检索结果-南通市图书馆

The Wnt-dependent and Wnt-independent functions of BCL9 in development,tumorigenesis,and immunity:Implications in therapeutic opportunities

在线全文

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第2期11卷 701-710页

作者： minjie Wu Heng Dong Chao Xu Mengqing sun Haojin Gao Fangtian Bu Jianxiang Chen College of Pharmacy and Department of Hepatology Institute of Hepatology and Metabolic DiseasesThe Affiliated Hospital of Hangzhou Normal UniversityHangzhou Normal UniversityHangzhouZhejiang 311121China Laboratory of Cancer Genomics Division of Cellular and Molecular ResearchNational Cancer CentreSingapore 169610Singapore Key Laboratory of Elemene Class Anti-Cancer Chinese Medicines Engineering Laboratory of Development and Application of Traditional Chinese MedicinesCollaborative Innovation Center of Traditional Chinese Medicines of Zhejiang ProvinceHangzhou Normal UniversityHangzhouZhejiang 311121China

B-cell CLL/lymphoma 9(BCL9)is considered a key developmental regulator and a well-established oncogenic driver in multiple cancer types,mainly through potentiating the Wnt/β-catenin signaling.However,increasing evidences indicate that BCL9 also plays multiple Wnt-independent roles.Herein,we summarized the updates of the canonical and non-canonical functions of BCL9 in cellular,physiological,or pathological processes.Moreover,we also concluded that the targeted inhibitors disrupt the interaction ofβ-catenin with BCL9 reported recently.

关键词： BCL9 Development Immunity Multifaceted functions Therapeutic target Tumorigenesis

Association between QRS voltages and amyloid burden in patients with cardiac amyloidosis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Journal 2024年第3期137卷 365-367页

作者： Jing-Hui Li Changcheng Li Yucong Zheng Kai Yang Yan Huang Huixin Zhang Xianmei Li Xiuyu Chen Linlin Dai Tian Lan Yang sun minjie Lu Shihua Zhao Department of Magnetic Resonance Imaging Fuwai HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China Department of Radiology Taian City Central Hospital(The Affiliated Taian City Central Hospital of Qingdao University)Tai’anShandong 271099China Department of Radiology Tsinghua University HospitalTsinghua UniversityBeijing 100084China Department of Radiology The Fifth People’s Hospital of Huai’anHuai’anJiangsu 223001China Department of Radiology Geriatric Hospital of Nanjing Medical UniversityNanjingJiangsu 210000China Department of Cardiology Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Department of Pathology Fuwai HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China

To the Editor:Cardiac amyloidosis(CA)is an infiltrative cardiomyopathy characterized by extracellular deposition of misfolded insoluble amyloid fibrils.^([1])Electrocardiography(ECG)is an essential diagnostic test characterized by low QRS voltages in CA,observed in 20–74%of patients.^([2])Cipriani et al^([3])reported that low QRS voltages could independently predict cardiovascular death.But the mechanisms underlying low QRS voltages in CA remain unclear.

关键词： amyloid patients death.

Reversing the PAI-1-induced fibrotic immune exclusion of solid tumor by multivalent CXCR4 antagonistic nano-permeator

在线全文

学校读者我要写书评

暂无评论

药学学报:英文版 2023年第7期13卷 3106-3120页

作者： Jingwen Dong Chenfei Zhu Ying Huang Quanhao Li Jing Li Zheng Wang Yixin Wang Zhanwei Zhou minjie sun NMPA Key Laboratory for Research and Evaluation of Pharmaceutical Preparations and Excipients State Key Laboratory of Natural MedicinesDepartment of PharmaceuticsChina Pharmaceutical UniversityNanjing 210009China

Fibrosis is one of the key factors that lead to the immune exclusion of solid tumors.Although degradation of fiber is a promising strategy,its application was still bottlenecked by the side effects of causing metastasis,resulting in the failure of immunotherapy.Here,we developed an antimetastatic polymer(HPA)for the delivery of chemo-drug and antifibrotic siPAI-1 to form the nano-permeator.Nano-permeator shrank after protonation and deeply penetrated into the tumor core to down-regulate the expression of PAI-1 for antifibrosis,and further promoted the sustained infiltration and activation of T cells for killing tumor cells.Moreover,metastasis after fiber elimination was prevented by multivalent CXCR4 antagonistic HPA to reduce the attraction of CXCL12 secreted by distant organs.The administration of stroma-alleviated immunotherapy increased the infiltration of CD8+T cells to 52.5%in tumor tissues,inhibiting nearly 90%metastasis by HPA in distant organs.The nano-permeator reveals the mechanism and correlation between antifibrosis and antimetastasis and was believed to be the optimizing immunotherapy for solid fibrotic tumors.

