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Antibodies elicited by Newcastle disease virus-vectored H7N9 avian influenza vaccine are functional in activating the complement system

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Journal of Integrative Agriculture 2024年第6期23卷 2052-2064页

作者： Zenglei Hu ya Huang Jiao Hu Xiaoquan Wang Shunlin Hu Xiufan Liu Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China Yangzhou UniversityYangzhou 225009China Animal Infectious Disease Laboratory School of Veterinary MedicineYangzhou UniversityYangzhou 225009China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou UniversityYangzhou 225009China

H7N9 subtype avian influenza virus poses a great challenge for poultry *** disease virus(NDV)-vectored H7N9 avian influenza vaccines(NDV_(vec)H7N9)are effective in disease control because they are protective and allow mass *** note,these vaccines elicit undetectable H7N9-specific hemagglutination-inhibition(HI)but high IgG antibodies in ***,the molecular basis and protective mechanism underlying this particular antibody immunity remain ***,immunization with an NDV_(vec)H7N9 induced low anti-H7N9 HI and virus neutralization titers but high levels of hemagglutinin(ha)-binding IgG antibodies in *** residues(S150,G151 and S152)in ha of H7N9 virus were identified as the dominant epitopes recognized by the NDV_(vec)H7N9 immune *** transferred NDV_(vec)H7N9 immune serum conferred complete protection against H7N9 virus infection in *** NDV_(vec)H7N9 immune serum can mediate a potent lysis of ha-expressing and H7N9 virus-infected cells and significantly suppress H7N9 virus *** activities of the serum were significantly impaired after heat-inactivation or treatment with complement inhibitor,suggesting the engagement of the complement ***,mutations in the 150-SGS-152 sites in ha resulted in significant reductions in cell lysis and virus neutralization mediated by the NDV_(vec)H7N9 immune serum,indicating the requirement of antibody-antigen binding for complement ***,antibodies induced by the NDV_(vec)H7N9 can activate antibody-dependent complement-mediated lysis of H7N9 virus-infected cells and complement-mediated neutralization of H7N9 *** findings unveiled a novel role of the complement in protection conferred by the NDV_(vec)H7N9,highlighting a potential benefit of engaging the complement system in H7N9 vaccine design.

关键词： H7N9 subtype avian influenza virus NDV vector vaccine antibody immunity complement protection

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蜜蜂越冬期卵黄原蛋白和保幼激素的表达特性研究

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环境昆虫学报 2024年第4期46卷 941-947页

作者：冀全枝徐凯杜亚丽何金明孙智禹蒋海宾牛庆生刘玉玲苏松坤福建农林大学蜂学与生物医药学院福州350002 吉林省养蜂科学研究所吉林132108

为了探究蜜蜂越冬期体内卵黄原蛋白(Vitellogenin,Vg)和保幼激素(Juvenile hormone,JH)的表达特性。本试验分别采集夏繁期(7月)和不同越冬时期(11月、12月、1月、2月)的珲春黑蜂工蜂,通过酶联免疫吸附试验方法检测Vg、JHⅢ及超氧化物歧... 详细信息

为了探究蜜蜂越冬期体内卵黄原蛋白(Vitellogenin,Vg)和保幼激素(Juvenile hormone,JH)的表达特性。本试验分别采集夏繁期(7月)和不同越冬时期(11月、12月、1月、2月)的珲春黑蜂工蜂,通过酶联免疫吸附试验方法检测Vg、JHⅢ及超氧化物歧化酶(Superoxide Dismutase,SOD)的浓度变化,采用qRT-PCR方法检测Vg、JH代谢相关基因的表达量。结果表明:珲春黑蜂越冬期Vg浓度高于夏繁期,在不同越冬期Vg浓度总体呈现先上升后下降的趋势;Vg基因的变化趋势与Vg浓度相一致,而VgR基因表达量与SOD浓度变化趋势相一致,在越冬期水平高于夏繁期,且在整个越冬期呈现下降的趋势。珲春黑蜂越冬期JHⅢ浓度低于夏繁期,且随着越冬持续进行总体呈现持续上升的变化趋势;MFE基因的变化趋势与JHⅢ浓度相近;JhaMT基因表达量越冬期显著高于夏繁期,在整个越冬期呈现先上升后下降的趋势。本研究结果表明,蜜蜂越冬期体内存在Vg和JHⅢ间负相关的调节模型,其通过Vg、JHⅢ代谢相关基因的表达来提高越冬蜂抗氧化能力和抗寒性,为西方蜜蜂抗寒分子机制的深入研究提供了新的研究思路。

