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Differences in the Histopathology and Cytokine Expression Pattern between Chronological Aging and Photoaging of Hairless Mice Skin

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Modern Research in Inflammation 2014年第3期3卷 82-89页

作者： Masaaki Sakura Yoichi Chiba Emi Kamiya Ayako Furukawa Noriko Kawamura Masanao Niwa minoru takeuchi Yasushi Enokido Masanori Hosokawa Department of Pathology Institute for Developmental Research Aichi Human Service Center Kasugai Japan Faculty of Life Science Kyoto Sangyo University Kyoto Japan Faculty of Pharmaceutical Sciences Suzuka University of Medical Science Suzuka Japan Fundamental Research Laboratory General Research & Development Institute Hoyu Co. Ltd. Nagakute Japan

Skin photoaging is a complex, multifactorial process resulting in functional and structural changes of the skin, and different phenotypes from chronological skin aging are well-recognized. Ultraviolet (UV)-irradiated hairless mice have been used as a skin photoaging animal model. However, differences in morphology and gene expression patterns between UV-induced and chronological skin changes in this mouse model have not been fully elucidated. Here we investigated differences in histopathology and cytokine expression between UV-irradiated and non-irradiated aged hairless mice to clarify the factor(s) that differentiate photoaging from chronological skin aging phenotypes. Eight-week-old HR-1 hairless mice were divided into UV-irradiated (UV-irradiated mice) and non-irradiated (control mice) groups. Irradiation was performed three times per week for 10 weeks. In addition, 30-week-old HR-1 hairless mice were reared until 70 weeks of age without UV irradiation (aged mice). Histopathologies revealed that the flattening of dermal-epidermal junctions and epidermal thickening were observed only in UV-irradiated mice. Decreases in fine elastic fibers just beneath the epidermis, the thickening of elastic fibers in the reticular dermis, and the accumulation of glycosaminoglycans were more prominent in UV-irradiated mice as compared to non-irradiated aged mice. Quantitative PCR analyses revealed that UV-irradiated mice showed an increase in the expression of IFN-γ. In contrast, aged mice exhibited proportional up-regulation of both pro-inflammatory and anti-inflammatory cytokines. The IFN-γ/IL-4 ratio, an indicator for the balance of pro-inflammatory and anti-inflammatory cytokines, was significantly higher in UV-irradiated mice as compared to control and non-irradiated aged mice. An elevated IFN-γ/IL-4 ratio was also observed in aged senescence-accelerated mouse-prone 1 (SAMP1) mice, a spontaneous skin photoaging model we recently reported. Thus, an imbalance between pro-inflam

关键词： Photoaged Skin Chronologically-Aged Skin Pro-Inflammatory Cytokines Anti-Inflammatory Cytokines Hairless Mice

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Effect of Hot Water Extract from Agaricus Blazei Murill on Chemotaxis of Neutrophils

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Journal of Cosmetics, Dermatological Sciences and Applications 2013年第1期3卷 12-17页

作者： Mayuko Miyagawa Yuriko Hirono Ayaka Kawazoe Eri Shigeyoshi Masahito Nose Masaaki Sakura K. E. Pinkerton minoru takeuchi Center for Health and the Environment University of California Davis USA. Department of Animal Medical Science Faculty of Life Sciences Kyoto Sangyo University Kyoto Japan

Hot water extract from the edible Brazilian mushroom, Agaricus Blazei Murill (ABM), is used for both traditional and alternative medicine. ABM is reported to stimulate anti-tumor, anti-infection, and immune activity. However, there are few reports of how ABM affects neutrophils. Therefore, in this study, we examined the effect of hot water ABM extract on neutrophil migration, phagocytosis, and reactive oxygen species production using neutrophils from guinea pig. Migratory direction and velocity as indicators of chemotactic activity of neutrophils were significantly (p 0.001) increased at concentration of 50 and 100 mg/ml in ABM extract compared with control. Phagocytic activity of neutrophil was significantly (p 0.01) increased at concentration of 5 mg/ml in ABM extract compared with control. Production of reactive oxygen species (ROS: H2O2 or ) by neutrophils was significantly (p 0.01) increased at concentration of 5 mg/ml in ABM extract compared with control. These results suggest that enhancement in neutrophil chemotactic activity, phagocytic activity and ROS production are mechanisms by which ABM extract inhibits bacterial infection in the skin and dermatitis.

