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Efficient detection of pathogen virus in sand dabs,Paralichthys olivaceus using loop-mediated isothermal amplification(LAMP)

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Acta Oceanologica Sinica 2016年第8期35卷 44-50页

作者： HWANG Jinik park So Yun SUH Sung-Suk park mirye LEE Sukchan LEE Taek-Kyun South Sea Environment Research Department Korea Institute of Ocean Science and TechnologyGeoje 53201Republic of Korea Korea University of Science and Technology Daejeon 34113Republic of Korea Department of Genetic Engineering Sungkyunkwan UniversitySuwon 16419Republic of Korea

Viral hemorrhagic septicemia virus（VHSV） and marine birnavirus（MABV） are the causative pathogens for some of the most explosive epidemics of emerging viral diseases in many Asian countries, leading to huge economic losses in aquaculture. Rapid molecular detection for surveillance or diagnosis has been a critical component in reducing the prevalence of pathogen infection. The loop-mediated isothermal amplification（LAMP） of DNA is currently one of the most commonly used molecular diagnostic tools, as it is simple, quick, and easy to amplify target DNA under isothermal conditions. In the present study, a novel and highly specific LAMP assay for the sensitive and rapid detection of VHSV and MABV infection in fish was developed. Using a set of synthesized primers matching a specific region of the genome, the efficiency and specificity of the LAMP assay were optimized in terms of the reaction temperature and DNA polymerase concentration, as they are the main determinants of the sensitivity and specificity of the LAMP assay. In particular, we demonstrated that our assay could be applied to efficient detection of VHSV and MABV infection in the wild fish, Paralichthys olivaceus. Our results demonstrate the simplicity and convenience of this method for the detection of viral infection in aquatic organisms.

关键词： viral hemorrhagic septicaemia virus(VHSV) marine birnavirus(MABV) polymerase chain reaction loop-mediated isothermal amplification

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Phenol content,antioxidant and tyrosinase inhibitory activity of mangrove plants in Micronesia

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Asian Pacific Journal of Tropical Medicine 2014年第7期7卷 531-535页

作者： Sung-Suk Suh Jinik Hwang mirye park Heung-Sik park Taek-Kyun Lee South Sea Environment Research Department.Korea Institute of Ocean Science and Technology Korea South Pacific Ocean Research Center Korea Institute of Ocean Science and Technology

Objective:To find out and compare the in vitro antioxidant and tyrosinase inhibitory activities of two species of mangrove ***:Mangrove samples were harvested at the shoreline on the island of Weno,Chuuk State in *** phenol content,antioxidant activity(based on DPPH-free radical scavenging)and tyrosinase inhibitory activity in different tissues(leaves,barks and roots)of Rhizophora stylosa(***)and Sonneratia alba(***),collected from the island of ***:Total phenol content ranged from 4.87 to 11.96 mg per g of freeze dried *** highest antioxidant activity was observed in *** bark(85.5%).The highest tyrosinase inhibitory activity was found in *** ***,total phenol content and antioxidant activity were higher in methanol extracts than in aqueous ***:Taken together,the results of this study proved that mangroves can be excellent sources of antioxidant compounds.

关键词： Rhizophora stylosa Sonneratia alba Phebolics Antioxidant activity Tyrosinase inhibitory activity

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Integration of the nuclease protection assay with sandwich hybridization (NPA-SH) for sensitive detection of Heterocapsa triquetra

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Acta Oceanologica Sinica 2018年第5期37卷 107-112页

作者： park mirye park So Yun HWANG Jinik JUNG Seung Won 3LEE Juyun CHANG Man LEE Taek-Kyun South Sea Environment Research Department Korea Institute of Ocean Science and Technology Geoje 53201 Republic of Korea Bioresources Culture Collection Division Nakdonggang National Institute of Biological Resources Sangju 37242 Republic of Korea Research Strategy Team Korea Marine Environment Management Corporation Seou105718 Republic of Korea

