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Prevalence of Helicobacter pylori infection among patients with esophageal carcinoma

World Journal of gastroenterology 2024年第29期30卷 3479-3487页

作者： miriam lópez-gómez Maria Morales Rebeca Fuerte Marta Muñoz Pedro-David Delgado-lópez Jorge Francisco gómez-Cerezo Enrique Casado Department of Medical Oncology Precision Oncology LaboratoryInfanta Sofía University HospitalSan Sebastián de los Reyes 28231MadridSpain Department of Medical Oncology Infanta Sofía University HospitalSan Sebastián de los Reyes 28702Spain Department of Internal Medicine Infanta Sofía University HospitalSan Sebastián de los Reyes 28703MadridSpain Department of Pathology Infanta Sofía University HospitalSan Sebastián de los Reyes 28702Spain Department of Neurosurgery Burgos University HospitalBurgos 09006Spain Department of Internal Medicine Infanta Sofía University Hospital and Henares University Hospital Foundation for Biomedical Research and InnovationSan Sebastian de los Reyes 28702MadridSpain Department of Medical Oncology Infanta Sofia University Hospital and Henares University Hospital Foundation for Biomedical Research and InnovationSan Sebastian de los Reyes 28702MadridSpain

BACKgROUND Helicobacter pylori(***)is a widespread microorganism related to gastric adenocarcinoma(AC).In contrast,it has been reported that an inverse association exists between *** infection and esophageal *** mechanisms underlying this supposedly protective effect remain *** To determine the prevalence of *** infection in esophageal carcinoma patients,we performed a retrospective observational study of esophageal tumors diagnosed in our *** We retrospectively reviewed the prevalence of *** infection in a cohort of patients diagnosed with esophageal *** or previous proton pump inhibitor(PPI)usage was also *** A total of 89 patients with esophageal carcinoma(69 males,77.5%),with a mean age of 66 years(range,26-93 years)were *** was the most frequent pathological variant(n=47,52.8%),followed by squamous cell carcinoma(n=37,41.6%).Fourteen ACs(29.8%)originated in the gastroesophageal junction and 33(70.2%)in the esophageal ***,54 patients(60.7%)presented at stages III and *** *** infection occurred only in 4 patients(4.5%),3 with AC(6.3%of all ACs)and 1 with squamous cell carcinoma(2.7%of all squamous cell tumors).All patients with previous *** infection had stage *** one patient had received prior *** eradication therapy,whereas 86(96.6%)had received previous or concomitant PPI *** In our cohort of patients,and after histologic evaluation of paraffin-embedded primary tumors,we found a very low prevalence of previous *** *** also reviewed the medical history of the patients,concluding that the majority had received or were on PPI *** minimal prevalence of *** infection found in this cohort of patients with esophageal carcinoma suggests a protective role.

关键词： Helicobacter pylori Eradication Esophageal tumor Dysbiosis Proton pump inhibitors Carcinogenesis Microbiota Incidence

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gastrointestinal tumors and infectious agents:A wide field to explore

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World Journal of Meta-Analysis 2021年第6期9卷 505-521页

作者： miriam lópez-gómez Belén garcía de Santiago Pedro-David Delgado-lópez Eduardo Malmierca Jesús gonzález-Olmedo César gómez-Raposo Carmen Sandoval Pilar Ruiz-Seco Nora Escribano Jorge Francisco gómez-Cerezo Enrique Casado Medical Oncology Department.Precision Oncology laboratory Infanta Sofía University HospitalSan Sebastián de los Reyes 28231MadridSpain Pharmacy Department Infanta Sofia University HospitalSan Sebastián de los Reyes 28703MadridSpain Neurosurgery Department Burgos University HospitalBurgos 09006Spain Internal Medicine Department Infanta Sofía University HospitalSan Sebastián de los Reyes 28703MadridSpain Medical Oncology Department Infanta Sofia University HospitalSan Sebastián de los Reyes 28703MadridSpain Intensive Care Unit Jiménez Díaz FoundationMadrid 28040MadridSpain

