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Treatment Effects of Acupuncture and Calligraphy Training on Cognitive Abilities in Senile Demented Patients

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Chinese Medicine and Culture 2019年第2期2卷 95-98页

作者： Henry S.R.Kao Miqu Wang Shuguang Yu Shihong Yuan miranda MY Fung lin Zhu Stewart P.W.Lam Tin Tin Kao Xiaoyang Kao Departments of Psychology 9Psychology and GeographyUniversity of Hong KongHKSAR Calligraphy Therapy Research Laboratory Shenzhen Institute of NeuroscienceShenzhen Institute of Psychology and Genetics of Chinese Medicine School of Acup uncture and Tuina Chengdu University of Traditional Chinese MedicineChengdu Department of Medicine Shanxi Medical College for Continuing Education Beijing Tong Ren Tang Chinese Medicine Clinic Beijing Tong Ren Tang Taiyuan Chinese Medicine Hospital Department of Chinese Medicine China Academy of Chinese Medical Sciences Division of Research Advocacy 21^st Century Education Research InstituteBeijingChina 不详 Department of Electrical Engineering University of Illinois at Urbana-ChampaignChampaignIllinoisUSA

Purpose:This study compared the relative effectiveness of Chinese calligraphy handwriting(CCH)and acupuncture in the treatment of patients with senile *** and Methods:A randomized controlled trial(RCT)with 17 mild-to-moderate dementia patients with an average age of 77.29 years were randomly assigned with 9 to the calligraphy handwriting group and 8 to the acupuncture treatment group for a month of consecutive *** participants'cognitive abilities,as well as symptoms of senile dementia,were measured by the Chinese version of the Mini-mental State Examination(CMMSE)and the Chinese Medicine Quantitative Diagnostic Survey for Senile Dementia Symptoms,respectively,before and after the ***:The calligraphy group showed a significant increase in calculation and memory as well as a decline in the symptoms of senile *** in the acupuncture group experienced a significant growth in total CMMSE scores and the subscales in orientation to time and place,behavioral operations,as well as reduced clinical ***,no significant changes were found in their memory and calculation ***:Both CCH and acupuncture treatments were found significantly effective for,respectively,enhancing the patients'cognitive abilities and reducing their clinical ***,calligraphy handwriting also improved the level of their attention and concentration,physical relaxation,and emotional stability.

关键词： Acupuncture calligraphy handwriting cognitive abilities senile dementia

On the Normality of Meromorphic Functions

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Journal of Mathematical Research with Applications 2014年第1期34卷 89-96页

作者： Guobin lin Institute of Minnan Science and Technology Fujian Normal University

In this paper,we deal with the problem of normal families concerning *** results extend and improve some theorems put forward by miranda,Pang,Chen,Hua and ***,we answer one question posed by Gu,Pang,Fang and so on.

关键词： meromorphic normality shared-value.

MicroRNA expression profiling and bioinformatics analyses of Dengue virus-infected peripheral blood mononuclear cells

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MicroRNA expression profiling and bioinformatics analyses of...

子痫前期患者脐带血循环内皮细胞集落形成细胞水平降低

第八届全国免疫学学术大会

作者： YIMING QI YING LI lin ZHANG JUNQI HUANG Institute of Immunology Zhongshan School of Medicine Sun Yat-sen University and Key Laboratory of Tropical Diseases Control Ministry of Education

Dengue virus(DENV) can cause self–limiting dengue fever(DF) and severe dengue hemorrhagic fever(DHF) and dengue shock syndrome(DSS).It is generally believed that cytokine storm leads to the increased plasma leakage characteristic of DHF/*** this study, peripheral blood mononuclear cells(PBMCs) were isolated from blood samples of healthy volunteers, and infected with DENV serotype 2(DENV2).Culture supernatants of DENV2-infected and uninfected PBMCs were tested against a human cytokine *** 6-12 h post-infection, levels of CCL5, IL-6, IL-8 were remarkably elevated, while those of TNF-α *** RNA isolated from these PBMCs was used against a human miRNA microarray to identify differentially expressed microRNAs(miRNAs).11 upregulated and 4 downregulated miRNAs were valided by q *** analyses of Sanger mibase, miranda and Target Scan, we found 261 common predicted *** were used to identify homologous sequences on mRNAs of putative target genes that may be directly bound by the miRNAs identified above and we found cytokines and epigenetic regulators might be putative target genes of these miR *** the analyses of Ingenuity Pathway Analysis(IPA), we noticed that canonical pathways like biological regulation pathway which were predicted to be highly modulated by these miRNA

