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Morphological properties and proliferation analysis of olfactory ensheathing cells seeded onto three-dimensional collagen-heparan sulfate biological scaffolds

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Neural Regeneration Research 2012年第16期7卷 1213-1219页

作者： Na Liu Zhouping Tang Zhiyuan Yu minjie xie Yu Zhang Erfang Yang Shabei Xu Department of Neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 HubeiProvince China

This study aimed to examine the differences in the morphological properties and proliferation of olfactory ensheathing cells in three-dimensional culture on collagen-heparan sulfate biological scaffolds and in two-dimensional culture on common flat culture plates. The proliferation rate of olfactory ensheathing cells in three-dimensional culture was higher than that in two-dimensional culture, as detected by an M-I-r assay. In addition, more than half of the olfactory ensheathing cells subcultured using the trypsinization method in three-dimensional culture displayed a spindly Schwann cell-like morphology with extremely long processes, while they showed a flat astrocyte-like morphology in two-dimensional culture. Moreover, spindle-shaped olfactory ensheathing cells tended to adopt an elongated bipolar morphology under both culture conditions. Experimental findings indicate that the morphological properties and proliferation of olfactory ensheathing cells in three-dimensional culture on collagen-heparan sulfate biological scaffolds are better than those in two-dimensional culture.

关键词： morphological properties cell proliferation biological scaffold olfactory ensheathing cells three-dimensional culture neural regeneration

Rho-Associated Kinase Inhibitors Promote Microglial Uptake Via the ERK Signaling Pathway

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Neuroscience Bulletin 2016年第1期32卷 83-98页

作者： Peicai Fu Ronghua Tang Zhiyuan Yu Caihong Li Xue Chen minjie xie Wei Wang Xiang Luo Department of Neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Key Laboratory of Neurological Diseases (HUST) Ministry of Education of China

Microglia are immunocompetent cells in the cen- tral nervous system that take up tissue debris and pathogens. Rho-associated kinase （ROCK） has been identified as an important regulator of uptake, proliferation, secretion, and differentiation in a number of cell types. Although ROCK plays critical roles in the microglial secretion of inflammatory factors, naigration, and morphology, its effects on microglial uptake activity have not been well characterized. In the present study, we found that treatment of BV2 microglia and primary microglia with the ROCK inhibitors Y27632 and fasudil increased uptake activity and was associated with morpholog- ical changes. Furthermore, western blots showed that this increase in uptake activity was mediated through the extracel- lular-signal-regulated kinase （ERK） signaling cascade, indi- cating the importance of ROCK in regulating microglial uptake activity.

关键词： Microglia Rho-associated kinase Uptakeactivity Extracellular-signal-regulated kinase

Lattice Vector Quantization Applied to Speech and Audio Coding

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ZTE Communications 2012年第2期10卷 25-33页

作者： minjie xie ZTE USA Inc. Richardson TX 75080 USA

Lattice vector quantization （LVQ） has been used for real-time speech and audio coding systems. Compared with conventional vector quantization, LVQ has two main advantages： It has a simple and fast encoding process, and it significantly reduces the amount of memory required. Therefore, LVQ is suitable for use in low-complexity speech and audio coding. In this paper, we describe the basic concepts of LVQ and its advantages over conventional vector quantization. We also describe some LVQ techniques that have been used in speech and audio coding standards of international standards developing organizations （SDOs）.

关键词： Vector quantization lattice vector quantization speech and audio coding transform coding

Digoxin Ameliorates Glymphatic Transport and Cognitive Impairment in a Mouse Model of Chronic Cerebral Hypoperfusion

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Neuroscience Bulletin 2022年第2期38卷 181-199页

作者： Jie Cao Di Yao Rong Li Xuequn Guo Jiahuan Hao minjie xie Jia Li Dengji Pan Xiang Luo Zhiyuan Yu Minghuan Wang Wei Wang Department of Neurology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Pediatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Respiratory Medicine Quanzhou First Hospital Affiliated to Fujian Medical UniversityQuanzhou 362000China Key Laboratory of Neurological Diseases of the Chinese Ministry of Education School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

The glymphatic system plays a pivotal role in maintaining cerebral *** cerebral hypoperfusion,arising from small vessel disease or carotid stenosis,results in cerebrometabolic disturbances ultimately manifesting in white matter injury and cognitive ***,whether the glymphatic system serves as a potential therapeutic target for white matter injury and cognitive decline during hypoperfusion remains ***,we established a mouse model of chronic cerebral hypoperfusion via bilateral common carotid artery *** found that the hypoperfusion model was associated with significant white matter injury and initial cognitive impairment in conjunction with impaired glym・phatic system *** glymphatic dysfunction was associated with altered cerebral perfusion and loss of aquaporin 4 *** of digoxin rescued changes in glymphatic transport,white matter structure,and cognitive *** of glymphatic functions by treatment with the AQP4 inhibitor TGN-020 abolished this protective effect of digoxin from hypoperfusion *** research yields new insight into the relationship between hemodynamics,glymphatic transport,white matter injury,and cognitive changes after chronic cerebral hypoperfusion.