Infection is currently one of the main contributors to *** fact,the International Agency for Research on Cancer has categorized eleven biological agents as group I *** is estimated that around 16%of the 12.7 million new cancers diagnosed in 2008 were attributable to infectious *** underdeveloped regions carry the highest incidence rates,about 7.4%of infectionrelated cancer cases occur in developed *** are increasingly aware of the potential carcinogenic role of common virus like the Human Papilloma virus in cervical cancer,or the hepatitis B and C viruses in ***,the carcinogenic role of several other infectious agents is less *** that gastrointestinal malignancies carry an overall poor prognosis,a better understanding of the carcinogenic mechanisms triggered by infectious agents is key to decrease the rate of cancer related *** measures directed to such infections would ideally impact *** this paper we review the main pathogenic mechanisms related to the development of gastrointestinal malignancies induced by infectious microorganisms and other pathogens which are currently under investigation.

关键词： gastrointestinal tumors Infectious agents Bacteria Virus Prevention

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Biomass gasification,catalytic technologies and energy integration for production of circular methanol:New horizons for industry decarbonisation

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Journal of Environmental Sciences 2024年第6期140卷 306-318页

作者： luis F.Bobadilla lola Azancot miriam gonzález-Castañ Estela Ruíz-lópez laura Pastor-Pérez Francisco J.Durán-Olivencia Runping Ye Katie Chong Paula HBlanco-Sánchez Zenthao Wu Tomás R.Reina JoséA.Odriozola Departamento de Química Inorgánica e Instituto de Ciencia de Materiales de Sevilla Centro Mixto CSIC-Universidad de SevillaAvda.Américo Vespucio 49Sevilla 41092Spain Departamento de Ingeniería Universidad Loyola AndalucíaAvda.de Las Universidades s/nSevilla 41704Spain Key laboratory of Jiangxi Province for Environment and Energy Catalysis School of Chemistry and Chemical EngineeringNanchang UniversityNanchang330031China Energy and Bioproducts Research Institute(EBRI) Aston UniversityBirminghamB47ETUnited Kingdom Department of Chemical and Process Engineering University of SurreyGuildfordGU27XHUnited Kingdom

The Intergovernmental Panel on Climate Change(IPCC)recognises the pivotal role of renewable energies in the future energy system and the achievement of the zero-emission *** implementation of renewables should provide major opportunities and enable a more secure and decentralised energy supply *** fuels provide long-term solutions for the transport sector,particularly for applications where fuels with high energy density are *** addition,it helps reducing the carbon footprint of these sectors in the *** on biomass characteristics feedstock is essential for scaling-up gasification from the laboratory to *** review deals with the transformation biogenic residues into a valuable bioenergy carrier like biomethanol as the liquid sunshine based on the combination of modified mature technologies such as gasification with other innovative solutions such as membranes and microchannel *** abatement is a critical process in product gas upgrading since tars compromise downstream processes and equipment,for this,membrane technology for upgrading syngas quality is discussed in this *** reactor technology with the design of state-of-the-art multifunctional catalysts provides a path to develop decentralised biomethanol synthesis from biogenic ***,the development of a process chain for the production of(i)methanol as an intermediate energy carrier,(ii)electricity and(iii)heat for decentralised applications based on biomass feedstock flexible gasification,gas upgrading and methanol synthesis is analysed.