关键词： dengue virus peripheral blood mononuclear cells microRNAs bioinformatics analyses

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中华高血压杂志 2014年第5期22卷 444-444页

作者：刘莉叶鹏 Muoz-Hernandez R miranda ML Stiefel P lin RZ Praena-Fernández JM Dominguez-Simeon MJ Villar J Moreno-Luna R Melero-Martin JM

子痫前期是一种与后代心血管危险因素增加有关的妊娠相关性疾病。内皮细胞集落形成细胞（endo-thelialcolony-formingcells，ECFCs）是生长过程中参与血管形成的循环内皮祖细胞的一部分。然而，子痫前期对于胎儿ECFCs水平的影响尚未知。

关键词：子痫前期细胞水平内皮细胞细胞集落形成血循环心血管危险因素脐带患者

Analysis of long noncoding RNA-associated competing endogenous RNA network in glucagon-like peptide-1 receptor agonist-mediated protection inβcells

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World Journal of Diabetes 2020年第9期11卷 374-390页

作者： Li-Juan Cui Tao Bai lin-Ping Zhi Zhi-Hong Liu Tao Liu Huan Xue Huan-Huan Yang Xiao-Hua Yang Min Zhang Ya-Ru Niu Yun-Feng Liu Yi Zhang Department of Pharmacology Basic Medical CollegeShanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Endocrinology The First Clinical Hospital of Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Respiratory Medicine The First Clinical Hospital of Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of General Surgery Shanxi Bethune HospitalTaiyuan 030006Shanxi ProvinceChina College of Pharmacy Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Second Clinical Medical College Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina

BACKGROUND Long noncoding RNAs(lncRNAs)and mRNAs are widely involved in various physiological and pathological *** use of glucagon-like peptide-1 receptor agonists(GLP-1RAs)is a novel therapeutic strategy that could promote insulin secretion and decrease the rate ofβ-cell apoptosis in type 2 diabetes mellitus(T2DM)***,the specific lncRNAs and mRNAs and their functions in these processes have not been fully identified and *** To identify the lncRNAs and mRNAs that are involved in the protective effect of GLP-1RA inβcells,and their *** Rat gene microarray was used to screen differentially expressed(DE)lncRNAs and mRNAs inβcells treated with geniposide,a *** Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed to assess the underlying functions of DE *** mRNAs were filtered using the STRING database and the Cytoscape plugin,*** order to reveal the regulatory relationship between lncRNAs and hub mRNAs,their co-expression network was constructed based on the Pearson coefficient of DE lncRNAs and mRNAs,and competing endogenous RNA(ceRNA)mechanism was explored through miranda and TargetScan *** We identified 308 DE lncRNAs and 128 DE mRNAs with a fold change filter of≥1.5 and P valueling pathway,inflammatory response,calcium signaling pathway,positive regulation of cell proliferation,and ERK1 and ERK2 ***,Htr2a,and Agtr1a were screened as hub mRNAs using the STRING database and the Cytoscape plugin,*** result was further verified using SwissTargetPrediction *** the co-expression network and competing endogenous(ceRNA)mechanism,we identified seven lncRNAs(NONRATT027738,NONRATT027888,NONRATT030038,etc.)co-expressed with the three hub mRNAs(Pomc,Htr2a,and Agtr1a)based on the Pearson coefficient of

关键词： Type 2 diabetes βcell Long noncoding RNA Competing endogenous RNA Co-expression analysis Glucagon-like peptide-1 receptor agonist

miR-29b-3p靶向调控STAT3对AML细胞增殖及凋亡的影响

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中国实验血液学杂志 2020年第6期28卷 1853-1858页

作者：林梨平张倩吴玮薛龑唐永金林东红福建医科大学医学技术与工程学院福建福州350004

目的:探讨miR-29b-3p通过靶向调控信号转导和转录激活因子3(STAT3)对急性髓系白血病(AML)细胞增殖及凋亡的影响。方法:TargetScan及miranda在线数据库预测miR-29b-3p与STAT33'UTR区的结合位点,采用双荧光素酶报告基因实验检测二者的靶... 详细信息