关键词： Immune exclusion PAI-1 Fibrosis Deep penetration AMD3100 CXCR4/CXCL12 Metastasis Immunotherapy

胎儿肺动脉瓣狭窄的超声心动图特征及生后管理分析

在线全文

学校读者我要写书评

暂无评论

中华儿科杂志 2024年第2期62卷 138-144页

作者：梁永州赵莉晴张敏杰陈笋孙锟武育蓉上海交通大学医学院附属新华医院儿童心脏中心上海200092

目的探讨胎儿不同程度肺动脉瓣狭窄(PS)的超声心动图特征、胎儿期与生后诊断一致性及生后结局。方法回顾性队列研究。选择2012年11月至2023年2月于上海交通大学医学院附属新华医院行胎儿期诊断并生后随访的108例PS胎儿为研究对象。收集P... 详细信息

目的探讨胎儿不同程度肺动脉瓣狭窄(PS)的超声心动图特征、胎儿期与生后诊断一致性及生后结局。方法回顾性队列研究。选择2012年11月至2023年2月于上海交通大学医学院附属新华医院行胎儿期诊断并生后随访的108例PS胎儿为研究对象。收集PS胎儿形态学及血流动力学等超声心动图特征,且所有胎儿至少随访至生后6月龄。采用单因素方差分析和Kruskal-Wallis检验等比较不同程度PS胎儿的超声心动图特征差异。采用McNemar检验分析胎儿期与生后诊断一致性。应用Logistic回归分析中度PS胎儿需新生儿期干预的危险因素,并利用受试者工作特征(ROC)曲线探索连续变量的最佳截断值。结果108例胎儿首诊时孕母年龄(30.8±4.0)岁,胎龄26.5(24.6,30.0)周。根据首诊超声心动图特征将胎儿PS分为轻度(17例)、中度(49例)和危重型(42例)。轻度PS胎儿超声心动图特征包括瓣膜增厚、回声增强伴流速增快或MPA扩张,中度PS同时伴瓣膜开放活动受限,瓣口呈五彩血流。中度和危重型PS胎儿过瓣流速明显快于轻度组[(2.66±0.86)和(2.77±1.30)比(1.43±0.59)m/s,F=14.52,P<0.001]。危重型PS均为动脉导管血流逆灌注,其右心室偏小比例明显高于轻度和中度组[42.9%(18/42)比0和2.0%(1/49),χ^(2)=31.73,P<0.001],且重度三尖瓣反流的比例也更高[35.7%(15/42)比0和10.2%(5/49),χ^(2)=36.94,P<0.001]。与轻度和危重型PS胎儿相比,中度PS胎儿期与生后诊断一致率更低[40.8%(20/49)比13/17和83.3%(35/42),χ^(2)=12.45,P=0.006]。过瓣流速是中度PS胎儿生后需新生儿期干预的独立危险因素(OR=7.21,95%CI 2.11~24.62),且胎儿中期过瓣流速≥2.18 m/s、胎儿晚期过瓣流速≥3.15 m/s提示中度PS胎儿需新生儿期干预。108例胎儿生后68例接受手术治疗后均存活,39例定期随访,1例未手术死亡,生后6个月存活率为99.1%(107/108)。结论不同程度的胎儿PS在超声心动图中表现出特征性的形态学和血流动力学改变。生后严重程度与胎儿期差异较大,应对胎儿PS行定期随访。胎儿PS的整体预后较好。

关键词：肺动脉瓣狭窄胎儿超声心动描记术

Development of fluorinated polyplex nanoemulsions for improved small interfering RNA delivery and cancer therapy

在线全文

学校读者我要写书评

暂无评论

Nano Research 2018年第7期11卷 3746-3761页

作者： Gang Chen Kaikai Wang Pengkai Wu Yixin Wang Zhanwei Zhou Lifang Yin minjie sun David Oupicky State Key Laboratory of Natural Medicines Departmen t of Pharmaceutics China Pharmaceutical University Nanjing 210009 China Center for Drug Delivery and Nanomedicine Department of Pharmaceutical Sciences University of Nebraska Medical Center Omaha NE 68198 USA

We report the development of a small interfering RNA （siRNA） delivery vector based on cationic perfluorocarbon nanoemulsions. We have prepared perfluorodecalin （PFD） emulsions with a positive surface charge provided by a fluorinated poly（ethylenimine）（F-PEI）. The fluorinated emulsion （F-PEI@PFD） reduced cytotoxicity of F-PEI and demonstrated effective binding with siRNAs to form nanosized emulsion polyplexes. The prepared emulsion polyplexes enhanced cellular uptake and improved endosomal escape of the siRNA. In addition to increased reporter gene silencing in multiple cancer cell lines, when compared with control F-PEI and PEI polyplexes, the siR_NA emulsion polyplexes showed an excellent resistance to serum deactivation and maintained high activity, even in high-serum conditions. The F-PEI@PFD emulsion polyplexes carrying an siRNA to silence the expression of Bcl2 gene induced apoptosis and inhibited tumor growth in a melanoma mouse model in vivo and showed potential for in vivo ultrasound imaging. This study demonstrates the potential of F-PEI@PFD emulsions as a multifunctional theranostic nanoplatform for safe siRNA delivery, with integrated ultrasound imaging functionality.

