreatic Surgery China-Japan Union Hospital of Jilin University

Background: Chimeric antigen receptor-engineered T-cell(CAR-T) therapy is a newly de.eloped immunotherapy used in the treatment of cancers. Because CAR-T therapy has shown great success in treating CD19-positive hematological malignancies, its application has been explored in the treatment of solid tumors, such as liver cancer. In this review, we discuss the immune characteristics of liver cancer, the obstacles encountered during the application of CAR-T therapy, and preclinical and clinical progress in the use of CAR-T therapy in patients with liver *** sources: The data on CAR-T therapy related to liver cancers were collected by searching Pub med and the Web of Science databases prior to December 2017 with the keywords "chimeric antigen receptor","CAR-T", "liver cancer", "hepatocellular carcinoma", and "solid tumor". Additional articles were identified by manual search of references found in the primary articles. The data for clinical trials were collected by searching Clinical ***: The liver has a tolerogenic nature in the intrahepatic milieu and its tumor microenvironment significantly affects tumor progression. The obstacles that reduce the efficacy of CAR-T therapy in solid tumors include a lack of specific tumor antigens, limited trafficking and penetration of CAR-T cells to tumor sites, and an immunosuppressive tumor microenvironment. To overcome these obstacles, several strategies have emerged. In addition, several strategies have been de.eloped to manage the side effects of CAR-T, including enhancing the selectivity of CARs and controlling CAR-T activity. To date, no clinical trials of CAR-T therapy against HCC have been completed. However, preclinical studies in vitro and in vivo have shown potent antitumor efficacy. Glypican-3, mucin-1, epithelial cell adhesion molecule, carcinoembryonic antigen, and other targets are currently being ***: The application of CAR-T therapy for liver cancer is just beginning to be expl

关键词： Liver cancer Chimeric antigen receptor-engineered T-cell therapy Immunotherapy Tumor-associated antigen

Biomechanics of posterior shoulder instability-current knowledge and literature review

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World Journal of Orthopedics 2018年第11期9卷 245-254页

作者： Henrik Constantin B?cker Samuel e Galle Mauro Maniglio Melvin Paul Rosenwasser Department of Orthopedic Surgery New York Presbyterian/Columbia University Medical Center Department of Orthopedics HFR Cantonal Hospital of Fribourg

Posterior instability of the shoulder is a rare condition and represents about 10% of shoulder instability. It has become more frequently recognized in the last year,even though it is more difficult to diagnose than anterior shoulder instability. As this form of shoulder pathology is somewhat rare,biomechanical knowledge is limited. The purpose of our study was to perform an extensive literature search,including Pubmed and medline,and to give an overview of the current knowledge on the biomechanics of posterior shoulder instability. The Pub med/medline databases were utilized,and all articles related to posterior shoulder instability and biomechanics were included to form a comprehensive compilation of current knowledge. A total of 93 articles were deemed relevant according to our inclusion and exclusion criteria. As expected with any newly acknowledged pathology,biomechanical studies on posterior shoulder instability remain limited in the literature. Current biomechanical models are performed in a static manner,which limits their translation for explaining a dynamic pathology. Newer models should incorporate dynamic stabiliza-tion of both the rotator cuff and scapulothoracic joint. There is a current lack of knowledge with regards to the pathomechanism of posterior shoulder instability,with no consensus on appropriate treatment regimens. Further investigation is therefore required at both basic science and clinical le.els.

关键词： Posterior shoulder instability Anatomy Shoulder complex Scapula Humerus Glenohumeral

enhanced intracellular calcium induced by urocortin is involved in degranulation of rat lung mast cells

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南京医科大学学报（自然科学版） 2008年第6期28卷 778-778页

作者： Wu, Y. Q. Hu, J. Zhang, R. J. Zhou, C. H. Xu, Y. H. Guan, X. W. Li, S. S. (Nanjing med Univ, Dept Pharmacol, Nanjing 210029, Peoples R China. Xuzhou med Coll, Dept Pharmacol, Xuzhou, Peoples R China) NanjingmedUniv DeptPharmacolNanjing210029PeoplesRChina.XuzhouMedCollDeptPharmacolXuzhouPeoplesRChina

