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Antifungal activity of diacetyl, a volatile organic compound, on Trichoderma lixii F2 isolated from postharvest Lanzhou lily bulbs

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Food Bioscience 2023年第2期52卷 193-202页

作者： Lijun Ling mingmei pang Hong Luo Wenting Cheng KunLing Jiang Yuanyuan Wang College of Life Sciences Northwest Normal UniversityLanzhou730070PR China The Rural Development Academy Northwest Normal UniversityPR China

The most severe fungal disease is postharvest gray mold,which might cause massive losses in Lanzhou lily *** microorganism-produced volatile organic compounds(VOCs)have been proven to have antifungal action and can be employed as fruit and vegetable *** most recent research discovered that diacetyl(2,3-butanedione)produced by Bacillus subtilis CL2 could significantly prevent some *** goal of this investigation was to see if diacetyl had antifungal properties against Trichoderma lixii F2,one of the most common postharvest rot pathogens of Lanzhou lily *** results indicated that diacetyl(1.587μL/mL)fumigation treatment significantly inhibited the hyphal growth,modified hyphal morphology,and disrupted the cell membrane *** vivo tests the antifungal mechanisms of diacetyl on *** F2 were performed in Lanzhou lily *** results indicated that the content of total phenols and flavonoids increased,while malondialdehyde decreased under different treatment by ***,the activities of peroxidase,polyphenol oxidase,catalase,and superoxide dismutase and phenylalanine ammonia-lyase in Lanzhou lily bulbs were *** was showed that diacetyl could eliminate excessive reactive oxygen species,reduce the damage of plant cells,and improve the resistance of Lanzhou lily bulbs against *** *** research reveals that diacetyl could activate antioxidant enzymes and disease-resistant enzymes in Lanzhou lily bulbs to prevent pathogenic fungus from invading and inducing resistance,laying the groundwork for future uses in preserving Lanzhou lily bulbs.

关键词： Diacetyl Lanzhou lily bulbs Postharvest preservation Volatile organic compounds Inhibitory effects

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在果模艺术中应用木版画造型元素的时代价值

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文化创新比较研究 2020年第27期4卷 91-93页

作者：王怡薛巍逄铭梅青岛黄海学院

近年来,随着时代的发展,"速食文化"深入人心,以山东果模艺术为代表的民间非物质文化遗产受其影响,对文化的保护与传承产生了新的时代需求。在果模艺术中应用木版画造型元素,可以简化果模造型元素,满足"短时间,高趣味"的优势条件,有利于... 详细信息

近年来,随着时代的发展,"速食文化"深入人心,以山东果模艺术为代表的民间非物质文化遗产受其影响,对文化的保护与传承产生了新的时代需求。在果模艺术中应用木版画造型元素,可以简化果模造型元素,满足"短时间,高趣味"的优势条件,有利于在社会中推广、普及果模艺术,对果模艺术的保护与传承有着重要的时代价值。

关键词：山东果模非物质文化遗产木版画造型元素时代价值

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黄花蒿精油及其单体桉叶油醇对兰州百合采后病害的防治效果

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西北农林科技大学学报（自然科学版） 2022年第8期50卷 77-87页

作者：令利军程文婷蒋坤玲王圆圆罗鸿杨彩云庞明梅马稳霞西北师范大学生命科学学院甘肃兰州730070

【目的】利用黄花蒿精油及其单体桉叶油醇(1,8-cineole)对兰州百合采后病害进行防治,为延长兰州百合贮存期提供参考。【方法】以新鲜兰州百合、黄花蒿精油及其单体桉叶油醇为材料,以病原细菌摩加夫芽孢杆菌(B-6)和病原真菌李氏木霉(F-2... 详细信息