目的:探讨miR-29b-3p通过靶向调控信号转导和转录激活因子3(STAT3)对急性髓系白血病(AML)细胞增殖及凋亡的影响。方法:TargetScan及miranda在线数据库预测miR-29b-3p与STAT33'UTR区的结合位点,采用双荧光素酶报告基因实验检测二者的靶向关系。qPCR及Western blot检测慢病毒过表达miR-29b-3p后AML细胞miR-29b-3p与STAT3 mRNA及蛋白表达变化,采用MTS检测各组AML细胞增殖变化,流式细胞术检测细胞凋亡情况。结果:STAT3的3'UTR区存在与miR-29b-3p的结合位点,双荧光素酶报告基因实验证实STAT3是miR-29b-3p的靶基因。慢病毒过表达miR-29b-3p后,STAT3 mRNA及蛋白表达降低,过表达组AML细胞增殖减缓且凋亡增加(P<0.05)。结论:miR-29b-3p可靶向下调STAT3从而抑制AML细胞增殖,诱导细胞凋亡。

关键词：急性髓系白血病 miR-29b-3p 信号转导和转录激活因子3 细胞增殖细胞凋亡

LncRNA SLC16A1-AS1靶向调控miR-182对乳腺癌BT549细胞增殖能力的影响

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临床与实验病理学杂志 2023年第8期39卷 909-914页

作者：蒋冰马琳刘骞辽宁省肿瘤医院病理科沈阳110042

目的探讨LncRNA SLC16A1-AS1对miR-182的靶向调控作用及对乳腺癌BT549细胞增殖能力的影响。方法使用GEPIA2工具分析TCGA数据库中LncRNA SLC16A1-AS1在三阴型乳腺癌(triple negative breast cancer,TNBC)中的差异性表达,应用Kaplan-MeieRPlotter数据库进行生存分析。利用RegRNA2和miranda数据库预测LncRNA SLC16A1-AS1的潜在靶标miRNAs。用qRT-PCR检测LncRNA SLC16A1-AS1在TNBC组织及细胞系(BT549、BT20、MDA-MB-231及MDA-MB-468)中的表达。利用双荧光素酶活性测定及RNA免疫沉淀实验验证LncRNA SLC16A1-AS1与miR-182的结合力。用CCK-8及集落形成实验检测不同处理组BT549细胞增殖能力的变化。结果生物信息学分析结果显示,TNBC组织中LncRNA SLC16A1-AS1的表达水平显著低于正常组织(P<0.001),与预后良好相关(P<0.001),LncRNA SLC16A1-AS1与miR-182之间存在结合位点。LncRNA SLC16A1-AS1在63例TNBC组织中的表达水平显著低于瘤旁组织(7.94%vs 63.49%,P<0.001),在TNBC细胞系中的表达水平亦显著低于正常乳腺上皮细胞系(P<0.01)。在BT549细胞中过表达miR-182可显著降低LncRNA SLC16A1-AS1-WT载体的荧光酶素活性,RNA免疫沉淀实验显示,AGO2同时富集LncRNA SLC16A1-AS1及miR-182(P<0.01)。与对照组相比,过表达LncRNA SLC16A1-AS1显著降低BT549细胞的增殖活性并减少集落形成数量,共表达LncRNA SLC16A1-AS1与miR-182可恢复BT549细胞的增殖能力(P<0.01)。结论LncRNA SLC16A1-AS1在TNBC中低表达,且通过靶向调控miR-182在体外抑制TNBC细胞的增殖能力,LncRNA SLC16A1-AS1/miR-182轴有望成为TNBC治疗的潜在靶点。

关键词：乳腺肿瘤三阴型乳腺癌 LncRNA SLC16A1-AS1 miR-182

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脑神经胶质瘤替莫唑胺耐药circRNA-miRNA-mRNA调控网络的构建

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安徽医药 2021年第7期25卷 1414-1418页

作者：朱琳长沙市第四医院神经内科湖南长沙421001

目的筛选脑神经胶质瘤替莫唑胺耐药细胞株U87/TR与亲本细胞株U87的环状RNA(circRNA)差异表达谱以及构建关键环状RNA(circRNA)-微小RNA(miRNA)-信使RNA(mRNA)调控网络。方法2020年1—6月,应用circRNAs芯片检测神经胶质瘤替莫唑胺耐药细胞... 详细信息

