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Genetic and pathogenic characterization of new infectious bronchitis virus strains in the GVI-1 and GI-19 lineages isolated in central China

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Journal of Integrative Agriculture 2024年第7期23卷 2407-2420页

作者： Yuhan Yang Dou Wang Yaning Bai Wenyan huang Shimin Gao Xingchen Wu Ying Wang Jianle Ren Jinxin He Lin Jin mingming hu Zhiwei Wang Zhongbing Wang Haili Ma Junping Li Libin Liang College of Veterinary Medicine Shanxi Agricultural UniversityJinzhong 030801China Shanxi Provincial Animal Disease Prevention and Control Center Taiyuan 030000China

Avian infectious bronchitis(IB)is a highly contagious infectious disease caused by infectious bronchitis virus(IBV),which is prevalent in many countries worldwide and causes serious harm to the poultry industry.At present,many commercial IBV vaccines have been used for the prevention and control of IB;however,IB outbreaks occur frequently.In this study,two new strains of IBV,SX/2106 and SX/2204,were isolated from two flocks which were immunized with IBV H120 vaccine in central China.Phylogenetic and recombination analysis indicated that SX/2106,which was clustered into the GI-19 lineage,may be derived from recombination events of the GI-19 and GI-7 strains and the LDT3-A vaccine.Genetic analysis showed that SX/2204 belongs to the GVI-1 lineage,which may have originated from the recombination of the GI-13 and GVI-1 strains and the H120 vaccine.The virus cross-neutralization test showed that the antigenicity of SX/2106 and SX/2204 was different from H120.Animal experiments found that both SX/2106 and SX/2204 could replicate effectively in the lungs and kidneys of chickens and cause disease and death,and H120 immunization could not provide effective protection against the two IBV isolates.It is noteworthy that the pathogenicity of SX/2204 has significantly increased compared to the GVI-1 strains isolated previously,with a mortality rate up to 60%.Considering the continuous mutation and recombination of the IBV genome to produce new variant strains,it is important to continuously monitor epidemic strains and develop new vaccines for the prevention and control of IBV epidemics.

关键词： infectious bronchitis virus GI-19 lineage GVI-1 lineage complete genome recombination pathogenicity

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Genetic and biological properties of H9N2 avian influenza viruses isolated in central China from 2020 to 2022

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Journal of Integrative Agriculture 2024年第8期 2778-2791页

作者： Libin Liang Yaning Bai Wenyan huang Pengfei Ren Xing Li Dou Wang Yuhan Yang Zhen Gao Jiao Tang Xingchen Wu Shimin Gao Yanna Guo mingming hu Zhiwei Wang Zhongbing Wang Haili Ma Junping Li College of Veterinary Medicine Shanxi Agricultural University Shanxi Provincial Animal Disease Prevention and Control Center

TheH9N2subtypeofavianinfluenzavirus(AIV)iswidelyprevalentinpoultryandwildbirdsglobally,andhas become the predominant subtype circulating in poultry in China.The H9N2 AIV can directly or indirectly (by serving asa“donorvirus”)infecthumans,posingasignificantthreattopublichealth.Currently,thereisalackofindepth research on the prevalence of H9N2 viruses in Shanxi Province, central China.In this study, we isolated 14H9N2 AIVs from October 2020 to April 2022 in Shanxi Province, and genetic analysis revealed that these viruses belonged to 7 different genotypes.Our study on animals revealed that the H9N2 strains we identified displayed hightransmissionefficiencyamongchickenpopulations,andexhibiteddiversereplicationabilitieswithinthese birds.These viruses could replicate efficiently in the lungs of mice, with one strain also demonstrating the capacity to reproduce in organs like the brain and kidneys.At the cellular level, the replication ability of different H9N2 strains was evaluated using plaque formation assays and multi-step growth curve assays, revealing significant differences inthereplicationandproliferationefficiencyofthevariousH9N2virusesatthecellularlevel.Theantigenicity analysis suggested that these isolates could be classified into 2 separate antigenic clusters.Our research provides crucial data to help understand the prevalence and biological characteristics of H9N2 AIVs in central China.It also highlights the necessity of enhancing the surveillance of H9N2 AIVs.

