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Development and validation of a novel criterion of histologic healing in ulcerative colitis defined by inflammatory cell enumeration in lamina propria mucosa: A multicenter retrospective cohort in China

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中华医学杂志(英文版) 2024年第11期137卷 2024 May 13页

作者： Gao Han Peng Kangsheng Shi Yadi zhu Shenshen Sun Ruicong Xu Chunjin Liu Ping Pang Zhi zhu Lanxiang Chen Weichang Feng Baisui Wu Huili Zhou Guangxi Li mingsong Li Junxiang Ding Baijing Liu Zhanju Department of Gastroenterology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou Guangdong China Department of Gastroenterology Dongfang Hospital of Beijing University of Chinese Medicine Beijing China Center for IBD Research and Department of Gastroenterology Shanghai Tenth People’s Hospital Tongji University School of Medicine Shanghai China Clinical Medicine Sanquan College of Xinxiang Medical University Xinxiang Henan China Department of Gastroenterology The First People’s Hospital of Shangqiu City Affiliated to Xinxiang Medical University Shangqiu Henan China Department of Gastroenterology Wuhu First People’s Hospital Wuhu Anhui China Department of Gastroenterology Suzhou Municipal Hospital Affiliated to Nanjing Medical University Suzhou Jiangsu China Department of Gastroenterology The First Affiliated Hospital of Soochow University Suzhou Jiangsu China Department of Gastroenterology The Second Affiliated Hospital of Zhengzhou University Zhengzhou Henan China Department of Gastroenterology Zhengzhou Central Hospital Affiliated to Zhengzhou University Zhengzhou Henan China Department of Gastroenterology The Affiliated Hospital of Jining Medical College Jining Shandong China

Background: Histological healing is closely associated with improved long-term clinical outcomes and lowered relapses in patients with ulcerative colitis (UC). Here, we developed a novel diagnostic criterion for assessing histological healing in UC patients.Methods: We conducted a retrospective cohort study in UC patients, whose treatment was iteratively optimized to achieve mucosal healing at Shanghai Tenth People’s Hospital of Tongji University from January 2017 to May 2022. We identified an inflammatory cell enumeration index (ICEI) for assessing histological healing based on the proportions of eosinophils, CD177+ neutrophils, and CD40L+ T cells in the colonic lamina propria under high power field (HPF), and the outcomes (risks of symptomatic relapses) of achieving histological remissionvs. persistent histological inflammation using Kaplan-Meier curves. Intrareader reliability and inter-reader reliability were evaluated by each reader. The relationships to the changes in the Nancy index and the Geboes score were also assessed for responsiveness. The ICEI was further validated in a new cohort of UC patients from other nine university hospitals.Results: We developed an ICEI for clinical diagnosis of histological healing, i.e., Y = 1.701X1 + 0.758X2 + 1.347X3 - 7.745 (X1, X2, and X3 represent the proportions of CD177+ neutrophils, eosinophils, and CD40L+ T cells, respectively, in the colonic lamina propria under HPF). The receiver operating characteristics curve (ROC) analysis revealed that Y <-0.391 was the cutoff value for the diagnosis of histological healing and that an area under the curve (AUC) was 0.942 (95% confidence interval [CI]: 0.905-0.979) with a sensitivity of 92.5% and a specificity of 83.6% (P <0.001). The intraclass correlation coefficient (ICC) for the intrareader reliability was 0.855 (95% CI: 0.781-0.909), and ICEI had good inter-reader reliability of 0.832 (95% CI: 0.748-0.894). During an 18-month follow-up, patients with histological healing had a substantially better outcome compared with

关键词： Ulcerative colitis Histological healing Inflammatory cell enumeration index Prognosis

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The Dof transcription factor COG1 acts as a key regulator of plant biomass by promoting photosynthesis and starch accumulation

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Molecular Plant 2023年第11期16卷 1759-1772页

作者： zhuoyun Wei Haoyong Zhang Meng Fang Shuyuan Lin mingsong zhu Yuxiu Li Limin Jiang Tianliang Cui Yanwei Cui Hong Kui Liang Peng Xiaoping Gou Jia Li Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou UniversityLanzhou 730000China Gansu Key Laboratory of Gene Editing for Breeding School of Life SciencesLanzhou UniversityLanzhou 730000China Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution School of Life SciencesLanzhou UniversityLanzhou 730000China Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design School of Life SciencesGuangzhou UniversityGuangzhou 510006China