【目的】利用黄花蒿精油及其单体桉叶油醇(1,8-cineole)对兰州百合采后病害进行防治,为延长兰州百合贮存期提供参考。【方法】以新鲜兰州百合、黄花蒿精油及其单体桉叶油醇为材料,以病原细菌摩加夫芽孢杆菌(B-6)和病原真菌李氏木霉(F-2)、篮状菌(F-3)和镰刀菌(F-6)为靶标菌,采用倍比稀释法测定黄花蒿精油及其单体桉叶油醇对兰州百合病原细菌B-6的最小抑菌浓度和最小杀菌浓度;采用熏蒸法测定黄花蒿精油及其单体桉叶油醇对3种病原真菌的抑制率,并建立毒力回归方程;采用扫描电镜观察黄花蒿精油及其单体桉叶油醇对病原菌细胞壁的影响;分别用黄花蒿精油及其单体桉叶油醇处理含有病原细菌悬浮液和病原真菌孢子悬浮液的兰州百合,筛选最佳的防治浓度;测定兰州百合自由基清除率,褐变度,总酚、类黄酮、可溶性总糖和丙二醛含量及保护酶活性。【结果】抑菌结果显示,黄花蒿精油及其单体桉叶油醇对兰州百合病原细菌B-6具有较强的抑制作用,其对病原细菌B-6的MIC值均为1.25 mL/L,MBC值分别为1.25和5.00 mL/L;黄花蒿精油及其单体桉叶油醇对兰州百合病原真菌均有较强的毒力作用,黄花蒿精油和桉叶油醇对F-2、F-3、F-6的EC_(50)分别为0.314,0.336,0.357 mL/L和0.280,0.289,0.420 mL/L。扫描电镜结果显示,对照组病原细菌细胞菌体完整,而黄花蒿精油及其单体桉叶油醇处理细菌单个菌体不完整,大量菌体堆积,内容物渗出;对照组病原真菌菌丝为均匀而规则的管状,而黄花蒿精油及其单体桉叶油醇处理菌丝表面出现大量褶皱、断裂及扭曲。黄花蒿精油和桉叶油醇对接种病原菌后的兰州百合最佳防治浓度均为15.38μL/L。生理生化指标测定结果显示,黄花蒿精油及其单体桉叶油醇均能抑制兰州百合自身抗氧化活性的下降,降低兰州百合的褐变率,减少MDA积累,增加SOD活性,抑制POD和PPO活性。【结论】黄花蒿精油及其单体桉叶油醇具有良好的抑菌能力和抗氧化活性,对兰州百合的最佳防治浓度均为15.38μL/L。

关键词：黄花蒿精油桉叶油醇(1,8-cineole) 兰州百合病害防治采后病害百合保鲜

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Dissymmetric flow phenomenon in a multistrand tundish

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Journal of University of Science and Technology Beijing 2007年第6期14卷 490-494页

作者： mingmei Zhu Guanghua Wen Ping Tang Yufeng Wang College of Material Science and Engineering Chongqing University Chongqing 400044 China

The dissymmetric flow phenomenon exists in a symmetric multistrand tundish. It was studied by the physical simulation experiment. The fundamental flow characteristic of dissymmetry was analyzed. The asymmetry of the flow field, the temperature field, and the inclusions distribution without flow-control devices （FCDs） were compared with those with FCDs. It is proved that the asymmetry of the flow and temperature field along the outlets at the long range is more obvious. The symmetric FCDs installation has a slight effect on the dissymmetric temperature field, simultaneously, the symmetry of the average residence time and the fluid flow pattern has improved, and the fluid flow in the tundish has been more reasonable. In case of a symmetric multistrand tundish having a large volume, the influence of the dissymmetric phenomenon should be considered and the flow behaviors in the whole tundish should be studied completely.

关键词： multistrand tundish dissymmetry flow phenomenon physical simulation flow-control devices

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Improving the accuracy of mechanistic models for dynamic batch distillation enabled by neural network:An industrial plant case

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Chinese Journal of Chemical Engineering 2024年第9期73卷 290-300页

作者： Xiaoyu Zhou Xiangyi Gao mingmei Wang Erwei Song Erqiang Wang School of Chemical Engineering University of Chinese Academy of SciencesBeijing 100049China School of Information Science and Engineering Yanshan UniversityQinhuangdao 066004China

Neural networks are often viewed as pure‘black box’models,lacking interpretability and extrapolation capabilities of pure mechanistic *** work proposes a new approach that,with the help of neural networks,improves the conformity of the first-principal model to the actual *** final result is still a first-principal model rather than a hybrid model,which maintains the advantage of the high interpretability of first-principal *** work better simulates industrial batch distillation which separates four components:water,ethylene glycol,diethylene glycol,and triethylene ***(gated recurrent neural network)and LSTM(long short-term memory)were used to obtain empirical parameters of mechanistic model that are difficult to measure *** were used to improve the empirical processes in mechanistic model,thus correcting unreasonable model assumptions and achieving better predictability for batch *** proposed method was verified using a case study from one industrial plant case,and the results show its advancement in improving model predictions and the potential to extend to other similar systems.