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The SACT Template:A human Brain Diffusion Tensor Template for School-age Children

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Neuroscience Bulletin 2022年第6期38卷 607-621页

作者： Congying Chu Haoran Guan Sangma Xie Yanpei Wang Jie Luo Gai Zhao Zhiying Pan mingming hu Weiwei Men Shuping Tan Jia-Hong Gao Shaozheng Qin Yong He Lingzhong Fan Qi Dong Sha Tao National Key Laboratory of Cognitive Neuroscience and Learning&IDG/McGovern Institute for Brain Research Beijing Normal UniversityBeijing 100875China Bejing Key Laboratory of Brain Imaging and Connectomics Beijing Normal UniversityBejing 100875China Center for MRI Research Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijing 100871China Beijing City Key Laboratory for Medical Physics and Engineering Institute of Heavy lon PhysicsSchool of PhysicsPeking UniversityBeijing 100871China MeGovern Institute for Brain Research Peking UniversityBeijing 100871China Psychiatry Research Center Bejing HuiLongGuan HospitalPeking UniversityBejing 100096China Division of Molecular Neuroimaging Institute of Neuroscience and Medicine-2Research Center Julich52425 JilichGermany Institute of Biomedical Engineering and Instrumentation School of AutomationHangzhou Dianzi UniversityHangzhou 310018China Brainnetome Center and National Laboratory of Pattern Recognition Institute of AutomationChinese Academy of SciencesBeijing 100190China

School-age children are in a specific development stage corresponding to juvenility,when the white matter of the brain experiences ongoing maturation.Dffusion-weighted magnetic resonance imaging(DWI),especially diffusion tensor imaging(DTI),is extensively used to characterize the maturation by assessing white matter properties in vivo.In the analysis of DWI data,spatial normalization is crucial for conducting inter-subject analyses or linking the individual space with the reference space.Using tensor-based registration with an appropriate diffusion tensor template presents high accuracy regarding spatial normalization.However,there is a lack of a standardized diffusion tensor template dedicated to school-age children with ongoing brain development.Here,we established the school-age children diffusion tensor(SACT)template by optimizing tensor reorientation on high-quality DTI data from a large sample of cognitively normal participants aged 6-12 years.With an age-balanced design,the SACT template represented the entire age range well by showing high similarity to the age-specific templates.Compared with the tensor template of adults,the SACT template revealed significantly higher spatial normalization accuracy and inter-subject coherence upon evaluation of subjects in two different datasets of schoolage children.A practical application regarding the age associations with the normalized DTI-derived data was conducted to further compare the SACT template and the adult template.Although similar spatial patterns were found,the SACT template showed significant effects on the distributions of the statistical results,which may be related to the performance of spatial normalization.Looking forward,the SACT template could contribute to future studies of white matter development in both healthy and clinical populations.The SACT template is publicly available now(tp://igshare com/aricles/dataseu'SACT_.template/14071283).

关键词： School-age children Diffusion-weighted MRI Diffusion tensor template Spatial normalization

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Preparation, characterization, and in vitro/vivo evaluation of polymer-assisting formulation of atorvastatin calcium based on solid dispersion technique

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Asian Journal of Pharmaceutical Sciences 2018年第6期13卷 546-554页

作者： Wenxiang Dong Xitong Su Meng Xu mingming hu Yinghua Sun Peng Zhang Shenyang Pharmaceutical University

Due to low solubility and bioavailability, atorvastatin calcium is confronted with challenge in conceiving appropriate formulation. Solid dispersion of atorvastatin calcium was prepared through the solvent evaporation method, with Poloxamer 188 as hydrophilic carriers. This formulation was then characterized by scanning electron microscopy, differential scanning calorimetry, powder X-ray diffraction and fourier transform infrared spectroscopy. Moreover, all these studies suggested the conversion of crystalline atorvastatin calcium. In addition, the drug solubility studies as well as dissolution rates compared with bulk drug and market tablets Lipitor were also examined. Furthermore, the study investigated the pharmacokinetics after oral administration of Lipitor and solid dispersion. And the AUC 0–8 h and C max increased after taking ATC-P188 solid dispersion orally compared with that of Lipitor. All these could be demonstrated that ATC-P188 solid dispersions would be prospective means for enhancing higher oral bioavailability of ATC.

关键词： Atorvastatin calcium Poloxamer 188 Solid dispersion Dissolution rate Oral bioavailability

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Evaluation of micelles incorporated into thermosensitive hydrogels for intratumoral delivery and controlled release of docetaxel:A dual approach for in situ treatment of tumors

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Asian Journal of Pharmaceutical Sciences 2018年第4期13卷 373-382页

作者： Meng Xu Yanhua Mou mingming hu Wenxiang Dong Xitong Su Rongxiaa Wu Peng Zhang School of Pharmacy Shenyang Pharmaceutical University Department of Pharmacology Shenyang Pharmaceutical University

The in situ gelling hybrid hydrogel system has been reported to effectively concentratechemotherapeutic drugs at the tumor site and sustain their release for a long period. DTX-micelles(docetaxel-loaded mixed micelles) are able to increase the solubility of DTX inwater, and then a high drug loading rate of hydrogels can be achieved by encapsulatingthe docetaxel-loaded mixed micelles into the hydrogels. The thermosensitive nature ofDTX-MM-hydrogels(thermosensitive hydrogels incorporated with docetaxel-loaded mixedmicelles) can accelerate the formation of a depot of this drug-loaded system at the siteof administration. Therefore, the hydrogels provide a much slower release compared withDTX-micelles and DTX-injection. An in vivo retention study has demonstrated that the DTX-MM-hydrogels can prolong the drug retention time and in viv o trials have shown that theDTX-MM-hydrogels have a higher antitumor efficacy and systemic safety. In conclusion, theDTX-MM-hydrogels prepared in this study have considerable potential as a drug deliverysystem, with higher tumor inhibition effects and are less toxic to normal tissues.