关键词： Biogenic residues gasification Biomethanol Circular Economy Microreactors

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Multi-tissue transcriptomics of a unique monozygotic discordant twin case of severe progressive osseous heteroplasia

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genes & Diseases 2024年第3期11卷 22-25页

作者： Alberto gomez-Carballa Maria Jose Curras-Tuala Sara Pischedda miriam Cebey-lopez Jose gomez-Rial Irene Rivero-Cale Jacobo Pardo-seco Xabier Bello Sandra Viz-lasheras Antonio Justicia-grande Julian Montoto-louzao Alba Camino-Mera Isabel Ferreiros-Vidal Maximo Fraga Jose RAntunez Rodolfo gomez Federico Martinon-Torres Antonio Salas genetics Vaccines and Infections Research Group(GENVIP)Instituto de Investigacion Sanitaria de SantiagoSantiagode CompostelaGalicia 15706Spain Translational Pediatrics and Infectious Diseases Department of PediatricsHospital Clinico Universitario de Santiago de CompostelaServicio Galego de Saude(SERGAS)Galicia 15706Spain laboratorio de Inmunologia Servicio de Andlisis ClinicosHospital Clinico Universitario de Santiago de CompostelaServicio Galego de Saude(SERGAS)Galicia 15706Spain Unidade de Xenetica Instituto de Ciencias Forenses(INCIFOR)Facultade de MedicinaUniversidade de Santiago de Compostelaand GenPoB Research GroupInstituto de Investigacion SanitariaHospital Clinico Universitario de Santiago de CompostelaServicio Galego de Saude(SERGAS)Galicia 15706Spain Musculoskeletal Pathology group Instituto de Investigacion Sanitaria de SantiagoServicio Galego de Saude(SERGAS)Santiago de CompostelaGalicia 15706Spain Department of Pathology Hospital Clinico Universitario de Santiago de Compostela(SERGAS)Galicia 15706Spain Unidad Integrada de BiobancosBiomodelos 3D y Modelos Animales IDIS(lnstituto de Investigacion Sanitaria de Santiagode Compostela)Galicia15706Spain Centro de Investigacion Biomedica en Red de Enfermedades Respiratorias(CIBER-ES) MadridSpain

Progressive osseous heteroplasia(POH)is an ultra-rare autosomal dominant disabling disorder characterized by heterotopic ossification(HO).It is caused by heterozygous inactivating mutations in the gNAS(guanine nucleotide-binding protein alpha-stimulating activity polypeptide)***,the molecular mechanisms underlying HO remain poorly *** a treatment for POH is not yet available,the identification of the mechanisms driving POH in affected tissues using gene expression may be of great help to underestand the molecular basis of POH and develop new therapeutic approaches.

关键词： stimulating driving asi

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Effect of aging on behaviour of mesenchymal stem cells

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World Journal of Stem Cells 2019年第6期11卷 337-346页

作者： Juan Antonio Fafián-labora miriam Morente-lópez María C Arufe grupo de Terapia Celular y Medicina Regenerativa Departamento de Fisioterapia Ciencias Biomédicas y MedicinaUniversidade da Coru?a

Organs whose source is the mesoderm lineage contain a subpopulation of stem cells that are able to differentiate among mesodermal derivatives (chondrocytes, osteocytes, adipocytes). This subpopulation of adult stem cells, called “mesenchymal stem cells” or “mesenchymal stromal cells (MSCs)”, contributes directly to the homeostatic maintenance of their organs;hence, their senescence could be very deleterious for human bodily functions. MSCs are easily isolated and amenable their expansion in vitro because of the research demanding to test them in many diverse clinical indications. All of these works are shown by the rapidly expanding literature that includes many in vivo animal models. We do not have an in-depth understanding of mechanisms that induce cellular senescence, and to further clarify the consequences of the senescence process in MSCs, some hints may be derived from the study of cellular behaviour in vivo and in vitro, autophagy, mitochondrial stress and exosomal activity. In this particular work, we decided to review these biological features in the literature on MSC senescence over the last three years.