关键词： Batch distillation Mechanistic models Neural network GRU LSTM

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Quality control for ocean observations: From present to future

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Science China Earth Sciences 2022年第2期65卷 215-233页

作者： Zhetao TAN Bin ZHANG Xiaofen WU mingmei DONG Lijing CHENG International Center for Climate and Environment Sciences Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China Institute of Oceanology Chinese Academy of SciencesQingdao 266071China State Key Laboratory of Satellite Ocean Environment Dynamics Second Institute of OceanographyMinistry of Natural ResourcesHangzhou 310012China National Marine Data and Information Service Tianjin 300171China University of Chinese Academy of Sciences Beijing 100049China

The quality control(QC) of ocean observational data, essential to establish a high-quality global ocean database, is one of the basic data pre-processing steps in oceanography research, marine monitoring, and forecasting. With the introduction of various advanced instruments in recent decades, oceanographic surveys have expanded from coastal regions to open ***, as ocean in-situ observations are obtained using different instruments that offer heterogeneous data qualities, it is paramount that bad data could be accurately and efficiently identified via QC to provide a reliable global ocean database. In this review, we briefly summarize the latest progress of QC for oceanic in-situ observations, and mainly focus on temperature and salinity data. The similarities and differences between QC schemes developed by various ocean organizations are introduced. We also discuss the performances of the various QC schemes and identify the key challenges. Based on the discussions, several recommendations are proposed for future improvements in the QC for ocean observations.

关键词： Quality control Ocean observations Temperature Salinity Data quality Big data

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Efficient removal for multiple pollutants via Ag_(2)O/BiOBr heterojunction:A promoted photocatalytic process by valid electron transfer pathway

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Chinese Chemical Letters 2020年第10期31卷 2698-2704页

作者： Zhiruo Zhou Yanan Li mingmei Li Yi Li Sihui Zhan College of Environmental Science and Engineering Key Laboratory of Pollution Processes and Environmental CriteriaTianjin Key Laboratory of Environmental Remediation and Pollution ControlNankai UniversityTianjin 300071China Tianjin Key Laboratory of Molecular Optoelectronic Sciences Department of ChemistrySchool of ScienceTianjin University&Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)Tianjin 300072China Tianjin Key Lab for Rare Earth Materials and Applications Tianjin 300072China

Multiple pollutants including pathogenic microorganism contaminations and emerging organic contaminations(EOCs)have shown a growing threat to the environment,especially the natural ***,the control and removal of pathogenic microorganism contaminations and EOCs have been greatly limited since limited knowledge of their environmental ***,a novel and efficient photocatalyst Ag_(2)O/BiOBr heterojunction was synthesized and used for removal of multiple pollutants including Escherichia coli(***),Staphylococcus aureus(***),tetracycline and acetaminophen under visible *** results showed that there were valid electron transfer pathways between BiOBr and Ag_(2)O,the main electron transfer direction was the BiOBr to Ag_(2)***-generated electrons were stored in Ag_(2)O and thus separation efficiency between holes and photo-generated electrons was obviously *** oxygen species were highly produced and eventually end up with the high efficiency of removal of multiple *** Ag_(2)O/BiOBr with Ag_(2)O content at 3%(the best performance)under visible light,log decrease of *** was 7.16(removal efficiency was 100%)in 120 min,log decrease of *** was 7.23(removal efficiency was 100%)in 160 min,C/C0 of tetracycline was 0.06 in 180 min,C/C0 of acetaminophen was 0.17 in 180 *** work could provide a promising candidate in the actual contaminated natural waters for cleaning multiple pollutants.

关键词： Water pollution Photocatalysis Multiple pollutants Electrons transfer channels

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Surface display of carbonic anhydrase on Escherichia coli for CO_(2) capture and mineralization

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Synthetic and Systems Biotechnology 2022年第1期7卷 460-473页

作者： Yinzhuang Zhu Yaru Liu mingmei Ai Xiaoqiang Jia Department of Biochemical Engineering School of Chemical Engineering and TechnologyTianjin UniversityTianjin300072PR China Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(MOE) School of Chemical Engineering and TechnologyTianjin UniversityTianjin300350PR China Collaborative Innovation Center of Chemical Science and Engineering(Tianjin) Tianjin300072PR China