关键词： Docetaxel Thermosensitive Micelles Hydrogels Intratumoral

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低围压双向压缩条件下竖直裂纹扩展机制研究

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矿业研究与开发 2024年第1期44卷 104-111页

作者：王家豪汪学清高阳胡明明中国矿业大学(北京)力学与建筑工程学院北京100083 中国科学院武汉岩土力学研究所湖北武汉430071

为研究地下洞室开挖等岩石工程中双向压缩应力状态下的岩体裂纹扩展机制,以低围压三向受压或单轴压缩条件下含竖直裂纹的岩体试样为研究对象,通过数值模拟的方式分析不同泊松比μ和裂纹迹长a条件下远场轴向压应力和裂纹尖端应力的分布... 详细信息

为研究地下洞室开挖等岩石工程中双向压缩应力状态下的岩体裂纹扩展机制,以低围压三向受压或单轴压缩条件下含竖直裂纹的岩体试样为研究对象,通过数值模拟的方式分析不同泊松比μ和裂纹迹长a条件下远场轴向压应力和裂纹尖端应力的分布规律。基于叠加原理得到双向压缩条件下竖直裂纹/翼裂纹尖端近场应力的表达式,并推导出该应力状态下裂纹岩体试样的应力强度因子及裂纹扩展方向。结果表明:存在远场轴向压应力时,泊松比μ和裂纹迹长a对裂纹尖端应力分布规律有明显影响。裂纹尖端应力σxx与平行于裂纹面的轴向压应力σn的比值与泊松比μ成一次函数关系,与裂纹迹长a成三次函数关系。

关键词：双向压缩应力远场轴向压应力近场应力泊松比裂纹迹长

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基于电子鼻和溶剂辅助风味蒸发-气相色谱-质谱联用技术分析调味小龙虾挥发性风味特征差异

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食品与发酵工业 2024年第8期50卷 242-252页

作者：张权李金林胡明明彭斌钟比真涂宗财江西师范大学生命科学院江西南昌330022 江西师范大学国家淡水鱼加工技术研发专业中心江西南昌330022 南昌大学食品科学与资源挖掘全国重点实验室江西南昌330047

为探究调味小龙虾风味特征差异,该文以市售常见调味小龙虾为研究对象,采用电子鼻结合溶剂辅助风味蒸发-气相色谱-质谱联用(solvent-assisted flavor evaporation-gas chromatography-mass spectrometry,SAFE-GC-MS)技术对其挥发性风味... 详细信息

为探究调味小龙虾风味特征差异,该文以市售常见调味小龙虾为研究对象,采用电子鼻结合溶剂辅助风味蒸发-气相色谱-质谱联用(solvent-assisted flavor evaporation-gas chromatography-mass spectrometry,SAFE-GC-MS)技术对其挥发性风味成分进行分析。结果表明,电子鼻能够较好地区分不同调味小龙虾,W1W(对无机硫化物灵敏)、W1S(对甲基类灵敏)和W2W(对芳香成分、有机硫化物灵敏)是区分调味小龙虾风味的主要传感器。8个调味小龙虾样品中共检出127种挥发性风味物质,通过相对气味活度值(relative odor activity value,ROAV)法确定了15种关键风味物质和10种修饰性风味物质,其中麻辣小龙虾主要关键风味物质为芳樟醇、桉叶油醇、丁香酚和D-柠檬烯,十三香小龙虾主要关键风味物质主要为丁香酚、芳樟醇、桉叶油醇、壬醛和β-蒎烯,而蒜蓉小龙虾主要关键风味物质为2,4-二叔丁基苯酚、L-石竹烯、壬醛、苯乙醛、2-甲基-2-丁烯醛和二烯丙基硫醚等。该研究结果可为调味小龙虾风味统一、产品开发和生产提供理论参考。

关键词：小龙虾电子鼻溶剂辅助风味蒸发-气相色谱-质谱联用挥发性风味物质相对气味活度值

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The studies of PLGA nanoparticles loading atorvastatin calcium for oral administration in vitro and in vivo