关键词： Mesenchymal stem cells Aging Autophagy Mitochondrial stress Extracellular vesicles

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Prevalence of hepatocarcinoma-related hepatitis B virus mutants in patients in grey zone of treatment

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World Journal of gastroenterology 2019年第38期25卷 5883-5896页

作者： Ana Isabel gil-garcía Antonio Madejón Irene Francisco-Recuero Ana lópez-lópez Emiliana Villafranca miriam Romero Araceli garcía Antonio Olveira Rocío Mena Juan Ramón larrubia Javier garcía-Samaniego Hepatology Unit Hospital Universitario La PazMadrid 28046Spain Centro de Investigación Biomédica en Red Instituto de Salud Carlos IIIMadrid 28029Spain Instituto de Investigación del Hospital Universitario la Paz Madrid 28046Spain Biochemistry Department Faculty of MedicineUniversidad Autónoma de MadridMadrid 28029Spain Instituto de genética Médica y Molecular Hospital Universitario La PazMadrid 28046Spain Translational Hepatology Unit Hospital General Universitario de GuadalajaraGuadalajara 19002Castilla-La ManchaSpain

BACKgROUND Antiviral treatment of patients with chronic hepatitis B(CHB)in the grey zone of treatment comands risk management in order to optimize the health *** this sense,the identification of HBV mutants related with an increased risk of hepatocellular carcinoma(HCC)could be useful to identify subpopulations with potential indication of antiviral *** To analyze the prevalence/persistence of hepatitis B virus(HBV)preS and basal core promoter(BCP)/precore/core variants associated to HCC development in CHB patients in the grey *** Work was designed as a longitudinal retrospective study,including 106 plasma samples from 31 patients with CHB in the grey zone of treatment:Hepatitis B e antigen negative,HBV-DNA levels between 12-20000 IU/ml,normal or discordant transaminase levels during follow up and mild/moderate necroinflammatory activity in liver biopsy or Fibroscan(up to 9.5 kPa).Serum HBVDNA was tested using the Abbott Real Time HBV Assay and the BCP/precore/core and the hepatitis B surface antigen(HBsAg)coding regions were analyzed in positive samples by PCR/bulk-sequencing to identify the HCCrelated HBV *** High-risk HCC related mutants were detected in 24(77%)patients:19(61%)in the BCP/precore/core,and 7(23%)in the HBsAg coding region(2 preS1 and 5 preS2 deletions).The prevalence of preS deletions was genotype-dependent:3/5(60%)patients with preS2 deletions and 1/2 with preS1 deletions were infected with the HBV-E *** HBV-E was the most prevalent in sub-Saharan patients,a correlation between preS deletions and ethnicity was also found:6/8(75%)sub-Saharan vs 1/19(5%)Caucasian patients had preS deletions(P=0.00016).Remarkably,this correlation was maintained in those patients infected with HBV-A,a minor genotype in sub-Saharan patients:2/2 patients infected with HBV-A from West Africa vs 0/6 of Caucasian origin had preS *** HCC related variants were the major strains and persisted over time(up to 48 mo).Pa

关键词： Hepatitis B virus Hepatocellular carcinoma PreS deletions Hepatitis B virus treatment grey zone

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Hepatocyte transplantation program:lessons learned andfuture strategies

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World Journal of gastroenterology 2016年第2期22卷 874-886页

作者： Eugenia Pareja Ibars miriam Cortes laia Tolosa Maria Joségómez-lechón Slivia lópez JoséVicente Castell JoséMir Unidad de Terapia Celular Hepatica Instituto de Investigación Sanitaria La FeHospital Universitario y Politécnico La Fe de Valencia46026 ValenciaSpain Unidad de Cirugía Hepato-Bilio-Pancreática y Trasplante Hepático Hospital Universitario y Politécnico La Fe de Valencia46026 ValenciaSpain liver transplantation Institute of Liver StudiesKing’s College HospitalInstitute of Liver StudiesSE5 9RS LondonUnited Kingdom Unidad de Hepatologıa Experimental Instituto de Investigación Sanitaria La FeHospital Universitario y Politécnico La Fe de Valencia46026 ValenciaSpain CIBERehd FIS08036 BarcelonaSpain

