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Agro-Morphological, Physico-Chemical and Molecular Characterization of Rice Germplasm with Similar Names of Bangladesh

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Rice science 2016年第4期23卷 211-218页

作者： mir sharf uddin ahmed Mohammad KHALEQUZZAMAN Md.Khairul BASHAR Abul Kalam Mohammad SHAMSuddin Genetic Resources and Seed Division Bangladesh Rice Research Institute HarvestP lus International Centre for Tropical Agriculture Department of Genetics and Plant Breeding Bangladesh Agricultural University

Thirty-one duplicate and similar named rice germplasms of Bangladesh were studied to assess the genetic variation for the agro-morphological and physico-chemical traits and simple sequence repeat banding patterns during 2009–2012 at Bangladesh Rice Research Institute. The range of variations within the cultivar groups showed higher degree. The principal component analysis showed that the first five components with vector values > 1 contributed 82.90% of the total variations. The cluster analysis grouped the genotypes into four clusters, where no duplicate germplasm was found. The highest number(11) of genotypes was constellated in cluster I and the lowest(3) in cluster II. The intra- and inter-cluster distances were the maximum in cluster I(0.93) and between clusters I and IV(24.61), respectively, and the minimum in cluster IV(0.62) and between clusters I and III(5.07), respectively. The cluster mean revealed that the crosses between the genotypes of cluster I with those of clusters II and IV would exhibit high heterosis for maximum good characters. A total of 350 alleles varied from 3(RM277) to 14(RM21) with an average of 7.8 per locus were detected at 45 microsatellite loci across the 31 rice accessions. The gene diversity ranged from 0.48 to 0.90 with an average of 0.77, and the polymorphism information content values from 0.44(RM133) to 0.89(RM206) with an average of 0.74. RM206, RM21, RM55, RM258 and RM433 were considered as the best markers on the basis of their higher polymorphism information content values. The dendrogram from unweighted pair-group method with arithmetic average clustering also classified the genotypes into four groups, where group IV comprised of 20 genotypes and group III of one genotype, but no duplicate was found. Finally, similar and duplicate named rice germplasms need to be conserved in gene bank as are distinct from each other.

关键词： diversity simple sequence repeat similar name landrace rice agro-morphological and physico-chemical trait

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Antibacterial efficacy of the seed extracts of Melia azedarach against some hospital isolated human pathogenic bacterial strains

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Asian Pacific Journal of Tropical Biomedicine 2011年第6期1卷 452-455页

作者： Abdul Viqar Khan Qamar uddin ahmed M Ramzan mir Indu Shukla Athar Ali Khan Department of Botany Faculty of Life SciencesAligarh Muslim University Department of Pharmaceutical Chemistry Facully of PharmacyInternational Islamic University Malaysia Department of Microbiology Faculty of Life SciencesAligarh Muslim University

Objective:To investigate the antibacterial potential of the polar and non-polar extracts of the seeds of Melia azedarach(***) L.(Meliaceae) against eighteen hospital isolated human pathogenic bacterial ***:Petrol,benzene,ethyl acetate,methanol,and aqueous extracts at five different concentrations(1,2,5,10 and 15 mg/mL) were *** diffusion method was followed to evaluate the antibacterial ***:All extracts of the seeds demonstrated significant antibacterial activity against tested *** all extracts,ethyl acetate extract revealed the highest inhibition *** present study also favored the traditional uses reported ***:Results of this study strongly confirm that the seed extracts of *** could be effective antibiotics,both in controlling gram-positive and gram-negative human pathogenic infections.

关键词： Melia azedarach Antibacterial efficacy Pathogenic bacteria

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Genetic polymorphisms of GSTM1,GSTP1 and GSTT1 genes and lung cancer susceptibility in the Bangladeshi population

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Asian Pacific Journal of Tropical Biomedicine 2014年第12期4卷 982-989页

作者： mir Muhammad Nasir uddin Maizbha uddin ahmed Mohammad Safiqul Islam Mohammad Siddiqul Islam Muhammad Shahdaat Bin Sayeed Yearul Kabir Abul Hasnat Department of Clinical Pharmacy and Pharmacology Faculty of PharmacyUniversity of Dhaka Department of Pharmacy Noakhali Science and Technology University Department of Biochemistry and Molecular Biology University of Dhaka

Objective:To verify possible associations between polymorphisms of glutathione S-transferase Mu(GSTM1),glutathione S-transferase θ(GSTT1) and glutathione S-transferase Pi(GSTP1)genes and susceptibility to lung ***:A total of 106 lung cancer patients and 116 controls were enrolled in a case-control *** GSTM1 and GSTT1 were analyzed using PCR while GSTP1 was analyzed using PCR-restriction fragment length *** of lung cancer was estimated as odds ratio at 95%confidence interval using unconditional logistic regression models adjusting for age,sex,and tobacco ***:GSTM1 null and GSTT1 null genotypes did not show a significant risk for developing lung cancer.A significandy elevated lung cancer risk was associated with GSTP1 heterozygous,mutant and combined heterozygous+mutant variants of *** classified by tobacco consumption status,no association with risk of lung cancer was found in case of tobacco smokers and nonsmokers carrying null and present genotypes of GSTM1 and GSTTL There is a three-fold(approximately) increase in the risk of lung cancer in case of both heterozygous(AG) and heterozygous+mutant homozygous(AG+GG) genotypes whereas there is an eightfold increase in risk of lung cancer in cases of GG with respect to AA genotype in ***:Carrying the GSTM1 and GSTT1 null genotype is not a risk factor for lung cancer and GSTP1Ile105 Val is associated with elevated risk of lung cancer.

关键词： Lung cancer Glutathione S-transferase Genetic polymorphism Smoking

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