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Evaluation of a Rapid Diagnostic Test, Boson Biotech SARS CoV-2 Ag, for the Detection of SARS-CoV-2 in Gabon

Advances in Infectious Diseases 2024年第2期14卷 469-477页

作者： Samira Zoa Assoumou Ulrich Leger Davy Mouangala Ludovic Mewono Davy-Christ Angoune Ndong Guy Paterne Malonga Mbembo Nely Meungang Alain Moutsinga Elvyre Anita Mbongo Kama rodrigue mintsa nguema Dpartement de Bactriologie-Virologie Universit des Sciences de la Sant Libreville Gabon Laboratoire Professeur Daniel Gahouma Libreville Gabon Departement des Sciences de la vie et de la Terre Ecole Normale Suprieure Libreville Gabon Centre de sant de Glass Libreville Gabon Laboratoire National de Sant Publique Libreville Gabon Dpartement de Biologie et Ecologie Animale Centre National de la Recherche Scientifique et Technologique Libreville Gabon Laboratoire dEcologie des Maladies Transmissibles (LEMAT) Universit Libreville Nord (ULN) Libreville Gabon

1) Background: Rapid and acurate diagnostic testing for case identification, quarantine, and contact tracing is essential for managing the COVID 19 pandemic. Rapid antigen detection tests are available, however, it is important to evaluate their performances before use. We tested a rapid antigen detection of SARS-CoV-2, based on the immunochromatography (Boson Biotech SARS-CoV-2 Ag Test (Xiamen Boson Biotech Co., Ltd., China)) and the results were compared with the real time reverse transcriptase-Polymerase chain reaction (RT-PCR) (Gold standard) results;2) Methods: From November 2021 to December 2021, samples were collected from symptomatic patients and asymptomatic individuals referred for testing in a hospital during the second pandemic wave in Gabon. All these participants attending “CTA Angondjé”, a field hospital set up as part of the management of COVID-19 in Gabon. Two nasopharyngeal swabs were collected in all the patients, one for Ag test and the other for RT-PCR;3) Results: A total of 300 samples were collected from 189 symptomatic and 111 asymptomatic individuals. The sensitivity and specificity of the antigen test were 82.5% [95%CI 73.8 - 89.3] and 97.9 % [95%CI 92.2 - 98.2] respectively, and the diagnostic accuracy was 84.4% (95% CI: 79.8 - 88.3%). The antigen test was more likely to be positive for samples with RT-PCR Ct values ≤ 32, with a sensitivity of 89.8%;4) Conclusions: The Boson Biotech SARS-CoV-2 Ag Test has good sensitivity and can detect SARS-CoV-2 infection, especially among symptomatic individuals with low viral load. This test could be incorporated into efficient testing algorithms as an alternative to PCR to decrease diagnostic delays and curb viral transmission.

关键词： SARS-CoV-2 Rapid Diagnostic Test Evaluation COVID-19 Antigen Performance

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Apparent Densities of Stomoxys Species (Diptera, Muscidae) of Different Physiological Ages Caught with Vavoua Trap Differ With Landscape and Trapping Period

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Journal of Zoological Research 2021年第1期3卷 9-14页

作者： Sevidzem S.Lendzele Koumba A.Aubin Zinga-Koumba C.Roland mintsa-nguema rodrigue Jacques F.Mavoungou Ecole Doctorale des Grandes Ecoles(EDGE) LibrevilleGabon Laboratoire d’Ecologie Vectorielle(LEV-IRET) LibrevilleGabon Institut de Recherche en Ecologie Tropicale(IRET) BP 13354LibrevilleGabon Universitédes Sciences et Techniques de MASUKU BP 941FrancevilleGabon

Stable flies(Stomoxys spp.)are common pests of livestock in the peri-urban rangelands of Vina Division of the Adamawa *** documented information is available on their diurnal dynamics in relation to physiological age and *** main aim of this study was to determine the trap apparent density(ADT)of Stomoxys and associate it to their diurnal activity rhythm and *** traps(n=12)were used for Stomoxys *** was carried out for seven days *** days consecutively in August and four days consecutively in October,*** traps were pitched in each of the three biotopes(river,cattle corral and gallery forest)of the four study sites(Galim,Mbidjoro,Velambai and Soukourwo).All female Stomoxys captured underwent ovarian dissection for the determination of their physiological ages(parous or nulliparous).A total of 218 Stomoxys were caught and identified into four species(*** niger,***,***,and ***)with an overall ADT of 2.59 flies per trap per day and Stomoxys niger niger(1.13 *** trap per day)recorded the highest *** richness was study site *** catches of females 113(51.83%)were made than that of their male 105(48.17%)*** recorded the highest Stomoxys apparent density(4.90)as compared to other sites with a statistically significant difference(P<0.05).The proportion of parous 87(59.30%)was higher than that of their nulliparous 46(40.71%)*** differed with landscape and capture *** was the most frequent in all biotopes and Galim recorded the highest *** population of female flies was made up of adults(parous)with a bimodal diurnal activity rhythm whereas males had a unimodal activity rhythm.

