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Effects of exosomes from mesenchymal stem cells on functional recovery of a patient with total radial nerve injury: A pilot study

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World Journal of Stem Cells 2024年第1期16卷 19-32页

作者： ErdinçCivelek Serdar Kabatas Eyüp Can Savrunlu Furkan Diren Necati Kaplan Demet Ofluoğlu Erdal Karaöz Department of Neurosurgery University of Health SciencesGaziosmanpaşa Training and Research HospitalGaziosmanpaşa 34255IstanbulTurkey Department of Neurosurgery University of Health Sciences TurkeyGaziosmanpaşa Training and Research HospitalGaziosmanpaşa 34255IstanbulTurkey Center for Stem Cell&Gene Therapy Research and Practice University of Health Sciences TurkeyGaziosmanpaşa 34255IstanbulTurkey Department of Neurosurgery Nevşehir State HospitalNevşehir 50300Turkey Department of Neurosurgery Istanbul Rumeli UniversityÇorlu Reyap HospitalÇorlu 59860TekirdağTurkey Department of Physical Medicine and Rehabilitation Ofluoğlu KlinikGöztepe 34728IstanbulTurkey Liv Hospital Center for Regenerative Medicine and Stem Cell Research&Manufacturing(LivMedCell)Beşiktaş34340IstanbulTurkey Department of Histology and Embryology Istinye UniversityFaculty of MedicineZeytinburnu 34010IstanbulTurkey Istinye University Center for Stem Cell and Tissue Engineering Research and PracticeBeşiktaş34340IstanbulTurkey

BACKGROUND Peripheral nerve injury can result in significant clinical complications that have uncertain ***,there is a lack of effective pharmacological interventions for nerve damage,despite the existence of several small compounds,Despite the objective of achieving full functional restoration by surgical intervention,the persistent challenge of inadequate functional recovery remains a significant concern in the context of peripheral nerve *** To examine the impact of exosomes on the process of functional recovery following a complete radial nerve *** A male individual,aged 24,who is right-hand dominant and an immigrant,arrived with an injury caused by a knife *** cut is located on the left arm,specifically below the *** neurological examination and electrodiagnostic testing reveal evidence of left radial nerve *** sural autograft was utilized for repair,followed by the application of 1 mL of mesenchymal stem cell-derived exosome,comprising 5 billion *** exosome was split into four equal volumes of 0.25 mL each and delivered microsurgically to both the proximal and distal stumps using the subepineural *** patient was subjected to a period of 180 d during which they had neurological examination and electrodiagnostic *** The duration of the patient’s follow-up period was 180 *** increasing Tinel’s sign and sensory-motor recovery were detected even at the 10th wk following nerve *** the conclusion of the 6-mo post-treatment period,an evaluation was conducted to measure the extent of improvement in motor and sensory functions of the *** assessment was based on the British Medical Research Council scale and the Mackinnon-Dellon *** results indicated that the level of improvement in motor function was classified as M5,denoting an excellent ***,the level of improvement in sensory function was classified as S3+,indicating a good *** is

关键词： Mesenchymal stem cell Exosomes Radial nerve Sural nerve

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Single-cell immune landscape of measurable residual disease in acute myeloid leukemia

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Science China Life Sciences 2024年

作者： Xiaodong mo Weilong zhang Guomei Fu Yingjun Chang Xiaohui zhang Lanping Xu Yu Wang Chenhua Yan Mengzhu Shen Qiuxia Wei Changjian Yan Xiaojun Huang Peking University People's Hospital Peking University Institute of Hematology National Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Department of Hematology Lymphoma Research Center Peking University Third Hospital Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Research Unit of Key Technique for Diagnosis and Treatments of Hematologic Malignancies Chinese Academy of Medical Sciences