关键词： Neutrophil Chemotactic Activity Phagocytic Activity ROS Production Agaricus Blazei Murill

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Alveolar macrophage functions and DNA damage in cigarette smoke-exposed mice

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Advances in Bioscience and Biotechnology 2013年第8期4卷 1-7页

作者： Yuriko Hirono Yasuyuki Tanahashi Kazuma Sasaki Kenjiro Konno Yuki Shirai Kengo Kobayashi Azusa Someya Sumire Inaga Masaaki Sakura Kent E. Pinkerton minoru takeuchi Center for Health and the Environment University of California Davis Davis USA Department of Animal Medical Science Faculty of Life Sciences Kyoto Sangyo University Kyoto Japan Division of Genome Morphology Department of Functional Morphological and Regulatory Science Faculty of Medicine Tottori University Yonago Japan

Alveolar macrophages (AM) are known to play an essential role in lung defense through their ability to remove the foreign matters reaching the lung alveoli. Cigarette smoke (CS) is a critical risk factor for many lung diseases. CS is inhaled into the lung by respiretion and affects AM. It has been previously reported that CS induces inhibition of cytokine production, cell surface receptor expression and antigen presentation in AM. However, the relationship of immune suppression and DNA damage caused by CS in AM is still unclear. Therefore, in this study, we investigated AM immune function and DNA damage in CS-exposed mice. Mice were exposed to CS of 20 cigarettes/day during 10 days using a HambrugⅡsmoking machine. After exposure, AM were obtained by bronchoalveolar lavage. The number of AM was significantly increased in CS-exposed mice compared with non-CS-exposed mice. Phagocytic activity of AM was significantly inhibited by CS exposure. Percentage of CD11b-, CD14-, Toll-like receptor (TLR)2- or TLR4-positive cells was significantly decreased in CS-exposed mice compared with non-CS-exposed mice. Interleukin-1β mRNA expression in lipopolysaccharide-stimulated AM was significantly inhibited by CS exposure. Intracellular reactive oxygen species (ROS) (, H2O2) production of AM was significantly increased, and DNA damage was induced by CS exposure. These results suggest that impaired immune functions by CS exposure may be related to DNA damage via excessive ROS induced by CS. These alterations of AM caused by CS could be associated with infection and development of pulmonary diseases.

关键词： Alveolar Macrophages Phagocytic Activity ROS Production DNA Damage Cigarette Smoke

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Pre-hepatectomy type Ⅳ collagen 7S predicts post-hepatectomy liver failure and recovery

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World Journal of Gastroenterology 2020年第7期26卷 725-739页

作者： Masatsugu Ishii Osamu Itano Masahiro Shinoda minoru Kitago Yuta Abe Taizo Hibi Hiroshi Yagi Ayano takeuchi Hanako Tsujikawa Tokiya Abe Yuko Kitagawa Department of Surgery Keio University School of MedicineTokyo 160-8582Japan Department of Hepato-Biliary-Pancreatic and Gastrointestinal Surgery International University of Health and Welfare School of MedicineChiba 286-8686Japan Department of Pediatric Surgery and Transplantation Kumamoto UniversityKumamoto 860-8556Japan Department of Public Health Keio University School of MedicineTokyo 160-8582Japan Department of Pathology Keio University School of MedicineTokyo 160-8582Japan