关键词： Abundance Parity Landscape Trapping period Stomoxys Vina Cameroon

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Use of the “Veda-Lab Easy Reader+” for the Determination of T3, T4 and TSH Hormones in the Mountainous Population of Benin: Case of Natitingou

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Advances in Bioscience and Biotechnology 2024年第1期15卷 39-51页

作者： Pamphile Thierry Houngbo Natacha Aivodji rodrigue Akotegnon Alphonse Sezan Laboratory of Biomembranes and Cellular Signaling Department of Animal Physiology University of Abomey-Calavi Cotonou Benin

The thyroid, an endocrine gland located at the base of the neck, produces thyroid hormones (triiodothyronine (T3) and thyroxine (T4)). The production of these hormones is possible by iodine and other nutrients such as selenium and certain vitamins. To assess the thyroid disturbances in the mountain population of Benin, a survey was conducted in Natitingou, a mountain town located in the department of Atacora, in the northwest of Benin, on a sample of thirty (30) adults (15 men and 15 women), most of whom are educated. The results of the questionnaire revealed that 43% of the surveyed population acknowledged having knowledge on the mentioned subject and have dietary habits based on the consumption of seafood, and also legumes (20%). The examination of the serum results of the dosage of T3, T4 and TSH hormones revealed cases of thyroid disturbances in the region (36.32% in men and 44.98% in women). The analysis of a comparative table including the “VEDALAB Easy Reader+” and five (05) other readers, presents the performance, reading techniques, principles, advantages and disadvantages of each device. Pending further studies, some recommendations were made at the end of this study to the academic authorities regarding probable cases of dysthyroidism for which additional examinations are required and an awareness for the improvement of dietary habits.

关键词： Thyroid TSH T3 T4 “VEDALAB Easy Reader+”

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Evaluation of a Rapid Diagnostic Test, Boson Biotech SARS CoV-2 Ag,for the Detection of SARS-CoV-2 in Gabon

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Advances in Infectious Diseases（传染病进展） 2024年第2期 469页

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Microstrip Leaky-Wave Antenna for Radar Applications

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Open Journal of Antennas and Propagation 2024年第2期12卷 19-29页

作者： Ronelle Rudaï Gochincka Gogom Charmolavy Goslavy Lionel Nkouka Moukengue Brice rodrigue Malonda-Boungou Electrical and Electronic Engineering Laboratory ENSP Marien Ngouabi University Brazzaville Congo Groupe de Simulations Numriques en Magntisme et Catalyse Facult des Sciences et Techniques Universit Marien Ngouabi Brazzaville Congo Institut National de Recherches en Sciences Exactes et Naturelles (IRSEN) Brazzaville Congo Facult des Sciences Appliques Universit Denis Sassou-Nguesso Kintl Congo

The work in this article focuses on developing and improving the performance of new leaky-wave antenna configurations that can be adapted for use in radar systems. The study focused on the W-band, where we demonstrated the possibility of modifying resonant frequencies and reducing the number of patches required. The antenna was designed using HFSS, based on the finite element method. It we designed enabled us to observe the influence of the number of patches on the radiation pattern, and also to achieve low levels of minor’s lobes. and good directivity at the operating frequency. These patches are arranged in the shape of an inverted T. The interest of this study is to meet the requirements of radar antennas dedicated to detection.

关键词： Leaky-Wave Antenna Microstrip HFSS Radar Applications

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Characterization of Aquifers in Crystalline and Crystallophyll Basement Zones Using the Electrical Resistivity Method (Trails and Electrical Soundings) in the Gagnoa Region, (Central-Western Côte d’Ivoire)

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International Journal of Geosciences 2024年第6期15卷 511-523页

作者： Oscar Zahibo Onétié Assoué Kouakou Sylvestre Kouadio Kotchi rodrigue Orou Maxime Assa Abe Department of Geosciences Peleforo Gon Coulibaly University Korhogo Cte dIvoire Department of Agriculture and New Technologies University of San Pedro San Pedro Cte dIvoire

