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马脐静脉内皮细胞分离培养及其功能验证

中国畜牧兽医 2024年第7期51卷 2953-2962页

作者：赵比力格温鑫伊敏娜才文道力玛神英超斯琴照伦其其格任宏芒来格日乐其木格内蒙古农业大学动物科学学院内蒙古自治区马属动物科学研究与技术创新重点实验室呼和浩特010020

【目的】建立一种简便且高效的体外马脐静脉内皮细胞(equine umbilical vein endothelial cells,eUVECs)分离和培养方法,并提供一个可靠的细胞模型,用于研究马的血管生成功能。【方法】在无菌条件下,从马脐带中分离静脉,并用PBS液彻底... 详细信息

【目的】建立一种简便且高效的体外马脐静脉内皮细胞(equine umbilical vein endothelial cells,eUVECs)分离和培养方法,并提供一个可靠的细胞模型,用于研究马的血管生成功能。【方法】在无菌条件下,从马脐带中分离静脉,并用PBS液彻底冲洗。使用0.2%Ⅰ型胶原酶对脐静脉进行消化25~30 min后,收集分离的eUVECs进行培养。每12 h使用倒置显微镜观察eUVECs的生长状况。使用CCK-8法测定第1代(P1)和P3代eUVECs在7 d内的D450 nm值,并绘制生长曲线。通过免疫荧光染色方法鉴定内皮细胞标记物CD31和CD34的表达情况。使用间充质干细胞成脂和成骨分化诱导试剂诱导eUVECs,以验证其是否具有分化特性。确定内皮细胞后,添加梯度上升浓度(5、10、15、20、25 ng/mL)的肿瘤坏死因子-α(TNF-α)刺激eUVECs,使用CCK-8法检测细胞活性,观察细胞形态变化。使用Matrigel法培养eUVECs,用ImageJ软件分析数据,并统计新生血管的4个指标:成管交叉点、成管长度、成管数和成管面积。寻找体外血管生成能力的最佳TNF-α刺激浓度。【结果】使用0.2%Ⅰ型胶原酶消化法分离的eUVECs 4~5 d内的汇合度达到90%。在倒置显微镜下观察,这些细胞生长良好并呈铺路石状排列。通过免疫荧光染色确认了CD31和CD34在eUVECs中的阳性表达;并且eUVECs表现出薄弱的成脂和成骨分化能力。在Matrigel法培养条件下,20 ng/mL TNF-α处理组的成管交叉点数、成管长度、成管数和成管面积等血管形成能力指标最高,且极显著高于对照组(P<0.01)。然而,在常规培养条件下,随着TNF-α浓度增加到15 ng/mL,eUVECs的增殖受到显著抑制甚至开始导致细胞的凋亡。【结论】Ⅰ型胶原酶脐带注满消化法能够成功分离培养出原代eUVECs,20 ng/mL TNF-α显著增强eUVECs体外血管生成能力,eUVECs可以作为体外研究血管生成的细胞模型。

关键词：马脐静脉内皮细胞 Ⅰ型胶原酶消化法培养鉴定 TNF-α 血管新生

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公立医院集团化管理背景下的病案编码中心建设实践

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中华医院管理杂志 2024年第2期40卷 102-106页

作者：吴丽娟郭佳奕徐敏慧金旎葛邦彪郑盼何国斌郭敏娜浙江大学医学院附属金华医院质量控制办公室金华321000

病案首页数据质量是保障疾病诊断相关分组(DRG)付费和公立医院绩效考核顺利实施的重要基础。2021年1月, 某公立医院医疗集团组建病案编码中心统一管理成员单位的病案首页编码。通过建章立制、信息系统构建、人力资源整合和配置优化、编... 详细信息

病案首页数据质量是保障疾病诊断相关分组(DRG)付费和公立医院绩效考核顺利实施的重要基础。2021年1月, 某公立医院医疗集团组建病案编码中心统一管理成员单位的病案首页编码。通过建章立制、信息系统构建、人力资源整合和配置优化、编码人员能力建设、建立与临床科室的协作机制等措施, 实现了病案首页集团同质化管理, 提高了病案首页质量。临床医生的病案首页填写错误率从2020年的11.15%下降至2022年的7.98%, 编码员的编码错误率从2020年的3.45%下降至2021年2.39%, 可为各医疗机构的病案首页质量管理和医院精细化管理提供参考。

关键词：医院公立集团化管理病案管理国际疾病分类疾病诊断相关分组同质化管理质量控制

基于OBE理念的推进剂专业工程类课程教学实践探究——以化学工程基础课程为例

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大学教育 2024年第1期 46-48,55页

作者：高敏娜黄智勇金国锋贾瑛吕晓猛中国人民解放军火箭军工程大学陕西西安710025

针对推进剂专业工程类课程教学目前存在的教学资源匮乏、教学方法单一、理论与实践脱离、思政育人不够深入等问题,课题组以推进剂专业育人目标为指导,在丰富课程资源体系、强化师资队伍、改革教学方法、理论与实践结合、加强思政育人等... 详细信息

