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Transcriptome-Wide Identification and Functional Analysis of PgSQE08-01 Gene in Ginsenoside Biosynthesis in Panax ginseng ***.

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Phyton-International Journal of Experimental Botany 2024年第2期93卷 313-327页

作者： Lei Zhu Lihe Hou Yu Zhang Yang Jiang Yi Wang Meiping Zhang mingzhu zhao Kangyu Wang College of Life Science Jilin Agricultural UniversityChangchun130118China Jilin Engineering Research Center Ginseng Genetic Resources Development and Utilization Changchun130118China Jilin Academy of Vegetable and Flower Sciences Changchun130033China

Panax ginseng *** an important plant species used in traditional Chinese medicine,whose primary active ingredient is a *** biosynthesis is not only regulated by transcription factors but also controlled by a variety of structural ***,the molecular mechanism underlying ginsenoside biosynthesis has always been a topic in the discussion of ginseng secondary *** epoxidase(SQE)is a key enzyme in the mevalonic acid pathway,which affects the biosynthesis of secondary metabolites such as *** ginseng transcriptome,expression,and ginsenoside content databases,this study employed bioinformatic methods to systematically analyze the genes encoding SQE in *** first selected six PgSQE candidates that were closely involved in ginsenoside biosynthesis and then identified PgSQE08-01 to be highly associated with ginsenoside ***,we constructed the overexpression vector pCAMBIA3301-PgSQE08-01 and the RNAi vector pART27-PgSQE08-01 and transformed ginseng adventitious roots using Agrobacterium rhizogenes,to obtain positive hairy-root ***,quantitative reverse transcriptionpolymerase chain reaction and high-performance liquid chromatography were used to determine the expression of relevant genes and ginsenoside content,***,we focused on the function of PgSQE08-01 gene,which was noted to be involved in ginsenoside ***,these findings not only provided a molecular basis for the identification of important functional genes in ginseng but also enriched genetic resources for the biosynthesis of ginsenosides using synthetic biology.

关键词： Panax ginseng pgSQE08-01 gene squalene epoxidase ginsenoside ginseng hairy roots

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A new deformable cable for rock support in high stress tunnel: Steel pipe shrinkable energy-absorbing cable

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International Journal of Mining Science and Technology 2024年第08期 1083-1093页

作者： Xuezhen Wu mingzhu zhao Qing Ye Yujing Jiang Tao Deng Hanfang Zheng Gang Wang Zhenchang Guan College of Civil Engineering Fuzhou University School of Engineering Nagasaki University Key Laboratory of Underground Engineering Fujian University of Technology

High stress in surrounding rock will lead to serious problems, e.g., rock burst in hard rock and large deformation in soft rock. The applied support system under high in-situ stress conditions should be able to carry high load and also accommodate large deformation without experiencing severe damage. In this paper, a specially designed energy-absorbing component for rock bolt and cable that can solve the above problems was proposed. The energy-absorbing component can provide support resistance by plastic deformation of the metal including constraint annulus and compression pipe. For practical engineering,two forms were proposed. One was installed in the surrounding rock by reaming, and the other was installed directly outside the surrounding rock. During the dilation of the surrounding rock, the relative displacement of constraint annulus and compression pipe occurs, resulting in deformation *** resistance is transmitted to the rock bolt or cable, providing support resistance. The lab test and numerical simulation showed that the energy-absorbing component can perfectly achieve the large deformation effect, the deformation amount is as high as 694 mm, and the bearing capacity is stable at367 k N. The field application tests were carried out in the mining roadway of Xinjulong coal mine, and the results showed that the new type of cable can ensure itself not to break under the condition of large deformation of the surrounding rock. The energy-absorbing component has the superiorities of performing large constant resistance and controllable deformation to effectively control the unpredictable disasters such as large deformation in soft rock and rock burst in hard rock encountered in deep strata.

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Discovery of a potent and highly selective inhibitor of SIRT6 against pancreatic cancer metastasis in vivo

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Acta Pharmaceutica Sinica B 2024年第3期14卷 1302-1316页