Measurable residual disease(MRD) is a powerful prognostic factor of relapse in acute myeloid leukemia(AML). We applied the single-cell RNA sequencing to bone marrow(BM) samples from patients with(n=20) and without(n=12) MRD after allogeneic hematopoietic stem cell transplantation. A comprehensive immune landscape with 184,231 cells was created. Compared with CD8+T cells enriched in the MRDnegative group(MRD-＿CD8), those enriched in the MRD-positive group(MRD+＿CD8) showed lower expression levels of cytotoxicity-related genes. Three monocyte clusters(i.e., MRD+＿M) and three B-cell clusters(i.e., MRD+＿B) were enriched in the MRD-positive group. Conversion from an MRD-positive state to an MRD-negative state was accompanied by an increase in MRD-＿CD8 clusters and vice versa. MRDenriched cell clusters employed the macrophage migration inhibitory factor pathway to regulate MRD-＿CD8 clusters. These findings revealed the characteristics of the immune cell landscape in MRD positivity, which will allow for a better understanding of the immune mechanisms for MRD conversion.

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支架置入联合腔内射频消融和肝动脉灌注化疗治疗晚期胆管癌伴胆道梗阻的多中心回顾性对照研究

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国际医学放射学杂志 2021年第5期 614-615页

作者： Q.Gou L.Wu W.Cui z.mo D.zeng L.Gan 陈小倩

摘要目的评价支架置入联合腔内射频消融(intra-RFA)和肝动脉灌注化疗(HAIC)治疗晚期胆管癌(Ad-BTC)合并胆道梗阻(BO)的疗效和安全性。方法回顾性分析2013

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SIMULATION OF THE MICROSTRUCTURE DISTRIBUTION OF HYDROGEN STORAGE ALLOY AND OPTIMAL DESIGN OF CASTING moULDS

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Acta Metallurgica Sinica(English Letters) 2000年第2期13卷 770-775页

作者： Q.A. Li, H.Q. mo, Y.z. Huo, G. Sang, Y.F. Chen, M.G. Han, Y.G. Chen and M.J. Tu Department of Metal Materials, Sichuan University, Chengdu 610065, China

In this paper,a simulation model for the temperature field in the solidification process and microstructure distribution is presented. Then, the result of simulation for the final microstructure distribution is compared with experiment using 10-Kg ingot of MlNi3.55Al0.3Mn0.4CO0.75 (Ml: Lanthanum-rich Mischmetal) hydrogen stor- age alloy cast in a mould, which agrees with the experiment well. Finally, in order to obtain the expected as-cast microstructure distribution of 15-Kg ingot of MlNi3.55Al0.3Mn0.4Co0.75 alloy, the size of Cu mould is optimized using the model described. The optimized mould is then made and the alloy is cast in it, the expected as-cast microstructure distribution is obtained.

关键词： hydrogen storage alloy microstructure simulation casting

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2000—2020年广西植被生态质量变化及驱动力分析

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广西植物 2024年第5期44卷 907-924页

作者：莫建飞周希源莫伟华陈燕丽广西壮族自治区气象科学研究所南宁530022 广西壮族自治区生态气象和卫星遥感中心南宁530022 桂林市气象局广西桂林541001

为掌握广西植被生态质量的时空变化特征及其驱动机制,该文以植被生态质量指数(ecological quality index,EQI)为评价指标,基于气象、地形、土壤和遥感等多源数据,利用线性趋势分析、相关性分析、地理探测器等方法,分析了2000—2020年广... 详细信息

