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宁夏医科大学学报 1995年第4期30卷 316-318页

作者：丁永生张宝林郭玉林谭少青尹莉宁夏医学院附属医院放射科宁夏平罗县人民医院宁夏医学院附属医院肿瘤内科

本文报告３３例经胃左动脉插管灌注化疗药物治疗晚期贲门癌，多数病人的临床症状如吞咽困难、患部疼痛得到缓解。一年生存率４２．４％（１４／３３），半年生存率６９．７％（２３／３３）、本组有８例病人灌注后出现消化道出血的严重... 详细信息

本文报告３３例经胃左动脉插管灌注化疗药物治疗晚期贲门癌，多数病人的临床症状如吞咽困难、患部疼痛得到缓解。一年生存率４２．４％（１４／３３），半年生存率６９．７％（２３／３３）、本组有８例病人灌注后出现消化道出血的严重并发症。

关键词：灌注动脉内药物治疗癌贲门

A Comparison of Various Normalization Methods for LC/MS Metabolomics Data

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Advances in Bioscience and Biotechnology 2018年第8期9卷 339-351页

作者： Jacob e. Wulff Matthew W. Mitchell Metabolon Inc. Research Triangle Park NC USA

In metabolomics data, like other -omics data, normalization is an important part of the data processing. The goal of normalization is to reduce the variation from non-biological sources (such as instrument batch effects), while maintaining the biological variation. Many normalization techniques make adjustments to each sample. One common method is to adjust each sample by its Total Ion Current (TIC), i.e. for each feature in the sample, divide its intensity value by the total for the sample. Because many of the assumptions of these methods are dubious in metabolomics data sets, we compare these methods to two methods that make adjustments separately for each metabolite, rather than for each sample. These two methods are the following: 1) for each metabolite, divide its value by the median le.el in bridge samples (BRDG);2) for each metabolite divide its value by the median across the experimental samples (med). These methods were assessed by comparing the correlation of the normalized values to the values from targeted assays for a subset of metabolites in a large human plasma data set. The BRDG and med normalization techniques greatly outperformed the other methods, which often performed worse than performing no normalization at all.

关键词： Metabolomics Normalization Liquid Chromatography Mass Spectrometry TIC

摄食成分与胃食管反流病的患病风险:一项志愿者的横断面研究

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世界核心医学期刊文摘（胃肠病学分册） 2005年第5期1卷 26-27页

作者： el-Serag H.B. Satia J.A. Rabeneck L. 雒向宁 Houston Vet. Aff. med. Center (152) 2002 Holcombe Blvd Houston TX 77030 United States Dr.

Background: Although diet has been associated with gastro-oesophageal reflux disease (GORD), the role of dietary components (total energy, macro and micronutrients) is unknown. We examined associations of GORD symptoms with intakes of specific dietary components. Methods: We conducted a cross sectional study in a sample of employees (non-patients) at the Houston VAMC. The Gastro esophageal Reflux Questionnaire was used to identify the onset, frequency, and severity of GORD symptoms. Dietary intake (usual frequency of consumption of various foods and portion sizes) over the preceding year was assessed using the Block 98 food frequency questionnaire. Upper endoscopy was offered to all participants and oesophageal erosions recorded according to the LA classification. We compared the dietary intake (macronutrients, micronutrients, food groups) of participants with or without GORD symptoms, or erosive oesophagitis. Stepwise multiple logistic regression analyses were used to examine associations between nutrients and GORD symptoms or oesophageal erosions, adjusting for demographic characteristics, body mass index (BMI), and total energy intake. Results: A total of 371 of 915 respondents (41%) had complete and interpretable answers to both heartburn and regurgitation questions and met validity criteria for the Block 98 FFQ. Mean age was 43 years, 260 (70%) were women, and 103 (28%) reported at least weekly oc currences of heartburn or regurgitation. Of the 164 respondents on whom endoscopies were performed, erosive oesophagitis was detected in 40 (24%). Compared to participants without GORD symptoms, daily intakes of total fat, saturated fat, cholesterol, percentage of energy from dietary fat, and average fat servings were significantly higher in participants with GORD symptoms. In addition, there was a dose-response relationship between GORD and saturated fat and cholesterol. The effect of dietary fat became non-significant when adjusted for BMI. However, high saturated f

关键词：胃食管反流患病风险横断面研究糜烂性食管炎反流症状微量营养素日常饮食消化道内镜检查食物频率问卷糜烂程度

Molecularly specific detection of bacterial lipoteichoic acid for diagnosis of prosthetic joint infection of the bone