作者： Xinyuan Xu Qian Zhang Xufeng Wang Jing Jin Chengwei Wu Li Feng Xiuyan Yang mingzhu zhao Yingyi Chen Shaoyong Lu Zhen Zheng Xiaobing Lan Yi Wang Yan Zheng Xuefeng Lu Qiufen Zhang Jian Zhang State Key Laboratory of Medical Genomics National Research Center for Translational Medicine at ShanghaiRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China Medicinal Chemistry and BioinformaticsCenter Shanghai Jiao Tong University School of MedicineShanghai 200025China Department of General Surgery Huashan HospitalCancer Metastasis InstituteFudan UniversityShanghai 200040China Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease School of Basic Medicine and Medical CenterUniversity of Science and Technology of ChinaHefei 230026China Department of Assisted Reproduction Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China College of Pharmacy Ningxia Medical UniversityYinchuan 750004China Department of Pancreatic Surgery Shanghai General HospitalShanghai Key Laboratory of Pancreatic DiseaseInstitute of Pancreatic DiseaseShanghai Jiao Tong University School of MedicineShanghai 200080China

Pancreatic cancer,one of the most aggressive malignancies,has no effective treatment due to the lack of targets and drugs related to tumour ***6 can promote the migration of pancreatic cancer and could be a potential target for antimetastasis of pancreatic ***,highly selective and potency SIRT6 inhibitor that can be used in vivo is yet to be ***,we developed a noveSIRT6 allosteric inhibitor,compound 11e,with maximal inhibitory potency and an IC_(50) value of 0.98±0.13μmol/***,compound 11e exhibited significant selectivity against other histone deacetylases(HADC1-11 and SIRT1-3)at concentrations up to 100μmol/*** allosteric site and the molecular mechanism of inhibition were extensively elucidated by cocrystal complex structure and dynamic structural ***,we confirmed the antimetastatic function of such inhibitors in four pancreatic cancer cell lines as well as in two mouse models of pancreatic cancer liver *** our knowledge,this is the first study to reveal the in vivo effects of SIRT6 inhibitors on liver metastatic pancreatic *** not only provides a promising lead compound for subsequent inhibitor developmentargeting SIRT6 but also provides a potential approach to address the challenge of metastasis in pancreatic cancer.

关键词： SIRT6 Inhibitor Allosteric Selectivity Cocrystal Pancreatic cancer metastasis

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承压水作用下岩质地层基坑抗突涌安全厚度研究

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建筑结构学报 2024年第6期45卷 213-223页

作者：王洪涛李建华殷允腾张红军宣兆腾赵明珠山东建筑大学土木工程学院山东济南250101 山东建筑大学建筑结构加固改造与地下空间工程教育部重点实验室山东济南250101 济南城建集团有限公司山东济南250031 山东省地质科学研究院山东济南250013

受下部承压水作用,基坑开挖后坑底发生突涌破坏是普遍存在的工程灾害。为保障基坑安全,设置足够的坑底厚度是阻止此类破坏的关键。基于塑性上限定理,将坑底突涌破坏范围分为上部被动区与下部剪切区两部分,分析了岩质地层中基坑突涌破坏... 详细信息

受下部承压水作用,基坑开挖后坑底发生突涌破坏是普遍存在的工程灾害。为保障基坑安全,设置足够的坑底厚度是阻止此类破坏的关键。基于塑性上限定理,将坑底突涌破坏范围分为上部被动区与下部剪切区两部分,分析了岩质地层中基坑突涌破坏机制。进一步,基于Hoek-Brown强度准则,推导了突涌破坏面曲线方程解析表达式,并给出了基坑临界安全厚度与承压水压力的理论预测公式。在此基础上,分析得到了被动区厚度、承压水压力、岩体强度参数等对基坑安全厚度及破坏范围的影响规律,并与现有研究方法、数值模拟得到的结果进行对比,验证了所提出方法的有效性。结果显示,基坑临界安全厚度与承压水压力、岩体经验参数等正相关,与被动区厚度、经验参数、岩体抗压强度、抗拉强度和重度等负相关。

关键词：基坑突涌承压水 Hoek-Brown强度准则上限定理安全厚度

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Emerging insights into heterotrimeric G protein signaling in plants

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Journal of Genetics and Genomics 2016年第8期43卷 495-502页

