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Ranking environmental and edaphic attributes driving soil microbial community structure and activity with special attention to spatial and temporal scales

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mlife 2024年第1期3卷 21-41页

作者： vadakattu v.s.R.Gupta James M.Tiedje CsIRO Agriculture&Food UrrbraeSouth AustraliaAustralia Centre for Microbial ecology Michigan State UniversityEast LansingMichiganUSA

The incredibly complex soil microbial communities at small scales make their analysis and identification of reasons for the observed structures *** community structure is mainly a result of the inoculum(dispersal),the selective advantages of those organisms under the habitat-based environmental attributes,and the ability of those colonizers to sustain themselves over *** soil is protective,and its microbial inhabitants have long adapted to varied soil conditions,significant portions of the soil microbial community structure are likely ***,a substantial portion of the community will not correlate to often measured soil *** suggest that the drivers be ranked on the basis of their importance to the fundamental needs of the microbes:(i)those that supply energy,i.e.,organic carbon and electron acceptors;(i)environmental effectors or stressors,i.e.,pH,salt,drought,and toxic chemicals;(ii)macro-organism associations,i.e.,plants and their seasonality,animals and their fecal matter,and soil fauna;and(iv)nutrients,in order,N,P,and probably of lesser importance,other micronutrients,and *** relevance of drivers also varies with spatial and time scales,for example,aggregate to field to regional,and persistent to dynamic populations to transcripts,and with the extent of phylogenetic difference,hence phenotypic differences in organismal *** present a summary matrix to provide guidance on which drivers are important for particular studies,with special emphasis on a wide range of spatial and temporal scales,and illustrate this with genomic and population(rRNA gene)data from selected studies.

关键词： abundance community structure diversity functional microbiome microorganisms

supramolecular Polymerization of Hydrogen-Bonded Trimers in Bulk and Aqueous Medium

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CCs Chemistry 2024年第6期6卷 1458-1465页

作者： Freek v.de Graaf stef A.H.Jansen sandra M.C.schoenmakers Bas.v.n Genabeek Marle e.J.vleugels Bart W.l.van den Bersselaar e.W.Meijer Ghislaine vantomme Institute for Complex Molecular systems Laboratory of Macromolecular and Organic ChemistryEindhoven University of Technology5600 MBEindhoven school of Chemistry and RNA Institute University of New South WalesSydneyNew South Wales 2052

synthetic molecules that form functional noncovalent assemblies in water have received much attention for their potential applications as *** this work,we introduce a novel water-compatible monomer based on the phthalhydrazide moiety that assembles into hydrogen-bonded trimeric *** bulk,the material exhibits a cylindrical nanostructure in the liquid-crystalline phase at elevated temperatures,morphologically distinct from the crystalline lamellar *** water,these molecules effectively form cylinders in a one-dimensional fashion,yielding fibrous *** formation of these supramolecular polymers follows a cooperative mechanism,as evidenced by denaturation *** trimerized pattern represents a new category of aqueous su pramolecular polymers with future prospects for functional complex molecular systems.

关键词： noncovalent chemistry supramolecular polymers hydrogen bonding cooperativity nanostructure

exploring the electromagnetic shielding behavior of lanthanum doped calcium nanoferrites

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Journal of Rare earths 2024年第11期42卷 2128-2136页

作者： e.Ahilandeswari K.sakthipandi R.Rajesh Kanna G.Rajkumar B.Ganesh Babu s.Arunmetha Aslam Hossain P.sakthivel v.Rajendran M.srinidhi Raghavan Department of Physics SRM TRP Engineering College Department of electronics and Communication engineering M. Kumarasamy College of Engineering Department of Physics Easwari Engineering College Department of Mechanical engineering SRM TRP Engineering College Centre for Advanced energy studies Department of Electronics and Communication EngineeringKoneru Lakshmaiah Education Foundation smart Materials Research Institute Southern Federal University Centre for Materials science Department of PhysicsScience and HumanitiesFaculty of EngineeringKarpagam Academy of Higher Education Centre for Nanotechnology Academy of Maritime Education and Training (Deemed to be University) Department of Chemistry and Centre for Nano-Materials and Displays B.M.S. College of Engineering