关键词：珲春黑蜂卵黄原蛋白保幼激素越冬期

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2014—2022年手卫生干预对医院感染例次发病率的影响

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中国感染控制杂志 2024年第2期23卷 208-213页

作者：丁佳燕沈瑞红周文琴袁雅赟黄妹杨亚蔡冰超班海群傅小芳上海交通大学医学院附属仁济医院感染管理办公室上海200127

目的观察多模式手卫生干预对手卫生依从性的影响,以及手卫生依从性与医院感染例次发病率之间的关系。方法 2014—2022年,某三甲医院感染控制团队对全院医务人员实施多模式手卫生干预,回顾性分析手卫生监测数据的变化趋势、手卫生依从... 详细信息

目的观察多模式手卫生干预对手卫生依从性的影响,以及手卫生依从性与医院感染例次发病率之间的关系。方法 2014—2022年,某三甲医院感染控制团队对全院医务人员实施多模式手卫生干预,回顾性分析手卫生监测数据的变化趋势、手卫生依从率与医院感染例次发病率的相关性。结果病房手卫生用品消耗量呈稳定上升趋势;手卫生依从率从2014年64.98%提高到2022年85.01%(P<0.001),医院感染例次发病率从1.21%下降至0.83%(P<0.05),手卫生依从率与医院感染例次发病率之间存在负相关(r=-0.369,P=0.027)。各季度不同区域及不同岗位的手卫生依从率均有所提高(P<0.001)。各季度5个时刻手卫生依从率中,无菌操作前与接触患者后的手卫生依从率小幅波动;接触患者周围环境后的手卫生依从率呈上升趋势;接触患者前与接触患者体液后的手卫生依从率在2020年之后有所下降(P<0.001)。结论多模式手卫生干预能提高医务人员的手卫生依从性,提高医务人员手卫生意识有利于降低医院感染例次发病率。

关键词：手卫生依从性医务人员医院感染发病率干预

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铜绿假单胞菌oprI基因DNA疫苗及其重组亚单位疫苗免疫效果的评估

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中国预防兽医学报 2024年第3期46卷 285-291页

作者：祝世纪宫强田佳雨李雅静河南科技大学河南省食品微生物工程技术研究中心河南洛阳471023

为评估铜绿假单胞菌opr I基因DNA疫苗和重组亚单位疫苗对小鼠的免疫效果,本实验将铜绿假单胞菌外膜蛋白编码基因opr I克隆至真核表达载体p CAGGS-ha中构建重组质粒p CAGGS-opr I,经酶切鉴定正确后获得DNA疫苗。同时将opr I基因克隆至原... 详细信息

为评估铜绿假单胞菌opr I基因DNA疫苗和重组亚单位疫苗对小鼠的免疫效果,本实验将铜绿假单胞菌外膜蛋白编码基因opr I克隆至真核表达载体p CAGGS-ha中构建重组质粒p CAGGS-opr I,经酶切鉴定正确后获得DNA疫苗。同时将opr I基因克隆至原核表达载体p ET32a中构建重组质粒p ET32a-oprI,经酶切鉴定正确后转入大肠杆菌,经IPTG诱导重组Opr I蛋白(rOprI)的表达,采用亲和层析法纯化后以SDS-PAGE检测,结果显示正确表达了r Opr I,且获得了其纯化蛋白,利用其制备重组亚单位疫苗。分别以DNA疫苗和重组亚单位疫苗免疫BALB/c小鼠,并以铜绿假单胞菌的灭活疫苗和外膜蛋白疫苗为对照,采用间接ELISA法检测免疫后不同时间小鼠血清中的抗体水平和血清中IFN-γ、IL-2和IL-4的含量;初免42 d后以铜绿假单胞菌(1.25×109 cfu/mL,100μL/只)对各组小鼠进行攻毒试验,通过测定各疫苗对小鼠的保护率,评估疫苗的保护效果。结果显示,各疫苗诱导的免疫小鼠血清抗体水平和细胞因子含量均显著高于阴性对照组(P0.05)。小鼠攻毒试验结果显示,DNA疫苗组、重组亚单位疫苗组、外膜蛋白疫苗组和灭活疫苗组小鼠获得的免疫保护率分别为45%、55%、70%和95%。上述结果首次表明以铜绿假单胞菌opr I基因制备的DNA疫苗和r Opr I重组亚单位疫苗均可诱导小鼠产生体液和细胞免疫应答,并可为小鼠提供对铜绿假单胞菌攻毒的免疫保护效果,但二者的免疫效果还需进一步提高。本研究为铜绿假单胞菌疫苗的研究提供了参考依据。