作者： Quan Xu mingzhu zhao Kun Wu Xiangdong Fu Qian Liu Rice Research Institute of Shenyang Agricultural University Key Laboratory of Northern Japonica Rice Genetics and BreedingMinistry of Education and Liaoning ProvinceKey Laboratory of Northeast Rice Biology and Genetics and BreedingMinistry of Agriculture The State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Heterotrimeric guanine nucleotide-binding protein （G protein） signaling is an evolutionarily conserved mechanism in diverse eukaryotic organisms. In plants, the repertoire of the heterotrimeric G protein complex, which is composed of the Ga, Gβ, and Gy subunits, is much simpler than that in metazoans, and the identity of typical G protein-coupled receptors （GPCRs） together with their ligands still remains unclear. Comparative phenotypic analysis in Arabidopsis and rice plants using gain- and loss-of-function mutants of G protein components revealed that heterotrimeric G protein signaling plays important roles in a wide variety of plant growth and developmental processes. Grain yield is a complex trait determined by quantitative trait loci （QTL） and is influenced by soil nitrogen availability and environmental changes. Recent studies have shown that the manipulation of two non-canonical Gy subunits, GS3 （GRAIN SIZE 3） and DEP1 （DENSE AND ERECT PANICLE 1）, represents new strategies to simultaneously increase grain yield and nitrogen use efficiency in rice. This review discusses the latest advances in our understanding of the heterotrimeric G protein signal transduction pathway and its application in improving yield and stress tolerance in crops.

关键词： G protein signalingNon-canonical G subunitsDEP1GS3Yield potential

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精细化手术室护理对宫腔镜下电切手术患者体温控制和应激反应的影响

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实用妇科内分泌电子杂志 2024年第3期11卷 150-152页

作者：张学娟王思思赵明珠东台市人民医院手术室江苏东台224200

目的探究精细化手术室护理对宫腔镜下电切手术患者体温控制和应激反应的影响。方法选取在本院开展宫腔镜下电切手术治疗的患者48例为研究对象,按照随机信封法将其分为两组,每组24例。对照组患者采用常规护理,观察组患者采用精细化手术... 详细信息

目的探究精细化手术室护理对宫腔镜下电切手术患者体温控制和应激反应的影响。方法选取在本院开展宫腔镜下电切手术治疗的患者48例为研究对象,按照随机信封法将其分为两组,每组24例。对照组患者采用常规护理,观察组患者采用精细化手术室护理。比较两组患者麻醉前、麻醉5min、手术15min与麻醉清醒后的体温水平,以及术中应激反应指标(心率、平均动脉压)。结果两组患者麻醉前、麻醉5min体温比较差异无统计学意义(P>0.05),观察组手术15min与麻醉清醒后体温高于对照组,差异有统计学意义(P<0.05)。观察组术中心率及平均动脉压低于对照组,差异有统计学意义(P<0.05)。结论精细化手术室护理应用在宫腔镜下电切手术患者护理中的效果显著,对患者体温控制与应激反应具有积极影响,可保证患者手术效果,值得临床推广与应用。

关键词：精细化手术室护理宫腔镜下电切手术体温控制应激反应

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Mechanism of allosteric activation of SIRT6 revealed by the action of rationally designed activators

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Acta Pharmaceutica Sinica B 2021年第5期11卷 1355-1361页

作者： Shaoyong Lu Yingyi Chen Jiacheng Wei mingzhu zhao Duan Ni Xinheng He Jian Zhang Department of Pathophysiology Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of EducationShanghai Jiao Tong UniversitySchool of MedicineShanghai 200025China State Key Laboratory of Oncogenes and Related Genes Renji HospitalShanghai Jiao Tong UniversitySchool of MedicineShanghai 200127China The Charles Perkins Centre University of SydneySydney NSW 2006Australia

The recent discovery of activator compounds binding to an allosteric site on the NAD+-dependent protein lysine deacetylase,sirtuin 6(SIRT6)has attracted interest and presents a pharmaceutical target for aging-related and cancer ***,the mechanism underlying allosteric activation of SIRT6 by the activator MDL-801 remains largely elusive because no major conformational changes are observed upon activator *** combining molecular dynamics simulations with biochemical and kinetic analyses of wild-type SIRT6 and its variant M136 A,we show that conformational rotation of 2-methyl-4-fluoro-5-bromo substituent on the right phenyl ring(R-ring)of MDL-801,which uncovers previously unseen hydrophobic interactions,contributes to increased activating deacetylation activity of *** hypothesis is further supported by the two newly synthesized MDL-801 derivatives through the removal of the 5-Br atom on the R-ring(MDL-801-D1)or the restraint of the rotation of the R-ring(MDL-801-D2).We further propose that the 5-Br atom serves as an allosteric driver that controls the ligand allosteric *** study highlights the effect of allosteric enzyme catalytic activity by activator binding and provides a rational approach for enhancing deacetylation activity.

