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Genome Analysis of Dasineura jujubifolia Toursvirus 2,A Novel Ascovirus

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Virologica Sinica 2020年第2期35卷 134-142页

作者： Jun Wang minglu yang Haibing Xiao Guo-Hua Huang Fei Deng Zhihong Hu State Key Laboratory of Virology and National Virus Resource Center Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China Tarim University Alar 843300China Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests Institute of VirologyHunan Agricultural UniversityChangsha 410128China

So far,ascoviruses have only been identified from Lepidoptera host insects and their transmission vectors-endoparasitic wasps.Here,we reported the first finding of a complete novel ascovirus genome from a Diptera insect,Dasineura jujubifolia.Initially,sequence fragments with homology to ascoviruses were incidentally identified during metagenomic sequencing of the mitochondria of D.jujubifolia(Cecidomyiidae,Diptera)which is a major pest on Ziziphus jujuba.Then a full circular viral genome was assembled from the metagenomic data,which has an A+T percentage of 74%and contains 142,600 bp with 141 open reading frames(ORFs).Among the 141 ORFs,37 were conserved in all sequenced ascoviruses(core genes)including proteins predicted to participate in DNA replication,gene transcription,protein modification,virus assembly,lipid metabolism and apoptosis.Multi-gene families including those encode for baculovirus repeated open reading frames(BROs),myristylated membrane proteins,RING/U-box E3 ubiquitin ligases,and ATP-binding cassette(ABC)transporters were found in the virus genome.Phylogenetic analysis showed that the newly identified virus belongs to genus Toursvirus of Ascoviridae,and is therefore named as Dasineura jujubifolia toursvirus 2(DjTV-2a).The virus becomes the second reported species of the genus after Diadromus pulchellus toursvirus 1(DpTV-1a).The genome arrangement of DjTV-2a is quite different from that of DpTV-1a,suggesting these two viruses separated in an early time of evolution.The results suggest that the ascoviruses may infect a much broader range of hosts than our previous knowledge,and shed lights on the evolution of ascoviruses and particularly on that of the toursviruses.

关键词： Dasineura jujubifolia toursvirus 2(DjTV-2a) Ascovirus Toursvirus

新零售行业创业企业个案研究——以兴盛优选为例

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科技创新与生产力 2024年第1期45卷 46-48,52页

作者：杨铭璐刘王滢郭云贵湖南科技大学商学院湖南湘潭411201

本文通过对兴盛优选的创业个案进行研究,总结出兴盛优选创业的成功因素和后续发展过程中所面临的问题,并以此归纳出对创业者的启示:在创业过程中要找准目标用户,增强核心竞争力,并提高产品质量。

关键词：新零售社区团购兴盛优选

阿拉尔棉田烟粉虱捕食性天敌的SCAR分子标记检测

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现代农业科技 2024年第13期 161-164,172页

作者：赵宇杨清川梁静孙雨杨晓东杨明禄塔里木大学农学院新疆阿拉尔843300 南疆农业有害生物综合治理兵团重点实验室新疆阿拉尔843300

为了明确阿拉尔垦区棉田烟粉虱种群隐种以及烟粉虱捕食性天敌种类,采集阿拉尔十二团棉田中的烟粉虱及烟粉虱捕食性天敌,利用B-F/B-R、Q-F/Q-R特异性引物检测烟粉虱隐种类型,利用烟粉虱TB-F94585/TB-R 94585特异性引物检测捕食性天敌肠... 详细信息

为了明确阿拉尔垦区棉田烟粉虱种群隐种以及烟粉虱捕食性天敌种类,采集阿拉尔十二团棉田中的烟粉虱及烟粉虱捕食性天敌,利用B-F/B-R、Q-F/Q-R特异性引物检测烟粉虱隐种类型,利用烟粉虱TB-F94585/TB-R 94585特异性引物检测捕食性天敌肠道内是否存在烟粉虱DNA。结果表明:新疆阿拉尔垦区烟粉虱为MED隐种,初步发现烟粉虱的捕食天敌共有4目5科,包括多异瓢虫成虫、多异瓢虫幼虫、东亚小花蝽、中华草蛉幼虫、黄褐新园蛛、灌木新园蛛、直伸肖蛸。利用SCAR分子标记技术可以快速检测阿拉尔垦区烟粉虱捕食性天敌种类。