关键词： polyplexes fluorous interactions small interfering RNA（siRNA） delivery tumor deliver perfluorocarbon emulsion

A networked swellable dextrin nanogels loading Bcl2 siRNA for melanoma tumor therapy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2018年第9期11卷 4627-4642页

作者： Huipeng Li Zhanwei Zhou Feiran Zhang Yuxin Guo Xue Yang Hulin Jiang Fei Tan David Oupicky minjie sun State Key Laboratory of Natural Medicines and Department of Pharmaceutics China Pharmaceutical University Nanjing 210009 China Center for Drug Delivery and Nanomedicine Department of Pharmaceutical Sciences University of Nebraska Medical Center Omaha NE 68198 USA Shanghai Skin Disease Hospital Skin Disease Hospital of Tongji University Shanghai 200443 China

In this study, a networked swellable dextrin nanogel （DNG） was developed to achieve stimulated responsive small interfering RNA （siRNA） release for melanoma tumor therapy, siRNA was loaded into multidimensional dextrin nanogels by charge condensation with positive arginine residues modified in the dextrin backbone. Moreover, the networked nanogel was destroyed and loosened based on its bioreducible responsive property to control accelerated siRNA release in a bioreducible intracellular environment, while it remained stable under normal physiological conditions. We demonstrated that DNGs had swellable and disassembly properties under reduced buffer condition by transmission electron microscopy evaluation. The DNGs achieved an endosomal escape followed by selective release of the cargo into the cytosol by glutathione- triggered disassembly according to confocal microscopy observation. Thus, this smart nanogel achieved outstanding luciferase gene silencing efficiency and decreased Bcl2 protein expression in vitro and in vivo based on western blot analysis. Moreover, this nanogel exhibited superior anti-tumor activity for B16F10 xenograft tumors in C57BL/6 mice. These results demonstrate that the networked DNGs are effective for gene condensation and controlled intracellular release for tumor therapy. Overall, these findings suggest that this multidimensional swellable stimuli-responsive dextrin nanogel is an innovative strategy with great promise for gene and drug delivery.

关键词： dextrin nanogels local enrichment reduction-responsive lysosomal escape disulfide cross-linked small interfering RNA（siRNA） delivery

不同干燥方式对低盐腌制鲍鱼复水及品质特性的影响

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

食品工业科技 2024年第10期45卷 57-65页

作者：王舒娴杜瀚林端权翁凌曹敏杰孙乐常集美大学海洋食品与生物工程学院福建厦门361021 爱尔兰农业部农业与食品发展局Teagasc食品研究中心爱尔兰科克999014 水产品深加工技术国家地方联合工程中心福建厦门361021 海洋食品精深加工关键技术省部共建协同创新中心大连工业大学辽宁大连116034

为研究不同干燥方式对低盐腌制鲍鱼复水品质的影响,本研究采用真空冷冻干燥、冷风干燥与热风干燥对其进行干燥处理,研究其复水过程的质量体积和复水率,并通过低场核磁共振(low field nuclear magnetic resonance,LF-NMR)和磁共振成像(ma... 详细信息

为研究不同干燥方式对低盐腌制鲍鱼复水品质的影响,本研究采用真空冷冻干燥、冷风干燥与热风干燥对其进行干燥处理,研究其复水过程的质量体积和复水率,并通过低场核磁共振(low field nuclear magnetic resonance,LF-NMR)和磁共振成像(magnetic resonance imaging,MRI)研究复水过程的水分分布以及复水后的色差、质构、组织形态、游离氨基酸等变化情况。结果表明,在72 h时真空冷冻干鲍的复水率为278.73%,显著高于冷风干鲍和热风干鲍的复水率(P<0.05)。由LF-NMR和MRI结果分析得出,不易流动水和自由水含量是三种干鲍水分含量增加的主要因素。真空冷冻干鲍、冷风干燥和热风干鲍分别在72、48和24 h时复水完成。不同的干燥方式影响复水后的质构,结果显示,三种干燥方式的鲍鱼样品硬度与咀嚼性均有显著差异(P<0.05),真空冷冻干燥鲍鱼复水后硬度和咀嚼性最低,分别为954.01和708.59 g;热风干燥鲍鱼复水后硬度与咀嚼性最高,分别为1230.14和920.02 g。组织学染色与扫描电镜结果显示,真空冷冻干鲍的肌肉组织为较疏松的多孔结构;冷风干鲍和热风干鲍的组织为较细长和致密的结构。综上,真空冷冻干燥鲍鱼的复水率最高,硬度和咀嚼性最低,口感最好,但滋味不及热风干燥鲍鱼。本研究结果可为了解不同干燥方法对低盐腌制鲍鱼产品品质的影响提供理论参考。