This review aims to share the lessons we learned over time during the setting of the hepatocyte transplantation(HT) program at the Hepatic Cell Therapy Unit at Hospital la Fe in Valencia. New sources of liver tissue for hepatocyte isolation have been explored. The hepatocyte isolation and cryopreservation procedures have been optimized and quality criteria for assessment of functionality of hepatocyte preparations and suitability for HT have been established. The results indicate that:(1) Only highly viable and functional hepatocytes allow to recover those functions lacking in the native liver;(2) Organs with steatosis(≥ 40%) and from elderly donors are declined since low hepatocyte yields, viability and cell survival after cryopreservation, are obtained;(3) Neonatal hepatocytes are cryopreserved without significant loss of viability or function representing high-quality cells to improve human HT;(4) Cryopreservation has the advantage of providing hepatocytes constantly available and of allowing the quality evaluation and suitability for transplantation; and(5) Our results from 5 adults with acute liver failure and 4 from children with inborn metabolic diseases, indicate that HT could be a veryuseful and safe cell therapy, as long as viable and metabolically functional human hepatocytes are used.

关键词： Hepatocyte transplantation Hepatocyteisolation Cell therapy Inborn errors of metabolism Neonatal hepatocytes Cryopreservation

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Sol-gel Silica Matrix as Reservoir for Controlled Release of Paracetamol: Characterization and Kinetic Analysis

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Journal of Encapsulation and Adsorption Sciences 2016年第2期6卷 47-55页

作者： Tessy lópez Mayra Álvarez Paola Ramírez gustavo Jardón miriam lópez guadalupe Rodriguez Itzel Ortiz Octavio Novaro laboratorio de Nanotecnología y Nanomedicina Universidad Autónoma Metropolitana-Xochimilco México D.F. México laboratorio de Nanotecnología Instituto Nacional de Neurología y Neurocirugía “MVS” México D.F. México Department of Chemical and Biomolecular Engineering Tulane University New Orleans USA Instituto de Física Universidad Nacional Autónoma de México Circuito de la Investigación s/n México D.F. México

Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) are usually suitable candidates for the development of drug delivery devices. Sol-gel chemistry represents an easy method to obtain porous silica nanoparticles. Mesoporous silica nanomaterials have been widely used for drug delivery purposes. In this work we synthesized silica based materials using two molar alkoxide:water ratios 1:4 and 1:8, incorporating paracetamol to develop a nano-sized matrix for controlled release purposes. The samples exhibited different values for surface area, porosity, particle size and distinct punctual defects. Infrared and UV-visible spectroscopic studies were carried out to demonstrate the effect of water concentration and the adequate incorporation of paracetamol molecules. Nitrogen adsorption characterization was realized and the estimated BET surface values were from 532 to 825 m2/g. Kinetic analysis of drug release profiles was performed using the hyperbola model. Transmission electron micrographs showed that all the materials formed aggregates of small particles with size between 10 - 60 nm. Mesoporous SiO2 materials were proved to be a suitable system for controlled release of paracetamol.

关键词： Sol-gel growth Nanostructures Biomaterials Surface Properties

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Type XIX collagen: a promising biomarker from the basement membranes

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Neural Regeneration Research 2020年第6期15卷 988-995页

作者： Ana C.Calvo laura Moreno leticia Moreno Janne M.Toivonen Raquel Manzano Nora Molina miriam de la Torre Tresa lópez Francisco J.Miana-Mena María J.Munoz Pilar Zaragoza Pilar larrodé Alberto garcía-Redondo Rosario Osta laboratory of genetics and Biochemistry(lAgENBIO) University of ZaragozaFaculty of Veterinary SciencesInstituto de Investigación Sanitaria de Aragón(IIS)IA2CIBERNEDZaragozaSpain Hospital Clínico Universitario lozano Blesa ZaragozaSpain Neurology Department ALS UnitCIBERER U-723Health Research InstituteMadridSpain