Photosynthetic efficiency is the primary determinant of crop yield,including vegetative biomass and grain yield.Manipulation of key transcription factors known to directly control photosynthetic machinery can be an effective strategy to improve photosynthetic traits.In this study,we identified an Arabidopsis gain-of-function mutant,cogwheel1-3D,that shows a significantly enlarged rosette and increased biomass compared with wild-type plants.Overexpression of COG1,a Dof transcription factor,recapitulated the phenotype of cogwheel1-3D,whereas knocking out COG1 and its six paralogs resulted in a reduced rosette size and decreased biomass.Transcriptomic and quantitative reverse transcription polymerase chain reaction analyses demonstrated that COG1 and its paralogs were required for light-induced expression of genes involved in photosynthesis.Further chromatin immunoprecipitation and electrophoretic mobility shift assays indicated that COG1 can directly bind to the promoter regions of multiple genes encoding light-harvesting antenna proteins.Physiological,biochemical,and microscopy analyses revealed that COG1 enhances photosynthetic capacity and starch accumulation in Arabidopsis rosette leaves.Furthermore,combined results of bioinformatic,genetic,and molecular experiments suggested that the functions of COG1 in increasing biomass are conserved in different plant species.These results collectively demonstrated that COG1 acts as a key regulator of plant biomass by promoting photosynthesis and starch accumulation.Manipulating COG1 to optimize photosynthetic capacity would create new strategies for future crop yield improvement.

关键词： COG1 Dof transcription factor LHCA LHCB photosynthetic genes biomass

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Conserved and differentiated functions of CIK receptor kinases in modulating stem cell signaling in Arabidopsis

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Molecular Plant 2021年第7期14卷 1119-1134页

作者： Yafen zhu Chong Hu Yanwei Cui Li Zeng Sunjingnan Li mingsong zhu Fanhui Meng Shuting Huang Li Long Jing Yi Jia Li Xiaoping Gou Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou UniversityLanzhou 730000China

The shoot apical meristem(SAM)and root apical meristem(RAM)act as pools of stem cells that give rise to aboveground and underground tissues and organs in higher plants,respectively.The CLAVATA3(CLV3)-WUSCHEL(WUS)negative-feedback loop acts as a core pathway controlling SAM homeostasis,while CLV3/EMBRYO SURROUNDING REGION(ESR)40(CLE40)and WUSCHEL-RELATED HOMEOBOX5(WOX5),homologs of CLV3 and WUS,direct columella stem cell fate.Moreover,CLV3 INSENSITIVE KINASES(CIKs)have been shown to be essential for maintaining SAM homeostasis,whereas whether they regulate the distal root meristem remains to be elucidated.Here,we report that CIKs are indispensable for transducing the CLE40 signal to maintain homeostasis of the distal root meristem.We found that the cik mutant roots displayed disrupted quiescent center and delayed columella stem cell(CSC)differentiation.Biochemical assays demonstrated that CIKs interact with ARABIDOPSIS CRINKLY4(ACR4)in a ligand-independent manner and can be phosphorylated by ACR4 in vitro.In addition,the phosphorylation of CIKs can be rapidly induced by CLE40,which partially depends on ACR4.Although CIKs act as conserved and redundant regulators in the SAM and RAM,our results demonstrated that they exhibit differentiated functions in these meristems.

关键词： ACR4 CIKs CLE40 stem cells distal root meristem phosphorylation

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SERK Receptor-like Kinases Control Division Patterns of Vascular Precursors and Ground Tissue Stem Cells during Embryo Development in Arabidopsis

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Molecular Plant 2019年第7期12卷 984-1002页

作者： Huiqiang Li Zeping Cai Xiaojuan Wang Meizhen Li Yanwei Cui Nan Cui Fei Yang mingsong zhu Junxiang Zhao Wenbin Du Kai He Jing Yi Frans E.Tax Suiwen Hou Jia Li Xiaoping Gou Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life Sciences Lanzhou University Lanzhou Gansu 730000 China College of Forestry Hainan University Danzhou Hainan 571737 China Department of Molecular and Cellular Biology University of Arizona Tucson AZ 85721 USA