The present study focuses on the synthesis and characterization of lanthanum(la3+)-doped calcium nanoferrites(CalaxFe2-xO4:x=0.025,0.050,0.075 and 0.100) using the sonochemical *** techniques were employed to analyze the effect of la3+infusion,Raman spectroscopy confirms the presence of active A1g,T2gand egmodes in the CalaxFe2-xO4nanoferrite,indicating the formation of an active ferrite *** introduction of la3+doping results in a significant increase in the band gap energy,rendering the nanoferrites insulating（3.23-3,57 ev）.At higher frequencies,the impedance studies reveal minimal losses and better AC conductivity,pointing to improved dielectric *** higher frequencies,the Q-factor of la-doped calcium nanoferrites shows lower electromagnetic *** M-H curve exhibits ferromagnetic behavior,with la3+-doped calcium nano ferrites displaying a saturation magnetization ranging from 12.72 to 18.10 emu/*** incorporation of la3+also induces enhanced electrical polarization,leading to notable dielectric loss and increased absorption of electromagnetic ***,these CalaxFe2-xO4nanoferrites demonstrate potential as effective microwave absorbers across a wide frequency range,with significant shielding absorption observed at 8.8-9.1 GHz.

关键词： Dielectric Magnetism Microwave absorption Nanoferrites Rare earths structural properties

Paper Analytical Device for the Colourimetric Detection of Alkaline Phosphatase in serum and saliva

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Journal of Analysis and Testing 2024年第3期8卷 385-394页

作者： D.Alageswari A.lakshmiDevi P.e.Resmi P.v.suneesh AarathiPradeep T.G.satheeshBabu Department of Chemistry Amrita School of Physical Sciences Coimbatore Amrita Vishwa Vidyapeetham Amrita Biosensor Research lab Amrita School of Engineering Coimbatore Amrita Vishwa Vidyapeetham Amrita Biomedical engineering Centre Amrita School of Engineering Coimbatore Amrita Vishwa Vidyapeetham

A paper analytical device (PAD) was developed for the colourimetric estimation of alkaline phosphatase (AlP). AlP catalyzes the hydrolysis of disodium phenyl phosphate (DsPP) to phenol, which then undergoes oxidative coupling with4-aminoantipyrine (4-AAP) producing a reddish brown-coloured quinone imine. The colourimetric reaction tested in solution has been translated onto a PAD for the point of care (PoC) testing of AlP. A uniform colour was obtained in 25 min with the introduction of AlP onto the reagent drop cast paper device. The images obtained by scanning the PAD were processed using ImageJ software. The colour intensity obtained by image processing was proportional to the concentration of AlP present in the sample. AlP in serum and saliva samples were tested using the PAD in the range of 0 to 528 U/l and0 to 187 U/l, respectively. The interference studies revealed that the PAD was selective to AlP in the presence of other biomolecules. The PAD exhibited a limit of detection (lOD) of 3.3 U/l and a sensitivity of 48.36 a.u/log(U/l) for serum AlP and lOD of 1.69 U/l and a sensitivity of 28.5 a.u/log (U/l) for salivary AlP. The PAD showed good agreement with the clinical methods for real samples.

关键词： Alkaline phosphatase Colourimetry ImageJ Paper analytical device Point of care testing

exploring one material for dual applications:A red-emitting Mg_(2)GeO_(4):eu^(3+)nanophosphor for future generation energy efficient WleDs and dosimetric applications

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Journal of Rare earths 2024年第10期42卷 1835-1845页

作者： Akshay Arjun G.P.Darshan s.C.sharma H.Nagabhushana D.v.sunitha H.B.Premkumar Department of Physics Faculty of Mathematical and Physical SciencesM.S.Ramaiah University of Applied SciencesBengaluru560054India Honorary Professor of law and Forensic Materials Jain UniversityBengaluru560069India C.N.R.Rao Centre for Advanced Materials Tumkur UniversityTumkur572103India school of Applied sciences(Physics) REVA UniversityBengaluru560064India

The envisaged design and developme nt of individual mate rials to excavate a broader spectrum of desired properties and offer multiple applications are highly *** this in mind,a series of forsterite-structured Mg_(2)GeO_(4):eu^(3+)(1 mol%-9 mol%)nanophosphors was synthesized via a solution combustion method using oxalyl dihydrazide as a *** X-ray diffraction patterns confirm the orthorhombic crystal system with a Puma space *** results clearly show irregularly shaped cluster-like structures with aggregation of the *** diffuse reflectance spectra,the optical energy band gap of the Mg_2GeO_4:eu^(3+)(1 mol%-9 mol%)nanophosphors was estimated and obtained to be~4.12-4.32 *** photoluminescence emission spectra exhibit intense peaks at~579,589,610,661,and 707 nm,which are due to characteristic~5D_(0)→~7F_(0),~5D_(0)→~7F_(1),~5Do→~7F_(2),~5D_(0)→~7F_(3)and~5D_(0)→~7F_(4)transitions of eu^(3+)ions,*** Commission International de l'eclairage color coordinates are gradually tuned from pale red(0.5980,0.4012)to a pure red(0.6385,0.3611)*** glow peaks showcase excellent super-linear response at low doses ofγ-irradiations,indicating that the prepared phosphor can be used in thermoluminescent personal dosimetry which is a biological tissue *** aforementioned results demonstrate that the prepared Mg_2GeO_4:eu^(3+)(1 mol%-9 mol%)nanophosphors are considered an excellent candidate for dual applications,i.e.,red component in future generation white-light-emitting diodes(WleDs)and personal dosimetric applications.