Corticotropin-releasing factor(CRF), which activates the hypothalamic-pituitary-adrenal axis under stress, also has proinflammatory peripheral effects possibly through mast cells. The purpose of this study was to investigate the effect of urocortin (UCN), a 40-amino-acid CRF family peptide, on degranulation and intracellular calcium of rat lung mast cells. The activation and degranulation of mast cells were observed by Toluidine blue staining and transmission electron microscope. The intracellular calcium was investigated using confocal laser scanning microscopy and flow cytometry. The results indicated that all the three different concentrations of UCN(0.1, 1 and 10 mu M) significantly induced the activation and degranulation of rat lung mast cells in vitro. This effect was markedly blocked by selective CRF receptor 1(CRF-R1) antagonist antalarmin, but not by specific CRF receptor 2(CRF-R2) antagonist antisauvagine-30(anti-Svg-30). The results also showed that UCN caused a rapid peak increase inCa2+(i) at point of 300s after UCN treatment, followed by a decrease to a sustained plateau phase. The peak increase inCa2+(i) induced by UCN was significantly inhibited by antalarmin, but not by anti-Svg-30. This effect of UCN onCa2+(i) in rat lung mast cells was also found by flow cytometry. Regression analysis revealed a positive correlation between mast cells degranulation extent and the maximum value ofCa2+(i)(P en together, our present study suggested that UCN induced the increase of Ca2+(i) and degranulation of rat lung mast cells through CRF-R1. These findings may have implications for the pathophysiology of allergic and inflammatory lung disorders such as asthma, which is closely associated with mast cell activation and degranulation. Copyright (c) 2008 S. Karger AG, Basel.

关键词：钙细胞肺部肥大细胞

Methods and parameters of melting curve analysis for identification of Leishmania species:A scoping review

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Asian Pacific Journal of Tropical medicine 2021年第12期14卷 528-542页

作者： Juliana J G Ferreira Fernanda S Nascimento Glaucia e B Marcon eros A de Almeida Sandra C B Costa Laboratório de Diagnóstico de Doenças Infecciosas por Técnicas de Biologia Molecular Departamento de Clínica MédicaFaculdade de Ciências MédicasUniversidade Estadual de Campinas(UNICAMP)Campinas13083-887SPBrazil Fundação Oswaldo Cruz Mato Grosso do Sul(FIOCRUZ MS) Rua Gabriel Abrão92Jardim das NaçõesCampo Grande79081-746 MSBrazil Grupo de estudos em Doença de Chagas Departamento de Clínica MédicaFaculdade de Ciências MédicasUniversidade Estadual de Campinas(UNICAMP)Campinas13083-887SPBrazil

Leishmaniasis is a set of diseases with a worldwide distribution that affects mainly economically underprivileged populations in de.eloping countries. It has a major impact on public health, with a global cost of billions of dollars per year. The treatment and control of leishmaniasis vary according to the Leishmania species involved, which require reliable methods for species identification. Since most of the currently used methods have limitations, there is a need for assays that allow rapid, precise identification of the offending species. Real-time polymerase chain reactions in conjunction with dissociation curve analysis have been used to detect differences in the DNA composition of selected genes of Leishmania spp. Kinetoplast DNA is the main molecular target used because of its high copy number per parasite, but other targets have also been studied. As part of an effort to establish melting temperature standards for each target gene, we have reviewed the pertinent literature available in public databases, including Pub med, Web of Science, Sci elO and LILACS, using the keywords "Leishmania", "leishmaniasis", "realtime PCR", "melting temperature", and "melting curve", alone or in combination. After applying eligibility criteria, 27 articles were selected for analysis. A considerable variation in the methodologies analyzed was found regarding molecular targets, standardization of the methods, reproducibility and specificity. Because of this, statistical analysis was not performed. In most cases, the methods were able to differentiate the parasite at the subgenus le.el or few species regardless of the target chosen.

关键词： Leishmaniasis Melting temperature Real-time PCR