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Bone Research 2018年第2期6卷 189-196页

作者： Julie e.Pickett John M.Thompson Agnieszka Sadowska Christine Tkaczyk Bret R.Sellman Andrea Minola Davide Corti Antonio Lanzavecchia Lloyd S.Miller Daniel LJ Thorek Division of Nuclear medicine and Molecular Imaging Department of Radiology and Radiological ScienceJohns Hopkins University School of MedicineGaithersburgMDUSA Department of Orthopaedic Surgery Johns Hopkins University School of MedicineBaltimoreMD 21205USA Department of Infectious Disease MedImmune LLCGaithersburgMDUSA Humabs Bio med SA BellinzonaSwitzerland Institute for Research in Biomedicine Universitàdella Svizzera italianaBellinzonaSwitzerland Department of Dermatology Johns Hopkins University School of MedicineBaltimoreMD 21205USA Division of Infectious Disease Department of MedicineJohns Hopkins University School of MedicineBaltimoreMDUSA Cancer Molecular and Functional Imaging Program Department of OncologySidney Kimmel Comprehensive Cancer CenterJohns Hopkins University School of MedicineBaltimoreMD 21205USA

Discriminating sterile inflammation from infection, especially in cases of aseptic loosening versus an actual prosthetic joint infection, is challenging and has significant treatment implications. Our goal was to evaluate a novel human monoclonal antibody（mAb） probe directed against the Gram-positive bacterial surface molecule lipoteichoic acid（LTA）. Specificity and affinity were assessed in vitro. We then radiolabeled the anti-LTA mAb and evaluated its effectiveness as a diagnostic imaging tool for detecting infection via immuno PeT imaging in an in vivo mouse model of prosthetic joint infection（PJI）. In vitro and ex vivo binding of the anti-LTA mAb to pathogenic bacteria was measured with Octet, elISA, and flow cytometry. The in vivo PJI mouse model was assessed using traditional imaging modalities, including positron emission tomography（PeT） with [^（18）F]FDG and [^（18）F]Na F as well as X-ray computed tomography（CT）, before being evaluated with the zirconium-89-labeled antibody specific for LTA（[^（89）Zr]SAC55）.The anti-LTA mAb exhibited specific binding in vitro to LTA-expressing bacteria. Results from imaging showed that our model could reliably simulate infection at the surgical site by bioluminescent imaging, conventional PeT tracer imaging, and bone morphological changes by CT. One day following injection of both the radiolabeled anti-LTA and isotype control antibodies, the anti-LTA antibody demonstrated significantly greater（P 〈 0.05） uptake at S. aureus-infected prosthesis sites over either the same antibody at sterile prosthesis sites or of control non-specific antibody at infected prosthesis sites. Taken together, the radiolabeled anti-LTA mAb, [^（89）Zr]SAC55, may serve as a valuable diagnostic molecular imaging probe to help distinguish between sterile inflammation and infection in the setting of PJI. Future studies are needed to determine whether these findings will translate to human PJI.

关键词： Zr Molecularly specific detection bacterial lipoteichoic acid diagnosis prosthetic infection bone LTA SAC PeT FDG tracer

Chronic pain, posttraumatic stress disorder, and opioid intake: A systematic review

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World Journal of Clinical Cases 2019年第24期7卷 4254-4269页

作者： Alicia e López-Martínez ángela Reyes-Pérez elena Rocío Serrano-Ibá?ez Rosa esteve Carmen Ramírez-Maestre Facultad de Psicología Instituto de Investigaciones Biomédicas (IBIMA)Universidad de Málaga

