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Reproductive toxicity study with a novel deoxyguanosine analogue （Metacavir） in pregnant SD rats

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Frontiers of Medicine 2015年第1期9卷 82-89页

作者： Qihui Luo Zhengli Chen Anchun Cheng mingshu wang Jing Fang Xi Peng Li Tang Key Laboratory of Animal Disease and Human Health of Sichuan Province Ya 'an 625014 China Institute of Preventive Veterinary Medicine Sichuan Agricultural University Chengdu 611130 China

Our preliminary studies demonstrated that Metacavir has potential to become a new anti-HBV agent. The main targets of the toxic effects of Metacavir, in rhesus monkeys, were gastrointestinal tracts, liver, blood, and kidneys, which were not related to mitochondrial effects. In this study, the maternal toxicity, embryo-fetal developmental toxicity and teratogenicity were studied in pregnant Sprague-Dawley rats after intragastric administration of Metacavir （200, 100, 50, 0 mg/kg body weight） during the first 6-15 days of pregnancy. Slower weight gain was observed in 5 out of 21 rats subjected to a 200 mg/kg dose, as well as 2 out of 20 subjected to a 100 mg/kg dose. Compared with the solvent control group, the calibration weight gain in the 200 mg/kg and 100 mg/kg dosage groups respectively, during first 6-20 pregnant days were significantly different （P 〈 0.01, P 〈 0.05）. Significant dose related adverse effects to other reproductive parameters were not seen in F0 and F1, but the number of stillbirths in high dose group showed notably difference compared with the control group （P 〈 0.05）, while the litter incidence showed no difference. No Metacavir-associated pathological changes were observed. The present research indicated that at a dose of 200 mg/（kg·d）（i.e., 40 times the effective dose in rats）, Metacavir shows some maternal toxicity to SD rats. The embryotoxicity in the 200 mg/kg group encompass decreased fetal body weight, and higher fetal mortality rates, compared with the control group. However, the litter incidence showed no statistical difference. All the treated rats displayed normal bone development, no teratogenicity and without adverse effects on fetal development, thus indicating that below a dose of 200 mg/（kg· d） there is no teratogenic side effects.

关键词： deoxyguanosine analogue Metacavir pregnancy maternal toxicity embryo toxicity teratogenicity

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利用云模型评价开发区的土地集约利用状况

农业工程学报 2012年第10期28卷 247-252页

作者：王明舒朱明南京大学地理与海洋科学学院南京210093

针对当前开发区土地集约评价中权重确定方法受主观性影响较大的问题,该文提出基于云模型的权重确定方法。将云模型引入开发区土地集约利用评价,并以桂林国家级高新技术产业开发区为例,进行实证研究。研究结果表明桂林高新区土地利用集... 详细信息

针对当前开发区土地集约评价中权重确定方法受主观性影响较大的问题,该文提出基于云模型的权重确定方法。将云模型引入开发区土地集约利用评价,并以桂林国家级高新技术产业开发区为例,进行实证研究。研究结果表明桂林高新区土地利用集约度综合分值为0.889,用地供需基本平衡;从土地利用状况而言,其土地利用强度较高、工业用地水平较强;就用地效益而言,工业用地产出强度较好,高新技术产业用地产出强度较高;从管理绩效来看,截止评价时点,区内无闲置土地,监管成效显著。基于云模型确定权重值的方法具备结果更加客观,算法容易实现的优点,有助于促进土地集约利用的科学评价,为桂林高新区土地集约利用政策依据。

关键词：土地利用模型指标土地集约利用云模型开发区桂林

Construction of an Infectious Clone for Mosquito-Derived Tembusu Virus Prototypical Strain

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Virologica Sinica 2021年第6期36卷 1678-1681页

作者： Xiaoli wang Yu He Jiaqi Guo Bowen Jiang mingshu wang Renyong Jia Dekang Zhu Mafeng Liu Xinxin Zhao Qiao Yang Ying Wu Shaqiu Zhang Yunya Liu Ling Zhang Yanling Yu Anchun Cheng Shun Chen Research Center of Avian Disease College of Veterinary MedicineSichuan Agricultural UniversityChengdu 611130China Institute of Preventive Veterinary Medicine College of Veterinary MedicineSichuan Agricultural UniversityChengdu 611130China Key Laboratory of Animal Disease and Human Health of Sichuan Province Chengdu 611130China

Dear Editor,Duck Tembusu virus(DTMUV),a member of the Flavivirus genus within the Flaviviridae family,has caused huge economic losses to the poultry industry in China and even in Asia since 2010(Zhang et al.2017).The first strain of Tembusu virus(TMUV)MM_1775 was isolated from mosquitoes in 1955 in Malaysia(Platt et al.1975).DTMUV CQW1 strain(GenBank:KM233707.1)was isolated from the liver tissue of Cherry Valley ducks in southwest China in 2015(Zhu et al.2015).The positive control rCQW1 was rescued from an infectious clone that contained the full-length cDNA of *** the complete cDNA was positioned under the control of the T7 promoter elements for in vitro transcription(Chen et al.2018).The relationship between TMUV evolution and pathogenic variants has not been *** fundamental reason is the lack of the in vitro operation platform for the prototypical strain genome.