Microalgae are photosynthetic microorganisms that function as primary producers in aquatic ecosystems. Some species of microalgae undergo rapid growth and cause harmful blooms in marine ecosystems. Heterocapsa triquetra is one of the most common bloom-forming species in estuarine and coastal waters worldwide. Although this species does not produce toxins, unlike some other Heterocapsa species, the high density of its blooms can cause significant ecological damage. We developed a H. triquetra species-specific nuclease protection assay sandwich hybridization（NPA-SH） probe that targets the large subunit of ribosomal RNA（LSU r RNA）. We tested probe specificity and sensitivity with five other dinoflagellates that also cause red tides. Our assay detected *** at a concentration of 1.5×10^4 cells/m L, more sensitive than required for a red-tide guidance warning by the Korea Ministry of Oceans and Fisheries in 2015（3.0×10^4 cells/m L）. We also used the NPA-SH assay to monitor H. triquetra in the Tongyeong region of the southern sea area of Korea during 2014. This method could detect *** cells within 3 h. Our assay is useful for monitoring H. triquetra under field conditions.

关键词： nuclease protection assay sandwich hybridization Heterocapsa triquetra red tide monitoring

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Fatty acid methyl ester profiles and nutritive values of 20 marine microalgae in Korea

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Asian Pacific Journal of Tropical Medicine 2015年第3期8卷 191-196页

作者： Sung-Suk Suh So Jung Kim Jinik Hwang mirye park Taek-Kyun Lee Eui-Joon Kil Sukchan Lee South Sea Environment Research Department Korea Institute of Ocean Science and Technology Kyeongbuk Institute for Marine Bio-Industry Department of Genetic Engineering Sungkyunkwan University

Objecive:To screen the fatty acid(FA) composition of 20 marine microalgae species,including seven Diophyceae,six Bacillariophyeae four Chlorophyceae,two Haptophyceae and one Raphidophyceae ***:Microalgal cells cultured at the Korea Institute of Ocean Science & Technology were harvested during the late exponential growth phase and the FA composition ***:The FA composition of microalgae was *** example,seven different species of Dinophyceae were composed primarily of C14:0,C16:0.C18:0.C20:4n-6.C20:5n-3 and C22:*** C14:0.C16:0,C16:1.C18:0.C20:5n-3 and C22:6n-3 were abundant FAs in six species of *** addition,four Chlurophyceae,two Haptopkyeeae and one Raphidophyceae species all contained a high degree of C16:1 n-7[(9.2R-34.91)%and(34.48-35.04)%].C14:0[(13.34-25.96)%]and[(26.69-Z8.24)%],and C16:0[(5.89-29.15)%]and[(5.70-16.81)%].Several factors contribute to the nutritional value of *** the polyunsaturated FA content and n-3 to n-6 FA ratio,which could be used to assess the nutritional quality of ***:This study is the first comprehensive assessment of the FA composition and nutritional value of microalgae species in South Korea,and identifies the potential utility of FAs as species-specific biomarkers.

关键词： Microalgae PUFA Nutritive values DHA EPA

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Detection of coat protein gene of nervous necrosis virus using loopmediated isothermal amplification

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Asian Pacific Journal of Tropical Medicine 2016年第3期9卷 230-235页

作者： Jinik Hwang Sung-Suk Suh mirye park Myung-Joo Oh Jong-Oh Kim Sukchan Lee Taek-Kyun Lee South Sea Environment Research Department Korea Institute of ocean science & technology Korea University of Science and Technology Department of Aqualife Medicine Chonnam National University Department of Genetic Engineering Sungkyunkwan University