Among collagen members in the collagen superfamily,type XIX collagen has raised increasing interest in relation to its structural and biological *** XIX collagen is a Fibril-Associated Collagen with Interrupted Triple helices member,one main subclass of collagens in this *** collagen contains a triple helix composed of three polypeptide segments aligned in parallel and it is associated with the basement membrane zone in different *** molecular structure of type XIX collagen consists of five collagenous domains,COl1 to COl5,interrupted by six non-collagenous domains,NCI to *** most relevant domain by which this collagen exerts its biological roles is NCI domain that can be cleavage enzymatically to release matricryptins,exerting anti-tumor and anti-angiogenic effect in murine and human models of *** physiological conditions,type XIX collagen expression decreases after birth in different tissues although it is necessary to keep its basal levels,mainly in skeletal muscle and hippocampal and telencephalic interneurons in ***,in amyotrophic lateral sclerosis,altered transcript expression levels show a novel biological effect of this collagen beyond its structural role in basement membranes and its anti-tumor and anti-angiogenic *** XIX collagen can exert a compensatory effect to ameliorate the disease progression under neurodegenerative conditions specific to amyotrophic lateral sclerosis in transgenic SOD1 g93 A mice and amyotrophic lateral sclerosis *** novel biological role highlights its nature as prognostic biomarker of disease progression in and as promising therapeutic target,paving the way to a more precise prognosis of amyotrophic lateral sclerosis.

关键词： anti-tumor and anti-angiogenic properties C1 domain COl19A1 gene and protein levels compensatory effect FACIT collagens hippocampal interneurons matricryptins multiplexins NC1 domain regenerative response skeletal muscle type XIX collagen

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gOECP/SEOR radiotherapy guidelines for thymic epithelial tumours

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World Journal of Clinical Oncology 2021年第4期12卷 195-216页

作者： Mikel Rico Sonia Flamarique Cristina Casares Tamara garcía miriam lópez Maribel Martínez Javier Serrano Manuel Blanco Raúl Hernanz lourdes de Ingunza-Barón Francisco JoséMarcos Felipe Couñago Department of Radiation Oncology Complejo Hospitalario de NavarraPamplona 31008NavarraSpain Health Research Institute of Navarre(IdiSNA) Navarra BiomedPamplona 31008NavarraSpain Department of Radiation Oncology University Hospital Miguel ServetZaragoza 50009AragónSpain Department of Radiation Oncology University Hospital of CaceresCáceres 10004ExtremaduraSpain Department of Radiation Oncology Hospital Universitario de FuenlabradaFuenlabrada 28942MadridSpain Department of Radiation Oncology Hospital Clínico Universitario Lozano BlesaZaragoza 50009AragónSpain Department of Radiation Oncology Clínica Universidad de NavarraMadrid 28027Spain Department of Radiation Oncology Hospital Universitario TorrecárdenasAlmería 04009AndalucíaSpain Department of Radiation Oncology Hospital Universitario Ramón y CajalMadrid 28034Spain Department of Radiation Oncology Hospital Universitario Puerta del MarCádiz 11009AndalucíaSpain Department of Radiation Oncology Hospital Universitario Quirónsalud MadridHospital La LuzUniversidad Europea de MadridMadrid 28223Spain

Thymic epithelial tumours(TET)are rare,heterogeneous neoplasms that range from resectable indolent tumours to aggressive thymic carcinomas with a strong tendency to *** pathological diagnosis is complex,in part due to the management of TETs is scant and mainly based on non-randomised studies and retrospective ***,the clinical management of TETs tends to be highly heterogenous,which makes it difficult to improve the evidence *** role of technological advances in the field of radiotherapy and new systemic therapies in the treatment of TETs has received little attention to *** the present clinical guidelines,developed by the gOECP/SEOR,we review recent developments in the diagnosis and classification of *** also present a consensus-based therapeutic strategy for each disease stage that takes into consideration the best available *** guidelines focus primarily on the role of radiotherapy,including recent advances,in the management of *** main aim of this document is to promote the standardisation of clinical practice and lay the foundations for future studies to clarify the main unresolved questions related to the optimal management of TET.

关键词： Targeted therapies Thymic epithelial tumors Radiotherapy Reirradiation guidelines Chemotherapy

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