关键词： Nanophosphors Photoluminescence Thermoluminescence Dosimetry Photometric properties Rare earths

supplementation of vitamin e or a botanical extract as antioxidants to improve growth performance and health of growing pigs housed under thermoneutral or heat-stressed conditions

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Journal of Animal science and Biotechnology 2024年第3期15卷 1101-1118页

作者： Ysenia v.silva‑Guillen Consuelo Arellano Jeffrey Wiegert R.Dean Boyd Gabriela e.Martinez eric van Heugten Department of Animal science North Carolina State UniversityRaleighNC 27695USA Department of statistics North Carolina State UniversityRaleighNC 27695USA Department of Animal science Texas A&M UniversityCollege StationTX 77843USA Animal Nutrition Research LLCAlvatonKY 42122USA.

Background Heat stress has s.v.re negative consequences on performance and health of pigs,leading to significant economic *** objective of this study was to investigate the effects of supplemental vitamin e and a botanical extract in feed or drinking water on growth performance,intestinal health,and oxidative and immune status in grow-ing pigs housed under heat stress *** Duplicate experiments were conducted,each using 64 crossbred pigs with an initial body weight of 50.7±3.8 and 43.9±3.6 kg and age of 13-week and 12-week,***(n=128)were housed individually and assigned within weight blocks and sex to a 2×4 factorial arrangement consisting of 2 environments(thermo-neutral(21.2℃)or heat-stressed(30.9℃))and 4 supplementation treatments(control diet;control+100 IU/l of D-α-tocopherol in water;control+200 IU/kg of Dl-α-tocopheryl-acetate in feed;or control+400 mg/kg of a botanical extract in feed).Results Heat stress for 28 d reduced(P≤0.001)final body weight,average daily gain,and average daily feed intake(-7.4 kg,-26.7%,and-25.4%,respectively)but no effects of supplementation were detected(P>0.05).serum vitamin e increased(Pvitamin e supplementation in water and in feed(1.64 vs.3.59 and 1.64 vs.3.24),but not for the botanical extract(1.64 vs.1.67 mg/kg)and was greater when supplemented in water ***(P=0.002).liver vitamin e increased(Pvitamin e supplementations in water(3.9 vs.31.8)and feed(3.9 vs.18.0),but not with the botanical extract(3.9 vs.4.9 mg/kg).serum malondialdehyde was reduced with heat stress on d 2,but increased on d 28(interaction,Ps greater(Psupplementation compared to *** proliferation was reduced(P=0.037)in the jejunum under heat stress,but increased in the ileum when vitamin e was supplemented in feed and water under heat stress(interaction,P=0.04).Tumor necrosis factor-αin jejunum and ileum mucosa decreased by heat stress(Ps re

关键词： Botanical extract Growing pigs Heat stress Immune status Oxidative status Tocopherol Water

Telluric iron assemblages as a source of prebiotic phosphorus on the early earth:Insights from Disko Island,Greenland

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Geoscience Frontiers 2024年第5期15卷 415-429页

作者： Oleg s.v.reshchagin Maya O.Khmelnitskaya larisa v.Kamaeva Natalia s.v.asenko Dmitrii v.Pankin vladimir N.Bocharova sergey N.Britvin saint Petersburg state University University Embankment 7-9St.Petersburg 199034Russia Udmurt Federal Research Center Ural BranchRussian Academy of SciencesIzhevsk 426067Russia Nanomaterial Research Center Kola Science CentreRussian Academy of SciencesApatity 184209Russia

Phosphorus is one of the key elements,which determined the emergence of primordial life on our *** source of prebiotic phosphorus was most likely to be easily soluble compounds containing phosphorus in the negative form of oxidation(e.g.,phosphides).The present paper is the first thorough investigation of phosphide-bearing mineral assemblages confined to telluric(terrestrial)native iron from volcanic rocks of Disko Island,*** speciation in given assemblages.v.ries from the solid solution in native iron(up to 0.3 wt.%P),different phosphides–schreibersite Fe_(3)P,nickelphosphide Ni_(3)P,barringerite Fe_(2)P,and phosphates,including fluorapatite,anhydrous Fe-Na phosphates,phosphoran olivine and pyroxene(up to 1 wt.%P).The diversity of observed phosphorus speciation can be explained by the steep changes of redox conditions during subsurface crystallization of iron-phosphide-bearing *** on the available data on likely redox conditions on the early earth,we hypothesize that reactive prebiotic phosphorus may have originated from shallow crustal rocks.