关键词：铜绿假单胞菌 oprI基因 DNA疫苗重组亚单位疫苗免疫效果

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FGFR2 mutation in a Chinese family with unusual Crouzon syndrome

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International Journal of Ophthalmology(English edition) 2016年第10期9卷 1403-1408页

作者： Zi-Li Li Xue Chen Wen-Juan Zhuang Wei Zhao ya-Ni Liu Fang-Xia Zhang Ruo-Shui ha Jin-Hua Wu Chen Zhao Xun-Lun Sheng Ningxia Eye Hospital People Hospital of Ningxia Hui Autonomous Region (First Affiliated Hospital of Northwest University for Nationalities) Yinchuan 750001 Ningxia Hui Autonomous Region China Department of Ophthalmology the First Affiliated Hospital of Nanjing Medical University State Key Laboratory of Reproductive Medicine Nanjing 210029 Jiangsu ProvinceChina Central Laboratory of Ningxia Medical University Yinchuan 750000 Ningxia Hui Autonomous Region China Department of Radiology People Hospital of Ningxia Hui Autonomous Region Yinchuan 750000 Ningxia Hui Autonomous Region China

AIM：To describe the clinical characteristics with genetic lesions in a Chinese family with Crouzon syndrome. METHODS： All five patients from this family were included and received comprehensive ophthalmic and systemic *** sequencing of the FGFR2 gene was employed for mutation *** structure analysis was applied to analyze the structural changes associated with the substitution. RESULTS： All patients presented typical Crouzon features,including short stature,craniosynostosis,mandibular prognathism,shallow orbits with proptosis,and *** phenotypic diversities were *** optic nerves were exclusively detected in the proband and her *** magnetic resonance imaging implied a cystic lesion in her sellar and third ventricular regions.A missense mutation,FGFR2 ***342 Trp,was found as disease *** substitution would generate conformational changes in the extracellular Ig-III domain of the FGFR-2 protein,thus altering its physical and biological ***： We describe the clinical presentations and genotypic lesions in a Chinese family with Crouzon *** intrafamilial phenotypic varieties in this family suggest that other genetic modifiers may also play a role in the pathogenesis of Crouzon syndrome.

关键词： Crouzon syndrome familial cases phenotypic diversity FGFR2 mutation

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Investigation of phytosociological parameters and physicochemical properties of soil in tropical semi-evergreen forests of Eastern Himalaya

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Journal of Forestry Research 2017年第3期28卷 513-520页

作者： Gaurav mishra.P.K.Das Rinkumoni Borah Antara Dutta Rain Forest Research Institute JorhatAssam 785001India