关键词： poultry Infectious Clone

Comparative study of the pathogenicity of the mosquito origin strain and duck origin strain of Tembusu virus in ducklings and three-week-old mice

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Virologica Sinica 2023年第5期38卷 827-831页

作者： Xiaoli wang Yu He Jiaqi Guo Zhen Wu Andres Merits mingshu wang Renyong Jia Dekang Zhu Mafeng Liu Xinxin Zhao Qiao Yang Ying Wu Shaqiu Zhang Juan Huang Xumin Ou Qun Gao Di Sun Anchun Cheng Shun Chen Institute of Preventive Veterinary Medicine Sichuan Agricultural UniversityChengdu611130China Research Center of Avian Disease College of Veterinary MedicineSichuan Agricultural UniversityChengdu611130China Key Laboratory of Animal Disease and Human Health of Sichuan Province Sichuan Agricultural UniversityChengdu611130China Institute of Technology University of TartuTartu999148Estonia

Dear Editor,Duck Tembusu virus(DTMUV)is the causative agent of a new,acute and severe infectious disease in ducks(Su et al.,2011).TMUV was first isolated from Culex tritaeniorhynchus in Malaysia in 1955(Platt et al.,1975),and this strain is regarded as a reference *** our previous study,an infectious clone for the mosquito-derived Tembusu virus prototypical strain MM_1775(GenBank:JX477685.2)was constructed,and the rMM_1775 virus was rescued successfully(wang et al.,2021).And the TMUV strain rCQW1 was rescued by a reverse genetic system using the viral RNAs of CQW1(GenBank:KM233707.1)(Chen et al.,2018;Guo et al.,2020),which was isolated from ducks in 2013(Zhu et al.,2015).

关键词： duck strain origin

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革兰氏阴性菌膜间质蛋白酶DegP研究进展

微生物学报 2019年第7期59卷 1211-1221页

作者：王昌宇汪铭书程安春四川农业大学动物医学院禽病防治研究中心四川成都611130 四川农业大学动物医学院预防兽医研究所四川成都611130 动物疫病与人类健康四川省重点实验室四川成都611130

膜间质蛋白酶(DegP),是一种广泛存在于真核生物和原核生物细胞中的蛋白。DegP同时具有酶活性和分子伴侣活性,并通过多聚体构成胶囊状结构执行其分子伴侣功能。DegP的酶活性依赖酶切位点与PDZ1结构域双重识别方式识别底物,这种识别模式... 详细信息

膜间质蛋白酶(DegP),是一种广泛存在于真核生物和原核生物细胞中的蛋白。DegP同时具有酶活性和分子伴侣活性,并通过多聚体构成胶囊状结构执行其分子伴侣功能。DegP的酶活性依赖酶切位点与PDZ1结构域双重识别方式识别底物,这种识别模式被称为"分子量尺"。在革兰氏阴性菌中,DegP主要位于膜间质,通过分子伴侣活性与酶活性帮助保护错误折叠蛋白或降解变性蛋白。DegP也参与外膜蛋白的转运,是DegP胞内活性的研究重点。DegP也可以被分泌到胞外,帮助宿主对抗恶劣环境,并参与调节生物被膜的形成。本文将从DegP的结构与活性、胞内功能与胞外功能三大方面对DegP的研究进展进行总结,为革兰氏阴性菌周质中蛋白质质量控制与DegP体外功能的进一步研究提供参考。

关键词：革兰氏阴性菌 DegP 分子伴侣蛋白酶蛋白质质量控制系统

Dynamic changes of apoptosis in duck embryo fibroblasts induced by new type Gosling viral enteritis virus

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Progress in Natural Science:Materials International 2008年第2期18卷 239-244页

作者： Shun Chen a,Anchun Cheng a,b,,mingshu wang a,b,Xiaoyue Chen a a Avian Disease Research Center,College of Veterinary Medicine of Sichuan Agricultural University,Yaan 625014,China b Key Laboratory of Animal Disease and Human Health of Sichuan Province,Yaan 625014,China Avian Disease Research Center College of Veterinary Medicine of Sichuan Agricultural University Yaan 625014 China Key Laboratory of Animal Disease and Human Health of Sichuan Province Yaan 625014 China

The monolayer duck embryo fibroblast (DEF) cells were experimentally infected with new type Gosling viral enteritis virus (NGVEV) and the dynamic changes of apoptosis were detected at different time points after NGVEV infection by transmission electron microscopy (TEM),DNA agarose gel electrophoresis and Annexin V-FITC/PI stained fluorescence-activated cell sorter (FACS). The result shows that NGVEV can induce infected cells undergoing apoptosis and changing regularly. A series of characteristic apoptotic morphological changes including shrinkage of the cells,chromatin condensation and margination,as well as formation of apoptotic bodies,were observed by TEM. The typical ladder pattern of DNA fragmentation was demonstrated by agarose gel electrophoresis. And using flow cytometry analysis of Annexin V-FITC/PI staining,the dead,viable,apoptotic and necrotic cells could be analyzed quantitatively.

