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Discovery of novel double pyrazole Schiff base derivatives as anti-tobacco mosaic virus(TMV) agents

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Chinese Chemical Letters 2017年第2期28卷 377-382页

作者： Xian-Hai Lv Zi-Li Ren dong-dong Li Ban-Feng Ruan Qing-Shan Li Ming-Jie Chu Cheng-Ying Ai dao-Hong Liu Kai Mo Hai-Qun Cao School of Plant Protection Anhui Agricultural University Hefei 230036 China School of Biological and Medical Engineering Hefei University of Technology Hefei 230009 China School of Chemical Engineering Nanjing Forestry University Nanjing 210073 China State Key Laboratory of Pharmaceutical Biotechnology Nanjing University Nanjing 210093 China

Many pyrazole derivatives were reported to exhibit highly activity towards tobacco mosaic virus（TMV）.In this work,an optimized pyrazole Schiff base scaffold was designed and introduced to derive novel potential TMV ***-six compounds were synthesized,characterized by elemental analysis,mass spectra and nuclear magnetic resonance（NMR） spectroscopy and evaluated by biological *** bioassay results showed that some of the synthesized compounds exhibited excellent anti-TMV ***,5-chloro-3-methyl-1H-pyrazole contained compound 4j showed ningnanmycin comparable inhibitory activity and can be considered as potential anti-TMV candidate *** molecular docking,compound 4j insert into nucleotide sequence（GAAGUU） of OriRNA stably which revealed nucleotide could be a target of these compounds.

关键词： Pyrazole Schiff base Anti-TMV Molecule docking

Effects of Organic Manure Application with Chemical Fertilizers on Nutrient Absorption and Yield of Rice in Hunan of Southern China

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Agricultural Sciences in China 2008年第10期7卷 1245-1252页

作者： XU Ming-gang LI dong-chu LI Ju-mei QIN dao-zhu Kazuyuki Yagi Yasukazu Hosen Key Laboratory of Plant Nutrition and Nutritional Cyclying Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Crop Production and Environment Division Japan International Research Center for Agricultural Sciences 1-1 Ohwashi Tsukuba Ibaraki 305-8686 Japan

To evaluate the effect of organic manure application with chemical fertilizers on rice yield and soil fertility under long-term double-rice cropping system, a six year field experiment was conducted continually in the paddy soil derived from Quaternary red clay in Hunan Province of southern China. Four different treatments, i.e., no nitrogen with chemical P and K （PK）, swine manure only （M）, N, P and K chemical fertilizers only （NPK）, and half chemical fertilizers combined with half swine manure （NPKM） with four replications were included. Each N, P and K application rate was the same at all the treatments （except the N application rate at PK） and N application rate was 150 kg N ha^-1. All fertilizers were applied to soil tillage layer with once application as baseal fertilizers. The nutrients uptake rate, grain yield, nitrogen use efficiency, and soil organic matter content at each treatment were investigated. The NPKM treatment achieved the highest mean annual yield of 12.2 t ha^-1 （68% higher than that of PK）. Higher dry matter accumulation and nutrients absorption were observed during the middle-late growth period in the NPKM treatment, with higher panicle number per unit and filled-grain number per panicle. Its average nitrogen use efficiency was 36.3% and soil organic matter increased by 18.5% during the experimental period in the NPKM treatment, which were significantly higher than those in the NPK treatment. Organic manure application with chemical fertilizers increased the yield and nitrogen use efficiency of rice, reduced the risk of environmental pollution and improved soil fertility greatly. It could be a good practical technique that protects the environment and raises the rice yield in this region.

关键词： rice chemical fertilizer organic manure nutrient absorption yield

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湖南甘薯连作障碍缓解肥的筛选及其应用效果

湖南农业科学 2024年第9期 68-72页

作者：康世东黄艳岚张亚董芳刘红梅张超凡张道微湖南省农业科学院作物研究所湖南长沙410125 湖南农业大学农学院湖南长沙410128 岳麓山实验室湖南长沙410128

为缓解湖南省甘薯主产区存在的连作障碍问题,研究以湖南省典型的酸壤型连作障碍地为试验区,以湘薯203和徐紫薯8号为试验品种,分别施用等效价的甘薯专用复合肥、丘陵旱薄地控钾型腐殖酸控释肥、平原地控钾型腐殖酸控释肥、腐殖酸复合肥... 详细信息