关键词：烟粉虱 SCAR分子标记捕食性天敌棉田新疆阿拉尔

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槐豆木虱形态特征及生物学特性的研究

中国农学通报 2024年第14期40卷 112-118页

作者：梁静伍兴隆杨晓东孙雨赵宇杨清川任洁肖海兵杨明禄塔里木大学农学院新疆阿拉尔843300 南疆农业有害生物综合治理兵团重点实验室新疆阿拉尔843300

本研究旨在探讨槐豆木虱的形态特征及其生物学特性,为该害虫的综合防治提供科学依据。通过若虫蜕皮情况确定其龄期,观察并记录卵至成虫的发育历期、繁殖力及生活习性,利用体视显微镜拍照并测量各发育阶段的形态变化。结果显示,槐豆木虱... 详细信息

本研究旨在探讨槐豆木虱的形态特征及其生物学特性,为该害虫的综合防治提供科学依据。通过若虫蜕皮情况确定其龄期,观察并记录卵至成虫的发育历期、繁殖力及生活习性,利用体视显微镜拍照并测量各发育阶段的形态变化。结果显示,槐豆木虱卵呈纺锤形,长(362.13±3.53)μm、宽(152.06±1.61)μm,初产时呈白色,后逐渐变为橘黄色,透过卵壳可见2个红色小点;卵期(3.81±0.03)d。若虫共5龄,形态特征差异明显。1龄若虫头比腹宽,无翅芽;2龄若虫翅芽初显、较小,前后翅分离;3龄若虫翅芽明显,前后翅芽前期相接不重叠,后期重叠小部分;4龄若虫后翅被前翅重叠一半;5龄若虫前后翅大部分重叠。1~5龄若虫触角节数分别为3、4、6、8、10节,体长分别为(371.82±4.31)μm、(728.15±36.2)μm、(996.9±34.03)μm、(1271.06±24.65)μm和(2083.33±22.89)μm,发育历期分别为(3.81±0.03)d、(3.47±0.06)d、(2.13±0.06)d、(2.03±0.07)d、(2.46±0.06)d和(4.81±0.06)d。成虫触角均为10节,雌成虫尾部呈尖锥状,雄成虫尾部生殖板上翘,夏型雌成虫与雄成虫发育历期分别为(20.88±0.92)d和(28.29±1.67)d。冬型成虫稍大于夏型成虫,冬型与夏型雌成虫体长分别为(4.04±0.12)mm和(3.85±0.24)mm,雄成虫体长分别为(4.02±0.24)mm和(3.31±0.16)mm。夏型雌成虫产卵前期为(5.60±0.24)d,单雌产卵量为(647.75±52.94)粒,雌成虫产卵高峰期在羽化后的第7~14 d,占总产卵量71.2%。综上所述,槐豆木虱若虫共5龄,各龄期间翅芽的发育、触角节数及体长均有显著变化。成虫寿命及产卵期较长,世代之间存在明显的重叠现象,这些发现对于理解其生命周期和制定防治措施具有重要意义。

关键词：槐豆木虱形态特征发育历期生物学特性繁殖力园林害虫

Effects of nitrogen and phosphorus additions on soil nematode community of soybean farmland

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Soil Ecology Letters 2024年第2期6卷 45-55页

作者： Huiying Zhang Mengyang Tian Meiguang Jiang Jingyuan yang Qi Xu Ying Zhang minglu Ji Yuteng Yao Cancan Zhao Yuan Miao International Joint Research Laboratory for Global Change Ecology School of Life SciencesHenan UniversityKaifeng475004China Henan Dabieshan National Field Observation and Research Station of Forest Ecosystem Xinyang Academy of Ecological ResearchXinyang464000China