Objective:To establish a novel and highly specific loop-mediated isothermal amplification(LAMP) assay for the identification of nervous necrosis virus(NNV) ***:A set of synthesized primers was used to match the sequences of a specific region of the nnr gene from the National Center for Biotechnology Information database,not originating from NNV-infected fish,the efficiency and specificity of LAMP were measured dependent on the concentration of DNA polymerase and the reaction temperature and *** addition,to determine species-specific LAMP primers,cross reactivity testing was applied to the reaction between NVV and other virus families including viral hemorrhagic septicemia virus and marine ***:The optimized LAMP reaction carried out at 64 ℃ for 60 min,and above 4 U Bst DNA *** sensitivity of LAMP for the detection of nnv was thus about 10 times greater than the sensitivity of polymerase chain *** LAMP assay primers were specific for the detection NNV infection in Epinephelus ***:The development of LAMP primers based on genetic information from a public database,not virus-infected samples,may provide a very simple and convenient method to identify viral infection in aquatic organisms.

关键词： Nervous necrosis virus Nodaviridae Polymerase chain reaction Loop-mediated isothermal Amplification

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Omni-functional simultaneous interfacial treatment for enhancing stability and outgassing suppression of lithium-ion batteries

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Journal of Energy Chemistry 2024年第7期94卷 677-687页

作者： Youbean Lee Chanjoo park Kyoungmin Min Kwangjin park Department of Mechanical Engineering Gachon UniversityGyeonggi-do 13120Republic of Korea Department of Battery Engineering Gachon UniversityGyeonggi-do 13120Republic of Korea School of Mechanical Engineering Soongsil UniversitySeoul 06978Republic of Korea

Ni-rich layered oxides in lithium-ion batteries have problems with gas generation and electrochemical performance reduction due to residual lithium's reaction on the surface with the *** address this issue,in this study,the Acid solvent evaporation(AsE)method has been proposed as a potential method to remove residual lithium while promoting the formation of a new LiNO_(3)-derived coating layer on the cathode *** reduction of residual lithium using the ASE method and the construction of a LiNO_(3)-derived coating layer suppresses gas evolution caused by the side effects of the electrolyte,improves electrochemical performance,and improves thermal stability by facilitating the smooth movement of lithium ***,the structural stability and resistance change due to the LiNO_(3)-derived coating layer effects is guaranteed through cycling and DCIR of the pouch *** a result,compared to Pristine,the capacity retention of coin cells increased by 8%after 100 cycles,and pouch cells increased by 25%after 160 *** addition,after cycling the pouch cell,CO_(2) gas has significantly reduced by about 30%compared to Pristine using gas *** ASE method effectively forms a robust LiNO_(3)-derived coating layer on the cathode active material,which helps minimize electrolyte reactivity,suppress ,CO_(2) emissions,enhance surface structure stability,improve thermal stability,and improveoverallbatteryperformance.

关键词： Lithium-ion batteries Ni-rich NCM Acid solvent evaporation LiNO_(3)-derived coating Gasevolution

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Non-interleaved chiral metasurfaces and neural networks enhance the spatial resolution of polarimetry

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Light(Science & Applications) 2024年第3期13卷 396-398页

作者： Jaewon Jang Minsu park Yeonsang park Department of Physics Chungnam National UniversityDaejeon 34134Korea Institute of Quantum Systems Chungnam National UniversityDaejeon 34134Korea

Non-interleaved chiral metasurfaces for high-spatial-resolution polarimetry are proposed and ***,a convolutional neural network is incorporated to analyze interferometric images with the polarization state of light,an... 详细信息

关键词： parameters chiral resolution

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Iso-propagation vortices:a breakthrough in optical communication technology

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Advanced Photonics 2024年第3期6卷 13-15页

作者： Cherry park Junsuk Rho Pohang University of Science and Technology(POSTECH) Department of Mechanical EngineeringPohangRepublic of Korea Pohang University of Science and Technology(POSTECH) Department of Chemical EngineeringPohangRepublic of Korea Pohang University of Science and Technology(POSTECH) Department of Electrical EngineeringPohangRepublic of Korea POSCO-POSTECH-RIST Convergence Research Center for Flat Optics and Metaphotonics PohangRepublic of Korea

The rapid advance of optical communication technology requires improvements in data transmission capacity and *** methods of increasing the transmission capacity such as polarization,wavelength,and spatial mode division multiplexing have shown significant promise,while spatial mode division multiplexing(SDM)offers a new approach through the use of orthogonal spatial modes as distinct communication channels.