BACKGROUND Liver resection is an effective treatment for benign and malignant liver ***,a method for preoperative evaluation of hepatic reserve has not yet been *** reported assessments of preoperative hepatic reserve focused only on liver failure in the early postoperative period and did not consider the long-term recovery of hepatic *** determining eligibility for hepatectomy,the underlying pathophysiology needs to be considered to determine if the functional hepatic reserve can withstand both surgery and any postoperative *** To identify pre-hepatectomy factors associated with both early postoperative liver failure and long-term postoperative liver function *** This study was a retrospective cohort *** retrospectively investigated 215 patients who underwent hepatectomy at our hospital between May 2013 and December *** post-hepatectomy liver failure(PHLF)was defined using the International Study Group of Liver Surgery’s definition of ***-term postoperative recovery of liver function was defined as the time taken for serum total bilirubin and albumin levels to return to levels of2.8 g/dL,respectively,and the time taken for Child-Pugh score to return to Child-Pugh class *** Preoperative type IV collagen 7S was identified as a significant independent factor associated with both PHLF and postoperative long-term recovery of liver *** analysis revealed that the time taken for the recovery of Child-Pugh scores and serum total bilirubin and albumin levels was significantly shorter in patients with type IV collagen 7S≤6 ng/mL than in those with type IV collagen 7S>6 ng/*** additional analyses,similar results were observed in patients without chronic viral hepatitis associated with *** Preoperative type IV collagen 7S is a preoperative predictor of PHLF and longterm postoperative liver function *** can also be used in patients without chron

关键词： Hepatectomy Liver failure Type Ⅳ collagen 7S Liver fibrosis Postoperative complications Long-term postoperative liver function recovery

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Arterial pulsation on a human patient simulator improved students’ pulse assessment

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Journal of Biomedical Science and Engineering 2012年第5期5卷 285-289页

作者： Akihiro takeuchi Tomomi Kobayashi minoru Hirose Takashi Masuda Toshiro Sato Noriaki Ikeda Department of Medical Informatics School of Allied Health Sciences Kitasato University Sagamihara Japan Department of Medical Safety Engineering School of Allied Health Sciences Kitasato University Sagamihara Japan Department of Rehabilitation School of Allied Health Sciences Kitasato University Sagamihara Japan

Even with basic cardiovascular lectures, undergraduates do not usually experience the reality of palpation and, therefore, cannot integrate their physiological knowledge. We created a pulse training scenario of human patient simulators (HPS) to recognize and assess the normal and arrhythmic pulse of the radial artery. All 25 participants were recruited as volunteers to the study from the School of Allied Health Sciences, Kitasato University. Participants received training in radial palpation of arrhythmias on HPS. The test scenario included 10 arrhythmic pulses combined with normal pulses and weak pulses. The average examination scores significantly improved, from 23.8 ± 2.8 of the pretest to 72.9 ± 3.4 of the posttest (mean and SE, N = 25, p < 0.00001). A questionnaire and general written comments for the palpation training were positive. The palpation training improved the participants’ assessment of radial pulses.

关键词： Education Physiology Human Patient Simulator Arrhythmic Pulse Pulse Assessment

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Low-acoustic attenuation and high-mechanical strength silicone rubber lens doped with ZnO nano-powder for medical array probe

Low-acoustic attenuation and high-mechanical strength silico...

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2008 IEEE International Ultrasonics Symposium

作者： Yohachi(John)Yamashita Yasuharu Hosono Noriko Yamamoto Kazuhiro Itsumi Yasuhisa Makita Takashi takeuchi Koichi Shibamoto minoru Aoki Hiroyuki Shikata Toshiba Research Consulting Corp. KawasakiKanagawa Corporate R &D Center.Toshiba Corp.Kawasaki Kanagawa Toshiba Medical Systems Corp. OtawaraTochigi

＜正＞Background,Motivation and Objective Silicone（Q）rubber has been the predominant externally focused acoustic lens material applied for the medical array probe application since 1970,because it is safe,has a suit...

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