During embryo development, the vascular precursors and ground tissue stem cells divide to renew them-selves and produce the vascular tissue, endodermal cells, and cortical cells. However, the molecular mech-anisms regulating division of these stem cells have remained largely elusive. In this study, we show that loss of function of SOMATIC EMBRYOGENESIS RECEPTOR-UKE KINASE (SERK) genes results in aberrant em-bryo development. Fewer cortical, endodermal, and vascular cells are generated in the embryos of serk1 serk2bak1 triple mutants. WUSCHEL-RELATED HOMBOBOX5 (WOXS) is ectopically expressed in vascular cells of serkl serk2 bak1 embryos. The first transverse division of vascular precursors in mid-globular em-bryos and second asymmetric division of ground tissue stem cells in early-heart embryos are abnormally altered to a longitudinal division. The embryo defects can be partially rescued by constitutively activated mitogen-activated protein kinase (MAPK) kinase kinase YODA (YDA) and MAPK kinase MKK5. Taken together, our results reveal that SERK-mediated signals regulate division patterns of vascular precursors and ground tissue stem cells, likely via the YDA-MKK4/5 cascade, during embryo development.

关键词： embryo ground tissue stem cell MKK4 MKK5 SERKs vascular precursors

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CT影像组学预测结肠癌淋巴血管间隙浸润价值研究

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中国医学计算机成像杂志 2024年第2期30卷 211-217页

作者：朱先锋张虎张晓金吴树剑董明松许汉明吴宣芜湖市第二人民医院影像科芜湖241000 皖南医学院第一附属医院(弋矶山医院)影像科

目的:构建预测结肠癌淋巴血管间隙浸润(LVSI)的临床影像模型、CT影像组学模型和两者联合模型,并从中筛选出最优模型。方法:收集接受增强CT检查的365例结肠癌患者资料,根据手术病理结果分为LVSI+(n=84)及LVSI-(n=281),样本按7:3比例随机... 详细信息

目的:构建预测结肠癌淋巴血管间隙浸润(LVSI)的临床影像模型、CT影像组学模型和两者联合模型,并从中筛选出最优模型。方法:收集接受增强CT检查的365例结肠癌患者资料,根据手术病理结果分为LVSI+(n=84)及LVSI-(n=281),样本按7:3比例随机分为训练集(n=256)和验证集(n=109)。比较临床、影像指标组间差异并建立临床影像模型;对静脉期CT图像上全肿瘤区域进行感兴趣区(ROI)勾画并提取影像组学特征,经降维、筛选后共获得16个最优特征并建立CT影像组学模型;再构建临床影像与CT影像组学联合模型。比较各模型效能,绘制最优模型列线图,并评估其性能。结果:LVSI+及LVSI-组间淋巴结长径、癌胚抗原(CEA)、糖类抗原19-9(CA19-9)、肿瘤部位差异均有统计学意义(P<0.05)。临床影像模型、CT影像组学及联合模型受试者工作特征曲线下面积(AUC)分别为0.726、0.784、0.851(训练集)和0.729、0.768、0.833(验证集)。联合模型AUC均大于临床影像模型(P<0.05)。列线图校准度较高,决策曲线范围为0.15~0.98时临床有获益。结论:基于临床影像指标及CT影像组学构建的列线图预测结肠癌患者LVSI的价值较高。

关键词：结肠肿瘤计算机体层成像影像组学淋巴血管间隙浸润

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六味地黄丸对钛颗粒诱导骨溶解成骨影响的机制

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中国组织工程研究 2024年第3期28卷 392-397页

作者：朱志祺苑思杰张梓琳纪仕杰蒙明松颜安明韩婧南京中医药大学药学院江苏省南京市210032 解放军32251部队门诊部福建省厦门市361007

背景:目前已有大量研究发现六味地黄丸可用来治疗骨质疏松,而鲜有关于六味地黄丸对磨损微粒诱导的成骨细胞分化与矿化方面的相关研究。目的:探讨不同浓度六味地黄丸含药血清对钛颗粒诱导的小鼠MC3T3-E1成骨细胞体外骨溶解模型的积极作... 详细信息