关键词：鲍鱼复水品质特性干燥方式

维普期刊数据库博看期刊

蓝圆鲹分离蛋白酶解产物的制备及其抗大米淀粉老化特性

在线全文

学校读者我要写书评

暂无评论

水产学报 2024年第4期48卷 376-390页

作者：孙乐常孙小舒林端权陈玉磊翁凌缪松曹敏杰集美大学海洋食品与生物工程学院集美大学水产品深加工技术国家地方联合工程研究中心大连工业大学海洋食品精深加工关键技术省部共建协同创新中心爱尔兰农业部农业与食品发展局Teagasc食品研究中心

为了进一步拓展海洋鱼类蛋白在食品中的应用，以蓝圆鲹分离蛋白为原料，利用酶解改性得到溶解性良好的蓝圆鲹分离蛋白酶解物(BPIH)，并研究其对大米淀粉(RS)短期老化的抑制作用。通过响应面法优化酶解改性工艺制备BPIH，并分别按RS的3%... 详细信息

为了进一步拓展海洋鱼类蛋白在食品中的应用，以蓝圆鲹分离蛋白为原料，利用酶解改性得到溶解性良好的蓝圆鲹分离蛋白酶解物(BPIH)，并研究其对大米淀粉(RS)短期老化的抑制作用。通过响应面法优化酶解改性工艺制备BPIH，并分别按RS的3%、6%、9%(质量百分比)进行添加。通过测定RS的凝沉性、动态黏弹性、热学特性以及微观结构分析评价BPIH的抗淀粉老化活性。结果显示，响应面法确定的最佳条件：酶底比5 000∶1 (U/g)、酶解时间3 h、料液比1.00∶3.81、酶解温度46.36℃、酶解pH 6.30，氮溶指数(NSI)实际值为85.41%±0.82%，与预测值86.37%接近。该酶解条件下BPIH水解度达到21.62%。BPIH中小于1 000 u的肽占79.94%，主要为小分子低聚寡肽。加入BPIH可以减弱RS的凝沉现象，降低RS在4℃保存过程中的储能模量(G')，显著降低老化后RS的峰值温度(Tp)和焓值(ΔHr)；加入BPIH的大米淀粉，老化后微观结构有较大孔洞，提升了淀粉糊化后保留内部水分的能力。研究表明，BPIH能够抑制RS在4℃保存期间凝胶网络和微晶结构的形成，同时可能限制了淀粉分子间的聚集，抑制或延缓RS的短期老化。本研究为蓝圆鲹分离蛋白酶解物在食品蛋白配料中的应用提供理论参考。

关键词：蓝圆鲹分离蛋白酶解淀粉短期老化

制造业中小企业数字化改造实践探析——以浙江省嘉兴市为例

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

新型工业化 2024年第5期14卷 31-37页

作者：杨康孙敏杰李鑫嘉兴市经济和信息化局嘉兴314000 南方科技大学嘉兴研究院嘉兴314000

新型工业化背景下,量大面广的中小企业由于“缺资金、缺技术、缺人才”,导致“不想转、不敢转、不会转”,成为困扰各级政府推动产业转型升级的普遍性难题。近年来,浙江省嘉兴市全面推进数字化绿色化“两化”改造,有效提升了全市制造业... 详细信息

新型工业化背景下,量大面广的中小企业由于“缺资金、缺技术、缺人才”,导致“不想转、不敢转、不会转”,成为困扰各级政府推动产业转型升级的普遍性难题。近年来,浙江省嘉兴市全面推进数字化绿色化“两化”改造,有效提升了全市制造业的高端化、智能化、绿色化水平,特别是有效推进了一批制造业重点细分行业中小企业数字化改造,促使数字化改造项目数量稳步增长和生产效率等改造成效指标显著提升。本文简要阐述了推进制造业中小企业数字化改造的重要意义,梳理了嘉兴市相关经验做法,研究了存在的问题,并提出了对策建议,可为其他地区制造业数字化转型升级提供有益的参考。

关键词：新型工业化数字化制造业中小企业