BACKGROUND The literature suggests that there is a high degree of co-occurrence between chronic pain and posttraumatic stress disorder(PTSD). An association has been found between PTSD and substance abuse. PTSD is a severe disorder that should be taken into account when opioids are prescribed. It has been found that the prevalence of opioid use disorder(OUD) in chronic pain patients is higher among those with PTSD than those without this *** To perform a systematic review on the association between PTSD, chronic noncancer pain(CNCP), and opioid intake(i.e., prescription, misuse, and abuse).MeTHODS We conducted a systematic review following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. The Patient, Intervention,Comparator, and Outcomes(PICOS) criteria were formulated a priori in the protocol of the systematic review. A search was conducted of the PROSPeRO database. In March 2019, searches were also conducted of 5 other databases:Pub med, medLINe, Psyc INFO, Web of Science, and PILOTS. The Scottish Intercollegiate Guidelines Network checklist for cohort studies was used to assess the selected studies for their methodological quality and risk of bias. each study was evaluated according to its internal validity, participant sampling,confounding variables, and the statistical *** A total of 151 potentially eligible studies were identified of which 17 were retained for analysis. Only 10 met the selection criteria. All the studies were published between 2008 and 2018 and were conducted in the United States. The.eligible studies included a total of 1622785 unique participants. Of these, 196516 had comorbid CNCP and PTSD and were consuming opiates. The participants had a cross-study mean age of 35.2 years. The majority of participants were men(81.6%). The most common chronic pain condition was musculoskeletal pain:back pain(47.14% across studies;range: 16%-60.6%), arthritis and joint pain(31.1%;range: 18%-67.5%), and

关键词： Posttraumatic stress disorder Chronic pain Opioid prescription Opioid misuse Opioid abuse

HPV16 L1蛋白在大肠杆菌中的表达、纯化及免疫原性研究

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微生物学杂志 2001年第4期21卷 15-16页

作者：商庆龙谷鸿喜哈尔滨医科大学微生物学教研室黑龙江哈尔滨150086

用IPTG诱导目的工程菌 pQe31 HPV16L1/M 15 (pReP4) ,对表达产物进行SDS PAGe和Westernblot分析 ;用表达的L1蛋白免疫BALB/C小鼠得到抗血清后 ,利用真核源性的VLP粗提物验证小鼠抗血清的特异性。利用IMAC金属亲和层析柱纯化L1蛋白。SDS ... 详细信息

用IPTG诱导目的工程菌 pQe31 HPV16L1/M 15 (pReP4) ,对表达产物进行SDS PAGe和Westernblot分析 ;用表达的L1蛋白免疫BALB/C小鼠得到抗血清后 ,利用真核源性的VLP粗提物验证小鼠抗血清的特异性。利用IMAC金属亲和层析柱纯化L1蛋白。SDS PAGe结果显示表达产物在约 5 7ku处有蛋白条带 ;Westernblot结果证实此条带可与HPV16L1蛋白的单克隆抗体反应 ;纯化后的L1蛋白也同样保留免疫特异性 ;小鼠抗血清可与HPV16L1VLP(病毒样颗粒 )发生特异性反应 ,证实重组表达的L1蛋白具有免疫原性。本实验表明HPV16L1蛋白在工程菌M15 (pReP4)中高效表达。

关键词：人乳头瘤病毒16型 L1蛋白表达免疫原性 HPH16 纯化

Managementofearlyasymptomaticgastrointestinalstromaltumorsofthe stomach

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World Journal of Gastrointestinal endoscopy 2014年第7期6卷 266-271页

作者： Scherübl H Faiss S Knoe.el WT Wardelmann e Hans Scherübl Klinik für Innere Medizin II Gastroenterologie GI Onkologie und Infektiologie Vivantes Klinikum Am Urban 10967 Berlin GermanySiegbert Faiss III. Med. Abteilung Asklepios Klinik Barmbek 22291 Hamburg GermanyWolfram-Trudo Knoefel Klinik für Allgemein- Viszeral- und Kinderchirurgie Universit?tsklinikum 40225 Düsseldorf GermanyEva Wardelmann Gerhard-Domagk-Institut für Pathologie Universit?tsklinikum Münster 48149 Münster Germany

Gastrointestinal stromal tumors(GIST) are the most common mesenchymal tumors of the digestive tract. Approximately two thirds of clinically manifest tumors occur in the stomach, nearly one third in the small bowel, and the rest in the colorectal region with a few cas-es in the esophagus. GIST originate within the smooth muscle layer in the wall of the tubular gastrointestinal tract and grow mostly toward the serosa, far less often toward the mucosa. In the latter case, ulceration may de.elop and can cause gastrointestinal bleeding as the cardinal symptom. However, most GIST of the stomach are asymptomatic. They are increasingly detected incidentally as small intramural or submucosal tumors during endoscopy and particularly during endoscopic ultra-sound. epidemiological and molecular genetic findings suggest that early asymptomatic GIST of the stomach(elf-limiting tumorigenesis. Thus, early(e found in 20%-30% of the.elderly. The mostlyelderly people with early gastric GIST have an excellent GIST-specific prognosis. Patients with early GIST of the stomach can therefore be managed by endoscopic sur-veillance.