为掌握广西植被生态质量的时空变化特征及其驱动机制,该文以植被生态质量指数(ecological quality index,EQI)为评价指标,基于气象、地形、土壤和遥感等多源数据,利用线性趋势分析、相关性分析、地理探测器等方法,分析了2000—2020年广西植被生态质量的时空变化特征及其驱动力。结果表明:(1)2000年以来广西植被生态质量指数呈显著增加趋势,区域植被生态明显改善。植被生态质量发展经历了缓慢增长、迅速增长、显著提升等演变阶段。在空间上,广西植被生态质量指数呈现四周高,中间低的特征,高值区逐渐由东部向西部和北部扩展。(2)广西植被生态质量时空演变影响因素差异显著。随海拔高度上升植被生态质量总体变化呈“增加—下降—稳定—上下波动”的趋势。壤土的植被生态质量高,砂土的植被生态质量低。森林和灌草的生态质量较高,农田植被的生态质量较低。植被生态质量与气候驱动因素呈显著正相关关系,受气温和降水共同影响,其中以气温为主要驱动(T)的区域面积最广,降水为主要驱动(P)的区域面积次之,气温降水强驱动([T+P]+)和弱驱动([T+P]-)的区域面积较小。(3)广西植被生态质量变化驱动力受地形、土壤、植被、气候、自然灾害和人类活动的共同影响。自然影响因子解释力排序为植被>地形>土壤>气候,其中植被净初级生产力和植被覆盖度是影响植被生态质量时空分异的最主要因素。自然因子对广西植被生态质量变化的影响存在交互作用,均呈非线性增强及双因子增强关系,其中地形与植被、土壤与植被、气候与植被因子交互作用最明显。自然灾害和人类活动加剧了植被生态质量变化的影响,其中干旱和低温冷害等气象灾害抑制了植被生态质量的改善,而林业生态工程等人为活动促进了植被生态质量的提升。该研究结果为合理制定广西植被生态保护与修复措施提供了科学理论依据和技术支撑。

关键词：植被生态质量时空演变驱动力遥感广西

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In vivo real-time monitoring delayed administration of M2 macrophages to enhance healing of tendon by NIR-II fluorescence imaging

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Nano Research 2024年第5期17卷 4379-4390页

作者： Yuzhou Chen mo Chen Chengxuan Yu Huizhu Li Liman Sai Nguyen T.K.Thanh Yueming Wang Yan Wo Jian zhang Xing Yang Evgenii L.Guryev Andrei V.zvyagin Hao De Min Tang Shiyi Chen Yunxia Li Yuefeng Hao Sijia Feng Jun Chen Sports Medicine Institute of Fudan University Department of Sports MedicineHuashan HospitalFudan UniversityShanghai 200040China Department of Orthopedic Surgery Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Bone and Joint Surgery Department of OrthopedicsRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200001China Department of Physics Shanghai Normal UniversityShanghai 200234China Biophysics Group Department of Physics and AstronomyUniversity College LondonLondon WC1E 6BTUK UCL Healthcare Biomagnetic and Nanomaterials Laboratories London W1S 4BSUK Department of Anatomy and Physiology School of MedicineShanghai Jiao Tong UniversityShanghai 200025China Department of Orthopedics Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhou 215500China Institute of Biology and Biomedicine Lobachevsky State University of Nizhny NovgorodNizhny Novgorod 603950Russia Mathematical Institute University of OxfordOxford OX26GGUK Institute of Natural Sciences School of MathematicsShanghai Jiao Tong UniversityShanghai 200240China

The administration time is a critical but long-neglected point in cell therapy based on macrophages because the incorrect time of macrophage administration could result in diverse outcomes regarding the same macrophage *** this work,the second near-infrared(NIR-II)fluorescence imaging in vivo tracking of M2 macrophages during a pro-healing therapy in the mice model of rotator cuff injury revealed that the behavior of administrated macrophages was influenced by the timing of their *** delayed cell therapy(DCT)group had a longer retention time of injected M2 macrophages in the repairing tissue than that in the immediate cell therapy(ICT)*** Keller-Segel model and histological analysis further demonstrated that DCT altered the chemotaxis of M2 macrophages and improved the healing outcome of the repaired structure in comparison with *** results offer a possible explanation of previous conflicting results on reparative cell therapy and provoke reconsideration of the timing of these therapies.