为缓解湖南省甘薯主产区存在的连作障碍问题,研究以湖南省典型的酸壤型连作障碍地为试验区,以湘薯203和徐紫薯8号为试验品种,分别施用等效价的甘薯专用复合肥、丘陵旱薄地控钾型腐殖酸控释肥、平原地控钾型腐殖酸控释肥、腐殖酸复合肥、生物有机无机复混肥、生物菌肥A、生物菌剂B进行处理,比较分析了甘薯连作区施用不同类型肥料处理后甘薯产量和土壤养分的差异。结果表明:(1)湘薯203试验中,施用不同类型的肥料均能在一定程度上提升甘薯产量,其中生物有机无机复混肥处理区的实际鲜薯产量可达40.15 t/hm^(2),比专用复合肥处理的对照组增产37.69%,生物总量增加22.63%,增产效果最显著;腐殖酸复合肥处理与生物有机无机复混肥处理的增产效果接近,鲜薯产量较对照提升35.70%,生物总量增加18.68%。(2)徐紫薯8号试验中,生物有机无机复混肥处理区鲜薯实际产量可达46.95 t/hm^(2),比专用复合肥处理的对照组增产11.68%,生物总量增加7.38%;腐殖酸复合肥处理区实际鲜薯产量较对照CK1下降5.04%,生物总量增加19.15%。(3)不同施肥处理改变了土壤地力情况,生物有机无机复混肥或腐殖酸施用区甘薯对连作障碍地养分平衡利用的效果最佳;专用复合肥处理区虽然土壤养分明显增加,但是甘薯对养分的利用率并不理想。综上所述,施用以生物有机无机复混肥、腐殖酸复合肥这类含有机质的肥料处理对湖南甘薯连作障碍地鲜薯产量的提升最有效。

关键词：甘薯肥效试验连作障碍鲜薯产量土壤养分利用

Nucleic acid detection in the diagnosis and prevention of schistosomiasis

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Infectious Diseases of Poverty 2016年第1期5卷 230-240页

作者： Ping He Lan-gui Song Hui Xie Jin-yi Liang dong-ya Yuan Zhong-dao Wu Zhi-yue Lv Department of Parasitology Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080China Key Laboratory of Tropical Disease Control Ministry of EducationGuangzhou 510080China Department of Pathogenic Biology Medical SchoolXizang Minzu UniversityXianyang 712082China

Schistosomiasis is an important zoonotic parasitic disease that causes serious harms to humans and *** and diagnosis play key roles in schistosomiasis control,however,current techniques for surveillance and diagnosis of the disease have *** genome data for parasites are increasing,novel techniques for detection incorporating nucleotide sequences are receiving widespread *** sensitive,specific,and rapid detection methods are particularly important in the diagnosis of low-grade and early infections,and may prove to have clinical *** paper reviews the progress of nucleic acid detection in the diagnosis and prevention of schistosomiasis,including such aspects as the selection of target genes,and development and application of nucleic acid detection methods.

关键词： Schistosoma japonicum S.mansoni S.haematobium Diagnosis Nucleic acid detection

Characteristics of debris flow impact on a double-row slit dam

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Journal of Mountain Science 2023年第2期20卷 415-428页

作者： WANG Zhuang LIU dao-chuan YOU Yong LYU Xiao-bo LIU Jin-feng ZHAO Wan-yu SUN Hao WANG dong-wei LIU Yang Key Laboratory of Mountain Hazards and Earth Surface Process Institute of Mountain Hazards and EnvironmentChinese Academy of SciencesChengdu 610041China University of Chinese Academy of Sciences Beijing 100049China Sichuan Highway Planning SurveyDesign and Research Institute Ltd.Chengdu 610041China Sichuan Tongchuan Engineering Technology Development Co. Ltd.Chengdu 610041China State Grid Sichuan Electric Power Transmission&Transformation Construction Co. Ltd.Chengdu 610051China

To accurately predict impact loads can ensure the safe operation of debris flow control *** instantaneous impact process is usually considered in the calculation of the debris flow impact force;however,the redistribution of an impact load after structural regulation is *** this study we deduced the theoretical calculation of a debris flow impact on a double-row slit dam,and carried out a verification experiment on the debris flow *** calculation model considers the influence of the debris flow properties,dam arrangement and pile *** results show that the impact force of the debris flow is obviously affected by the bulk *** the bulk density is 21 kg/m^(3),the maximum impact force on the pile dam is 1.15 times that when the bulk density is 15 kg/m^(3),but the time it takes for the debris flow to pass through the dam body is reduced by 60%.The larger the relative pile spacing,the more sufficient the flow space and the lower the maximum impact *** maximum impact force of relative pile spacing of 0.8 is 12%less than that of elative pile spacing of *** horizontal distribution of the impact force in the mud depth range is *** maximum impact force on the centre pier is 1.3 times that of a side pier,and the maximum impact force on the dam body appears at the top of the mud depth *** the vertical distribution of the impact force,the maximum impact force at the highest mud mark is approximately 70%of that of the *** the increase in the relative pile spacing,the longitudinal maximum impact force distribution first decreases and then increases.