针对推进剂专业工程类课程教学目前存在的教学资源匮乏、教学方法单一、理论与实践脱离、思政育人不够深入等问题,课题组以推进剂专业育人目标为指导,在丰富课程资源体系、强化师资队伍、改革教学方法、理论与实践结合、加强思政育人等方面进行改革和创新,有效提升了学生的学习兴趣、课堂参与度及实践能力,提高了学生分析问题、解决问题的能力。

关键词： OBE理念推进剂专业特色改革创新

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光催化氧化绿色合成医用化学发光试剂

大学化学 2024年第5期39卷 134-143页

作者：马敏娜欧阳玉瑾邬源袁明伟杨丽娟云南民族大学化学与环境学院生物基材料绿色制备技术国家地方联合工程中心昆明650500

光催化氧化较传统氧化反应更具有安全可控、条件温和、绿色环保的优点,已成为化学前沿中的研究热点之一,并受到广泛关注。目前,受实验条件和设备的限制,光催化氧化反应在本科教学实验中较为缺乏。本实验利用实验室常规仪器组装出简易光... 详细信息

光催化氧化较传统氧化反应更具有安全可控、条件温和、绿色环保的优点,已成为化学前沿中的研究热点之一,并受到广泛关注。目前,受实验条件和设备的限制,光催化氧化反应在本科教学实验中较为缺乏。本实验利用实验室常规仪器组装出简易光反应装置,以高效低能的钠灯为光源催化氧化磷酸盐中间体,实现了1,2-二氧环乙烷类医用化学发光试剂的实验室微量化制备,丰富了光化学反应在实验教学中的应用。本实验使用的原料和试剂便宜易得,操作简便,产物收率高,实验既可实现单瓶独立操作,又可进行多瓶串联反应。本文综合考虑实验成本、实验与教学效率,以两瓶串联方式展开分组实验教学,在实现教学形式多样化的同时,培养学生合作意识,适合本科实验教学,帮助学生提高全面综合的实验操作能力,加深对自由基加成等反应机理的理解。

关键词：光催化氧化安全可控医用化学发光试剂自由基加成

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Economic Evaluation of the Comprehensive AIDS Prevention and Control Program—Tianjin Municipality,China,2011-2022

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China CDC weekly 2023年第48期5卷 1067-1072页

作者： Maohe Yu Jianping Wang Jinhua Zhao Yan Guo Jianyun Bai Zhongquan Liu Hui Gong Tielin Ning minna Zheng Jinyu Hou Zhuang Cui STD&AIDS Control and Prevention Section Tianjin Centers for Disease Control and PreventionTianjinChina Tianjin Key Laboratory of Pathogenic Microbiology of Infectious Disease Tianjin Centers for Disease Control and PreventionTianjinChina Department of Epidemiology and Biostatistics School of Public HealthTianjin Medical UniversityTianjinChina Tianjin Key Laboratory of Environment Nutrition and Public HealthTianjinChina

What is already known about this topic?Acquired immunodeficiency syndrome(AIDS)represents a significant public health challenge globally,not only inflicting harm on the health of individuals but also placing a considerable economic strain on *** is added by this report?This study represents the inaugural report on the potential reduction in economic burden attributable to human immunodeficiency virus(HIV)prevention strategies in *** 2011 and 2022,it is estimated that effective measures could prevent 2,965 new HIV infections and avert 658 deaths,resulting in an economic benefit of approximately 14.437 billion Chinese *** are the implications for public health practice?The findings of this study offer valuable evidence to inform the development of localized HIV prevention and control strategies,as well as to guide public health policymaking.

关键词： prevention globally Tianjin

Diet and epigenetics in colon cancer

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World Journal of Gastroenterology 2009年第3期15卷 257-263页

作者： minna Nystrm Marja Mutanen Department of Biological and Environmental Sciences GeneticsUniversity of HelsinkiFI-00014 HelsinkiFinland Department of Applied Chemistry and Microbiology Division of NutritionUniversity of HelsinkiFI-00014 HelsinkiFinland

Over the past few years, evidence has accumulated indicating that apart from genetic alterations, epigenetic alterations, through e.g. aberrant promoter methylation, play a major role in the initiation and progression of colorectal cancer (CRC). Even in the hereditary colon cancer syndromes, in which the susceptibility is inherited dominantly, cancer develops only as the result of the progressive accumulation of genetic and epigenetic alterations. Diet can both prevent and induce colon carcinogenesis, for instance, through epigenetic changes, which regulate the homeostasis of the intestinal mucosa. Food-derived compounds are constantly present in the intestine and may shift cellular balance toward harmful outcomes, such as increased susceptibility to mutations. There is strong evidence that a major component of cancer risk may involve epigenetic changes in normal cells that increase the probability of cancer after genetic mutation. The recognition of epigenetic changes as a driving force in colorectal neoplasia would open new areas of research in disease epidemiology, risk assessment, and treatment, especially in mutation carriers who already have an inherited predisposition to cancer.