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Asian Journal of Pharmaceutical Sciences 2017年第3期12卷 285-291页

作者： Zhenbao Li Wenhui Tao Dong Zhang Chunnuan Wu Binbin Song Shang Wang Tianyang Wang mingming hu Xiaohong Liu Yongjun Wang Yinghua Sun Jin Sun School of Pharmacy Shenyang Pharmaceutical UniversityNo.103Wenhua RoadShenyang 110016China Tianjin Medical University Cancer Hospital Tianjin 300060China China Resources Double-crane Pharmaceutical Co. Ltd.No.1Wangjing Lize East RoadChaoyang DistrictBeijingChina. Municipal Key Laboratory of Biopharmaceutics School of PharmacyShenyang Pharmaceutical UniversityNo.103Wenhua RoadShenyang 110016China

A biodegradable poly(lactic-co-glycolic acid) loading atorvastatin calcium(AC) nanoparticles(AC-PLGA-NPs) were prepared by probe ultrasonication and evaporation method aiming at improving the oral bioavailability of AC. The effects of experimental parameters, including stabilizer species, stabilizer concentration and pH of aqueous phase, on particle size were also evaluated. The resultant nanoparticles were in spherical shape with an average diameter of 174.7 nm and a narrow particle size distribution. And the drug loading and encapsulation efficiency were about 8% and 71%, respectively. The particle size and polydispersion were almost unchanged in 10 days. The release curves of AC-PLGA-NPs in vitro displaying sustained release characteristics indicated that its release mechanisms were matrix erosion and diffusion. The pharmacokinetic study in vivo revealed that the Cmax and AUC0-∞ of AC-PLGA-NPs in rats were nearly 3.7-fold and 4.7-fold higher than that of pure atorvastatin calcium suspension. Our results demonstrated that the delivery of AC-PLGANPs could be a promising approach for the oral delivery of AC for enhanced bioavailability.

关键词： Atorvastatin calcium AC-PLGA-NPs Probe ultrasonication and evaporation method Oral bioavailability

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对凹折式挡土墙设计推力的探讨

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矿业研究与开发 2024年第2期44卷 157-163页

作者：王家豪高阳汪学清胡明明赵阳中国矿业大学(北京)力学与建筑工程学院北京100083 中国科学院武汉岩土力学研究所湖北武汉430071 华北水利水电大学资源与环境学院河南郑州450011

挡土墙是整治矿山渣坡的主要措施之一,重力式挡土墙凭借其就地取材、施工方便、经济效果好的优点得到了广泛应用。重力式挡土墙样式繁多,但对于某些高度大于20 m的挡土墙,常规形式无法满足承载力和稳定性要求,实际工程中往往采用凹折式... 详细信息

挡土墙是整治矿山渣坡的主要措施之一,重力式挡土墙凭借其就地取材、施工方便、经济效果好的优点得到了广泛应用。重力式挡土墙样式繁多,但对于某些高度大于20 m的挡土墙,常规形式无法满足承载力和稳定性要求,实际工程中往往采用凹折式挡土墙。通过分析凹折式挡土墙的形状类型,对凹折式挡土墙墙后土体进行分块,根据块体的应力分析,推导得出凹折式挡土墙滑坡推力和主动土压力的计算公式,并将其应用于某锰渣填埋场2#渣坝。结果表明,天然工况、暴雨工况和地震工况下凹折式挡土墙的主动土压力数值远大于滑坡推力数值,3种工况下计算公式得到的抗滑移稳定安全系数均大于规范要求的抗滑移稳定安全系数,表明计算公式能很好地应用于凹折式挡土墙,也可以推广应用于墙背为弧形的特殊挡土墙。

关键词：凹折式挡土墙滑坡推力主动土压力抗滑移稳定安全系数

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路堤结合海塘的结构设计要点

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浙江水利科技 2024年第2期52卷 20-24页

作者：梁佳春孟海南胡明明杭州市西湖区钱塘江江堤管理服务中心浙江杭州310024

当前,新一轮海塘建设中把建设安全可靠、绿色生态、功能综合、运行高效的海塘工程作为目标,不少海塘结合提标融合了诸如交通、绿道等功能。由于海塘功能的融合,给设计提出不少要求。以西湖区南北塘路堤结合海塘为例,从两者结构融合与设... 详细信息

当前,新一轮海塘建设中把建设安全可靠、绿色生态、功能综合、运行高效的海塘工程作为目标,不少海塘结合提标融合了诸如交通、绿道等功能。由于海塘功能的融合,给设计提出不少要求。以西湖区南北塘路堤结合海塘为例,从两者结构融合与设计标准兼顾入手,简要阐述路堤结合海塘断面主要设计要点。

关键词：路堤结合结构设计功能融合

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