关键词： Chronic cerebral hypoperfusion Cognitive impairment Digoxin Glymphatic system White matter injury

The expression of TRPA1 mRNA in the rat brain

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Journal of Nanjing Medical University 2006年第4期20卷 225-228页

作者： Peng Du Shua Li Jinyu Zheng Zhi-yuan Yu minjie xie Wei Wang Department of Neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Shanghai Institute of Neuroscience Shanghai 200030China Department of Pathology Drum Tower Hospital Nanjing University Nanjing210008 China

Objective： To investigate the distribution of TRPA1 （one kind of the TRP-like ion channel family） channel in the hippocampus and cerebral cortex of rat. Methods： RT-PCR was used to amplify the fragment of TRPA1 in the DRG （dorsal root ganglion）, hippocampus and cerebral cortex of adult SD rat. In situ hybridization staining was used to show the distribution of TRPA1 mRNA in the hippocampus and cerebral cortex of adult rat brain. Results： Both RT-PCR and in situ hybridization staining showed that TRPA1 mRNA was expressed in hippocampus and cerebral cortex of the adult rat brain. Conclusion： Our results suggest that there is expression of TRPA1 mRNA both in the hippocampus and cerebral cortex of the adult rat brain.

关键词： TRPA1 RT-PCR in situ hybridization hippocampus cerebral cortex rat

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汽油机油高温氧化性能试验方法的研究

合成润滑材料 2015年第3期42卷 12-15页

作者：颉敏杰中国石油兰州润滑油研发中心兰州730060

参照IP48润滑油氧化性能试验方法,研究了一种评价汽油机油高温氧化性能的简易试验方法。该方法氧化漆膜与ⅢE台架试验的油环岸沉积物的相关系数为0.63,氧化后40℃运动黏度增长与ⅢE台架试验的40℃运动黏度增长的相关系数达到了0.89。

关键词：汽油机油高温氧化性能试验方法 ⅢE台架试验相关系数

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某大斜交角度框架地道桥的设计与分析

石家庄铁道大学学报（自然科学版） 2012年第2期25卷 15-18页

作者：谢敏杰中铁咨询郑州设计院河南郑州450001

林邓线焦作市界至月山段一级公路下穿新月铁路下行线5#框架地道桥,斜交角度为48.3°,受力情况复杂;为改善斜交扭转,提高铁路建筑物的使用寿命,保证铁路后期运营安全,地道桥与铁路线路按正交设计,左右两幅路分别采用(8.5 m+8.5 m+8.5 m)... 详细信息

林邓线焦作市界至月山段一级公路下穿新月铁路下行线5#框架地道桥,斜交角度为48.3°,受力情况复杂;为改善斜交扭转,提高铁路建筑物的使用寿命,保证铁路后期运营安全,地道桥与铁路线路按正交设计,左右两幅路分别采用(8.5 m+8.5 m+8.5 m)三孔连续框构形式。同时利用Sap2000有限元软件采用三维空间板壳单元对该地道桥进行分析,提出了分析时应该注意的几个问题,得到了比较合理的结果,明确了框架地道桥顶底板的结构受力特点,为以后的设计提供参考。

关键词：大斜交角度框架地道桥正交设计三维空间板壳单元

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节能汽油机油的评价

合成润滑材料 2007年第4期34卷 23-26页

作者：颉敏杰彭琼中国石油兰州润滑油研发中心兰州730060 兰州石化公司炼油厂兰州730060

介绍了节能汽油机油的发展和汽油机油燃油经济性的台架评价方法。

关键词：汽油机油燃油经济性评价方法

ZnCl_2阻断电压门控质子通道对缺氧后小胶质细胞产生活性氧及促炎因子的影响

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中国细胞生物学学报 2016年第7期38卷 829-835页

作者：余颖喻志源谢敏杰王伟骆翔华中科技大学同济医学院附属同济医院神经内科武汉430030

为明确电压门控质子通道(voltage-gated proton channel,Hv1)对小胶质细胞的影响,研究了Hv1在正常、激活及抑制后对小胶质细胞活性氧、促炎因子产生的影响及机制,该研究使用原代培养的小胶质细胞,利用免疫荧光染色明确Hv1表达。用Hv1非... 详细信息

为明确电压门控质子通道(voltage-gated proton channel,Hv1)对小胶质细胞的影响,研究了Hv1在正常、激活及抑制后对小胶质细胞活性氧、促炎因子产生的影响及机制,该研究使用原代培养的小胶质细胞,利用免疫荧光染色明确Hv1表达。用Hv1非特异性抑制剂Zn Cl2阻断该通道,用DCFH-DA染色、Real-time PCR观察糖氧剥夺(oxygen glucose deprivation,OGD)处理后小胶质细胞产生活性氧(reactive oxygen species,ROS)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)水平的变化。采用Western blot检测Hv1和Nox2蛋白质水平。实验结果显示,小鼠脑部小胶质细胞可检测到Hv1通道的表达;单纯ODG组缺氧后小胶质细胞ROS、TNF-α、IL-1β的产生明显增加;而OGD加Zn Cl2干预组,小胶质细胞ROS、TNF-α、IL-1β的产生较单纯OGD组显著降低;Western blot显示,OGD导致小胶质细胞中Hv1和Nox2蛋白质水平显著增加。以上结果表明,小鼠脑部小胶质细胞存在Hv1通道蛋白质,Zn Cl2阻断该通道可降低OGD诱导的小胶质细胞ROS和TNF-α、IL-1β的产生,该抑制作用可能与其抑制NADPH氧化酶的作用相关。

关键词：小胶质细胞电压门控质子通道活性氧促炎因子