关键词： Debris flow force Slit dam Fluctuations Flume model test

意大利蜜蜂工蜂幼虫肠道发育过程中lncRNA的表达谱和调控作用

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昆虫学报 2024年第8期67卷 1050-1062页

作者：赵浩东臧贺叶道有陈颖王宁吴鹰蒋海宾冯睿蓉陈大福熊翠玲郭睿福建农林大学蜂学与生物医药学院福州350002 天然生物毒素国家地方联合工程实验室福州350002 福建省蜂疗研究所福州350002 吉林省养蜂科学研究所吉林132000 福建农业职业技术学院福州350000

【目的】解析意大利蜜蜂Apis mellifera ligustica工蜂幼虫肠道发育过程中的长链非编码RNA(long non-coding RNA,lncRNA)差异表达谱,并揭示差异表达lncRNA(differentially expressed lncRNA,DElncRNA)在幼虫肠道发育中的调控作用。【方法】基于前期获得的意大利蜜蜂工蜂4,5和6日龄幼虫肠道转录组数据(分别为Am4,Am5和Am6),利用相关软件筛选Am4 vs Am5比较组和Am5 vs Am6比较组中的DElncRNA,分析DElncRNA和两个比较组中共同上调和下调lncRNA的顺式调控作用及竞争性内源RNA(competing endogenous RNA,ceRNA)的调控作用。通过RT-qPCR验证转录组数据的可靠性。【结果】在Am4 vs Am5比较组中筛选出214条上调和251条下调lncRNA,在Am5 vs Am6比较组中筛选出141条上调和332条下调lncRNA;两个比较组共有的上调和下调lncRNA分别为7和16条。Am4 vs Am5比较组中的DElncRNA潜在调控250个邻近基因,涉及细胞进程等28个GO条目及Wnt信号通路等58条KEGG通路;Am5 vs Am6比较组中的DElncRNA潜在调控295个邻近基因,涉及细胞部分等35个GO条目及FoxO信号通路等73条KEGG通路;上述两个比较组中共有的7个上调lncRNA潜在调控10个邻近基因,涉及1个GO条目及代谢通路、谷胱甘肽代谢和核质转运等7条KEGG通路。共有的16个下调lncRNA潜在调控27个邻近基因,涉及8个GO条目及精氨酸生物合成、谷胱甘肽代谢和代谢通路等13条KEGG通路。此外,Am4 vs Am5比较组中的49条DElncRNA可靶向16个差异表达miRNA(differentially expressed miRNA,DEmiRNA)进而靶向122条差异表达mRNA(differentially expressed mRNA,DEmRNA),可注释到代谢进程等24个GO条目和Wnt信号通路等21条KEGG通路。Am5 vs Am6比较组中的38条DElncRNA可靶向8条DEmiRNA进而靶向67条DEmRNA,可注释到催化活性等21个GO条目和FoxO信号通路等10条KEGG通路;上述两个比较组共有的1条下调lncRNA MSTRG.10589.2可靶向ame-miR-6052和miR-511-y,进而靶向29条DEmRNA。RT-qPCR结果显示随机选取的7条DElncRNA的相对表达量与测序数据一致,证实了所用转录组数据的可靠性。【结论】意大利蜜蜂工蜂幼虫肠道发育过程伴随着lncRNA的动态差异表达,DElncRNA可通过顺式作用和ceRNA网络潜在参与对幼虫肠道发育的调控,在幼虫肠道发育中潜在发挥重要的调控作用。

关键词：意大利蜜蜂工蜂幼虫长链非编码RNA 肠道发育

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Protection-Free Site-Directed Peptide or Protein S-Glycosylation and Its Application in the Glycosylation of Glucagon-like Peptide 1

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CCS Chemistry 2022年第6期4卷 1930-1937页

作者： Gefei Li Yuankun dao Juan Mo Suwei dong Shin-ichiro Shoda Xin-Shan Ye State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191 Department of Biomolecular Engineering Graduate School of EngineeringTohoku UniversityAoba-kuSendai 980-8579