关键词： communication optical polarization

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Electrochemically Deposited CZTSSe Thin Films for Monolithic Perovskite Tandem Solar Cells with Efficiencies Over 17%

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Energy & Environmental Materials 2024年第1期7卷 147-152页

作者： Sun Kyung Hwang Ik Jae park Se Won Seo Jae Hyun park So Jeong park Jin Young Kim Department of Materials Science and Engineering Seoul National UniversitySeoul 08826Korea Department of Applied Physics Sookmyung Women's UniversitySeoul 04310Korea Research Institute of Advanced Materials and Systems Sookmyung Women's UniversitySeoul 04310Korea Research Institute of Advanced Materials(RIAM) Seoul National UniversitySeoul 08826Korea

In spite of the high potential economic feasibility of the tandem solar cells consisting of the halide perovskite and the kesterite Cu2ZnSn(S,Se)4(CZTSSe),they have rarely been demonstrated due to the difficulty in implementing solution-processed perovskite top cell on the rough surface of the bottom ***,we firstly demonstrate an efficient monolithic two-terminal perovskite/CZTSSe tandem solar cell by significantly reducing the surface roughness of the electrochemically deposited CZTSSe bottom *** surface roughness(R_(rms))of the CZTSSe thin film could be reduced from 424 to 86 nm by using the potentiostatic mode rather than using the conventional galvanostatic mode,which can be further reduced to 22 nm after the subsequent ion-milling *** perovskite top cell with a bandgap of 1.65 eV could be prepared using a solution process on the flattened CZTSSe bottom cell,resulting in the efficient perovskite/CZTSSe tandem solar *** the current matching between two subcells involving the thickness control of the perovskite layer,the best performing tandem device exhibited a high conversion efficiency of 17.5%without the hysteresis effect.

关键词： CZTSSe monolithic tandem solar cells perovskite solution process surface roughness control

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An Investigation on Open-RAN Specifications:Use Cases,Security Threats,Requirements,Discussions

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Computer Modeling in Engineering & Sciences 2024年第10期141卷 13-41页

作者： Heejae park Tri-HaiNguyen Laihyuk park Department of Computer Science and Engineering Seoul National University of Science and TechnologySeoul01811Republic of Korea Faculty of Information Technology School of TechnologyVan Lang UniversityHo Chi Minh City70000Vietnam

The emergence of various technologies such as terahertz communications,Reconfigurable Intelligent Surfaces(RIS),and AI-powered communication services will burden network operators with rising infrastructure ***,the Open Radio Access Network(O-RAN)has been introduced as a solution for growing financial and operational burdens in Beyond 5G(B5G)and 6G networks.O-RAN promotes openness and intelligence to overcome the limitations of traditional *** disaggregating conventional Base Band Units(BBUs)into O-RAN Distributed Units(O-DU)and O-RAN Centralized Units(O-CU),O-RAN offers greater flexibility for upgrades and network ***,this openness introduces new security challenges compared to traditional *** existing studies overlook these security requirements of the O-RAN *** gain deeper insights into the O-RAN system and security,this paper first provides an overview of the general O-RAN architecture and its diverse use cases relevant to B5G and 6G *** then delve into specifications of O-RAN security threats and requirements,aiming to mitigate security vulnerabilities *** providing a comprehensive understanding of O-RAN architecture,use cases,and security considerations,thisworkserves as a valuable resource for future research in O-RAN and its security.

关键词： O-RAN architecture use cases security issues security requirements security discussions

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