目的筛选脑神经胶质瘤替莫唑胺耐药细胞株U87/TR与亲本细胞株U87的环状RNA(circRNA)差异表达谱以及构建关键环状RNA(circRNA)-微小RNA(miRNA)-信使RNA(mRNA)调控网络。方法2020年1—6月,应用circRNAs芯片检测神经胶质瘤替莫唑胺耐药细胞circRNAs差异表达谱,并进行RT-PCR验证。用miranda数据库预测circRNA-miRNA靶向结合关系,通过miranda v5、TargetScan、miBase数据库预测靶基因,Cytoscape软件构建circRNA-miRNA-mRNA调控网络。结果circRNA芯片结果显示,与U87细胞比较,U87/TR细胞有313个差异有统计学意义表达的circRNAs(P<0.05),其中145个显著上调,168个明显下调;RT-qPCR验证结果与芯片结果相符。miRNA靶位点预测结果显示,关键上调人环状RNAhsa_circRNA_009054,hsa_circRNA_104338(n=3,t=12.09、9.52,P=0.007、0.011)以及下调hsa_circRNA_016459,hsa_circRNA_101975(n=3,t=13.86、7.75,P=0.005、0.016)可与hsa-miR-33a-5p,hsa-miR-15b-3p以及hsa-miR-193b-5p,hsa-miR-23a-5p等多个miRNAs靶向结合,关键circRNA-miRNA-mRNA调控网络包括4个关键circRNAs,20个关键miRNAs,以及278个关键mRNAs。结论circRNA在脑神经胶质瘤替莫唑胺耐药细胞中异常表达,hsa_circRNA_009054,hsa_circRNA_104338以及hsa_circRNA_016459,hsa_circRNA_101975等关键circRNA可能通过circRNA-miRNA-mRNA调控网络参与脑神经胶质瘤替莫唑胺的耐药过程。

关键词：神经胶质瘤替莫唑胺抗药性,肿瘤 circRNA差异表达谱 circRNA-miRNA-mRNA调控网络

Altered expression profiles of microRNAs in a stable hepatitis B virus-expressing cell line

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Chinese Medical Journal 2009年第1期122卷 10-14页

作者： LIU Yan ZHAO Jian-Jun WANG Chun-mei LI Mian-yang HAN Ping WANG lin CHENG Yong-qian Fabien Zoulim MA Xu XU Dong-ping Viral Hepatitis Research Laboratory institute of Infectious Diseases Beijing 302 Hospital Beijing 100039 China Department of Genetics National Research Institute for Family Planning Peking Union Medical College Tsinghua University Beijing 100081 China Clinical Laboratory General Hospital of the People's Liberation Army Beijing 100853 China Department of Liver Diseases Hospices Civils de Lyon Hotel Dieu 69002 Lyon France

Background MicroRNAs （miRNAs） are highly conserved small non-coding RNAs of 18-25 nucleotides （nt） that mediate post-transcriptional gene regulation. Hepatitis B virus （HBV） can cause either acute or chronic hepatitis B, and is a high risk factor for liver cirrhosis and hepatocellular carcinoma. Some mammalian viruses have been shown to modulate the expression of host cellular miRNAs. However, interactions between the HBV and the host cellular miRNAs are largely unknown.Methods miRNA microarray and Northern blotting analysis were used to compare the expression profile of cellular miRNAs of a stable HBV-expressing cell line HepG2.2.15 and its parent cell line HepG2. mRNA microarray assay and the miranda program were used to predict the miRNA targets. A flow cytometric assay was further used to investigate the expression of human leukocyte antigen （HLA）-A.Results Eighteen miRNAs were differentially expressed between the two cell lines. Among them, eleven were up-regulated and seven were down-regulated in HepG2.2.15 cells. Northern blotting analysis confirmed that the expression of miR-181a, miR-181b, miR-200b and miR-146a were up-regulated and the expression of miR-15a was down-regulated, which was in consistent with the results of the microarray analysis. Furthermore, some putative miRNA targets were predicted and verified to be linked with mRNA expression. The 3′-UTR of HLA-A gene had one partially complementary site for miR-181a and miR-181 a might down-regulate the expression of HLA-A.Conclusion HBV replication modulates the expression of host cellular miRNAs, which may play a role in the pathogenesis of HBV-related liver diseases.

关键词： hepatitis B virus microRNA microarray gene regulation