关键词： macrophage cell therapy NIR-II in vivo imaging delayed therapy tendon healing

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Human-like adrenal features in Chinese tree shrews revealed by multi-omics analysis of adrenal cell populations and steroid synthesis

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zoological Research 2024年第3期45卷 617-632页

作者： Jing-Hang Jiang Yi-Fu Wang Jie zheng Yi-Ming Lei zhong-Yuan Chen Yi Guo Ya-Jie Guo Bing-Qian Guo Yu-Fang Lv Hong-Hong Wang Juan-Juan Xie Yi-Xuan Liu Ting-Wei Jin Bi-Qi Li Xiao-Shu zhu Yong-Hua Jiang zeng-Nan mo Center for Genomic and Personalized Medicine Guangxi Collaborative Innovation Center for Genomic and Personalized MedicineGuangxi Key Laboratory for Genomic and Personalized MedicineGuangxi Medical UniversityNanningGuangxi 530021China Department of Urology First Affiliated Hospital of Guangxi Medical UniversityNanningGuangxi 530021China Reproductive Medicine Center Jingmen People’s HospitalJingChu University of Technology Affiliated Central HospitalJingmenHubei 448000China School of Computer Science and Engineering Yulin Normal UniversityYulinGuangxi 537000China Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed by the Province and Ministry Guangxi Medical UniversityNanningGuangxi 530021China Department of Pathology Second Affiliated Hospital of Guangxi Medical UniversityNanningGuangxi 530007China Department of Obstetrics and Gynecology Second Affiliated Hospital of Guangxi Medical UniversityNanningGuangxi 530007China

The Chinese tree shrew(Tupaia belangeri chinensis)has emerged as a promising model for investigating adrenal steroid synthesis,but it is unclear whether the same cells produce steroid hormones and whether their production is regulated in the same way as in ***,we comprehensively mapped the cell types and pathways of steroid metabolism in the adrenal gland of Chinese tree shrews using single-cell RNA sequencing,spatial transcriptome analysis,mass spectrometry,and *** compared the transcriptomes of various adrenal cell types across tree shrews,humans,macaques,and *** showed that tree shrew adrenal glands expressed many of the same key enzymes for steroid synthesis as humans,including CYP11B2,CYP11B1,CYB5A,and *** analysis confirmed the production of aldosterone,cortisol,and dehydroepiandrosterone but not dehydroepiandrosterone sulfate in the tree shrew adrenal ***,genes in adrenal cell types in tree shrews were correlated with genetic risk factors for polycystic ovary syndrome,primary aldosteronism,hypertension,and related disorders in humans based on genome-wide association ***,this study suggests that the adrenal glands of Chinese tree shrews may consist of closely related cell populations with functional similarity to those of the human adrenal *** comprehensive results(publicly available at http://***:16245/scAGMap/)should facilitate the advancement of this animal model for the investigation of adrenal gland disorders.

关键词： Tree shrew Adrenal gland Dehydroepiandrosterone Genome-wide association studies Disease model

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Delayed gastric emptying is related to cardiovascular autonomic neuropathy in Chinese patients with type 2 diabetes

Delayed gastric emptying is related to cardiovascular autono...

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中华医学会第十次全国内分泌学学术会议

作者： R.W Bian,Q.L Lou,L.B Gu,X.J Ouyang,Y.z mo Diabetes Care and Research Center,Jiangsu Province Institute of Geriatrics,Nanjing,China

Background:and study aims:Delayed gastric emptying (DGE)is the commonest gastrointestinal(GI)complication in type 2 *** aimed to evaluate the association between DGE and cardiovascular autonomic neuropathy(CAN)in type...

