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Adverse Events following AstraZeneca COVID-19 Vaccination: A Case Study in Abidjan, Côte d’Ivoire

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Open Journal of Immunology 2024年第2期14卷 11-15页

作者： Chiayé Claire Antoinette Yapo-Crezoit Samuel Boahene mireille Dosso Immunity Biology Unit Pasteur Institute of Cte dIvoire Abidjan Cte dIvoire Pasteur Institute of Cte dIvoire Abidjan Cte dIvoire

Introduction: Pharmaceutical companies have boosted vaccine production following the global COVID-19 pandemic. In Côte d’Ivoire, the first vaccination campaign with the AstraZeneca vaccine began on March 1, 2021, as part of the Covax program. Despite the positive benefit/risk balance, the adverse effects of vaccination should not be minimized. Objective: To identify adverse events of AstraZeneca’s COVID-19 vaccination for better management. Materials and Methods: This is a case of a 57-year-old obese (BMI = 39 kg/m2) female health care worker who experienced adverse events in March 2021 after the second dose of AstraZeneca vaccine administered 4 weeks apart. These were subject to mandatory case reporting. Results: Major post-vaccination events occurred in a noisy systemic picture with parameters showing significant disturbances. Biological surveillance remains costly and makes the accountability of the vaccine complex. Conclusion: Vaccination remains the ultimate weapon in the fight against endemic diseases but should not overshadow the reporting of adverse events.

关键词： Adverse Events Post-Second Immunization AstraZeneca Vaccine Abidjan

Insecticidal Activity of Bacillus thuringiensison Larvae and Adults of Bactrocera oleae Gmelin(Diptera:Tephritidae)

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Journal of Environmental Protection 2013年第5期4卷 480-485页

作者： Faiza Ilias Nassira Gaouar Kanza Medjdoub mireille Kallassy Awad Faculty of Science Saint- Joseph University Beirut Lebanon Laboratory of Ecology and Management of Natural Ecosystems Tlemcen University Tlemcen Algeria

The olive fruit fly Bactrocera oleae is a major olive pest in Algeriaand other Mediterranean countries. Its attacks, in some cases, seriously compromised production. Bacillus thuringiensis is a spore-forming soil bacterium which produces a protein crystal toxic to some insects. The objective of this study was to search for isolates toxic to larvae and adults of B. oleae. Four doses test were performed on neonate larvae, second instar larvae and third instar larvae. Seven Bt strains examined, showed highest toxicity levels against adults. 86% mortality was observed against neonate larvae after 7 days of application by Bt toxin isolate. The mortality of the second instar larvae was 65% mortality at dose of 108 UFC/mL. The third instar larvae was very susceptible to Bt strain with 70% of mortality. This study demonstrated that some local Algerian B. thuringiensis isolates exhibit toxic potential that could be used to control B. oleae.

关键词： Bacillus thuringiensis Bactrocera oleae Insecticidal Activity Olive fly Diptera

Effect of Robusta (Coffea canephora P.) Coffee Cherries Storage after Harvest before Putting Out for Sun Drying on Development of Toxigenic Fungi and the Variation of the Physicochemical Components

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Food and Nutrition Sciences 2014年第2期5卷 117-126页

作者： Irene Ahou Kouadio Mathias Kouame Koffi mireille Bretin Dosso Laboratory of Biochemistry and Food Sciences UFR Biosciences University Felix Houphouet-Boigny Abidjan Ivory Coast Pasteur Institute of Ivory Coast Abidjan Cote d’Ivoire

In this study, the effects of the storage duration of coffee cherries after harvest before putting out for sun drying on the kinetics of drying, fungi development and the variation of physicochemical content were evaluated. The results showed that the longer coffee cherries were stored after harvest before putting out for sun drying, the quicker they dried. Indeed, the drying durations were 19, 16, 12, 10, 7 days respectively for coffee cherries put out for sun drying at the day of harvest, the second, the fourth, the sixth and the eighth day after harvest. However, this storage of the cherries after harvest before putting out for sun drying led to the increasing to the infection of cherries by fungi. Indeed, samples of more contaminated inside were those from the lots of cherries stored 8 days after harvest before putting out for sun drying with 55.55% of the samples infected with a percentage of infected beans between 10% and 50%, and 44.45% of the samples were infected with a percentage of infected beans between 50% and 100%. Furthermore, those put out for sun drying at the day of harvest were free inside by fungi. Among the fungi isolated, toxigenic species was found. However, no relationship between the frequencies of ochratoxin A producing strains isolated and the storage duration of the cherries after harvest before putting out for sun drying was noted. This storage of the cherries after harvest before putting out for sun drying also led to the acidification of the cherries (pH = 5.27 - 3.6) and the degradation of their chlorogenic acids content (12.58% - 10.30%) while for their caffeine content (2.53% - 2.55%). No significant difference was observed about the storage duration of the cherries after harvest before putting out for sun drying.

