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Arp2/3 Complex Is Expressed in Oral Squamous Cell Carcinoma: An Immunohistochemical Study of 88 Cases

Open Journal of Stomatology 2019年第3期9卷 29-38页

作者： Marina P. Georgopoulou Konstantinos I. Tosios Nikolaos Goutas mirsini kouloukoussa Department of Forensic Medicine and Toxicology Medical School National and Kapodistrian University of Athens Athens Greece Department of Oral Medicine and Pathology Faculty of Dentistry National and Kapodistrian University of Athens Athens Greece Department of Histology and Embryology Medical School National and Kapodistrian University of Athens Athens Greece

Background: We investigated the immunohistochemical expression of Arp2/3 complex in 88 oral squamous cell carcinomas (OSCCs) and correlated it with the tumor type and grade. Materials and methods: This is a retrospective study of 88 cases of OSCCs (9 verrucous carcinomas, 4 carcinomas in situ, 37 well differentiated OSCCs, 32 moderately differentiated OSCCs, and 6 poorly differentiated OSCCs), stained with mouse monoclonal antibodies for Arp2 and Arp3. Staining was graded according to the extent of positivity by two observers. Results: Arp2 positivity was seen in 79 (89.77%) cases and Arp3 positivity in 78 (88.63%) cases. In positive cases, Arp2 expression was graded as (+) in 12.5%, (++) in 28.4% and (+++) in 48.86% of the cases;Arp3 was graded as (+) in 36.36%, (++) in 30.68% and (+++) in 21.59% of the cases. Conclusion: Expression of Arp2 and Arp3, indicative of the formation of the Arp2/3 complex, was seen in most OSCCs examined. Although no statistically significant correlation was found between the immunohistochemical expression of Arp2/Arp3 and tumor type or grade, the more common expression in invasive OSCCs than in non-invasive verrucous carcinomas and carcinomas in situ is indicative of its involvement in the biological behaviour of the lesions. The availability of Arp2/3 inhibitors that could be used in clinical practice warrants further study of the expression of Arp2/3 in OSCC.

关键词： Actin-Related Protein 2-3 Complex Squamous Cell Carcinoma Oral

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Action of the Disintegrin Contortrostatin on Breast Cancer Cell Primary Cultures

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Advances in Breast Cancer Research 2013年第4期2卷 161-169页

作者： Ifigeneia Marinou Sophia Havaki Nikos Goutas Dimitrios Vlachodimitropoulos Georgios Baltatzis Eumorphia G. Konstantakou Dimitrios J. Stravopodis Dimitrios G. Koudoumas Evangelos Marinos mirsini kouloukoussa 1st Department of Pathology Medical School University of Athens Athens Greece Department of Cell Biology and Biophysics Faculty of Biology University of Athens Athens Greece Laboratory of Experimental Surgery and Surgical Research University of Athens Athens Greece Laboratory of Forensic Medicine and Toxicology Medical School University of Athens Athens Greece Laboratory of Histology and Embryology Medical School University of Athens Athens Greece

Integrins mediate cell adhesion to the extracellular matrix (ECM). In particular, integrin alphavbeta3 recognizes the RGD motif as a ligand-binding site on various extracellular molecules of the extracellular matrix. Integrin aphavbeta3 has been associated with high malignant potential in breast cancer cells, and has signalized the onset of widespread metastasis. In recent years, several antagonists of integrin alphavbeta3, including snake venom disintegrins, have been used as potential anti-cancer agents. In the present work, the effect of contortrostatin, a disintegrin isolated from the venom of the snake Agkistrodon contortrix, was studied on primary cultures of human breast cancer cell. Scanning and transmission electron microscopy were employed in order to examine alterations in cell morphology and fluorescent microscopy and visualize changes in distribution of integrin alphavbeta3 and talin. Fluorescent localization of caspase 8 was made in order to visualize any sign of proapoptotosis and western immunoblotting of integrin, talin and annexin was undertaken in order to identify changes. The results suggest that the snake venom contortrostatin seriously affects cell morphology, adhesion and mobility and induces breast cancer cells to apoptosis.

关键词： Breast Cancer Disintegrins Contortrostatin Integrin Alphavbeta3 Apoptosis Primary Cultures

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