The development of a facile cysteine-directed S-glycosylation strategy would facilitate the intensive investigation of the effect of glycosylation on protein translational ***,we introduce glycosyl Bunte salt as an efficient glycosyl donor for site-selective peptide/protein *** coupling reaction with cysteine thiols under alkaline buffer conditions proceeded chemoselectively,delivering homogeneous glycoconjugates and inorganic salt as the only byproduct.A series of sugar moieties,including monosaccharides and oligosaccharides,were successfully conjugated to the peptides and protein via the disulfide ***,this protocol was applied to the glycosylation of the glucagon-like peptide 1(GLP-1)variant,and its glycosylated effect on blood glucose control was also studied.

关键词： site-selective S-glycosylation peptide/protein-modification glycosyl Bunte salt disulfide bond GLP-1

Winter AO/NAO Modifies Summer Ocean Heat Content and Monsoonal Circulation over the Western Indian Ocean

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Journal of Meteorological Research 2017年第1期31卷 94-106页

作者： dao-Yi GONG dong GUO Sang LI Seong-Joong KIM State Key Laboratory of Earth Surface Processes and Resource Ecology Beijing Normal University Climate Change Research Center Chinese Academy of Sciences Division of Polar Climate Change Korea Polar Research Institute

This paper analyzes the possible influence of boreal winter Arctic Oscillation/North Atlantic Oscillation （AO/ NAO） on the Indian Ocean upper ocean heat content in summer as well as the summer monsoonal circulation. The strong interannual co-variation between winter 1000-hPa geopotential height in the Northern Hemisphere and sum- mer ocean heat content in the uppermost 120 m over the tropical Indian Ocean was investigated by a singular decom- position analysis for the period 1979-2014. The second paired-modes explain 23.8% of the squared covariance, and reveal an AO/NAO pattern over the North Atlantic and a warming upper ocean in the western tropical Indian Ocean. The positive upper ocean heat content enhances evaporation and convection, and results in an anomalous meridional circulation with ascending motion over 5°S-5°N and descending over 15°-25°N. Correspondingly, in the lower tro- posphere, significantly anomalous northerly winds appear over the western Indian Ocean north of the equator, imply- ing a weaker summer monsoon circulation. The off-equator oceanic Rossby wave plays a key role in linking the AO/NAO and the summer heat content anomalies. In boreal winter, a positive AO/NAO triggers a down-welling Rossby wave in the central tropical Indian Ocean through the atmospheric teleconnection. As the Rossby wave ar- rives in the western Indian Ocean in summer, it results in anomalous upper ocean heating near the equator mainly through the meridional advection. The AO/NAO-forced Rossby wave and the resultant upper ocean warming are well reproduced by an ocean circulation model. The winter AO/NAO could be a potential season-lead driver of the sum- mer atmospheric circulation over the northwestern Indian Ocean.

关键词： Arctic Oscillation North Atlantic Oscillation Indian Ocean upper ocean heat content summer mon-soonal circulation

食源性降血脂肽的研究进展:来源、作用机制及其构效关系

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食品安全质量检测学报 2024年第6期15卷 124-132页

作者：王静静范硕潘道东曾小群吴振杜启伟涂茂林宁波大学食品科学与工程学院浙江省动物蛋白食品精深加工技术重点实验室宁波315800

高脂血症是全球常见疾病之一,会引发一系列其他相关疾病,严重威胁人体健康。他汀类药物广泛用于治疗高脂血症,但长期服用会引发胃肠道疾病等,对人体健康造成负担。因此,开发研究更为安全健康的新型天然活性成分很重要。近年来,从食源性... 详细信息

高脂血症是全球常见疾病之一,会引发一系列其他相关疾病,严重威胁人体健康。他汀类药物广泛用于治疗高脂血症,但长期服用会引发胃肠道疾病等,对人体健康造成负担。因此,开发研究更为安全健康的新型天然活性成分很重要。近年来,从食源性蛋白中制备能有效降低血脂水平的生物活性肽逐渐受到人们的关注。食源性降血脂肽主要通过降低胆固醇、甘油三脂,抑制胰脂肪酶、脂肪酸合成酶等方式达到降血脂效果,维护机体健康,相较于药物其副作用小、更加安全。本文就食源性降血脂肽的来源进行分类,并阐述了其作用机制和构效关系的最新研究进展,对其未来研究发展趋势进行展望,以期为降血脂肽的深入研究和相关功能性食品的开发提供参考。

关键词：高脂血症降血脂肽构效关系活性机制