关键词： New type Gosling viral enteritis virus (NGVEV) Duck embryo fibroblasts (DEF) Apoptosis

Emergence of a novel multi-resistance-mediating integrative and conjugative element ICEPmu3 in Pasteurella multocida

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Journal of Integrative Agriculture 2024年第11期23卷 3938-3942页

作者： Jiao He Zhishuang Yang mingshu wang Renyong Jia Shun Chen Mafeng Liu Xinxin Zhao Qiao Yang Ying Wu Shaqiu Zhang Juan Huang Xumin Ou Di Sun Bin Tian Yu He Zhen Wu Anchun Cheng Dekang Zhu Research Center of Avian Diseases College of Veterinary MedicineSichuan Agricultural UniversityChengdu 611130China Key Laboratory of Agricultural Bioinformatics Ministry of EducationChengdu 611100China International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province Chengdu 610199China Engineering Research Center of Southwest Animal Disease Prevention and Control Technology Ministry of EducationChengdu 610199China

Pasteurella multocida(PM)has been infecting a variety of hosts for a long time,causing sustained economic losses worldwide;however,there have been limited studies on its extensive adaptability(Aktories et al.2012).Analysis of strains data collected in our laboratory revealed that PM typically acquires foreign genes through transformation and conjugation,rather than transformation and *** and conjugative elements(ICEs)are a crucial mechanism that leads to abrupt changes in niche preferences and enhances environmental adaptability for bacteria,with their number far exceeding the number of plasmids and phages(Wozniak and Waldor 2010;Johnson and Grossman 2015;Botelho and Schulenburg 2021).Previous research has shown that the European bovine-sourced strain Pm36950 contains the experimentally transferable resistant ICEPmu1,which could integrate into the host chromosome or forming a circular intermediate(Michael et al.2012).Additionally,a few studies have suggested that PM strains isolated from European bovine and Asian swine might contain ICEs,but these claims lack experimental verification(Klima et al.2014;Moustafa et al.2015;Kadlec et al.2017;Peng et al.2017;Beker et al.2018;Schink et al.2022).Currently,there is no data available on ICE-carrying PM strains isolated from hosts outside of European cattle or Chinses *** letter presents a report on a novel ICE identified in the hypervirulent and multidrug-resistant PM HN141014 strain isolated from Chinese *** ICE was specifically analyzed for its resistance genes,transferable capacity and host diversity.

关键词： conjugative element exceeding

中医典籍《难经》英译本在海外的传播与接受研究

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外国语文 2023年第4期39卷 43-51页

作者：王明树王格四川外国语大学翻译学院重庆400031

中医药典籍是中国传统医学文化的重要瑰宝之一,其翻译工作以及海外传播对于中国文化“走出去”具有重大意义。本文以中医药典籍《难经》作为研究对象,首先梳理其成书、通行本以及英译本情况,再从全球图书馆馆藏情况、两大书评网站(Amazo... 详细信息

中医药典籍是中国传统医学文化的重要瑰宝之一,其翻译工作以及海外传播对于中国文化“走出去”具有重大意义。本文以中医药典籍《难经》作为研究对象,首先梳理其成书、通行本以及英译本情况,再从全球图书馆馆藏情况、两大书评网站(Amazon和Goodreads)中的书籍销售情况、海外读者反馈(普通读者评论以及专业读者的书评和引用情况)三个方面对其英译本的海外传播和接受情况进行考察,以期对中医文化的对外传播具有一定的参考价值。

关键词：中医典籍英译《难经》海外传播与接受

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概念隐喻视角下翻译原则、翻译策略或方法选取背后的认知机制

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外国语文 2017年第2期33卷 99-103页

作者：王明树四川外国语大学国际关系学院重庆400031

本文以概念隐喻理论为框架,首先探讨"翻译隐喻"和"译者身份隐喻"的定义以及"翻译隐喻"之间的映射过程,然后考察"译者身份隐喻"与翻译原则、翻译策略或方法选取之间的关系及其背后的认知规律。"译者身份隐喻"与翻译原则、翻译策略或方法... 详细信息

本文以概念隐喻理论为框架,首先探讨"翻译隐喻"和"译者身份隐喻"的定义以及"翻译隐喻"之间的映射过程,然后考察"译者身份隐喻"与翻译原则、翻译策略或方法选取之间的关系及其背后的认知规律。"译者身份隐喻"与翻译原则、翻译策略或方法的选取之间的关系不是任意的,而是有其特定的认知规律。

关键词：概念隐喻 “翻译隐喻” “译者身份隐喻” 认知机制