关键词： Coffee Cherries Fungi Aspergillus Toxigenic Physicochemical Components

Pre-fabricated radial forearm phalloplasty with cadaveric bone graft

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World Journal of Clinical Urology 2014年第3期3卷 376-379页

作者： Jason W edens Tuan Tran Sarah Eidelson Morad Askari Christopher J Salgado Division of Plastic Surgery Department of Surgery Miller School of Medicine University of Miami and Jackson Memorial HospitalMiami FL 33136 United States

Phalloplasty is a complex set of procedures used in efforts to improve the anatomical,physiological,and aesthetic deficiencies caused by loss or absence of the *** have evolved significantly,and the use of free tissue transfer has become common amongst reconstructive *** inclusion of bone autograft,usually radius or fibula,within the neophallus has caused significant morbidity,and efforts continue to find the optimal *** present a novel approach using a pre-fabricated,radial forearm fasciocutaneous free flap containing cadaveric bone graft for phalloplasty following traumatic penis amputation.

关键词： Phalloplasty Pre-fabrication Cadaver allograft Radial forearm Penile reconstruction

Prevalence and Sensitization and Intestinal Parasitic Infestation of Vernal Keratoconjunctivitis in School Children of Kinshasa

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Open Journal of Pediatrics 2023年第4期13卷 568-580页

作者： Léon Muamba Nkashama David Kayembe Lubeji mireille Solange Nganga Nkanga Ophthalmology Department University Hospital of Kinshasa Kinshasa Democratic Republic of Congo Clinical Biology Department University Hospital of Kinshasa Kinshasa Democratic Republic of Congo

Background: There is no study that estimates the prevalence of vernal keratoconjunctivitis and the association between vernal keratoconjunctivitis and intestinal parasitic infestation in our setting. Purpose: This study aimed to estimate the vernal keratoconjunctivitis prevalence, describe the profile of sensitization and determine the association between vernal keratoconjunctivitis disease and intestinal parasitic infestation in school children of Kinshasa during the scholar year from 2022 to 2023. Methods: An observational, cross-sectional study was performed from September 2022 to June 2023. The children with vernal keratoconjunctivitis were recruited by using the four-stage probability sampling technique. Children attending in the school children of Kinshasa with vernal keratoconjunctivitis were enrolled in the classroom and underwent a clinical examination and skin prick test for 11 allergens and stool examination direct with microscope in the Laboratory. Data were entered in Microsoft Sheets using SPSS version 20.0. The Chi-square test was used to compare the sex, age groups, home environment (grow trees and/or flowers around the yard), dampness in the house, keeping pets (cat and/or dog), number of people per household, number of people sharing a bedroom and intestinal parasitic infestation. The significant level was set to a value of p Results: A total of 28,800 students from four-stage probabilist sampling technique in school of Kinshasa were included, and 2100 students had symptoms according to the vernal keratoconjunctivitis-related symptom questionnaire. Children with vernal keratoconjunctivitis were enrolled and the mean age ± standard was 7.2 ± 3.7 years, with a prevalence of 7.2% and gender was three boys for one girl. All vernal keratoconjunctivitis children recruited in urban and rural part had 33.1% positive skin prick test (SPT) and 67.9% showed polysensitization. Children an urban part was more sensitized than rural respectively with Dermatophago

关键词： Prevalence Sensitization Intestinal Parasitic Infestation School Children Kinshasa

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数据包保护专家

科技纵览 2017年第4期 42-48页

作者： Glenn edens Glenn Scott

互联网发展至今已有45年之久,老态初现。可以肯定的是，它为我们提供了无与伦比的服务。其基础技术为我们提供了万维网（年约28岁，还称得上是青年）和全球通信网络。互联网用户基础已经达34亿，这些技术也相应地扩大了其规模。

关键词：数据包互联网用户专家保护通信网络万维网技术基础

PCR-HRM for Genomic Surveillance of SARS-CoV-2: A Variant Detection Tool in Côte d’Ivoire, West Africa

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American Journal of Molecular Biology 2024年第3期14卷 166-185页

作者： Aboubacar Sylla Solange Kakou-Ngazoa Tata Gniré Safiatou Coulibaly Yakoura Karidja Ouattara mireille Sylvie Kouamé-Sina Zeinab Ouattara David Ngolo Coulibaly Brice Kouakou Bla mireille Dosso Molecular Biology Platform Pasteur Institute Cte dIvoire Abidjan Cte dIvoire Laboratory of Biology and Health UFR Biosciences University Flix Houphout Boigny Abidjan Cte dIvoire Genetic and Cancer Laboratory Molecular Genetics Platform Pasteur Institute Cte dIvoire Abidjan Cte dIvoire Epidemic Virus Department Pasteur Institute Cte dIvoire Abidjan Cte dIvoire