背景:目前已有大量研究发现六味地黄丸可用来治疗骨质疏松,而鲜有关于六味地黄丸对磨损微粒诱导的成骨细胞分化与矿化方面的相关研究。目的:探讨不同浓度六味地黄丸含药血清对钛颗粒诱导的小鼠MC3T3-E1成骨细胞体外骨溶解模型的积极作用。方法:大鼠口服六味地黄丸后提取含药血清,筛选六味地黄丸含药血清和钛颗粒对MC3T3-E1细胞活力影响的最佳浓度。将MC3T3-E1细胞为3组:空白组给予成骨分化培养;模型组给予钛颗粒(5μg/mL)+成骨分化培养;加含药血清组给予钛颗粒(5μg/mL)+六味地黄丸含药血清(分为10%,15%,20%剂量)共培养+成骨诱导。通过CCK-8法检测成骨细胞活力,碱性磷酸酶染色检测细胞碱性磷酸酶活性,通过硝酸银(Von Kossa)和茜素红染色检测细胞矿化情况,qRT-PCR检测成骨分化相关基因Runx-2、Osterix、Ocn、Axin、ALP和Opn的表达,Western blot检测Wnt/β-catenin信号通路β-catenin、p-GSK-3β、GSK-3β、Runx2和Osterix蛋白表达。结果与结论:①六味地黄丸含药血清培养可以逆转钛颗粒诱导的MC3T3E-1细胞碱性磷酸酶活性的降低,增加MC3T3E-1细胞茜素红染色及钙化结节,增加MC3T3E-1细胞成骨相关基因的表达,同时提高Wnt/β-catenin信号通路相关蛋白的表达。②结果说明,六味地黄丸含药血清体外可以逆转钛颗粒对成骨细胞分化矿化的抑制作用,上调成骨相关基因的表达,其机制与调控Wnt/β-catenin信号通路相关,提示六味地黄丸有望成为防治人工关节无菌性松动的有效药物。

关键词：六味地黄丸含药血清成骨细胞骨溶解 Wnt/β-catenin 钛颗粒

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^(18)F-FDG PET/CT代谢特征联合HRCT影像特征在鉴别周围型肺癌与炎性病变中的价值

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标记免疫分析与临床 2024年第4期31卷 721-726页

作者：姜春利朱峰岭陶秀霞董明松芜湖市第二人民医院核医学科安徽芜湖241000 芜湖市第二人民医院医学影像科安徽芜湖241000

目的评估18氟-脱氧葡萄糖(^(18)F-FDG)正电子发射型计算机断层显像(PET/CT)代谢特征联合高分辨率CT(HRCT)影像特征在鉴别周围型肺癌(PLC)与炎性病变中的价值。方法回顾性分析2015年3月至2023年5月期间行^(18)F-FDG PET/CT检查并经病理... 详细信息

目的评估18氟-脱氧葡萄糖(^(18)F-FDG)正电子发射型计算机断层显像(PET/CT)代谢特征联合高分辨率CT(HRCT)影像特征在鉴别周围型肺癌(PLC)与炎性病变中的价值。方法回顾性分析2015年3月至2023年5月期间行^(18)F-FDG PET/CT检查并经病理证实或临床随访诊断的肺占位性病变91例,其中PLC组61例,炎性病变组30例。对两组病灶的HRCT影像特征及^(18)F-FDG代谢特征进行单因素、多因素分析,比较两组间各项特征的差异,并使用ROC曲线下面积AUC评价HRCT影像特征法、^(18)F-FDG代谢特征法以及联合诊断的诊断效能,运用Delong检验比较3者之间诊断效能差异。结果单因素分析显示两组病灶直径、空气支气管征、卫星征、全部摄取及最大标准化摄取值(SUVmax)差异有统计学意义(P0.05)。多因素分析显示病灶直径、空气支气管征、卫星征及SUVmax对鉴别周围型肺癌与炎性病变有价值(P0.05)。通过ROC曲线分析显示,联合诊断的AUC最大,Delong检验结果显示其诊断效能分别优于HRCT影像特征法、^(18)F-FDG代谢特征法(P<0.05)。结论^(18)F-FDG PET/CT代谢特征联合HRCT影像特征在鉴别周围型肺癌与炎性病变中具有较高的临床价值。

关键词：正电子发射计算机断层显像代谢特征高分辨率CT 周围型肺癌炎性病变

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PET示踪剂在阿尔茨海默病早期诊断中的研究进展

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河北科技大学学报 2024年第2期45卷 181-188页