Introduction: Located in the central-western part of Côte d’Ivoire, the subsoil of the Gagnoa region is made up of sedimentary volcano formations and granitoids with developed fracturing. This complex Precambrian basement contains most of the region’s water resources. This is at the origin of the high failure rate during the various hydrogeological prospecting campaigns. Methodology: The database consists of resistivities from 42 holes and 51 trails drilled as part of the implementation of high-throughput drilling in the study area. The objective of this study is to deepen the knowledge of the fissured basement by interpreting profile curves and electrical soundings. It will be a question of classifying the different types of anomalies obtained on the profiles and their shapes. The orientation of the lineaments observed on the profiles was determined. Results: The interpretation of the geophysical data revealed various anomalies, the main ones being of the CC (Conductor Compartment) and CEDP (Contact between two bearings) types. These types of anomalies are mainly expressed in various forms: the “V”, “W” and “U” shapes. From these anomalies and the appearance of the electrical profiles, lineaments and their orientations were identified with N90-100, N130-140, N170-180 as major orientations. Conclusion: These results could contribute to a better understanding of the fractured environment of the Gagnoa region.

关键词： Basement Electrical Profiles Sounding Curves Resistivities

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Papillary Carcinoma on Stroma Ovarii: A Case Report from Madagascar and Review of the Literature

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Journal of Cancer Therapy 2024年第3期15卷 57-61页

作者： Toky Rakotoarivo Christiana Ranaivoson Vonjy Andrianarison Nomeharisoa rodrigue Emile Hasiniatsy Florine Rafaramino Department of Oncology University Hospital Tanambao Antsiranana Madagascar Department of Oncology University Hospital Joseph Ravoahangy Andrianavalona Antananarivo Madagascar Department of Radiotherapy University Hospital Joseph Ravoahangy Andrianavalona Antananarivo Madagascar Department of Oncology and Pallliative Care Soavinandriana Hospital Center Antananarivo Madagascar

A 64-year-old woman with no particular history presented with chronic pelvic pain since November 2021. The ultrasound performed showed multiple right ovarian cystic ranging from 0.4 to 4 cm on the long axis. She underwent a right salpingo-oophorectomy in May 2022. The anatomical pathology result is in favor of a papillary carcinoma developed on stroma ovarii. No adjuvant treatment was necessary since the tumor was well limited, without capsular rupture, stage IA. The thyroid test was normal. The patient is currently being monitored. There is no sign of recurrence 20 months after surgery.

关键词： Papillary Carcinoma Surgery Struma Ovarii

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Characterisation of Human-Wildlife Conflicts around the Kahuzi-Biega National Park, Eastern Democratic Republic of Congo

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Journal of Environmental Protection 2024年第3期15卷 343-356页

作者： Basubi Muke Matthieu Vwima Stany Ayagirwe Basengere rodrigue Mushagalusa Freddy Benjamin-Fink Nicole Bobo Kadiri Serge Faculty of Agricultural and Environmental Sciences Evangelical University in Africa Bukavu Congo Wildlife and Protected Areas Laboratory Forestry and Wood Technology Department of Forestry University of Dschang Dschang Cameroon Faculty of Economics and Management Sciences Evangelical University in Africa Bukavu Congo Conservation beyond Borders Organization Minnetonka USA

The history of mankind contains so many illustrations of well-classified and violent struggles against various animals over crop production in agricultural fields, attacks on human life or competition in order to have access to certain natural resources. The aim of this study is to characterise the human-wildlife conflicts that occur around the KBNP, especially with regard to primates. Surveys were carried out in 260 households selected using the snowball method. The results showed that human-wildlife conflicts around the KBNP are characterised by the destruction of crops in riparian fields by monkeys, chimpanzees and gorillas, the destruction of houses, physical attacks and zoonoses. Despite the conservation and protection measures for wildlife and local populations put in place by the Park’s managers, conflicts between local populations and the Park’s wild animals are still visible. These conflicts lead to the detention of wild animals by local people roaming in the villages and to poaching on the Park’s boundaries. The weakness of the community management policy on the part of the park managers and the resentment of the indigenous peoples towards the restriction of their access to natural resources are the major constraints on the sustainable management of conflicts between the local populations and the wild animals in the KBNP. The strategies for the sustainable resolution of human-wildlife conflicts around the KBNP must be geared towards and integrated into a community conservation approach.