关键词： Allosteric driver Allosteric sites Allosteric mechanisms Drug design Enzyme catalysis Protein dynamics

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Promoting or inhibiting? Green fiscal policy and urban green innovation

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Chinese Journal of Population,Resources and Environment 2023年第4期21卷 257-268页

作者： Jinrui Chen Zhuoji Zheng Xianfeng Han mingzhu zhao Mengfei Yin School of Business Administration Zhejiang Gongshang UniversityHangzhou 310018China School of Management and Economics Kunming University of Science and TechnologyKunming 650500China

Can green fiscal policy(GFP)incentivize the proliferation of urban green innovation(GI)and be a novel cata‐lyst for energy conservation and emission reduction within the“dual-carbon”framework?This paper explores GFP ramifications for GI by implementing a difference-in-differences model in a natural experiment centered on the“Comprehensive Demonstration City of Energy Saving and Emission Reduction Fiscal Policies”.The em‐pirical analysis reveals several key findings:(1)GFP exhibits significant augmentation at the GI level,with an observable evolutionary trend of increasing marginal ***,these outcomes withstand rigorous robustness tests,including propensity score matching.(2)A mechanism analysis elucidates the dual impact of GFP on GI *** directly fosters GI advancement indirectly by promoting talent aggregation,expanding scientific and technological investment,and attracting external financial resources.(3)A heterogeneity analy‐sis demonstrates that GFP enhancement of GI is closely associated with the patent category,manifesting a dis‐tinct pattern of“eastern region>other regions”and“non-resource cities>resource cities”.The study’s em‐pirical findings offer crucial real-world insights to guide the Chinese government in formulating a more effi‐cient GFP and facilitating the expansion of innovative endeavors while contributing to environmentally sus‐tainable and high-value development.

关键词： Green fiscal policy Green innovation Difference-in-differences modeling Dual-carbon goal Mechanism analysis

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Reorientation and obstacle avoidance control of free-floating modular robots using sinusoidal oscillator

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Chinese Journal of Aeronautics 2024年第6期37卷 262-275页

作者： Zhiyuan YANG mingzhu LAI Jian QI Ning zhao Xin SUI Jie zhao Yanhe ZHU State Key Laboratory of Robotics and System Harbin Institute of TechnologyHarbin 150001China School of Mathematics and Statistics Hainan Normal UniversityHaikou 571158China

This paper presents that a serpentine curve-based controller can solve locomotion control problems for articulated space robots with extensive flight phases,such as obstacle avoidance during free floating or attitude adjustment before *** proposed algorithm achieves articulated robots to use closed paths in the joint space to accomplish the above *** snakes,which can shuttle through gaps and adjust their landing posture by swinging their body during gliding in jungle environments,inspired the design of two *** first maneuver generates a rotation of the system by varying the moment of inertia between the joints of the robot,with the magnitude of the net rotation depending on the controller *** maneuver can be repeated to allow the robot to reach arbitrary *** second maneuver involves periodic undulations,allowing the robot to avoid collisions when the trajectory of the global Center of Mass(CM)passes through the *** maneuvers are based on the improved serpenoid curve,which can adapt to redundant systems consisting of different numbers of ***,the simulation illustrates that combining the two maneuvers can help a free-floating chain-type robot traverse complex *** proposed algorithm can be used with similar articulated robot models.

关键词： Collision avoidance Modularrobots Reorientation Free-floating space robots Nonholonomic systems Biologically inspired controllers Serpenoid curve

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“四责协同”机制构建医院行政科室权责清单

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现代医院 2024年第4期24卷 504-507页

作者：徐隽嬿金宇雅姜皓婧顾平赵明珠上海市浦东新区浦南医院上海200125

目的深化细化全面从严治党“四责协同”机制向科室延伸,构建医院行政职能科室监管事项权责一体化清单、压实责任。方法以行政职能科室岗位权力风险防控为基础,加强制度建设为抓手,监督权力运行为核心,对照法律法规、规章制度、岗位职责... 详细信息

目的深化细化全面从严治党“四责协同”机制向科室延伸,构建医院行政职能科室监管事项权责一体化清单、压实责任。方法以行政职能科室岗位权力风险防控为基础,加强制度建设为抓手,监督权力运行为核心,对照法律法规、规章制度、岗位职责,对重点领域、重要事项、关键岗位,查摆权力运行的关键节点、内部管理的薄弱环节、问题易发的风险点,采取自下而上与自上而下相结合的方式,形成行政科室权责一体化清单。结果医院17个行政科室以“一科室一清单”形式,共梳理出209项职权项目、涉及公权力947条。结论通过清单构建工作的开展,进一步完善了廉洁风险防控的制度建设,提升全院依法依纪依规工作的觉悟和能力和精细化管理水平,使党组织管党治党的责任得到有效落实,为医院高质量发展保驾护航。

关键词： “四责协同”机制医院行政权责清单

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