作者：刘文清刘明松杨莎马文杰朱华结徐志栋河北科技大学化学与制药工程学院河北石家庄050018 河北省药用分子重点实验室河北石家庄050018

阿尔茨海默病(Alzheimer′s disease,AD)是一种发病机理极其复杂的神经退行性疾病,以认知功能障碍和记忆丧失为特征,涉及多种生理、病理过程。早期诊断是AD预防和治疗的关键。正电子发射断层成像(PET)技术能够在分子层面准确检测神经元... 详细信息

阿尔茨海默病(Alzheimer′s disease,AD)是一种发病机理极其复杂的神经退行性疾病,以认知功能障碍和记忆丧失为特征,涉及多种生理、病理过程。早期诊断是AD预防和治疗的关键。正电子发射断层成像(PET)技术能够在分子层面准确检测神经元与脑组织中细微的生理变化,为AD早期诊断提供依据。从生物标志物的分类入手,介绍了脑中蛋白变性与神经元损伤的相关靶点,根据亲和力、半衰期、结合区域、药代动力学、分析方法以及结果相关性等几个重要参数,综述了各类PET示踪剂的研究现状,指出目前PET示踪剂开发面临的问题,认为未来研究应从以下几个方面展开:神经炎症相关PET示踪剂的开发;已有示踪剂的进一步评估和优化;示踪剂结果分析方法的规范化和标准化。

关键词：医学影像学正电子发射断层成像技术 PET示踪剂阿尔茨海默病临床诊断

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CT影像组学对Ⅱ期结肠癌风险分层的预测价值探讨

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现代肿瘤医学 2024年第1期32卷 120-125页

作者：张晓金张虎吴树剑徐家军黄国权董明松朱先锋王家伟芜湖市第二人民医院医学影像科安徽芜湖241000 皖南医学院第一附属医院放射科安徽芜湖241000 芜湖市第二人民医院泌尿外科安徽芜湖241000

目的:探讨CT影像组学模型对Ⅱ期结肠癌(colon cancer,CC)风险分层的预测价值。方法:连续搜集2015年01月至2023年07月经手术证实的167例Ⅱ期CC患者资料,按欧洲肿瘤内科学会(European Society of Medical Oncology,ESMO)指南将患者分为低... 详细信息

目的:探讨CT影像组学模型对Ⅱ期结肠癌(colon cancer,CC)风险分层的预测价值。方法:连续搜集2015年01月至2023年07月经手术证实的167例Ⅱ期CC患者资料,按欧洲肿瘤内科学会(European Society of Medical Oncology,ESMO)指南将患者分为低风险组77例,中高风险组90例;按7∶3将样本随机分为训练组(n=116)与内部验证组(n=51)。在术前静脉期CT图像上对肿瘤进行分割并提取影像组学特征,特征经降维、筛选后,采用logistic回归分析构建预测模型,观察模型校准度和临床获益。结果:训练组中高风险64/116例;内部验证组中高风险26/51例,两组间观测指标差异无统计学意义(均P>0.05)。共筛选出6个影像组学特征用于构建Ⅱ期CC低风险和中高风险的预测模型,训练组AUC为0.822,灵敏度:84.4%,特异度:71.2%;内部验证组AUC为0.802,灵敏度:96.2%,特异度:60.0%。模型显示出较高的校准度;决策曲线分析(decision curve analysis,DCA)阈值概率范围0.04~0.85时临床有获益。结论:基于静脉期CT图像构建的影像组学模型实现了低风险和中高风险Ⅱ期CC的准确分层,有望改善临床对该类患者的分层管理。

关键词：影像组学 Ⅱ期结肠癌风险分层

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试析曹寅对扬州地方文化的构建

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曹雪芹研究 2023年第4期 39-48页

作者：朱明松扬州市世界遗产保护管理办公室扬州大学苏中发展研究院

作为钦点巡视两淮、兼管巡盐御史,曹寅在扬州为政多年,在扬州开展较多的文化活动。本文从侍君以忠、恤商以仁、施政以勤、尊贤以礼、纪事以诗、刻书以专等六个方面,揭示他对扬州地域文化构建的重要贡献。

关键词：曹寅扬州地方文化

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