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基于重叠网格的潜艇自航模操纵运动数值模拟研究

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船舶力学 2024年第4期28卷 485-500页

作者：陈默张楠陈纪军黄苗苗曾柯中国船舶科学研究中心江苏无锡214082

针对潜艇机动状态非定常运动数值模拟方法提升的实际需求,本文采用基于重叠网格的方法对潜艇六自由度耦合运动及各操纵面的独立运动直接数值建模,并利用滑移网格技术直接模拟艇后螺旋桨的转动,进行潜艇水下自航模的数值模拟方法研究。... 详细信息

针对潜艇机动状态非定常运动数值模拟方法提升的实际需求,本文采用基于重叠网格的方法对潜艇六自由度耦合运动及各操纵面的独立运动直接数值建模,并利用滑移网格技术直接模拟艇后螺旋桨的转动,进行潜艇水下自航模的数值模拟方法研究。通过开展潜艇自航模水下直航、水下回转、水平面z形操舵、紧急倒车等典型工况的数值模拟,重点解决潜艇操纵状态下艇/桨/舵水动力耦合建模、自动舵的PD(proportional-derivative)数值控制实现等问题,建立基于重叠网格的潜艇自航模数值模拟技术途径。同时对潜艇操纵状态下艇体周围流动现象以及运动学与动力学参数时历变化过程进行分析,通过将典型运动参数数值模拟结果与水池物理模型试验结果进行对比,验证其工程预报实用性。研究结果可为潜艇操纵性和适航性预报与评估以及潜艇自航模模型试验技术完善提供技术支撑。

关键词：潜艇自航模重叠网格数值模拟

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tRNA衍生物tRF-5026a在乳腺癌患者血清中的水平及其对乳腺癌细胞增殖、侵袭和迁移影响的实验研究

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肿瘤研究与临床 2024年第3期36卷 184-190页

作者：李聪王承霞莫冬萍江苏省肿瘤医院、南京医科大学附属肿瘤医院、江苏省肿瘤防治研究所检验科南京210009

目的探讨转运RNA(tRNA)衍生物tRF-5026a在乳腺癌患者血清中的水平及对乳腺癌诊断的价值,并探讨其体外对乳腺癌细胞生物学功能的影响及可能机制。方法回顾性选择2016年1月至2019年2月江苏省肿瘤医院住院的60例女性乳腺癌患者(乳腺癌组)... 详细信息

目的探讨转运RNA(tRNA)衍生物tRF-5026a在乳腺癌患者血清中的水平及对乳腺癌诊断的价值,并探讨其体外对乳腺癌细胞生物学功能的影响及可能机制。方法回顾性选择2016年1月至2019年2月江苏省肿瘤医院住院的60例女性乳腺癌患者(乳腺癌组)及同期20例体检健康女性(健康对照组),采用实时定量聚合酶链反应(RT-qPCR)法检测研究对象血清tRF-5026a的相对表达量;以病理诊断为金标准,绘制依据血清tRF-5026a水平诊断乳腺癌的受试者工作特征(ROC)曲线。采用脂质体法将tRF-5026a模拟物(tRF-5026a组)及阴性对照序列(阴性对照组)瞬时转染至人乳腺癌MCF-7细胞和BT549细胞;采用CCK-8法和5-乙炔基-2-脱氧尿苷(EdU)荧光染色检测各组细胞增殖能力,流式细胞术检测各组细胞凋亡水平,Transwell实验检测各组细胞的侵袭和迁移能力,蛋白质印迹法检测各组细胞中上皮间质转化相关蛋白的表达水平。结果健康对照组和乳腺癌组血清中tRF-5026a相对表达量[M(Q1,Q3)]分别为16.58(6.37,26.31)和3.46(0.32,9.01),差异有统计学意义(z=-4.27,P0.05)。tRF-5026a组MCF-7细胞和BT549细胞的增殖能力以及侵袭和迁移能力均低于相对应的阴性对照组细胞(均P<0.05)。tRF-5026a组MCF-7细胞和BT549细胞神经钙黏素及基质金属蛋白酶(MMP)-9和MMP-3蛋白水平均低于相对应的阴性对照组细胞。结论tRF-5026a在乳腺癌患者血清中水平较低,其可能为乳腺癌诊断的一个指标。体外tRF-5026a能抑制乳腺癌MCF-7细胞和BT549细胞增殖、侵袭和迁移,可能与调控上皮间质转化相关。

关键词：乳腺肿瘤转运RNA衍生物生物学标记细胞增殖细胞运动

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