关键词： Community Conservation Human-Wildlife Conflict Kahuzi-Biega National Park

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Granulometry Assessment of Lom Pangar Dam Sediments (East-Cameroon)

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Journal of Geoscience and Environment Protection 2024年第6期12卷 207-231页

作者： rodrigue Fotie Lele Mihaela Amalia Diminescu Issoufou Ouedraogo Annette Madelene Dăncila Souleymane Pelede Alphonse Emadak Martin Lompo Department of Geology University Joseph Ki Zerbo Ouagadougou Burkina Faso Department of Water and Energy Green Heart International Bafoussam Cameroon Department of Hydraulic Machineries and Environmental Engineering National University of Science and Technology Politehnica Bucharest Bucharest Romania Department of Mining Engineering University Yembila-Abdoulaye-Toguyeni Fada NGourma Burkina Faso Department of Inorganic Chemistry University of Yaound I Yaound Cameroon

The Lom Pangar dam, the largest reservoir in Cameroon with a storage capacity of 6 km3 and a 30 MW hydropower plant, primarily regulates the hydrologic regime of the Sanaga River to maintain hydropower efficiency during dry seasons and enhance downstream hydropower plant performance. Understanding and managing sediments are crucial for the sustainability of dams, as indicated by numerous studies. This study assessed the granulometry of the sediments transported across the reservoir. For that purpose, 6 samples of fresh sediments were collected in the lacustrine and transitional sections of the reservoir using the standard method. Particles size was assessed using the laser diffusion technic after a 3 mm sieving. Various granulometric parameters were derived from the literature to analyze and characterize those sediments. Results show that silts are more than 70% of particles size and range between 2.19 - 60.26 µm. Size distribution also shows the same trend with D75 less than 51 µm. This is confirmed by the low values of Inman Skewness SkΦ (−0.168 to 0.303). The Sorting index S0 ranges from 0.31 to 0.53 µm, showing a very well-sorted sediments, aligning with low values of Krumbein index (0.906 - 1.683) that express the low heterometry of the particles. The consequence on the dam will be a quick clogging of the bottom of the reservoir. Their pH varies from 7.0 to 7.5. It also appears that the sandy fraction trend is higher in the right bank of the dam and reaches 22% on the right bank of Pangar River due to crystalline geology. Fraction greater than 3000 µm is negligible. The management of the dam has to keep attention to these results as siltation may close the safety outlet of the dam, damage turbines, and provoke recurrent technical and safety issues. Further, the clogging of the bottom of the reservoir may lead to an ecological problem with the limitation of hyporheic flow. Thus, water exchange with the underground water table and the natural purification of wate

关键词： Lom Pangar Dam Sediments Granulometry Sustainability Hydropower Dam Safety

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Eudragit^®-PEG Nanoparticles: Physicochemical Characterization and Interfacial Tension Measurements

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Open Journal of Biophysics 2024年第2期14卷 121-131页

作者： Papa Mady Sy Sidy Mouhamed Dieng Alphonse rodrigue Djiboune Louis Augustin Diaga Diouf Boucar Ndong Gora Mbaye Mounibé Diarra Laboratory of Physics and Pharmaceutical Biophysics Faculty of Medicine Pharmacy and Odontology UCAD Dakar Senegal Laboratory of Galenic Pharmacy Faculty of Medicine Pharmacy and Odontology UCAD Dakar Senegal Laboratory of Medical Biophysics and Nuclear Medicine Faculty of Medicine Pharmacy and Odontology UCAD Dakar Senegal

The objectives of this study are to understand the mechanisms involved in the stabilization of water/oil interfaces by polymeric nanoparticles (NPs) (Eudragit®). Eudragit L100 NPs of various sizes and Zeta potentials were studied and compared at a water/cyclohexane model interface using a droplet tensiometer (Tracker Teclis, Longessaigne, France). The progressive interfacial adsorption of the NPs in the aqueous phase was monitored by tensiometry. The model interface was maintained and observed in a drop tensiometer, analyzed via axisymmetric drop shape analysis (ADSA), to determine the interfacial properties. Given the direct relationship between the stability of Pickering emulsions (emulsions stabilized by solid nanoparticles) and the interfacial properties of these layers, different nanoparticle systems were compared. Specifically, Eudragit NPs of different sizes were examined. Moreover, the reduction of the Zeta potential with PEG-6000 induces partial aggregation of the NPs (referred to as NP flocs), significantly impacting the stability of the interfacial layer. Dynamic surface tension measurements indicate a significant decrease in interfacial tension with Eudragit® nanoparticles (NPs). This reduction correlates with the size of the NPs, highlighting that this parameter does not operate in isolation. Other factors, such as the contact angle and wettability of the nanoparticles, also play a critical role. Notably, larger NPs further diminished the interfacial tension. This study enhances our understanding of the stability of Pickering emulsions stabilized by Eudragit® L100 polymeric nanoparticles.

关键词： Nanoparticles Eudragit® PEG Interfacial Tension Pickering Emulsion

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