The nitrogen(N)and phosphorus(P)addition promotes the abundance of soybean soil nematodes.The addition of nitrogen can alleviate the suppression of phosphorus on nematodes.Phosphorus addition affects nematode abundanc... 详细信息

关键词： soil nematodes nitrogen addition phosphorus addition soil food web soybean

Edaravone-loaded poly(amino acid) nanogel inhibits ferroptosis for neuroprotection in cerebral ischemia injury

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Asian Journal of Pharmaceutical Sciences 2024年第2期19卷 89-101页

作者： Yunhan Zhang Zhulin Zou Shuang Liu Fangfang Chen minglu Li Haoyang Zou Haiyan Liu Jianxun Ding Key Laboratory of Pathobiology Ministry of Education Department of AnatomyCollege of Basic Medical SciencesJilin UniversityChangchun 130061China Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China Department of Gastrointestinal Colorectaland Anal SurgeryChina-Japan Union Hospital of Jilin UniversityChangchun 130033China

Neurological injury caused by ischemic stroke is a major cause of permanent disability and death. The currently available neuroprotective drugs fail to achieve desired therapeutic efficacy mainly due to short circulation half-life and poor blood−brain barrier (BBB) permeability. For that, an edaravone-loaded pH/glutathione (pH/GSH) dual-responsive poly(amino acid) nanogel (NG/EDA) was developed to improve the neuroprotection of EDA. The nanogel was triggered by acidic and EDA-induced high-level GSH microenvironments, which enabled the selective and sustained release of EDA at the site of ischemic injury. NG/EDA exhibited a uniform sub-spherical morphology with a mean hydrodynamic diameter of 112.3 ± 8.2 nm. NG/EDA efficiently accumulated at the cerebral ischemic injury site of permanent middle cerebral artery occlusion (pMCAO) mice, showing an efficient BBB crossing feature. Notably, NG/EDA with 50 µM EDA significantly increased neuron survival (29.3%) following oxygen and glucose deprivation by inhibiting ferroptosis. In addition, administering NG/EDA for 7 d significantly reduced infarct volume to 22.2% ± 7.2% and decreased neurobehavioral scores from 9.0 ± 0.6 to 2.0 ± 0.8. Such a pH/GSH dual-responsive nanoplatform might provide a unique and promising modality for neuroprotection in ischemic stroke and other central nervous system diseases.

关键词： Poly(amino acid)nanogel Controlled drug delivery Inhibition of ferroptosis Neuroprotection Cerebral ischenia injury therapy

Selective nano-buckling improves the performance of graphene logic transistors

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National Science Review 2024年第2期11卷 95-96页

作者： Tong yang Ming yang Department of Applied Physics The Hong Kong Polytechnic University Research Centre for Nanoscience and Nanotechnology The Hong Kong Polytechnic University

The massive integration of silicon-based transistors into modern electronic devices greatly increases power consumption and heat generation/dissipation[1]. In this concern, transistors based on atomically-thin graphene that exhibit high electric and thermoelectric conductivities with negligible contact resistance,

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The neutrophil–osteogenic cell axis promotes bone destruction in periodontitis

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International Journal of Oral Science 2024年第1期16卷 154-162页

作者： Yutaro Ando Masayuki Tsukasaki Nam Cong-Nhat Huynh Shizao Zang minglu Yan Ryunosuke Muro Kazutaka Nakamura Masatsugu Komagamine Noriko Komatsu Kazuo Okamoto Kenta Nakano Tadashi Okamura Akira Yamaguchi Kazuyuki Ishihara Hiroshi Takayanagi Department of Immunology Graduate School of Medicine and Faculty of MedicineThe University of Tokyo7-3-1HongoBunkyo-kuTokyoJapan Department of Microbiology Tokyo Dental College2-1-14 Kanda-Misaki-choChiyoda-kuTokyoJapan Oral Health Science Center Tokyo Dental College2-9-18Kanda-Misaki-choChiyodakuTokyoJapan Department of Osteoimmunology Graduate School of Medicine and Faculty of MedicineThe University of Tokyo7-3-1HongoBunkyo-kuTokyoJapan Unit of Prosthodontics Laboratory of Oral-Maxillofacial Biology Faculty of Odonto-StomatologyUniversity of Medicine and Pharmacy at Ho Chi Minh CityHo Chi Minh CityVietnam Department of Oral and Maxillofacial Surgery Department of Sensory and Motor System MedicineGraduate School of MedicineThe University of TokyoTokyoJapan Division of Rheumatology Department of Internal MedicineKeio University School of MedicineTokyoJapan Department of Laboratory Animal Medicine Research InstituteNational Center for Global Health and MedicineTokyoJapan