Mineralization catalyzed by carbonic anhydrase(CA)is one of the most promising technologies for capturing CO_(2).In this work,Escherichia coli BL21(DE3)was used as the host,and the N-terminus of ice nucleation protein(INPN)was used as the carrier *** fusion patterns and vectors were used to construct CA surface display systems forα-carbonic anhydrase(HPCA)from Helicobacter pylori 26695 andα-carbonic anhydrase(SazCA)from Sulfurihydrogenibium *** surface display system in which HPCA was fused with INPN via a flexible linker and intermediate repeat sequences showed higher whole-cell enzyme activity,while the enzyme activity of the SazCA expression system was significantly higher than that of the HPCA expression *** pET22b vector with the signal peptide PelB was more suitable for the cell surface display of *** frac-tionation and western-blot analysis indicated that SazCA and INPN were successfully anchored on the cell’s outer membrane as a fusion *** enzyme activity of the surface display strain E-22b-I RL S(11.43 U⋅mL^(−1) OD 600−1)was significantly higher than that of the intracellular expression strain E-22b-S(8.355 U⋅mL^(−1) OD 600−1)under optimized induction *** with free SazCA,E-22b-I RL S had higher thermal and pH *** long-term stability of SazCA was also significantly improved by surface *** the engineered strain and free enzyme were used for CO_(2) mineralization,the amount of CaCO_(3) deposition catalyzed by the strain E-22b-I RL S on the surface(241 mg)was similar to that of the free SazCA and was significantly higher than the intracellular expression strain E-22b-S(173 mg).These results demonstrate that the SazCA surface display strain can serve as a whole-cell biocatalyst for CO_(2) capture and mineralization.

关键词： Carbonic anhydrase Cell surface display Enzyme stability CO_(2)mineralization Ice nucleation protein

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Mathematical modeling of fluid flow, heat transfer and inclusion transport in a four strand tundish

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Journal of University of Science and Technology Beijing 2007年第1期14卷 22-26页

作者： Yufeng Wang Guanghua Wen Ping Tang mingmei Zhu Yuanqing Chen Weizhao Song College of Material Science and Engineering Chongqing University Chongqing 400044China Technical Center Xinjiang Bayi Iron and Steel Co.LtdUrumchi 830022China

Mathematical simulation was used for trouble-shooting and optimization. By the mathematical simulation, fluid flow and beat transfer of molten-steel in a four-strand tundish of a billet caster under different conditions （bare tundish and tundish with flow control device） were analyzed, The results showed that （1） the tundish with flow control device （FCD） has an important effect on the fluid pattern and temperature distribution; （2） the unsteady, solving method was used to model the inclusion motions at different time perthds, and it showed that the FCD is advantageous to separate the nonmetallic inclusions. According to the simulation results, the main problem existing in the industry preduction was found, and some helpful rneasurements were executed. Consequently, the large nonmetallic inclusions were separated, and the content of total oxygen was reduced. The qualily of steel was greatly improved.

关键词： mathematical simulation four strand tundish fluid flow temperature distribution inclusion transpori

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An Fe-N/S-C hybrid electrocatalyst derived from bimetal-organic framework for efficiently electrocatalyzing oxygen reduction reaction in acidic media

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Journal of Energy Chemistry 2021年第1期30卷 291-300,I0010页

作者： Shuqin Song mingmei Wu Junwei Chen Zhuohua Mo Rui Chen Kun Wang Tongwen Yu The Key Lab of Low-carbon Chemistry&Energy Conservation of Guangdong Province School of Materials Science and EngineeringSchool of ChemistrySchool of Chemical Engineering and TechnologySun Yat-sen UniversityGuangzhou 510275GuangdongChina

Heteroatoms doped Fe-N-C electrocatalysts have been widely acknowledged as one of the most promising candidates to replace Pt-based materials for electrocatalyzing oxygen reduction reaction(ORR).However,the complicated synthesis method and controversial catalytic mechanism represent a substantial impediment as of ***,a very facile strategy to prepare Fe-N/S-C hybrid through pyrolyzing Zn and Fe bimetallic MOFs is rationally *** electrocatalytic ORR performance shows a volcanotype curve with the increment of added Fe *** half-wave potential(E1/2) for ORR at optimized Fe-N/S-C-10%(10%=n(Fe)/(n(Fe)+n(Zn)),n(Fe) and n(Zn) represent the moles of Fe2+ and Zn2+ in the precursors,respectively) shifts significantly to the positive direction of 19.6 mV with respect to that of Pt/C in acidic media,as well as a high 4 e selectivity and methanol *** 10,000 potential cycles,E1/2 exhibits a small negative shift of-27.5 mV at Fe-N/S-C-10% compared favorably with Pt/C(~141.0 mV).This can be attributed to:(ⅰ) large specific surface area(849 m^(2)/g) and hierarchically porous structure are favorable for the rapid mass transfer and active sites exposure;(ⅱ) the embedded Fecontaining nanoparticles in porous carbon are difficult to be moved and further agglomerated during the electrochemical accelerated aging test,further improving its stability;(ⅲ) there exist small Fecontaining nanoparticles,uniformly doped N and S,abundant Fe-N as efficiently active *** work represents a breakthrough in the development of high-efficient non-precious-metal catalysts(NPMCs)to address the current Pt-based electrocatalysts challenges.

关键词： Bimetallic MOFs Fe-N/S-C Oxygen reduction reaction Acidic media

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