关键词： Mirco-gastrointestinal stromal tumors Gas-trointestinal stromal tumor Gastric Neoplasia Cancer endoscopy endoscopic ultrasound

Paediatric primary care in Germany during the early COVID-19 pandemic:the calm before the storm

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Family medicine and Community Health 2021年第2期9卷 40-47页

作者： Malte Kohns Vasconcelos Katharina Weil Daniela Vesterling-Hörner Mehrsad Klemm Tarik el Scheich Hanna Renk Katharina Remke Hans Martin Bosse Institute for medical Microbiology and Hospital Hygiene Heinrich Heine University DüsseldorfDüsseldorfGermany Department for General Paediatrics Neonatology and Paediatric CardiologyUniversity Hospital DüsseldorfDüsseldorfGermany Paediatric Practice Dr.med.Vesterling-Hörner DüsseldorfGermany Kinderärzte im Karree LeverkusenGermany Kinderärzte am Zoo DüsseldorfGermany Department of Paediatric Cardiology Pulmonology and Intensive Care MedicineUniversity Children’s Hospital TübingenTübingenGermany

Objectives Globally,the COVID-19 pandemic has a major impact on healthcare *** effects in primary care are *** study aimed to explore changes in consultation numbers and patient management during the COVID-19 pandemic,and to identify challenges for patient *** Survey of paediatric primary care practices on consultation numbers and patient management changes,and semistructured interviews to identify challenges for patient *** and interviews were partially linked in an explanatory sequential design to identify patient groups perceived to be at higher risk for worse care during the *** In and around Düsseldorf,a densely populated area in Western *** primary care facilities are spread over an area with approximately 2 million *** Primary care in Germany is provided through practices run by self-employed specialist physicians that are contracted to offer services to patients under public health insurance which is compulsory to the majority of the *** sample contained 44 paediatric primary care practices in the area,the response rate was 50%.Results Numbers of consultations for scheduled de.elopmental examinations remained unchanged compared with the previous year while emergency visits were strongly reduced(mean 87.3 less/week in March-May 2020 compared with 2019,median reduction 55.0%).Children dependent on de.elopmental therapy and with chronic health conditions were identified as patient groups receiving deteriorated *** patient numbers,including of mildly symptomatic children presenting for health certificates,in combination with increased organisational demands and expected staff outages are priority concerns for the *** Primary care paediatricians offered stable service through the early pandemic but expected strained resources for the upcoming *** guidance on which children should present to primary care and who should be tested would h

关键词： winter storm unchanged

effects of cereal fiber on bowel function: A systematic review of intervention trials

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World Journal of Gastroenterology 2015年第29期21卷 8952-8963页

作者： Jan de Vries Paige e Miller Kristin Verbeke De Vries Nutrition Solutions Inc. Center for epidemiology Biostatistics and Computational Biology Exponent Inc. Nutrition and Food Service Edward Hines Jr. VA Hospital Hines Department of Clinical and experimental medicine KU Leuven

AIM: To comprehensively review and quantitatively summarize results from intervention studies that examined the effects of intact cereal dietary fiber on parameters of bowel function. MeTHODS: A systematic literature search was conducted using Pub med and eMBASe. Supplementary literature searches included screening reference lists from relevant studies and reviews. eligible outcomes were stool wet and dry weight, percentage water in stools, stool frequency and consistency, and total transit time. Weighted regression analyses generated mean change(± SD) in these measures per g/d of dietary fiber. ReSULTS: Sixty-five intervention studies among generally healthy populations were identified. A quantitative examination of the effects of non-wheat sources of intact cereal dietary fibers was not possible due to an insufficient number of studies. Weighted regression analyses demonstrated that each extra g/d of wheat fiber increased total stool weight by 3.7 ± 0.09 g/d(P eight by 0.75 ± 0.03 g/d(P equency by 0.004 ± 0.002 times/d(P = 0.0346; 95%CI: 0.0003-0.0078). Transittime decreased by 0.78 ± 0.13 h per additional g/d(P eat fiber among those with an initial transit time greater than 48 ***: Wheat dietary fiber, and predominately wheat bran dietary fiber, improves measures of bowel function.

关键词： Comprehensive review Dietary fiber Wheat bran Cereal Bowel function

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外科病人之营养

中华医学杂志 1947年第1期33卷 36-36页

作者： e. F. Bensley (1946) Canadian med . Ass. J. 54:283 云林