关键词：结肠癌食物 DNA 实验胚胎学营养学

Encapsulating metal organic framework into hollow mesoporous carbon sphere as efficient oxygen bifunctional electrocatalyst

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National Science Review 2020年第3期7卷 609-619页

作者： Wanfeng Xiong Hongfang Li Hanhui You minna Cao Rong Cao State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences College of Chemistry and Materials Science Fujian Normal University

Applying metal organic frameworks(MOFs) in electrochemical systems is a currently emerging field owing to the rich metal nodes and highly specific surface area of MOFs. However, the problems for MOFs that need to be solved urgently are poor electrical conductivity and low ion transport. Here we present a facile in situ growth method for the rational synthesis of MOFs@hollow mesoporous carbon spheres(HMCS)yolk–shell-structured hybrid material for the first time. The size of the encapsulated Zeolitic Imidazolate Framework-67(ZIF-67) is well controlled to 100 nm due to the spatial confinement effect of HMCS, and the electrical conductivity of ZIF-67 is also increased significantly. The ZIF@HMCS-25% hybrid material obtained exhibits a highly efficient oxygen reduction reaction activity with 0.823 V(vs. reversible hydrogen electrode) half-wave potential and an even higher kinetic current density(JK= 13.8 m A cm-2) than commercial Pt/C. ZIF@HMCS-25% also displays excellent oxygen evolution reaction performance and the overpotential of ZIF@HMCS-25% at 10 m A cm-2 is 407 m V. In addition, ZIF@HMCS-25% is further employed as an air electrode for a rechargeable Zn–air battery, exhibiting a high power density(120.2 m W cm-2 at 171.4 m A cm-2) and long-term charge/discharge stability(80 h at 5 m A cm-2). This MOFs@HMCS yolk–shell design provides a versatile method for the application of MOFs as electrocatalysts directly.

关键词： metal organic frameworks yolk–shell structure bifunctional electrocatalyst Zn–air battery

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山西省野生黄精资源调查

中国中医药现代远程教育 2024年第9期22卷 76-79页

作者：郭敏娜张孟容杨东方张玮玮孙靓徐丽霞山西药科职业学院中药系山西太原030031

目的调查山西省野生黄精分布状况及资源特点。方法采取资料收集、野外调查和实地走访等方法对山西省野生黄精资源进行收集整理分析。结果野生黄精资源全面覆盖山西省11个市,遍布50个县,吕梁和运城分布县域较多;海拔分布差异较大,344~250... 详细信息

目的调查山西省野生黄精分布状况及资源特点。方法采取资料收集、野外调查和实地走访等方法对山西省野生黄精资源进行收集整理分析。结果野生黄精资源全面覆盖山西省11个市,遍布50个县,吕梁和运城分布县域较多;海拔分布差异较大,344~2500 m均有涉及,主要集中在1100~2300 m;生长环境以草丛、山坡阴处、林下和灌木丛为主;叶片、果实和根茎形态存在差异:晋南部地区黄精叶、果整体比晋北地区偏大。其中,朔州叶长和叶宽均值最小,运城果实呈“圆球”形,大同果实偏“矮胖”,太原果实偏“瘦高”。结论山西省野生黄精资源集中分布在晋中和晋南地区,受地域影响,黄精之间形态特征存在较大差异,其潜在开发和利用价值较高。

关键词：黄精野生资源分布特点

The cost-effectiveness of hypertonic saline inhalations for infant bronchiolitis: a decision analysis

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World Journal of Pediatrics 2018年第1期14卷 26-34页

作者： Paula Heikkila minna Mecklin Matti Korppi Tampere Center for Child Health Research Universityof Tampere and Tampere University HospitalTampereFinland Department of Pediatrics Tampere University HospitalPOBOX200033521T ampereFinland

Background This study evaluated the cost-effectiveness of hypertonic saline (HS) inhalations for infant bronchiolitis, com-pared to normal saline inhalations or standard treatment without inhalations as controls. Methods The decision tree in the decision analysis was used to calculate the expected costs. Actual cost data were obtained from our retrospective case-control study on bronchiolitis treatment. The effectiveness of treatment, based on the hospitaliza-tion rate of those admitted to the emergency department and the length of stay (LOS) of those who were hospitalized, was collected from previous studies. For the effectiveness estimations, we made a meta-analysis summarizing the results of the meta-analysis of the Cochrane review in 2013 and the results of 10 studies published after it. Results The mean hospitalization rate was 24.7% in the HS inhalation group and 32.6% in the control group [risk ratio: 0.80, 95% confidence interval (CI) 0.67–0.96] and the mean LOS was 3.736 (HS group) and 4.292 (controls) days (mean difference: ? 0.55 days, 95% CI ? 0.96 to ? 0.15), respectively. The expected costs per patient, when both inpatients and outpatients were included, were €816 ($1111) in the HS inhalation group and €962 ($1310) in the control group. The expected costs per hospitalization, when only inpatients were included, were €2600 ($3540) in the HS inhalation group and €2890 ($3935) in the control group. Conclusions HS inhalations slightly reduced the expected hospitalization costs of infant bronchiolitis. However, the low effectiveness, rather than the cost, is the factor that will limit the use of HS inhalations in infant bronchiolitis.

关键词： Bronchiolitis Cost-effectiveness Decision analysis Hospitalization costs Hypertonic saline