The immune-stromal cell interactions play a key role in health and diseases. In periodontitis, the most prevalent infectious disease in humans, immune cells accumulate in the oral mucosa and promote bone destruction by inducing receptor activator of nuclear factor-κB ligand (RANKL) expression in osteogenic cells such as osteoblasts and periodontal ligament cells. However, the detailed mechanism underlying immune–bone cell interactions in periodontitis is not fully understood. Here, we performed single-cell RNAsequencing analysis on mouse periodontal lesions and showed that neutrophil–osteogenic cell crosstalk is involved in periodontitis-induced bone loss. The periodontal lesions displayed marked infiltration of neutrophils, and in silico analyses suggested that the neutrophils interacted with osteogenic cells through cytokine production. Among the cytokines expressed in the periodontal neutrophils, oncostatin M (OSM) potently induced RANKL expression in the primary osteoblasts, and deletion of the OSM receptor in osteogenic cells significantly ameliorated periodontitis-induced bone loss. Epigenomic data analyses identified the OSM-regulated RANKL enhancer region in osteogenic cells, and mice lacking this enhancer showed decreased periodontal bone loss while maintaining physiological bone metabolism. These findings shed light on the role of neutrophils in bone regulation during bacterial infection, highlighting the novel mechanism underlying osteoimmune crosstalk.

关键词： period neutrophil destruction

Deep Insight of Design,Mechanism,and Cancer Theranostic Strategy of Nanozymes

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Nano-Micro Letters 2024年第2期16卷 165-217页

作者： Lu yang Shuming Dong Shili Gai Dan yang He Ding Lili Feng Guixin yang Ziaur Rehman Piaoping yang Key Laboratory of Superlight Materials and Surface Technology Ministry of EducationCollege of Materials Science and Chemical EngineeringHarbin Engineering UniversityHarbin 150001People’s Republic of China Yantai Research Institute Harbin Engineering UniversityYantai 264000People’s Republic of China Key Laboratory of Green Chemical Engineering and Technology of Heilongjiang Province College of Material Science and Chemical EngineeringHarbin University of Science and TechnologyHarbin 150040People’s Republic of China Department of Chemistry Quaid-i-Azam UniversityIslamabad 45320Pakistan

Since the discovery of enzyme-like activity of Fe3O4 nanoparticles in 2007,nanozymes are becoming the promising substitutes for natural enzymes due to their advantages of high catalytic activity,low cost,mild reaction conditions,good stability,and suitable for large-scale production.Recently,with the cross fusion of nanomedicine and nanocatalysis,nanozyme-based theranostic strategies attract great attention,since the enzymatic reactions can be triggered in the tumor microenvironment to achieve good curative effect with substrate specificity and low side effects.Thus,various nanozymes have been developed and used for tumor therapy.In this review,more than 270 research articles are discussed systematically to present progress in the past five years.First,the discovery and development of nanozymes are summarized.Second,classification and catalytic mechanism of nanozymes are discussed.Third,activity prediction and rational design of nanozymes are focused by highlighting the methods of density functional theory,machine learning,biomimetic and chemical design.Then,synergistic theranostic strategy of nanozymes are introduced.Finally,current challenges and future prospects of nanozymes used for tumor theranostic are outlined,including selectivity,biosafety,repeatability and stability,in-depth catalytic mechanism,predicting and evaluating activities.

关键词： Nanozymes Classification Prediction and design Catalytic mechanism Tumor theranostics