The study of floral diversity in forest and its development are incomplete without taking consideration of plant-soil interactions. So with this view in mind, the present study was conducted in tropical semi-evergreen forests of the Mokukchung district, Nagaland, in eastern Himalaya. The aim of the study was to investigate the phytosociological parameters Seven sites were randomly in relation to soil properties. selected to study the soil properties at up to one meter in depth and a phytosociological study was carried out in nearby areas via the quadrate method. In the studied sites, the richness of tree species varied from 4 to 15 ha^-1, with Gmelina arborea and Duabanga grandifloras being the common species. The highest total basal area was recorded in 10 mile village （47,998.16 cm^2 ha^-1） followed by Minkong village site （32,704.66 cm^2 ha^-1）. Soil physical and chemical properties-i.e, bulk density （BD）, soil pH, organic carbon （OC）, available nitrogen （N）, available phosphorus （P）, and available potassium （K） were analyzed using standard procedures. Significant differences were observed in the soil properties. The basal area of species showed significant positive correlation in terms of available K （0.754） and OM （0.302） content in soil, and the Shannon-Wiener diversity index （H） is also positively correlated with the available N content （0.402）. The undisturbed nature of the sites played an important role in maintaining the soil fertility and floral diversity of the sites. Moreover, sites with maximum productivity and soil fertility are considered as potential carbon sequestration areas in the region while sites with the low soil fertility need restoration.

关键词： Plant diversity Carbon Nitrogen Phosphorus Potassium Nagaland

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Arsenic,Iron and Organic Matter in Quaternary Aquifer Sediments from Western Hetao Basin,Inner Mongolia

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Journal of Earth Science 2017年第3期28卷 473-483页

作者： Naijing Liu yamin Deng ya Wu Geological Survey China University of GeosciencesWuhan 430074China Ningxia Institute of Geological Survey Yinchuan 750001China School of Environmental Studies China University of GeosciencesWuhan 430074China

To investigate geochemical and mineralogical features of sediments from aquifers containing high levels of dissolved arsenic（As） in western Hetao Basin, Inner Mongolia, sediment samples were collected for XRD（X-ray diffraction）, amorphous Fe/Mn oxides, and humic/fulvic acid analyses, in addition to As determination. In the study area, the total As contents in the sediments were in the range 5.3–28.8 mg/kg; while that associated with humic substances in the range 4–9 mg/kg, accounting for 26%–47% of the total As. The results of XRD analysis suggest that clay and silt contain certain small amounts of iron oxides minerals, such as ferrihydrite, hematite and goethite, whereas have higher As and Fe_2O_3 contents. Up to one third of As in the sediments could be extracted by ammonium oxalate, and high As contents were generally found in organic-rich clay or silty clay samples with high FA/ha ratio. As is strongly associated with humic substances and Fe oxyhydroxides, which may be the major sink and source of As in the aquifer sediments.

关键词： arsenic sediment organic matter Hetao Basin

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香兰素下调cGAS/STING信号通路改善肝内胆汁淤积大鼠肝组织损伤

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中国药理学通报 2024年第9期40卷 1695-1700页

作者：姜宁蒲兰香黄峰王艳裴鑫宋均亚张恩胜青岛大学附属山东省妇幼保健院儿科山东济南250014 青岛大学附属山东省妇幼保健院药剂科山东济南250014 青岛大学附属山东省妇幼保健院武警山东省总队医院特色专业一科山东济南250014

目的探讨香兰素调节环鸟苷酸腺苷酸合成酶(cyclic guanylate adenylate synthetase,cGAS)/干扰素基因刺激因子(stimulator of interferon gene,STING)信号通路对肝内胆汁淤积(intrahepatic cholestasis,IC)大鼠肝组织损伤的影响。方法将SD大鼠随机分为正常组、IC组、香兰素组、cGAS过表达组、香兰素+cGAS过表达组,连续给药7 d。测定大鼠体质量、肝质量和肝质量/体质量比值;酶联免疫吸附法测定肝功能指标(ALT、AST、ALP、LDH),IC指标(TBIL、TBA),肝纤维化指标(ha、LN、PCⅢ);HE、Masson染色分别观察肝脏病理学、纤维沉积变化,并计算胶原容积分数;蛋白印迹法检测肝组织cGAS/STING通路相关蛋白表达。结果香兰素可改善IC大鼠肝脏病理、纤维化,升高体质量,降低肝脏质量、ALT、AST、ALP、LDH、TBIL、TBA、ha、LN、PCⅢ、胶原容积分数、cGAS、STING蛋白(P<0.05);cGAS过表达可逆转香兰素对IC大鼠上述指标的影响(P<0.05)。结论香兰素可能通过下调cGAS/STING信号通路,改善IC大鼠肝功能、IC、肝纤维化和肝组织损伤。