关键词： Prebiotic phosphorus Native iron Phosphide Reduced mineral assemblages

Design and synthesis of synthetic promoters for consistency of gene expression across growth phases and scale in ***

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synthetic and systems Biotechnology 2024年第2期9卷 330-339页

作者： Kristin v.Presnell Omar Melhem sarah M.Coleman Nicholas J.Morse Hal s.Alper McKetta Department of Chemical engineering The University of Texas at Austin200E Dean Keeton St.Stop C0400AustinTX78712USA Institute for Cellular and Molecular Biology The University of Texas at Austin2500 Speedway AvenueAustinTX78712USA

Metabolic engineering and synthetic biology endeavors benefit from promoters that perform consistently(or robustly)with respect to cellular growth phase(exponential and stationary)and fermentation scale(microtiter plates,tubes,flasks,and bioreactors).However,nearly all endogenous promoters(especially in saccharomyces cerevisiae)do not perform in this *** this work,a hybrid promoter engineering strategy is leveraged to create novel synthetic promoters with robustness across these *** a multi-dimensional RNA-seq dataset,promoters with specific phase dependencies were *** enriched with functional transcription factors were identified using MeMe *** motif-containing fragments could impart activity dependence in the opposing ***,we obtain two new promoters with high and consistent expression across both phases by increasing the exponential phase activity of the starting stationary-phase scaffold by 38 and 23-fold ***,we show that these promoters function consistently across.v.rious laboratory growth scales over time in a microtiter plate and in ***,this work presents and validates a new strategy for engineering promoters in *** with high levels of expression that are robust to cellular growth phase and the scale of the culture.

关键词： endogenous synthetic stationary

An oxygenating colloidal bioink for the engineering of biomimetic tissue constructs

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Bio-Design and Manufacturing 2024年第3期7卷 240-261页

作者： seol-Ha Jeong Jarno Hiemstra Patrick v.Blokzijl Rebeca Damian-Ferrara Danilo Martins dos santos Jéssica H.l.da Fonseca Min-Ho Kang Jihyun Kim Dilara Yilmaz-Aykut Mei l.l.Cham-Pérez Jeroen leijten su Ryon shin Division of engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMA 02139USA leijten lab Department of Developmental BioEngineeringFaculty of Science and TechnologyTechnical Medical CentreUniversity of TwenteDrienerlolaan 57522 NB EnschedeThe Netherlands Nanotechnology National laboratory for Agriculture(lNNA) Embrapa InstrumentaçãoSao CarlosSao Paulo 13560-970Brazil Department of Biomedical-Chemical engineering The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea Department of Biotechnology The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea

ensuring a sufficient oxygen supply is pivotal for the success of bioprinting applications since it fosters tissue integration and natural *** in oxygen concentration among diverse tissues necessitates the precise recreation of tissue-specific oxygen levels in imprinted constructs to support the survival of targeted *** oxygen-releasing biomaterials,such as oxygen-generating microparticles(OMPs),have shown promise for enhancing the oxygen supply of microenvironments in injured tissues,whether this approach is scalable for large tissues and whether tissue-specific bioinks with varying OMP concentrations remain printable remain *** study addresses this critical gap by introducing an innovative class of engineered oxygenated bioinks that combine colloidal-based microgels with *** report that incorporating nanosized calcium peroxide(nCaO_(2))and manganese oxide nanosheets(nMnO_(2))into hydrophobic polymeric microparticles enables precise modulation of oxygen release while controlling hydrogen peroxide ***,the fabrication of oxygenating and cytocompatible colloidal gels is achieved using an aqueous two-phase *** study thoroughly evaluates the fundamental characteristics of the resulting bioink,including its rheological behaviors,printability,shape fidelity,mechanical properties,and oxygen release ***,this study demonstrates the macroscopic scalability and cytocompatibility of printed constructs produced via cell-laden oxygenating colloidal *** showcasing the effectiveness of extrusion-based bioprinting,this study underscores how it can be used to fabricate biomimetic tissues,indicating its potential for new *** findings presented here advance the bioprinting field by achieving scalability with both high cell viability and the possibility of mimicking specifically oxygenated *** work thereby offers a promising avenue for the development of functional tissues with e

关键词： 3D bioprinting Bioink Colloidal gels extrusion printing Oxygen-generating microparticle