The rise of new viruses, like SARS-CoV-2 causing the COVID-19 outbreak, along with the return of antibiotic resistance in harmful bacteria, demands a swift and efficient reaction to safeguard the health and welfare of the global population. It is crucial to have effective measures for prevention, intervention, and monitoring in place to address these evolving and recurring risks, ensuring public health and international security. In countries with limited resources, utilizing recombinant mutation plasmid technology in conjunction with PCR-HRM could help differentiate the existence of novel variants. cDNA synthesis was carried out on 8 nasopharyngeal samples following viral RNA extraction. The P1 segment of the SARS-CoV-2 Spike S protein was amplified via conventional PCR. Subsequently, PCR products were ligated with the pGEM-T Easy vector to generate eight recombinant SARS-CoV-2 plasmids. Clones containing mutations were sequenced using Sanger sequencing and analyzed through PCR-HRM. The P1 segment of the S gene from SARS-CoV-2 was successfully amplified, resulting in 8 recombinant plasmids generated from the 231 bp fragment. PCR-HRM analysis of these recombinant plasmids differentiated three variations within the SARS-CoV-2 plasmid population, each displaying distinct melting temperatures. Sanger sequencing identified mutations A112C, G113T, A114G, G214T, and G216C on the P1 segment, validating the PCR-HRM findings of the variations. These mutations led to the detection of L452R or L452M and F486V protein mutations within the protein sequence of the Omicron variant of SARS-CoV-2. In summary, PCR-HRM is a vital and affordable tool for distinguishing SARS-CoV-2 variants utilizing recombinant plasmids as controls.

关键词： Genomic Surveillance SARS-CoV-2 PCR-HRM Variants Côte d’Ivoire

Selection of RNAi-based inhibitors for anti-HIV gene therapy

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World Journal of Virology 2012年第3期1卷 79-90页

作者： Stefanie A Knoepfel mireille Centlivre Ying Poi Liu Fatima Boutimah Ben Berkhout Laboratory of Experimental Virology Department of Medical Microbiology Center for Infection and Immunity Amsterdam Academic Medical Center University of Amsterdam 1105 AZ Amsterdam The Netherlands

In the last decade, RNA interference(RNAi) advanced to one of the most widely applied techniques in the biomedical research field and several RNAi therapeutic clinical trials have been launched. We focus on RNAibased inhibitors against the chronic infection with human immunodeficiency virus type 1(HIV-1). A lentiviral gene therapy is proposed for HIV-infected patients that will protect and reconstitute the vital immune cell pool. The RNAi-based inhibitors that have been developed are short hairpin RNA molecules(sh RNAs), of which multiple are needed to prevent viral escape. In ten distinct steps, we describe the selection process that started with 135 sh RNA candidates, from the initial design criteria, via testing of the in vitro and in vivo antiviral activity and cytotoxicity to the final design of a combinatorial therapy with three sh RNAs. These sh RNAs satisfied all 10 selection criteria such as targeting conserved regions of the HIV-1 RNA genome,exhibiting robust inhibition of HIV-1 replication and having no impact on cell physiology. This combinatorial sh RNA vector will soon move forward to the first clinical studies.

关键词： Human immunodeficiency virus type 1 RNA interference Gene therapy “Human Immune System”mouse Lentivirus

Hybrid Successive CFA Image Encryption-Watermarking Algorithm Based on the Quaternionic Wavelet Transform (QWT)

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Journal of Information Security 2022年第4期13卷 244-256页

作者： Hervé Abena Ndongo Marcelin Vournone mireille Pouyap Taya Ngakawa Paul Abena Malobe Energy Signal Imaging and Automatic Laboratory (LESIA) Department of Electrical and Industrial Automatic Engineering National School of Agro-Industrial Sciences (ENSAI) University of Ngaoundere Ngaoundere Cameroon Mapping Production Division Department of Data Collection National Institute of Cartography Yaounde Cameroon Department of Physics Higher Teacher Training College University of Maroua Maroua Cameroon Department of Physics Faculty of Science (FS) University of Ngaoundere Ngaoundere Cameroon

In this paper, we present a new robust hybrid algorithm combining successively chaotic encryption and blind watermarking of images based on the quaternionic wavelet transform (QWT) to ensure the secure transfer of digital data. The calculations of the different evaluation parameters have been performed in order to determine the robustness of our algorithm to certain attacks. The application of this hybrid algorithm on CFA (Color Filter Array) images, allowed us to guarantee the integrity of the digital data and to propose an autonomous transmission system. The results obtained after simulation of this successive hybrid algorithm of chaotic encryption and then blind watermarking are appreciated through the values of the evaluation parameters which are the peak signal-to-noise ratio (PSNR) and the correlation coefficient (CC), and by the visual observation of the extracted watermarks before and after attacks. The values of these parameters show that this successive hybrid algorithm is robust against some conventional attacks.

关键词： Encryption Blind Watermarking Chaos CFA Images QWT