关键词：香兰素环鸟苷酸腺苷酸合成酶干扰素基因刺激因子肝内胆汁淤积肝损伤肝纤维化

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Abundance and Community Composition of Ammonia-Oxidizers in Paddy Soil at Different Nitrogen Fertilizer Rates

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Journal of Integrative Agriculture 2014年第4期13卷 870-880页

作者： SONG ya-na LIN Zhi-min Institute of Biological Technology Fujian Academy of Agricultural Sciences

Ammonia oxidation, the first and rate-limiting step of nitrification, is carried out by both ammonia-oxidizing bacteria （AOB） and ammonia-oxidizing archaea （AOA）. However, the relative importance of AOB and AOA to nitrification in terrestrial ecosystems is not well understood. The aim of this study was to investigate the effect of the nitrogen input amount on abundance and community composition of AOB and AOA in red paddy soil. Soil samples of 10-20 cm （root layer soil） and 0-5 cm （surface soil） depths were taken from a red paddy. Rice in the paddy was fertilized with different rates of N as urea of N1 （75 kg N ha＂ yr-1）, N2 （150 kg N ha~ yrl）, N3 （225 kg N ha1 yrl） and CK （without fertilizers） in 2009, 2010 and 2011. Abundance and community composition of ammonia oxidizers was analyzed by real-time PCR and denaturing gradient gel electrophoresis （DGGE） based on amoA （the unit A of ammonia monooxygenase） gene. Archaeal amoA copies in N3 and N2 were significantly （P〈0.05） higher than those in CK and N1 in root layer soil or in surface soil under tillering and heading stages of rice, while the enhancement in bacterial amoA gene copies with increasing of N fertilizer rates only took on in root layer soil. N availability and soil NO3--N content increased but soil NH4＋-N content didn＇t change with increasing of N fertilizer rates. Otherwise, the copy numbers of archaeal amoA gene were higher （P〈0.05） than those of bacterial amoA gene in root lary soil or in surface soil. Redundancy discriminate analysis based on DGGE bands showed that there were no obvious differs in composition of AOA or AOB communities in the field among different N fertilizer rates. Results of this study suggested that the abundance of ammonia-oxidizers had active response to N fertilizer rates and the response of AOA was more obvious than that of AOB. Similarity in the community composition of AOA or AOB among different N fertilizer rates indicate that the community composition of ammonia-oxidizers w

关键词： ammonia-oxidizing bacteria ammonia-oxidizing archaea nitrogen fertilizer rates paddy soil

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疏肝化纤汤对乙肝肝硬化代偿期患者肝纤维化指标及炎症因子水平的影响

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中国药物经济学 2024年第3期19卷 42-45页

作者：张铁成任亚红乐山市中医医院肝病科四川乐山614000

目的探讨疏肝化纤汤对乙肝肝硬化代偿期患者肝纤维化及炎症因子水平的影响。方法选取2021年1月至2023年1月乐山市中医医院收治的乙肝肝硬化代偿期患者120例作为研究对象,随机分为两组,其中对照组(n=60)采用常规治疗,观察组(n=60)在对... 详细信息

目的探讨疏肝化纤汤对乙肝肝硬化代偿期患者肝纤维化及炎症因子水平的影响。方法选取2021年1月至2023年1月乐山市中医医院收治的乙肝肝硬化代偿期患者120例作为研究对象,随机分为两组,其中对照组(n=60)采用常规治疗,观察组(n=60)在对照组基础上采用疏肝化纤汤治疗。比较两组临床疗效、肝纤维化指标、炎症因子水平及不良反应发生情况。结果观察组治疗有效率为91.67%,高于对照组的80.00%(Pha)、层黏蛋白(LN)、Ⅲ型前胶原肽(PC-Ⅲ)及超敏C反应蛋白(hs-CRP)、白细胞介素-1β(IL-1β)、降钙素原(PCT)水平均低于对照组(P0.05)。结论疏肝化纤汤治疗乙肝肝硬化代偿期能提高临床疗效,改善患者肝纤维化及炎症因子水平,且安全性较高。

关键词：乙肝肝硬化代偿期疏肝化纤汤肝纤维化炎症因子

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建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

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